Glucose-Induced Time-Dependent Potentiation of Insulin Release, but not Islet Blood Perfusion, in Anesthetized Rats
Background: Repeated administration of glucose in vivo leads to a time-dependent potentiation of insulin release. Glucose is also known to stimulate pancreatic islet blood flow, but whether this is associated with a time-dependent potentiation is unknown. We therefore repeatedly administered glucose...
Gespeichert in:
| Veröffentlicht in: | Upsala journal of medical sciences 2008, Vol.113 (1), p.47-56 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 56 |
|---|---|
| container_issue | 1 |
| container_start_page | 47 |
| container_title | Upsala journal of medical sciences |
| container_volume | 113 |
| creator | Jansson, Leif Bodin, Birgitta Källskog, Örjan |
| description | Background: Repeated administration of glucose in vivo leads to a time-dependent potentiation of insulin release. Glucose is also known to stimulate pancreatic islet blood flow, but whether this is associated with a time-dependent potentiation is unknown. We therefore repeatedly administered glucose to anesthetized rats and evaluated effects on insulin release and islet blood flow.
Methods: Male Wistar-Furth rats, anesthetized with thiobutabarbital, were injected intravenously with 1 ml of saline or glucose at times 0, 30 and 60 min. The combinations used were saline + saline + saline (SSS), glucose + saline + saline (GSS), saline + saline + glucose (SSG) and glucose + glucose + glucose (GGG). Regional organ blood flow values were measured 3 min after the final injection with a microsphere technique, and at this time also serum insulin concentrations were determined with ELISA.
Results: Serum insulin concentrations as well as total pancreatic, pancreatic islet and duodenal blood flow were higher in SSG and GGG-treated rats when compared to those given SSS and GSS. However, only insulin concentrations, not blood flow values, were higher in GGG rats when compared to SSG animals.
Conclusions: Glucose-induced time-dependent potentiation of insulin release occurs in vivo in thiobutabarbital-anesthetized rats, but is not associated with a further increase in islet blood flow. |
| doi_str_mv | 10.3109/2000-1967-213 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_infor</sourceid><recordid>TN_cdi_proquest_miscellaneous_71635696</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>71635696</sourcerecordid><originalsourceid>FETCH-LOGICAL-c381t-5e4c333bd0f70c6c410c73c0f58d15c2dabfb5f406a5dd7fb6e40c422a9df4f03</originalsourceid><addsrcrecordid>eNp1kU1rGzEQhkVpaNw0x16LTj15U2m1n0c3SVNDICGkuQqtNKoVtJKrD0Ly6ytjk1JoTzODnnkY9CL0kZIzRsn4pSaEVHTs-qqm7A1avM5v0YKw0o89a47R-xgfy0tHevYOHdOhrWk_DgsUr2yWPkK1dipLUPjezFBdwBacApfwrU-lGJGMd9hrvHYxW-PwHVgQEZZ4ygk7n_A6Wkj4q_Ve4VsIOseyscQFXTmIaQPJvBT9nUjxAzrSwkY4PdQT9OPb5f359-r65mp9vrquJBtoqlpoJGNsUkT3RHayoUT2TBLdDoq2slZi0lOrG9KJVqleTx00RDZ1LUalG03YCVruvfEJtnni22BmEZ65F4ZfmIcV9-Enz5lTQtp2KPjnPb4N_lcuN_PZRAnWCgc-R97TjrXd2BWw2oMy-BgD6FczJXwXCt-FwHch8BJK4T8dxHmaQf2hDykUYNgDxmkfZvHkg1U8iWfrgw7CSRN33n-7-79WNyBs2kgRgD_6HFz53_9c9Rs5WazR</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71635696</pqid></control><display><type>article</type><title>Glucose-Induced Time-Dependent Potentiation of Insulin Release, but not Islet Blood Perfusion, in Anesthetized Rats</title><source>Taylor & Francis</source><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Jansson, Leif ; Bodin, Birgitta ; Källskog, Örjan</creator><creatorcontrib>Jansson, Leif ; Bodin, Birgitta ; Källskog, Örjan</creatorcontrib><description>Background: Repeated administration of glucose in vivo leads to a time-dependent potentiation of insulin release. Glucose is also known to stimulate pancreatic islet blood flow, but whether this is associated with a time-dependent potentiation is unknown. We therefore repeatedly administered glucose to anesthetized rats and evaluated effects on insulin release and islet blood flow.
Methods: Male Wistar-Furth rats, anesthetized with thiobutabarbital, were injected intravenously with 1 ml of saline or glucose at times 0, 30 and 60 min. The combinations used were saline + saline + saline (SSS), glucose + saline + saline (GSS), saline + saline + glucose (SSG) and glucose + glucose + glucose (GGG). Regional organ blood flow values were measured 3 min after the final injection with a microsphere technique, and at this time also serum insulin concentrations were determined with ELISA.
