Aeromonas hydrophila vacuolating activity in the Caco-2 human enterocyte cell line as a putative virulence factor
Abstract A non-enterotoxigenic strain of Aeromonas hydrophila isolated from diarrheic stools of an 8-month-old child was found to cause vacuolation in Caco-2 cells. The vacuoles became prominent 60 min after addition of the bacterial culture to the cell monolayers and, after 120 min, a complete disr...
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| Veröffentlicht in: | FEMS microbiology letters 2002-02, Vol.207 (2), p.127-131 |
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| container_title | FEMS microbiology letters |
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| creator | Guimarães, Márcia S Andrade, João R.C. Freitas-Almeida, Ângela C Ferreira, Maria Cândida S |
| description | Abstract
A non-enterotoxigenic strain of Aeromonas hydrophila isolated from diarrheic stools of an 8-month-old child was found to cause vacuolation in Caco-2 cells. The vacuoles became prominent 60 min after addition of the bacterial culture to the cell monolayers and, after 120 min, a complete disruption of the monolayers was observed. Cell vacuolation was not detected when Caco-2 monolayers were tested with sterile filtrates of overnight cultures of the A. hydrophila vacuolating strain AH14846. This strain produced a diffuse adherence pattern in Caco-2 cell monolayers, but did not produce detectable cytotonic enterotoxin in the suckling mouse test and only produced small quantities of aerolysin. By demonstrating the ability to induce vacuolation in mammalian cells of enterocytic lineage, the current study raises the possibility that such activity might contribute to gastrointestinal symptoms in infections involving Aeromonas strains which do not express well-established enterotoxins. |
| doi_str_mv | 10.1111/j.1574-6968.2002.tb11040.x |
| format | Article |
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A non-enterotoxigenic strain of Aeromonas hydrophila isolated from diarrheic stools of an 8-month-old child was found to cause vacuolation in Caco-2 cells. The vacuoles became prominent 60 min after addition of the bacterial culture to the cell monolayers and, after 120 min, a complete disruption of the monolayers was observed. Cell vacuolation was not detected when Caco-2 monolayers were tested with sterile filtrates of overnight cultures of the A. hydrophila vacuolating strain AH14846. This strain produced a diffuse adherence pattern in Caco-2 cell monolayers, but did not produce detectable cytotonic enterotoxin in the suckling mouse test and only produced small quantities of aerolysin. By demonstrating the ability to induce vacuolation in mammalian cells of enterocytic lineage, the current study raises the possibility that such activity might contribute to gastrointestinal symptoms in infections involving Aeromonas strains which do not express well-established enterotoxins.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.2002.tb11040.x</identifier><identifier>PMID: 11958929</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Aeromonas hydrophila ; Aeromonas hydrophila - isolation & purification ; Aeromonas hydrophila - pathogenicity ; Aeromonas hydrophila - physiology ; Bacterial diseases ; Bacterial diseases of the digestive system and abdomen ; Bacteriology ; Biological and medical sciences ; Caco-2 Cells - microbiology ; Caco-2 Cells - pathology ; Caco‐2 cell ; Cell culture ; Diarrhea - microbiology ; Disruption ; Enterotoxins ; Fundamental and applied biological sciences. Psychology ; Gastrointestinal symptoms ; Hemolysin Proteins - analysis ; Human bacterial diseases ; Humans ; Infectious diseases ; Mammalian cells ; Medical sciences ; Microbiology ; Monolayers ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; Suckling behavior ; Vacuolating activity ; Vacuoles ; Vacuoles - pathology ; Virulence ; Virulence factors</subject><ispartof>FEMS microbiology letters, 2002-02, Vol.207 (2), p.127-131</ispartof><rights>2002 Federation of European Microbiological Societies 2002</rights><rights>2002 Federation of European Microbiological Societies</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4837-d50071f8e592e48182fb5b237ee4f8911fbc1b41275c8bd714875ec6540623de3</citedby><cites>FETCH-LOGICAL-c4837-d50071f8e592e48182fb5b237ee4f8911fbc1b41275c8bd714875ec6540623de3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6968.2002.tb11040.