Identification of the cadBA operon from Vibrio vulnificus and its influence on survival to acid stress
By a transposon-tagging method, cadBA genes encoding a lysine/cadaverin antiporter and a lysine decarboxylase were identified and cloned from Vibrio vulnificus. The deduced amino acid sequences of cadBA were 64-97% similar to those reported from other Enterobacteriaceae. Functions of cadBA genes on...
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| Veröffentlicht in: | FEMS microbiology letters 2002-03, Vol.208 (2), p.245-251 |
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| creator | Rhee, J.E Rhee, J.H Ryu, P.Y Choi, S.H |
| description | By a transposon-tagging method, cadBA genes encoding a lysine/cadaverin antiporter and a lysine decarboxylase were identified and cloned from Vibrio vulnificus. The deduced amino acid sequences of cadBA were 64-97% similar to those reported from other Enterobacteriaceae. Functions of cadBA genes on acid tolerance were assessed by comparing acid tolerances of V. vulnificus and its isogenic mutants, whose cadBA genes were separately inactivated by allelic exchanges. The results demonstrated that gene products of cadBA contribute to acid tolerance of V. vulnificus, and that their contribution is dependent on prior exposure of cells to moderately acidic pH. |
| doi_str_mv | 10.1111/j.1574-6968.2002.tb11089.x |
| format | Article |
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The deduced amino acid sequences of cadBA were 64-97% similar to those reported from other Enterobacteriaceae. Functions of cadBA genes on acid tolerance were assessed by comparing acid tolerances of V. vulnificus and its isogenic mutants, whose cadBA genes were separately inactivated by allelic exchanges. The results demonstrated that gene products of cadBA contribute to acid tolerance of V. vulnificus, and that their contribution is dependent on prior exposure of cells to moderately acidic pH.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.2002.tb11089.x</identifier><identifier>PMID: 11959444</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Acid tolerance ; amino acid sequences ; Amino Acid Transport Systems ; Amino acids ; antiporters ; Antiporters - genetics ; Antiporters - physiology ; Bacterial diseases ; Bacterial diseases of the digestive system and abdomen ; Bacterial Proteins - genetics ; Bacterial Proteins - physiology ; Bacteriology ; Biological and medical sciences ; cadA gene ; cadB gene ; Carboxy-Lyases - genetics ; Carboxy-Lyases - metabolism ; Chromosome Mapping ; DNA, Bacterial - analysis ; Enterobacteriaceae ; Fundamental and applied biological sciences. Psychology ; Genes ; Genetics ; Human bacterial diseases ; Hydrogen-Ion Concentration ; Infectious diseases ; Lysine ; Lysine decarboxylase ; lysine/cadaverin antiporter ; Medical sciences ; Microbiology ; Models, Genetic ; Molecular Sequence Data ; mutants ; Mutation ; Operon ; Sequence Analysis, DNA ; Tolerances ; Vibrio - genetics ; Vibrio - growth & development ; Vibrio vulnificus ; Vibrio vulnificus cadBA ; Waterborne diseases</subject><ispartof>FEMS microbiology letters, 2002-03, Vol.208 (2), p.245-251</ispartof><rights>2002 Federation of European Microbiological Societies 2002</rights><rights>2002 Federation of European Microbiological Societies</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4615-c886f5397c51dffae3f3d7800d7525e07cc5f4019f5ad844efe43ecc43c9532e3</citedby><cites>FETCH-LOGICAL-c4615-c886f5397c51dffae3f3d7800d7525e07cc5f4019f5ad844efe43ecc43c9532e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6968.2002.tb11089.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6968.2002.tb11089.