Results: Serum insulin concentrations as well as total pancreatic, pancreatic islet and duodenal blood flow were higher in SSG and GGG-treated rats when compared to those given SSS and GSS. However, only insulin concentrations, not blood flow values, were higher in GGG rats when compared to SSG animals.
Conclusions: Glucose-induced time-dependent potentiation of insulin release occurs in vivo in thiobutabarbital-anesthetized rats, but is not associated with a further increase in islet blood flow.</description><identifier>ISSN: 0300-9734</identifier><identifier>ISSN: 2000-1967</identifier><identifier>EISSN: 2000-1967</identifier><identifier>DOI: 10.3109/2000-1967-213</identifier><identifier>PMID: 18521798</identifier><language>eng</language><publisher>England: Informa UK Ltd</publisher><subject>Animals ; Blood Glucose - analysis ; Glucose - administration & dosage ; Glucose - pharmacology ; Insulin - blood ; Insulin - metabolism ; Islets of Langerhans - blood supply ; Islets of Langerhans - drug effects ; Islets of Langerhans - metabolism ; Male ; MEDICIN ; MEDICINE ; Rats ; Rats, Wistar</subject><ispartof>Upsala journal of medical sciences, 2008, Vol.113 (1), p.47-56</ispartof><rights>2008 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 2008</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c381t-5e4c333bd0f70c6c410c73c0f58d15c2dabfb5f406a5dd7fb6e40c422a9df4f03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.3109/2000-1967-213$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.3109/2000-1967-213$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>230,314,776,780,860,881,4010,27900,27901,27902,59620,60409,61194,61375</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18521798$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-100558$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Jansson, Leif</creatorcontrib><creatorcontrib>Bodin, Birgitta</creatorcontrib><creatorcontrib>Källskog, Örjan</creatorcontrib><title>Glucose-Induced Time-Dependent Potentiation of Insulin Release, but not Islet Blood Perfusion, in Anesthetized Rats</title><title>Upsala journal of medical sciences</title><addtitle>Ups J Med Sci</addtitle><description>Background: Repeated administration of glucose in vivo leads to a time-dependent potentiation of insulin release. Glucose is also known to stimulate pancreatic islet blood flow, but whether this is associated with a time-dependent potentiation is unknown. We therefore repeatedly administered glucose to anesthetized rats and evaluated effects on insulin release and islet blood flow.
Methods: Male Wistar-Furth rats, anesthetized with thiobutabarbital, were injected intravenously with 1 ml of saline or glucose at times 0, 30 and 60 min. The combinations used were saline + saline + saline (SSS), glucose + saline + saline (GSS), saline + saline + glucose (SSG) and glucose + glucose + glucose (GGG). Regional organ blood flow values were measured 3 min after the final injection with a microsphere technique, and at this time also serum insulin concentrations were determined with ELISA.
Results: Serum insulin concentrations as well as total pancreatic, pancreatic islet and duodenal blood flow were higher in SSG and GGG-treated rats when compared to those given SSS and GSS. However, only insulin concentrations, not blood flow values, were higher in GGG rats when compared to SSG animals.
Conclusions: Glucose-induced time-dependent potentiation of insulin release occurs in vivo in thiobutabarbital-anesthetized rats, but is not associated with a further increase in islet blood flow.</description><subject>Animals</subject><subject>Blood Glucose - analysis</subject><subject>Glucose - administration & dosage</subject><subject>Glucose - pharmacology</subject><subject>Insulin - blood</subject><subject>Insulin - metabolism</subject><subject>Islets of Langerhans - blood supply</subject><subject>Islets of Langerhans - drug effects</subject><subject>Islets of Langerhans - metabolism</subject><subject>Male</subject><subject>MEDICIN</subject><subject>MEDICINE</subject><subject>Rats</subject><subject>Rats, Wistar</subject><issn>0300-9734</issn><issn>2000-1967</issn><issn>2000-1967</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU1rGzEQhkVpaNw0x16LTj15U2m1n0c3SVNDICGkuQqtNKoVtJKrD0Ly6ytjk1JoTzODnnkY9CL0kZIzRsn4pSaEVHTs-qqm7A1avM5v0YKw0o89a47R-xgfy0tHevYOHdOhrWk_DgsUr2yWPkK1dipLUPjezFBdwBacApfwrU-lGJGMd9hrvHYxW-PwHVgQEZZ4ygk7n_A6Wkj4q_Ve4VsIOseyscQFXTmIaQPJvBT9nUjxAzrSwkY4PdQT9OPb5f359-r65mp9vrquJBtoqlpoJGNsUkT3RHayoUT2TBLdDoq2slZi0lOrG9KJVqleTx00RDZ1LUalG03YCVruvfEJtnni22BmEZ65F4ZfmIcV9-Enz5lTQtp2KPjnPb4N_lcuN_PZRAnWCgc-R97TjrXd2BWw2oMy-BgD6FczJXwXCt-FwHch8BJK4T8dxHmaQf2hDykUYNgDxmkfZvHkg1U8iWfrgw7CSRN33n-7-79WNyBs2kgRgD_6HFz53_9c9Rs5WazR</recordid><startdate>2008</startdate><enddate>2008</enddate><creator>Jansson, Leif</creator><creator>Bodin, Birgitta</creator><creator>Källskog, Örjan</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>DF2</scope></search><sort><creationdate>2008</creationdate><title>Glucose-Induced