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6968.2002.tb11040.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,782,786,1419,27933,27934,45583,45584</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14253305$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11958929$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guimarães, Márcia S</creatorcontrib><creatorcontrib>Andrade, João R.C.</creatorcontrib><creatorcontrib>Freitas-Almeida, Ângela C</creatorcontrib><creatorcontrib>Ferreira, Maria Cândida S</creatorcontrib><title>Aeromonas hydrophila vacuolating activity in the Caco-2 human enterocyte cell line as a putative virulence factor</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Abstract
A non-enterotoxigenic strain of Aeromonas hydrophila isolated from diarrheic stools of an 8-month-old child was found to cause vacuolation in Caco-2 cells. The vacuoles became prominent 60 min after addition of the bacterial culture to the cell monolayers and, after 120 min, a complete disruption of the monolayers was observed. Cell vacuolation was not detected when Caco-2 monolayers were tested with sterile filtrates of overnight cultures of the A. hydrophila vacuolating strain AH14846. This strain produced a diffuse adherence pattern in Caco-2 cell monolayers, but did not produce detectable cytotonic enterotoxin in the suckling mouse test and only produced small quantities of aerolysin. By demonstrating the ability to induce vacuolation in mammalian cells of enterocytic lineage, the current study raises the possibility that such activity might contribute to gastrointestinal symptoms in infections involving Aeromonas strains which do not express well-established enterotoxins.</description><subject>Aeromonas hydrophila</subject><subject>Aeromonas hydrophila - isolation & purification</subject><subject>Aeromonas hydrophila - pathogenicity</subject><subject>Aeromonas hydrophila - physiology</subject><subject>Bacterial diseases</subject><subject>Bacterial diseases of the digestive system and abdomen</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Caco-2 Cells - microbiology</subject><subject>Caco-2 Cells - pathology</subject><subject>Caco‐2 cell</subject><subject>Cell culture</subject><subject>Diarrhea - microbiology</subject><subject>Disruption</subject><subject>Enterotoxins</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gastrointestinal symptoms</subject><subject>Hemolysin Proteins - analysis</subject><subject>Human bacterial diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Mammalian cells</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Monolayers</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>Suckling behavior</subject><subject>Vacuolating activity</subject><subject>Vacuoles</subject><subject>Vacuoles - pathology</subject><subject>Virulence</subject><subject>Virulence factors</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqVkUuP0zAURiMEYjoDfwFZIGaXcq8fscMCaVQxgFTEAlhbjutQV2ncsZMy_fc4akUlxEN4cxc-333oFMVzhDnm92ozRyF5WdWVmlMAOh8aROAwv39QzH5-PSxmwKQqEWp5UVymtAEATqF6XFwg1kLVtJ4Vdzcuhm3oTSLrwyqG3dp3huyNHUNnBt9_I8YOfu-HA_E9GdaOLIwNJSXrcWt64voh5-1hcMS6riOd7x3JvQzZjUPO7x3Z-zh2rreOtLlViE-KR63pknt6qlfF19u3Xxbvy-Wndx8WN8vScsVkuRIAElvlRE0dV6ho24iGMukcb1WN2DYWG45UCqualUSupHC2EhwqylaOXRXXx767GO5Glwa99Wla0vQujElLrJjAGv8J5tGKAqsy-OIXcBPG2OcjNGUIlWS84pl6faRsDClF1-pd9FsTDxpBT_70Rk-S9CRJT_70yZ--z2HxlzBgpT9PUvUkVQNqAEGlnoY-O602Nlu3OqdOojPw8gSYZE3XRtNbn84cp4IxEJl7c-S--84d_mN1fftxmWWcLwjj7g_x8neX_wDsANch</recordid><startdate>20020205</startdate><enddate>20020205</enddate><creator>Guimarães, Márcia S</creator><creator>Andrade, João R.C.