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14261010$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11959444$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rhee, J.E</creatorcontrib><creatorcontrib>Rhee, J.H</creatorcontrib><creatorcontrib>Ryu, P.Y</creatorcontrib><creatorcontrib>Choi, S.H</creatorcontrib><title>Identification of the cadBA operon from Vibrio vulnificus and its influence on survival to acid stress</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>By a transposon-tagging method, cadBA genes encoding a lysine/cadaverin antiporter and a lysine decarboxylase were identified and cloned from Vibrio vulnificus. The deduced amino acid sequences of cadBA were 64-97% similar to those reported from other Enterobacteriaceae. Functions of cadBA genes on acid tolerance were assessed by comparing acid tolerances of V. vulnificus and its isogenic mutants, whose cadBA genes were separately inactivated by allelic exchanges. The results demonstrated that gene products of cadBA contribute to acid tolerance of V. vulnificus, and that their contribution is dependent on prior exposure of cells to moderately acidic pH.</description><subject>Acid tolerance</subject><subject>amino acid sequences</subject><subject>Amino Acid Transport Systems</subject><subject>Amino acids</subject><subject>antiporters</subject><subject>Antiporters - genetics</subject><subject>Antiporters - physiology</subject><subject>Bacterial diseases</subject><subject>Bacterial diseases of the digestive system and abdomen</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - physiology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>cadA gene</subject><subject>cadB gene</subject><subject>Carboxy-Lyases - genetics</subject><subject>Carboxy-Lyases - metabolism</subject><subject>Chromosome Mapping</subject><subject>DNA, Bacterial - analysis</subject><subject>Enterobacteriaceae</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genetics</subject><subject>Human bacterial diseases</subject><subject>Hydrogen-Ion Concentration</subject><subject>Infectious diseases</subject><subject>Lysine</subject><subject>Lysine decarboxylase</subject><subject>lysine/cadaverin antiporter</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Models, Genetic</subject><subject>Molecular Sequence Data</subject><subject>mutants</subject><subject>Mutation</subject><subject>Operon</subject><subject>Sequence Analysis, DNA</subject><subject>Tolerances</subject><subject>Vibrio - genetics</subject><subject>Vibrio - growth & development</subject><subject>Vibrio vulnificus</subject><subject>Vibrio vulnificus cadBA</subject><subject>Waterborne diseases</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqVkV2L1DAUhoMo7rj6FzQoetfx5LOpF8K6uLow4oWutyGTJpqh04xJO-7-e1NaXBBFzE0ged6Tc_Ig9JTAmpT1crcmouaVbKRaUwC6HraEgGrW13fQ6tfVXbQCVquKQFOfoAc57wCAU5D30QkhjWg45yvkL1vXD8EHa4YQexw9Hr45bE375gzHg0vlzKe4x1_CNoWIj2PXT_SYselbHIaMQ--70fXW4cLmMR3D0XR4iNjY0OI8JJfzQ3TPmy67R8t-iq4u3n4-f19tPr67PD_bVJZLIiqrlPSCNbUVpPXeOOZZWyuAthZUOKitFZ4DabwwreLceceZs5Yz2whGHTtFL-a6hxS_jy4Peh-ydV1nehfHrGsiOWWU_RMkigHjjSzgs9_AXRxTX4bQlBGQNVWKFurVTNkUc07O60MKe5NuNAE9SdM7PZnRkxk9SdOLNH1dwo-XJ8bt3rW30cVSAZ4vgMnWdD6Z3oZ8y3EqCRAo3OuZ-xE6d_MfLeiLDxvKRSkg5gJxPPwlXv15gidzzpuozddUmrv6RIFIAFJ-Ukj2EzYgzME</recordid><startdate>20020305</startdate><enddate>20020305</enddate><creator>Rhee, J.E</creator><creator>Rhee, J.H</creator><creator>Ryu, P.Y</creator><creator>Choi, S.H</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20020305</creationdate><title>Identification of the cadBA operon from Vibrio vulnificus and its influence on survival to acid stress</title><author>Rhee, J.E ; Rhee, J.H ; Ryu, P.Y ; Choi, S.H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4615-c886f5397c51dffae3f3d7800d7525e07cc5f4019f5ad844efe43ecc43c9532e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Acid tolerance</topic><topic>amino acid sequences</topic><topic>Amino Acid Transport Systems</topic><topic>Amino acids</topic><topic>antiporters</topic><topic>Antiporters - genetics</topic><topic>Antiporters - physiology</topic><topic>Bacterial diseases</topic><topic>Bacterial diseases of the digestive system and