Internal and external contamination of donor corneas before in situ excision: bacterial risk factors in 93 donors
Most studies of corneal donor contamination concentrate on postenucleation contamination of the eyeball. The purpose of the present study was to evaluate the relative contamination of in situ excised corneal tissue and relevance to final success or rejection by recipients of the corneal grafts. Nine...
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| Veröffentlicht in: | Albrecht von Graefe s Archiv für Ophthalmologie 2002-04, Vol.240 (4), p.265-270 |
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| creator | ROBERT, Pierre-Yves CAMEZIND, Philippe DROUET, Mireille PLOY, Marie-Cécile ADENIS, Jean-Paul |
| description | Most studies of corneal donor contamination concentrate on postenucleation contamination of the eyeball. The purpose of the present study was to evaluate the relative contamination of in situ excised corneal tissue and relevance to final success or rejection by recipients of the corneal grafts.
Ninety-three donors underwent anterior chamber puncture (ACP) and corneal epithelium scarification (CS) before and after disinfection with 5% povidone-iodine. Following in situ excision, corneas were preserved in culture medium at +35 degrees C. Morphological and bacteriological assessment was carried out after culture, and recipients were followed up over a 2-year period.
Samples taken by ACP, CS before disinfection, CS after disinfection and a culture medium sample were contaminated by bacteria in, respectively, 8 (8.6%), 23 (24.7%) 4 (4.3%) and 5 (5.4%) donors. Contamination of aqueous humor was significantly associated with age, death-to-sample time and premortem systemic infection. Contamination of epithelium significantly increased culture medium contamination.
External bacteria on donor cornea are mainly skin bacteria (especially Staphylococcus) and can be partially eliminated by a povidone-iodine wash. Internal bacteria are mainly gut bacteria and may be due to perimortem bacteriemia. However, bacterial infection at the time of death appears to have no effect on the incidence of endophthalmitis in recipients and should no longer prevent use of such corneal tissue in grafts. |
| doi_str_mv | 10.1007/s004170100322 |
| format | Article |
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Ninety-three donors underwent anterior chamber puncture (ACP) and corneal epithelium scarification (CS) before and after disinfection with 5% povidone-iodine. Following in situ excision, corneas were preserved in culture medium at +35 degrees C. Morphological and bacteriological assessment was carried out after culture, and recipients were followed up over a 2-year period.
Samples taken by ACP, CS before disinfection, CS after disinfection and a culture medium sample were contaminated by bacteria in, respectively, 8 (8.6%), 23 (24.7%) 4 (4.3%) and 5 (5.4%) donors. Contamination of aqueous humor was significantly associated with age, death-to-sample time and premortem systemic infection. Contamination of epithelium significantly increased culture medium contamination.
External bacteria on donor cornea are mainly skin bacteria (especially Staphylococcus) and can be partially eliminated by a povidone-iodine wash. Internal bacteria are mainly gut bacteria and may be due to perimortem bacteriemia. However, bacterial infection at the time of death appears to have no effect on the incidence of endophthalmitis in recipients and should no longer prevent use of such corneal tissue in grafts.</description><identifier>ISSN: 0721-832X</identifier><identifier>EISSN: 1435-702X</identifier><identifier>DOI: 10.1007/s004170100322</identifier><identifier>PMID: 11981639</identifier><identifier>CODEN: GACODL</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Aqueous Humor ; Aqueous Humor - microbiology ; Bacteria ; Bacteria - isolation & purification ; Biological and medical sciences ; Cornea ; Corneal Transplantation ; Culture Media ; Disinfection ; Disinfection - methods ; Endophthalmitis ; Endophthalmitis - microbiology ; Epithelium, Corneal ; Epithelium, Corneal - microbiology ; Female ; Graft Rejection ; Graft Rejection - microbiology ; Humans ; Immunology ; Life Sciences ; Male ; Medical sciences ; Middle Aged ; Ophthalmology ; Povidone-Iodine ; Risk Factors ; Sclera ; Sclera - microbiology ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Surgery of the eye and orbit ; Tissue Donors ; Tissue Preservation</subject><ispartof>Albrecht von Graefe s Archiv für Ophthalmologie, 2002-04, Vol.240 (4), p.265-270</ispartof><rights>2002 INIST-CNRS</rights><rights>Springer-Verlag 2002</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c380t-124984264d5d8cff9ff8a1bfd553a78ba2c0e76d8c93a8867e9a73b806a85d3f3</citedby><orcidid>0000-0002-0718-4979</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13622503$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11981639$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00023824$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>ROBERT, Pierre-Yves</creatorcontrib><creatorcontrib>CAMEZIND, Philippe</creatorcontrib><creatorcontrib>DROUET, Mireille</creatorcontrib><creatorcontrib>PLOY, Marie-Cécile</creatorcontrib><creatorcontrib>ADENIS, Jean-Paul</creatorcontrib><title>Internal and external contamination of donor corneas before in situ excision: bacterial risk factors in 93 donors</title><title>Albrecht von Graefe s Archiv für Ophthalmologie</title><addtitle>Graefes Arch Clin Exp Ophthalmol</addtitle><description>Most studies of corneal donor contamination concentrate on postenucleation contamination of the eyeball. The purpose of the present study was to evaluate the relative contamination of in situ excised corneal tissue and relevance to final success or rejection by recipients of the corneal grafts.
