Serum tartrate-resistant acid phosphatase isoforms in rheumatoid arthritis
Objectives: Our objective was to evaluate the significance and source of serum tartrate-resistant acid phosphatase (TRACP) in patients with rheumatoid arthritis (RA). Methods: Thirty-five RA, 32 osteoarthritis (OA) and 16 control subjects were studied. Serum TRACP-5b activity and total TRACP protein...
Gespeichert in:
| Veröffentlicht in: | Clinica chimica acta 2002-06, Vol.320 (1), p.49-58 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 58 |
|---|---|
| container_issue | 1 |
| container_start_page | 49 |
| container_title | Clinica chimica acta |
| container_volume | 320 |
| creator | Janckila, Anthony J. Neustadt, David H. Nakasato, Yuri R. Halleen, Jussi M. Hentunen, Teuvo Yam, Lung T. |
| description | Objectives: Our objective was to evaluate the significance and source of serum tartrate-resistant acid phosphatase (TRACP) in patients with rheumatoid arthritis (RA).
Methods: Thirty-five RA, 32 osteoarthritis (OA) and 16 control subjects were studied. Serum TRACP-5b activity and total TRACP protein were determined by immunoassay. TRACP isoforms were analyzed by non-denaturing polyacrylamide gel electrophoresis (PAGE). Serum bone alkaline phosphatase (BAP), cross-linked N-terminal telopeptides (NTx), and C-terminal telopeptides (ICTP) of type I collagen were estimated as markers of bone turnover. C-reactive protein (CRP) was measured as a marker of chronic inflammation. Macrophages and dendritic cells (DC) were developed from peripheral blood monocytes. Cell lysates and culture supernatants were analyzed for TRACP isoforms by immunoassay and PAGE.
Results: In RA, mean TRACP-5b activity was normal, but median total TRACP protein was increased twofold (
p |
| doi_str_mv | 10.1016/S0009-8981(02)00026-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71642451</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0009898102000268</els_id><sourcerecordid>71642451</sourcerecordid><originalsourceid>FETCH-LOGICAL-c391t-7c1cadb8083040d57d509e9ebca29e444b453024c92b83a4384623d61cdb769f3</originalsourceid><addsrcrecordid>eNqFkEtLLDEQRoMoOj5-gpfeKLpozau7k5WI-ERwoa5DOqlmcpnunptKC_57M85wXboqCs5X9XEIOWb0glFWX75SSnWptGJnlJ_nhdel2iIzphpRCqn5Npn9R_bIPuLfvEpas12yx5hWglM6I0-vEKe-SDamaBOUETBgskMqrAu-WM5HXM5tsghFwLEbY49FGIo4h6m3acxITs5jSAEPyU5nFwhHm3lA3u9u324eyueX-8eb6-fSCc1S2TjmrG8VVSLX8VXjK6pBQ-ss1yClbGUlKJdO81YJK4WSNRe-Zs63Ta07cUBO13eXcfw3ASbTB3SwWNgBxglNw2rJZcUyWK1BF0fECJ1ZxtDb-GkYNSuJ5luiWRkylJtviUbl3J_Ng6ntwf-kNtYycLIBLDq76KIdXMAfTuQCSq-4qzUHWcdHgGjQBRgc-BDBJePH8EuVL2MOjnE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71642451</pqid></control><display><type>article</type><title>Serum tartrate-resistant acid phosphatase isoforms in rheumatoid arthritis</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Janckila, Anthony J. ; Neustadt, David H. ; Nakasato, Yuri R. ; Halleen, Jussi M. ; Hentunen, Teuvo ; Yam, Lung T.</creator><creatorcontrib>Janckila, Anthony J. ; Neustadt, David H. ; Nakasato, Yuri R. ; Halleen, Jussi M. ; Hentunen, Teuvo ; Yam, Lung T.</creatorcontrib><description>Objectives: Our objective was to evaluate the significance and source of serum tartrate-resistant acid phosphatase (TRACP) in patients with rheumatoid arthritis (RA).