</creator><creator>Freitas-Almeida, Ângela C</creator><creator>Ferreira, Maria Cândida S</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20020205</creationdate><title>Aeromonas hydrophila vacuolating activity in the Caco-2 human enterocyte cell line as a putative virulence factor</title><author>Guimarães, Márcia S ; Andrade, João R.C. ; Freitas-Almeida, Ângela C ; Ferreira, Maria Cândida S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4837-d50071f8e592e48182fb5b237ee4f8911fbc1b41275c8bd714875ec6540623de3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Aeromonas hydrophila</topic><topic>Aeromonas hydrophila - isolation & purification</topic><topic>Aeromonas hydrophila - pathogenicity</topic><topic>Aeromonas hydrophila - physiology</topic><topic>Bacterial diseases</topic><topic>Bacterial diseases of the digestive system and abdomen</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Caco-2 Cells - microbiology</topic><topic>Caco-2 Cells - pathology</topic><topic>Caco‐2 cell</topic><topic>Cell culture</topic><topic>Diarrhea - microbiology</topic><topic>Disruption</topic><topic>Enterotoxins</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gastrointestinal symptoms</topic><topic>Hemolysin Proteins - analysis</topic><topic>Human bacterial diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Mammalian cells</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Monolayers</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>Suckling behavior</topic><topic>Vacuolating activity</topic><topic>Vacuoles</topic><topic>Vacuoles - pathology</topic><topic>Virulence</topic><topic>Virulence factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guimarães, Márcia S</creatorcontrib><creatorcontrib>Andrade, João R.C.</creatorcontrib><creatorcontrib>Freitas-Almeida, Ângela C</creatorcontrib><creatorcontrib>Ferreira, Maria Cândida S</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guimarães, Márcia S</au><au>Andrade, João R.C.</au><au>Freitas-Almeida, Ângela C</au><au>Ferreira, Maria Cândida S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Aeromonas hydrophila vacuolating activity in the Caco-2 human enterocyte cell line as a putative virulence factor</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2002-02-05</date><risdate>2002</risdate><volume>207</volume><issue>2</issue><spage>127</spage><epage>131</epage><pages>127-131</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Abstract
A non-enterotoxigenic strain of Aeromonas hydrophila isolated from diarrheic stools of an 8-month-old child was found to cause vacuolation in Caco-2 cells. The vacuoles became prominent 60 min after addition of the bacterial culture to the cell monolayers and, after 120 min, a complete disruption of the monolayers was observed. Cell vacuolation was not detected when Caco-2 monolayers were tested with sterile filtrates of overnight cultures of the A. hydrophila vacuolating strain AH14846. This strain produced a diffuse adherence pattern in Caco-2 cell monolayers, but did not produce detectable cytotonic enterotoxin in the suckling mouse test and only produced small quantities of aerolysin. By demonstrating the ability to induce vacuolation in mammalian cells of enterocytic lineage, the current study raises the possibility that such activity might contribute to gastrointestinal symptoms in infections involving Aeromonas strains which do not express well-established enterotoxins.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>11958929</pmid><doi>10.1111/j.1574-6968.2002.tb11040.x</doi><tpages>5</tpages></addata></record> |
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| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 2002-02, Vol.207 (2), p.127-131 |
| issn | 0378-1097 1574-6968 |
| language | eng |
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| source | MEDLINE; Access via Wiley Online Library; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Aeromonas hydrophila Aeromonas hydrophila - isolation & purification Aeromonas hydrophila - pathogenicity Aeromonas hydrophila - physiology Bacterial diseases Bacterial diseases of the digestive system and abdomen Bacteriology Biological and medical sciences Caco-2 Cells - microbiology Caco-2 Cells - pathology Caco‐2 cell Cell culture Diarrhea - microbiology Disruption Enterotoxins Fundamental and applied biological sciences. Psychology Gastrointestinal symptoms Hemolysin Proteins - analysis Human bacterial diseases Humans Infectious diseases Mammalian cells Medical sciences Microbiology Monolayers Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Suckling behavior Vacuolating activity Vacuoles Vacuoles - pathology Virulence Virulence factors |
| title | Aeromonas hydrophila vacuolating activity in the Caco-2 human enterocyte cell line as a putative virulence factor |
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