Hydroxyl free radicals induce cell differentiation in SK-N-MC neuroblastoma cells
The SK-N-MC cell line is frequently used as a model of neuronal differentiation induced by 5-bromodeoxyuridine (BrdU). In this study, the differentiation properties of this cell line were investigated under hydroxyl free radical generation, and compared to BrdU treatment. Hydroxyl free radicals were...
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| Veröffentlicht in: | Tissue & cell 2002-02, Vol.34 (1), p.33-38 |
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| creator | Oravecz, K. Kalka, D. Jeney, F. Cantz, M. -Nagy, I.Zs |
| description | The SK-N-MC cell line is frequently used as a model of neuronal differentiation induced by 5-bromodeoxyuridine (BrdU). In this study, the differentiation properties of this cell line were investigated under hydroxyl free radical generation, and compared to BrdU treatment. Hydroxyl free radicals were generated in the cultures by the Fenton reaction, i.e. by simultaneous addition of ADP-Fe2+ complex and H2O2. Microscopic morphological signs, as well as the acetylcholinesterase and ganglioside sialidase activities were considered as markers of neuronal differentiation of this cholinergic neuroblastoma cell line. Apart from the altered morphological appearance, the marker enzymes displayed significant increases after both types of intervention. We suggest that hydroxyl free radicals can induce in vitro cell differentiation. They apparently play a more complex role in cell physiology than simply causing oxidative damage. |
| doi_str_mv | 10.1054/tice.2001.0221 |
| format | Article |
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In this study, the differentiation properties of this cell line were investigated under hydroxyl free radical generation, and compared to BrdU treatment. Hydroxyl free radicals were generated in the cultures by the Fenton reaction, i.e. by simultaneous addition of ADP-Fe2+ complex and H2O2. Microscopic morphological signs, as well as the acetylcholinesterase and ganglioside sialidase activities were considered as markers of neuronal differentiation of this cholinergic neuroblastoma cell line. Apart from the altered morphological appearance, the marker enzymes displayed significant increases after both types of intervention. We suggest that hydroxyl free radicals can induce in vitro cell differentiation. 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In this study, the differentiation properties of this cell line were investigated under hydroxyl free radical generation, and compared to BrdU treatment. Hydroxyl free radicals were generated in the cultures by the Fenton reaction, i.e. by simultaneous addition of ADP-Fe2+ complex and H2O2. Microscopic morphological signs, as well as the acetylcholinesterase and ganglioside sialidase activities were considered as markers of neuronal differentiation of this cholinergic neuroblastoma cell line. Apart from the altered morphological appearance, the marker enzymes displayed significant increases after both types of intervention. We suggest that hydroxyl free radicals can induce in vitro cell differentiation. They apparently play a more complex role in cell physiology than simply causing oxidative damage.</abstract><cop>Scotland</cop><pub>Elsevier Ltd</pub><pmid>11989968</pmid><doi>10.1054/tice.2001.0221</doi><tpages>6</tpages></addata></record> |
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| subjects | Acetylcholinesterase - metabolism Adolescent Brain Neoplasms - metabolism Brain Neoplasms - pathology Bromodeoxyuridine Cell Differentiation - physiology Cell Division Cell Survival DNA, Neoplasm - metabolism Humans Hydrogen Peroxide Hydroxyl Radical - metabolism Iron Neoplasm Proteins - metabolism Neuraminidase - metabolism Neuroblastoma - metabolism Neuroblastoma - pathology neuroblastoma, cell differentiation, OH• free radicals, Fenton reaction, acetylcholinesterase, ganglioside sialidase Tumor Cells, Cultured |
| title | Hydroxyl free radicals induce cell differentiation in SK-N-MC neuroblastoma cells |
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