A CLN2-Related and Thermostable Serine-Carboxyl Proteinase, Kumamolysin: Cloning, Expression, and Identification of Catalytic Serine Residue

The gene encoding kumamolysin, a thermostable pepstatin-insensitive carboxyl proteinase, was cloned and expressed, (i) Kumamolysin was synthesized as a large precursor consisting of two regions: amino-terminal prepro (188 amino acids) and mature proteins (384 amino acids), (ii) The deduced amino aci...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of biochemistry (Tokyo) 2002-05, Vol.131 (5), p.757-765
Hauptverfasser:	Oyama, Hiroshi, Hamada, Takatoshi, Ogasawara, Shin, Uchida, Kenichi, Murao, Sawao, Beyer, Bret B., Dunn, Ben M., Oda, Kohei
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Aspartic Acid Endopeptidases - antagonists & inhibitors Aspartic Acid Endopeptidases - chemistry Aspartic Acid Endopeptidases - genetics Aspartic Acid Endopeptidases - metabolism Base Sequence Blotting, Western Catalytic Domain catalytric triad Cloning, Molecular Enzyme Stability Escherichia coli - enzymology Hot Temperature Kinetics kumamoysin Molecular Sequence Data Mutation pepstatin-insensitive carboxyl proteinase Protease Inhibitors - pharmacology Recombinant Proteins - antagonists & inhibitors Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Restriction Mapping Sequence Homology Serine - metabolism serine-carboxyl proteinase Structure-Activity Relationship thermostable proteinase
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!