Time-Dependent Potentiation of Insulin Release, but not Islet Blood Perfusion, in Anesthetized Rats</title><author>Jansson, Leif ; Bodin, Birgitta ; Källskog, Örjan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-5e4c333bd0f70c6c410c73c0f58d15c2dabfb5f406a5dd7fb6e40c422a9df4f03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Blood Glucose - analysis</topic><topic>Glucose - administration & dosage</topic><topic>Glucose - pharmacology</topic><topic>Insulin - blood</topic><topic>Insulin - metabolism</topic><topic>Islets of Langerhans - blood supply</topic><topic>Islets of Langerhans - drug effects</topic><topic>Islets of Langerhans - metabolism</topic><topic>Male</topic><topic>MEDICIN</topic><topic>MEDICINE</topic><topic>Rats</topic><topic>Rats, Wistar</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jansson, Leif</creatorcontrib><creatorcontrib>Bodin, Birgitta</creatorcontrib><creatorcontrib>Källskog, Örjan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Uppsala universitet</collection><jtitle>Upsala journal of medical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jansson, Leif</au><au>Bodin, Birgitta</au><au>Källskog, Örjan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glucose-Induced Time-Dependent Potentiation of Insulin Release, but not Islet Blood Perfusion, in Anesthetized Rats</atitle><jtitle>Upsala journal of medical sciences</jtitle><addtitle>Ups J Med Sci</addtitle><date>2008</date><risdate>2008</risdate><volume>113</volume><issue>1</issue><spage>47</spage><epage>56</epage><pages>47-56</pages><issn>0300-9734</issn><issn>2000-1967</issn><eissn>2000-1967</eissn><abstract>Background: Repeated administration of glucose in vivo leads to a time-dependent potentiation of insulin release. Glucose is also known to stimulate pancreatic islet blood flow, but whether this is associated with a time-dependent potentiation is unknown. We therefore repeatedly administered glucose to anesthetized rats and evaluated effects on insulin release and islet blood flow.
Methods: Male Wistar-Furth rats, anesthetized with thiobutabarbital, were injected intravenously with 1 ml of saline or glucose at times 0, 30 and 60 min. The combinations used were saline + saline + saline (SSS), glucose + saline + saline (GSS), saline + saline + glucose (SSG) and glucose + glucose + glucose (GGG). Regional organ blood flow values were measured 3 min after the final injection with a microsphere technique, and at this time also serum insulin concentrations were determined with ELISA.
Results: Serum insulin concentrations as well as total pancreatic, pancreatic islet and duodenal blood flow were higher in SSG and GGG-treated rats when compared to those given SSS and GSS. However, only insulin concentrations, not blood flow values, were higher in GGG rats when compared to SSG animals.
Conclusions: Glucose-induced time-dependent potentiation of insulin release occurs in vivo in thiobutabarbital-anesthetized rats, but is not associated with a further increase in islet blood flow.</abstract><cop>England</cop><pub>Informa UK Ltd</pub><pmid>18521798</pmid><doi>10.3109/2000-1967-213</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0300-9734 |
| ispartof | Upsala journal of medical sciences, 2008, Vol.113 (1), p.47-56 |
| issn | 0300-9734 2000-1967 2000-1967 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71635696 |
| source | Taylor & Francis; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Blood Glucose - analysis Glucose - administration & dosage Glucose - pharmacology Insulin - blood Insulin - metabolism Islets of Langerhans - blood supply Islets of Langerhans - drug effects Islets of Langerhans - metabolism Male MEDICIN MEDICINE Rats Rats, Wistar |
| title | Glucose-Induced Time-Dependent Potentiation of Insulin Release, but not Islet Blood Perfusion, in Anesthetized Rats |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T04%3A38%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_infor&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Glucose-Induced%20Time-Dependent%20Potentiation%20of%20Insulin%20Release,%20but%20not%20Islet%20Blood%20Perfusion,%20in%20Anesthetized%20Rats&rft.jtitle=Upsala%20journal%20of%20medical%20sciences&rft.au=Jansson,%20Leif&rft.date=2008&rft.volume=113&rft.issue=1&rft.spage=47&rft.epage=56&rft.pages=47-56&rft.issn=0300-9734&rft.eissn=2000-1967&rft_id=info:doi/10.3109/2000-1967-213&rft_dat=%3Cproquest_infor%3E71635696%3C/proquest_infor%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=71635696&rft_id=info:pmid/18521798&rfr_iscdi=true |