Genetic analysis of Dengue virus type 3 isolated in Buenos Aires, Argentina
Dengue virus as a member of mosquito-borne flaviviruses is responsible for an increasing number of human infections worldwide, mainly in tropical and subtropical urban areas. The agent, a single-stranded positive sense RNA virus, is comprised of four serotypes and genetic variation within each serot...
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Veröffentlicht in: | Virus research 2008-07, Vol.135 (1), p.83-88 |
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description | Dengue virus as a member of mosquito-borne flaviviruses is responsible for an increasing number of human infections worldwide, mainly in tropical and subtropical urban areas. The agent, a single-stranded positive sense RNA virus, is comprised of four serotypes and genetic variation within each serotype can be further divided into different genotypes. Dengue outbreaks were reported in bordering countries during the last years; the latest reported in Paraguay in 2006–2007. In Buenos Aires, 32 dengue cases were confirmed in travelers coming from this country by anti-dengue IgM antibodies detection, RT-PCR and/or isolation in C6/36 cell line. Structural proteins C, prM/M, E and non-structural proteins 1 and 2 from eight viruses were genetically characterized. Phylogenetic inference was performed for the E-protein and all viruses clustered with dengue virus 3 Genotype III. This is the first report of genetic characterization of dengue virus 3 in Argentina. |
doi_str_mv | 10.1016/j.virusres.2008.02.013 |
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The agent, a single-stranded positive sense RNA virus, is comprised of four serotypes and genetic variation within each serotype can be further divided into different genotypes. Dengue outbreaks were reported in bordering countries during the last years; the latest reported in Paraguay in 2006–2007. In Buenos Aires, 32 dengue cases were confirmed in travelers coming from this country by anti-dengue IgM antibodies detection, RT-PCR and/or isolation in C6/36 cell line. Structural proteins C, prM/M, E and non-structural proteins 1 and 2 from eight viruses were genetically characterized. Phylogenetic inference was performed for the E-protein and all viruses clustered with dengue virus 3 Genotype III. 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Mistchenko, Alicia Susana</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c397t-35a01a131351317dba8518edca918c9e70a1b9d32e2e87393ec944cb8d9391583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aedes</topic><topic>Aged</topic><topic>Animals</topic><topic>Antibodies, Viral - blood</topic><topic>Argentina - epidemiology</topic><topic>Cell Line</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Dengue</topic><topic>Dengue - epidemiology</topic><topic>Dengue - virology</topic><topic>Dengue virus</topic><topic>Dengue Virus - classification</topic><topic>Dengue Virus - genetics</topic><topic>Dengue Virus - immunology</topic><topic>Dengue Virus - isolation & purification</topic><topic>Dengue virus type 3</topic><topic>Disease Outbreaks</topic><topic>Female</topic><topic>Humans</topic><topic>Immunoglobulin M - blood</topic><topic>Infant</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Molecular epidemiology</topic><topic>Molecular sequence data</topic><topic>Phylogeny</topic><topic>Viral Proteins - chemistry</topic><topic>Viral Proteins - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Barrero, Paola Roxana</creatorcontrib><creatorcontrib>Mistchenko, Alicia Susana</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virus research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Barrero, Paola Roxana</au><au>Mistchenko, Alicia Susana</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic analysis of Dengue virus type 3 isolated in Buenos Aires, Argentina</atitle><jtitle>Virus research</jtitle><addtitle>Virus Res</addtitle><date>2008-07-01</date><risdate>2008</risdate><volume>135</volume><issue>1</issue><spage>83</spage><epage>88</epage><pages>83-88</pages><issn>0168-1702</issn><eissn>1872-7492</eissn><abstract>Dengue virus as a member of mosquito-borne flaviviruses is responsible for an increasing number of human infections worldwide, mainly in tropical and subtropical urban areas. 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subjects | Adolescent Adult Aedes Aged Animals Antibodies, Viral - blood Argentina - epidemiology Cell Line Child Child, Preschool Dengue Dengue - epidemiology Dengue - virology Dengue virus Dengue Virus - classification Dengue Virus - genetics Dengue Virus - immunology Dengue Virus - isolation & purification Dengue virus type 3 Disease Outbreaks Female Humans Immunoglobulin M - blood Infant Male Middle Aged Molecular epidemiology Molecular sequence data Phylogeny Viral Proteins - chemistry Viral Proteins - genetics |
title | Genetic analysis of Dengue virus type 3 isolated in Buenos Aires, Argentina |
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