Evaluation of staining techniques, antigen detection and nested PCR for the diagnosis of cryptosporidiosis in HIV seropositive and seronegative patients

The study was designed to determine the efficacy of modified Ziehl–Neelsen (ZN), safranine methylene blue (SM) staining, antigen detection ELISA and a nested PCR assay (specific for Cryptosporidium parvum) for detection of Cryptosporidium in HIV seropositive and seronegative patients with diarrhoea....

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Veröffentlicht in:	Acta tropica 2008, Vol.107 (1), p.1-7
Hauptverfasser:	Kaushik, Kirti, Khurana, Sumeeta, Wanchu, Ajay, Malla, Nancy
Format:	Artikel
Sprache:	eng
Schlagworte:	Adolescent Adult Aged AIDS-Related Opportunistic Infections - parasitology Animals Antigen detection Antigens, Protozoan - immunology Biological and medical sciences Child Child, Preschool Cryptosporidiosis Cryptosporidiosis - diagnosis Cryptosporidium - isolation & purification Cryptosporidium parvum Diarrhea - parasitology DNA, Protozoan - genetics Enzyme-Linked Immunosorbent Assay - methods Female General aspects Human immunodeficiency virus Human protozoal diseases Human viral diseases Humans Infant Infectious diseases Male Medical sciences Middle Aged Parasitic diseases PCR Polymerase Chain Reaction - methods Protozoal diseases Sensitivity and Specificity Staining and Labeling - methods Staining techniques Viral diseases Viral diseases of the lymphoid tissue and the blood. Aids
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