Ninety-three donors underwent anterior chamber puncture (ACP) and corneal epithelium scarification (CS) before and after disinfection with 5% povidone-iodine. Following in situ excision, corneas were preserved in culture medium at +35 degrees C. Morphological and bacteriological assessment was carried out after culture, and recipients were followed up over a 2-year period.
Samples taken by ACP, CS before disinfection, CS after disinfection and a culture medium sample were contaminated by bacteria in, respectively, 8 (8.6%), 23 (24.7%) 4 (4.3%) and 5 (5.4%) donors. Contamination of aqueous humor was significantly associated with age, death-to-sample time and premortem systemic infection. Contamination of epithelium significantly increased culture medium contamination.
External bacteria on donor cornea are mainly skin bacteria (especially Staphylococcus) and can be partially eliminated by a povidone-iodine wash. Internal bacteria are mainly gut bacteria and may be due to perimortem bacteriemia. However, bacterial infection at the time of death appears to have no effect on the incidence of endophthalmitis in recipients and should no longer prevent use of such corneal tissue in grafts.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Aqueous Humor</subject><subject>Aqueous Humor - microbiology</subject><subject>Bacteria</subject><subject>Bacteria - isolation & purification</subject><subject>Biological and medical sciences</subject><subject>Cornea</subject><subject>Corneal Transplantation</subject><subject>Culture Media</subject><subject>Disinfection</subject><subject>Disinfection - methods</subject><subject>Endophthalmitis</subject><subject>Endophthalmitis - microbiology</subject><subject>Epithelium, Corneal</subject><subject>Epithelium, Corneal - microbiology</subject><subject>Female</subject><subject>Graft Rejection</subject><subject>Graft Rejection - microbiology</subject><subject>Humans</subject><subject>Immunology</subject><subject>Life Sciences</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Ophthalmology</subject><subject>Povidone-Iodine</subject><subject>Risk Factors</subject><subject>Sclera</subject><subject>Sclera - microbiology</subject><subject>Surgery (general aspects). 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Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Surgery of the eye and orbit</topic><topic>Tissue Donors</topic><topic>Tissue Preservation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ROBERT, Pierre-Yves</creatorcontrib><creatorcontrib>CAMEZIND, Philippe</creatorcontrib><creatorcontrib>DROUET, Mireille</creatorcontrib><creatorcontrib>PLOY, Marie-Cécile</creatorcontrib><creatorcontrib>ADENIS, Jean-Paul</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Albrecht von Graefe s Archiv für Ophthalmologie</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ROBERT, Pierre-Yves</au><au>CAMEZIND, Philippe</au><au>DROUET, Mireille</au><au>PLOY, Marie-Cécile</au><au>ADENIS, Jean-Paul</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Internal and external contamination of donor corneas before in situ excision: bacterial risk factors in 93 donors</atitle><jtitle>Albrecht von Graefe s Archiv für Ophthalmologie</jtitle><addtitle>Graefes Arch Clin Exp Ophthalmol</addtitle><date>2002-04-01</date><risdate>2002</risdate><volume>240</volume><issue>4</issue><spage>265</spage><epage>270</epage><pages>265-270</pages><issn>0721-832X</issn><eissn>1435-702X</eissn><coden>GACODL</coden><abstract>Most studies of corneal donor contamination concentrate on postenucleation contamination of the eyeball. The purpose of the present study was to evaluate the relative contamination of in situ excised corneal tissue and relevance to final success or rejection by recipients of the corneal grafts.
Ninety-three donors underwent anterior chamber puncture (ACP) and corneal epithelium scarification (CS) before and after disinfection with 5% povidone-iodine. Following in situ excision, corneas were preserved in culture medium at +35 degrees C. Morphological and bacteriological assessment was carried out after culture, and recipients were followed up over a 2-year period.
Samples taken by ACP, CS before disinfection, CS after disinfection and a culture medium sample were contaminated by bacteria in, respectively, 8 (8.6%), 23 (24.7%) 4 (4.3%) and 5 (5.4%) donors. Contamination of aqueous humor was significantly associated with age, death-to-sample time and premortem systemic infection. Contamination of epithelium significantly increased culture medium contamination.
External bacteria on donor cornea are mainly skin bacteria (especially Staphylococcus) and can be partially eliminated by a povidone-iodine wash. Internal bacteria are mainly gut bacteria and may be due to perimortem bacteriemia. However, bacterial infection at the time of death appears to have no effect on the incidence of endophthalmitis in recipients and should no longer prevent use of such corneal tissue in grafts.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>11981639</pmid><doi>10.1007/s004170100322</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-0718-4979</orcidid></addata></record> |
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| subjects | Adult Aged Aged, 80 and over Aqueous Humor Aqueous Humor - microbiology Bacteria Bacteria - isolation & purification Biological and medical sciences Cornea Corneal Transplantation Culture Media Disinfection Disinfection - methods Endophthalmitis Endophthalmitis - microbiology Epithelium, Corneal Epithelium, Corneal - microbiology Female Graft Rejection Graft Rejection - microbiology Humans Immunology Life Sciences Male Medical sciences Middle Aged Ophthalmology Povidone-Iodine Risk Factors Sclera Sclera - microbiology Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Surgery of the eye and orbit Tissue Donors Tissue Preservation |
| title | Internal and external contamination of donor corneas before in situ excision: bacterial risk factors in 93 donors |
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