abdomen</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - physiology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>cadA gene</topic><topic>cadB gene</topic><topic>Carboxy-Lyases - genetics</topic><topic>Carboxy-Lyases - metabolism</topic><topic>Chromosome Mapping</topic><topic>DNA, Bacterial - analysis</topic><topic>Enterobacteriaceae</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Genetics</topic><topic>Human bacterial diseases</topic><topic>Hydrogen-Ion Concentration</topic><topic>Infectious diseases</topic><topic>Lysine</topic><topic>Lysine decarboxylase</topic><topic>lysine/cadaverin antiporter</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Models, Genetic</topic><topic>Molecular Sequence Data</topic><topic>mutants</topic><topic>Mutation</topic><topic>Operon</topic><topic>Sequence Analysis, DNA</topic><topic>Tolerances</topic><topic>Vibrio - genetics</topic><topic>Vibrio - growth & development</topic><topic>Vibrio vulnificus</topic><topic>Vibrio vulnificus cadBA</topic><topic>Waterborne diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rhee, J.E</creatorcontrib><creatorcontrib>Rhee, J.H</creatorcontrib><creatorcontrib>Ryu, P.Y</creatorcontrib><creatorcontrib>Choi, S.H</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest Health & Medical Research Collection</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health & Nursing</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Applied & Life Sciences</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rhee, J.E</au><au>Rhee, J.H</au><au>Ryu, P.Y</au><au>Choi, S.H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of the cadBA operon from Vibrio vulnificus and its influence on survival to acid stress</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2002-03-05</date><risdate>2002</risdate><volume>208</volume><issue>2</issue><spage>245</spage><epage>251</epage><pages>245-251</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>By a transposon-tagging method, cadBA genes encoding a lysine/cadaverin antiporter and a lysine decarboxylase were identified and cloned from Vibrio vulnificus. The deduced amino acid sequences of cadBA were 64-97% similar to those reported from other Enterobacteriaceae. Functions of cadBA genes on acid tolerance were assessed by comparing acid tolerances of V. vulnificus and its isogenic mutants, whose cadBA genes were separately inactivated by allelic exchanges. The results demonstrated that gene products of cadBA contribute to acid tolerance of V. vulnificus, and that their contribution is dependent on prior exposure of cells to moderately acidic pH.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>11959444</pmid><doi>10.1111/j.1574-6968.2002.tb11089.x</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 2002-03, Vol.208 (2), p.245-251 |
| issn | 0378-1097 1574-6968 |
| language | eng |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection |
| subjects | Acid tolerance amino acid sequences Amino Acid Transport Systems Amino acids antiporters Antiporters - genetics Antiporters - physiology Bacterial diseases Bacterial diseases of the digestive system and abdomen Bacterial Proteins - genetics Bacterial Proteins - physiology Bacteriology Biological and medical sciences cadA gene cadB gene Carboxy-Lyases - genetics Carboxy-Lyases - metabolism Chromosome Mapping DNA, Bacterial - analysis Enterobacteriaceae Fundamental and applied biological sciences. Psychology Genes Genetics Human bacterial diseases Hydrogen-Ion Concentration Infectious diseases Lysine Lysine decarboxylase lysine/cadaverin antiporter Medical sciences Microbiology Models, Genetic Molecular Sequence Data mutants Mutation Operon Sequence Analysis, DNA Tolerances Vibrio - genetics Vibrio - growth & development Vibrio vulnificus Vibrio vulnificus cadBA Waterborne diseases |
| title | Identification of the cadBA operon from Vibrio vulnificus and its influence on survival to acid stress |
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