Methods: Thirty-five RA, 32 osteoarthritis (OA) and 16 control subjects were studied. Serum TRACP-5b activity and total TRACP protein were determined by immunoassay. TRACP isoforms were analyzed by non-denaturing polyacrylamide gel electrophoresis (PAGE). Serum bone alkaline phosphatase (BAP), cross-linked N-terminal telopeptides (NTx), and C-terminal telopeptides (ICTP) of type I collagen were estimated as markers of bone turnover. C-reactive protein (CRP) was measured as a marker of chronic inflammation. Macrophages and dendritic cells (DC) were developed from peripheral blood monocytes. Cell lysates and culture supernatants were analyzed for TRACP isoforms by immunoassay and PAGE.
Results: In RA, mean TRACP-5b activity was normal, but median total TRACP protein was increased twofold (
p<0.001). In OA, TRACP-5b activity and protein were normal. In RA, TRACP-5b activity correlated weakly with ICTP (
r=0.56) while TRACP protein levels correlated weakly with NTx (
r=0.43). Additionally, TRACP protein, but not TRACP-5b activity correlated significantly with CRP (
r=0.42). Macrophage and DC lysates contained TRACP-5b, while tissue culture supernatants contained TRACP-5a.
Conclusions: Increased total TRACP protein in RA sera was probably due to TRACP-5a and not derived from osteoclasts. Rather, it could be a secreted product of inflammatory macrophages and DC.</description><identifier>ISSN: 0009-8981</identifier><identifier>EISSN: 1873-3492</identifier><identifier>DOI: 10.1016/S0009-8981(02)00026-8</identifier><identifier>PMID: 11983200</identifier><identifier>CODEN: CCATAR</identifier><language>eng</language><publisher>Shannon: Elsevier B.V</publisher><subject>Acid Phosphatase - blood ; Adolescent ; Adult ; Aged ; Aged, 80 and over ; Arthritis, Rheumatoid - blood ; Arthritis, Rheumatoid - enzymology ; Biological and medical sciences ; Biomarkers - blood ; C-Reactive Protein - analysis ; Cells, Cultured ; Dendritic cell ; Dendritic Cells - enzymology ; Diseases of the osteoarticular system ; Electrophoresis, Polyacrylamide Gel ; Humans ; Immunoassay ; Inflammation ; Inflammation - blood ; Inflammation - enzymology ; Inflammatory joint diseases ; Isoenzymes - blood ; Macrophage ; Macrophages - enzymology ; Medical sciences ; Middle Aged ; Osteoarthritis - blood ; Osteoarthritis - enzymology ; Rheumatoid arthritis ; Sensitivity and Specificity ; Tartrate-Resistant Acid Phosphatase</subject><ispartof>Clinica chimica acta, 2002-06, Vol.320 (1), p.49-58</ispartof><rights>2002 Elsevier Science B.V.</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-7c1cadb8083040d57d509e9ebca29e444b453024c92b83a4384623d61cdb769f3</citedby><cites>FETCH-LOGICAL-c391t-7c1cadb8083040d57d509e9ebca29e444b453024c92b83a4384623d61cdb769f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0009-8981(02)00026-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13642890$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11983200$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Janckila, Anthony J.</creatorcontrib><creatorcontrib>Neustadt, David H.</creatorcontrib><creatorcontrib>Nakasato, Yuri R.</creatorcontrib><creatorcontrib>Halleen, Jussi M.</creatorcontrib><creatorcontrib>Hentunen, Teuvo</creatorcontrib><creatorcontrib>Yam, Lung T.</creatorcontrib><title>Serum tartrate-resistant acid phosphatase isoforms in rheumatoid arthritis</title><title>Clinica chimica acta</title><addtitle>Clin Chim Acta</addtitle><description>Objectives: Our objective was to evaluate the significance and source of serum tartrate-resistant acid phosphatase (TRACP) in patients with rheumatoid arthritis (RA).
Methods: Thirty-five RA, 32 osteoarthritis (OA) and 16 control subjects were studied. Serum TRACP-5b activity and total TRACP protein were determined by immunoassay. TRACP isoforms were analyzed by non-denaturing polyacrylamide gel electrophoresis (PAGE). Serum bone alkaline phosphatase (BAP), cross-linked N-terminal telopeptides (NTx), and C-terminal telopeptides (ICTP) of type I collagen were estimated as markers of bone turnover. C-reactive protein (CRP) was measured as a marker of chronic inflammation. Macrophages and dendritic cells (DC) were developed from peripheral blood monocytes. Cell lysates and culture supernatants were analyzed for TRACP isoforms by immunoassay and PAGE.
Results: In RA, mean TRACP-5b activity was normal, but median total TRACP protein was increased twofold (
p<0.001). In OA, TRACP-5b activity and protein were normal. In RA, TRACP-5b activity correlated weakly with ICTP (
r=0.56) while TRACP protein levels correlated weakly with NTx (
r=0.43). Additionally, TRACP protein, but not TRACP-5b activity correlated significantly with CRP (
r=0.42). Macrophage and DC lysates contained TRACP-5b, while tissue culture supernatants contained TRACP-5a.
Conclusions: Increased total TRACP protein in RA sera was probably due to TRACP-5a and not derived from osteoclasts. Rather, it could be a secreted product of inflammatory macrophages and DC.</description><subject>Acid Phosphatase - blood</subject><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Arthritis, Rheumatoid - blood</subject><subject>Arthritis, Rheumatoid - enzymology</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - blood</subject><subject>C-Reactive Protein - analysis</subject><subject>Cells, Cultured</subject><subject>Dendritic cell</subject><subject>Dendritic Cells - enzymology</subject><subject>Diseases of the osteoarticular system</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Humans</subject><subject>Immunoassay</subject><subject>Inflammation</subject><subject>Inflammation - blood</subject><subject>Inflammation - enzymology</subject><subject>Inflammatory joint diseases</subject><subject>Isoenzymes - blood</subject><subject>Macrophage</subject><subject>Macrophages - enzymology</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Osteoarthritis - blood</subject><subject>Osteoarthritis - enzymology</subject><subject>Rheumatoid arthritis</subject><subject>Sensitivity and Specificity</subject><subject>Tartrate-Resistant Acid Phosphatase</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLLDEQRoMoOj5-gpfeKLpozau7k5WI-ERwoa5DOqlmcpnunptKC_57M85wXboqCs5X9XEIOWb0glFWX75SSnWptGJnlJ_nhdel2iIzphpRCqn5Npn9R_bIPuLfvEpas12yx5hWglM6I0-vEKe-SDamaBOUETBgskMqrAu-WM5HXM5tsghFwLEbY49FGIo4h6m3acxITs5jSAEPyU5nFwhHm3lA3u9u324eyueX-8eb6-fSCc1S2TjmrG8VVSLX8VXjK6pBQ-ss1yClbGUlKJdO81YJK4WSNRe-Zs63Ta07cUBO13eXcfw3ASbTB3SwWNgBxglNw2rJZcUyWK1BF0fECJ1ZxtDb-GkYNSuJ5luiWRkylJtviUbl3J_Ng6ntwf-kNtYycLIBLDq76KIdXMAfTuQCSq-4qzUHWcdHgGjQBRgc-BDBJePH8EuVL2MOjnE</recordid><startdate>20020601</startdate><enddate>20020601</enddate><creator>Janckila, Anthony J.</creator><creator>Neustadt, David H.</creator><creator>Nakasato, Yuri R.</creator><creator>Halleen, Jussi M.</creator><creator>Hentunen, Teuvo</creator><creator>Yam, Lung T.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020601</creationdate><title>Serum tartrate-resistant acid phosphatase isoforms in rheumatoid arthritis</title><author>Janckila, Anthony J. ; Neustadt, David H. ; Nakasato, Yuri R. ; Halleen, Jussi M. ; Hentunen, Teuvo ; Yam, Lung T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-7c1cadb8083040d57d509e9ebca29e444b453024c92b83a4384623d61cdb769f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Acid Phosphatase - blood</topic><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Arthritis, Rheumatoid - blood</topic><topic>Arthritis, Rheumatoid - enzymology</topic><topic>Biological and medical sciences</topic><topic>Biomarkers - blood</topic><topic>C-Reactive Protein - analysis</topic><topic>Cells, Cultured</topic><topic>Dendritic cell</topic><topic>Dendritic Cells - enzymology</topic><topic>Diseases of the osteoarticular system</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Humans</topic><topic>Immunoassay</topic><topic>Inflammation</topic><topic>Inflammation - blood</topic><topic>Inflammation - enzymology</topic><topic>Inflammatory joint diseases</topic><topic>Isoenzymes - blood</topic><topic>Macrophage</topic><topic>Macrophages - enzymology</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Osteoarthritis - blood</topic><topic>Osteoarthritis - enzymology</topic><topic>Rheumatoid arthritis</topic><topic>Sensitivity and Specificity</topic><topic>Tartrate-Resistant Acid Phosphatase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Janckila, Anthony J.</creatorcontrib><creatorcontrib>Neustadt, David H.</creatorcontrib><creatorcontrib>Nakasato, Yuri R.</creatorcontrib><creatorcontrib>Halleen, Jussi M.</creatorcontrib><creatorcontrib>Hentunen, Teuvo</creatorcontrib><creatorcontrib>Yam, Lung T.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Janckila, Anthony J.</au><au>Neustadt, David H.</au><au>Nakasato, Yuri R.</au><au>Halleen, Jussi M.</au><au>Hentunen, Teuvo</au><au>Yam, Lung T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serum tartrate-resistant acid phosphatase isoforms in rheumatoid arthritis</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>2002-06-01</date><risdate>2002</risdate><volume>320</volume><issue>1</issue><spage>49</spage><epage>58</epage><pages>49-58</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><coden>CCATAR</coden><abstract>Objectives: Our objective was to evaluate the significance and source of serum tartrate-resistant acid phosphatase (TRACP) in patients with rheumatoid arthritis (RA).
Methods: Thirty-five RA, 32 osteoarthritis (OA) and 16 control subjects were studied. Serum TRACP-5b activity and total TRACP protein were determined by immunoassay. TRACP isoforms were analyzed by non-denaturing polyacrylamide gel electrophoresis (PAGE). Serum bone alkaline phosphatase (BAP), cross-linked N-terminal telopeptides (NTx), and C-terminal telopeptides (ICTP) of type I collagen were estimated as markers of bone turnover. C-reactive protein (CRP) was measured as a marker of chronic inflammation. Macrophages and dendritic cells (DC) were developed from peripheral blood monocytes. Cell lysates and culture supernatants were analyzed for TRACP isoforms by immunoassay and PAGE.
Results: In RA, mean TRACP-5b activity was normal, but median total TRACP protein was increased twofold (
p<0.001). In OA, TRACP-5b activity and protein were normal. In RA, TRACP-5b activity correlated weakly with ICTP (
r=0.56) while TRACP protein levels correlated weakly with NTx (
r=0.43). Additionally, TRACP protein, but not TRACP-5b activity correlated significantly with CRP (
r=0.42). Macrophage and DC lysates contained TRACP-5b, while tissue culture supernatants contained TRACP-5a.
Conclusions: Increased total TRACP protein in RA sera was probably due to TRACP-5a and not derived from osteoclasts. Rather, it could be a secreted product of inflammatory macrophages and DC.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>11983200</pmid><doi>10.1016/S0009-8981(02)00026-8</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0009-8981 |
| ispartof | Clinica chimica acta, 2002-06, Vol.320 (1), p.49-58 |
| issn | 0009-8981 1873-3492 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71642451 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Acid Phosphatase - blood Adolescent Adult Aged Aged, 80 and over Arthritis, Rheumatoid - blood Arthritis, Rheumatoid - enzymology Biological and medical sciences Biomarkers - blood C-Reactive Protein - analysis Cells, Cultured Dendritic cell Dendritic Cells - enzymology Diseases of the osteoarticular system Electrophoresis, Polyacrylamide Gel Humans Immunoassay Inflammation Inflammation - blood Inflammation - enzymology Inflammatory joint diseases Isoenzymes - blood Macrophage Macrophages - enzymology Medical sciences Middle Aged Osteoarthritis - blood Osteoarthritis - enzymology Rheumatoid arthritis Sensitivity and Specificity Tartrate-Resistant Acid Phosphatase |
| title | Serum tartrate-resistant acid phosphatase isoforms in rheumatoid arthritis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T22%3A52%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Serum%20tartrate-resistant%20acid%20phosphatase%20isoforms%20in%20rheumatoid%20arthritis&rft.jtitle=Clinica%20chimica%20acta&rft.au=Janckila,%20Anthony%20J.&rft.date=2002-06-01&rft.volume=320&rft.issue=1&rft.spage=49&rft.epage=58&rft.pages=49-58&rft.issn=0009-8981&rft.eissn=1873-3492&rft.coden=CCATAR&rft_id=info:doi/10.1016/S0009-8981(02)00026-8&rft_dat=%3Cproquest_cross%3E71642451%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=71642451&rft_id=info:pmid/11983200&rft_els_id=S0009898102000268&rfr_iscdi=true |