ECSA/DPPA2 is an Embryo-Cancer Antigen that Is Coexpressed with Cancer-Testis Antigens in Non–Small Cell Lung Cancer
Purpose: Cancer cells recapitulate many behaviors of pluripotent embryonic cells such as unlimited proliferation, and the capacity to self-renew and to migrate. Embryo-cancer sequence A ( ECSA ), later named developmental pluripotency associated-2 ( DPPA2 ), is an embryonic gene initially isolated f...
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| Veröffentlicht in: | Clinical cancer research 2008-06, Vol.14 (11), p.3291-3298 |
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| creator | JOHN, Thomas CABALLERO, Otavia L DAVIS, Lan D ALTORKI, Nasser SIMPSON, Andrew J CHEN, Yao-Tseng MONK, Marilyn CEBON, Jonathan S SVOBODOVA, Suzanne J KONG, Alan CHUA, Ramon BROWNING, Judy FORTUNATO, Sheila DEB, Siddhartha HSU, Melinda GEDYE, Craig A |
| description | Purpose: Cancer cells recapitulate many behaviors of pluripotent embryonic cells such as unlimited proliferation, and the capacity
to self-renew and to migrate. Embryo-cancer sequence A ( ECSA ), later named developmental pluripotency associated-2 ( DPPA2 ), is an embryonic gene initially isolated from pluripotent human preimplantation embryos. We hypothesized that ECSA/DPPA2
would be quiescent in most normal tissues but expressed in cancers and may therefore be a useful target for immunotherapy.
Experimental Design: ECSA/DPPA 2 expression was examined in a panel of normal and tumor tissue by reverse transcription PCR, quantitative real-time PCR,
and immunohistochemistry. A panel of 110 non–small cell lung cancers (NSCLC) were further investigated for the presence of
ECSA/DPPA2 transcripts and several cancer testis antigens (CTA). Sera from 104 patients were analyzed for spontaneous ECSA/DPPA2 antibody production by ELISA and Western blot.
Results: ECSA/DPPA 2 transcripts were limited to normal testis, placenta, bone marrow, thymus, and kidney but expressed in a variety of tumors
most notably in 30% of NSCLC. Enrichment for CTAs in ECSA/DPPA2 -positive NSCLC was observed. Immunohistochemistry confirmed nuclear and cytoplasmic localization in subpopulations of cells
with coexpression of the CTA MAGE-A3. Antibodies to recombinant ECSA/DPPA2 protein were detected in the sera of 4 of 104 patients
with NSCLC but not in healthy controls.
Conclusions: The restricted expression in normal tissues, expression in tumors with coexpression of CTAs, and spontaneous immunogenicity
indicate that ECSA/DPPA 2 is a promising target for antigen-specific immunotherapy in NSCLC. |
| doi_str_mv | 10.1158/1078-0432.CCR-07-1322 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71624006</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>71624006</sourcerecordid><originalsourceid>FETCH-LOGICAL-c470t-a9676c8ab8a1e1fd62e3d0fda8ebea2af46b27004a892899fb96bc6b2a0eece33</originalsourceid><addsrcrecordid>eNpFkMFO3DAURa2qVaGUTwB500osDLbjxMlylA4FaVRQgbX14rxMXE2Swc6Usus_9A_5EjxMChvbss59vj6EHAl-KkSanwmuc8ZVIk_L8ifjmolEyndkX6SpZonM0vfx_J_ZI59C-MW5UIKrj2RP5KkodJruk9_z8mZ29u36eiapCxR6Ou8q_ziwEnqLns760S2xp2MLI70MtBzwz9pjCFjTBze2dMexWwxjzE94oK6nP4b-6e-_mw5WK1piXBabfjnxn8mHBlYBD6f9gNydz2_LC7a4-n5ZzhbMKs1HBkWmM5tDlYNA0dSZxKTmTQ05VggSGpVVUnOuIC9kXhRNVWSVjXfAES0myQH5upu79sP9JnY0nQs2loEeh00wWmRScZ5FMN2B1g8heGzM2rsO_KMR3GyFm61Ms5VponDDtdkKj7nj6YFN1WH9lpoMR-DLBECwsGp8_L8Lr5zkqhBxfuROdlzrlu2D82jsi6noGsHb1ggVe5hEFiJ5BpXil9Y</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71624006</pqid></control><display><type>article</type><title>ECSA/DPPA2 is an Embryo-Cancer Antigen that Is Coexpressed with Cancer-Testis Antigens in Non–Small Cell Lung Cancer</title><source>MEDLINE</source><source>American Association for Cancer Research</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>JOHN, Thomas ; CABALLERO, Otavia L ; DAVIS, Lan D ; ALTORKI, Nasser ; SIMPSON, Andrew J ; CHEN, Yao-Tseng ; MONK, Marilyn ; CEBON, Jonathan S ; SVOBODOVA, Suzanne J ; KONG, Alan ; CHUA, Ramon ; BROWNING, Judy ; FORTUNATO, Sheila ; DEB, Siddhartha ; HSU, Melinda ; GEDYE, Craig A</creator><creatorcontrib>JOHN, Thomas ; CABALLERO, Otavia L ; DAVIS, Lan D ; ALTORKI, Nasser ; SIMPSON, Andrew J ; CHEN, Yao-Tseng ; MONK, Marilyn ; CEBON, Jonathan S ; SVOBODOVA, Suzanne J ; KONG, Alan ; CHUA, Ramon ; BROWNING, Judy ; FORTUNATO, Sheila ; DEB, Siddhartha ; HSU, Melinda ; GEDYE, Craig A</creatorcontrib><description>Purpose: Cancer cells recapitulate many behaviors of pluripotent embryonic cells such as unlimited proliferation, and the capacity
to self-renew and to migrate. Embryo-cancer sequence A ( ECSA ), later named developmental pluripotency associated-2 ( DPPA2 ), is an embryonic gene initially isolated from pluripotent human preimplantation embryos. We hypothesized that ECSA/DPPA2
would be quiescent in most normal tissues but expressed in cancers and may therefore be a useful target for immunotherapy.
Experimental Design: ECSA/DPPA 2 expression was examined in a panel of normal and tumor tissue by reverse transcription PCR, quantitative real-time PCR,
and immunohistochemistry. A panel of 110 non–small cell lung cancers (NSCLC) were further investigated for the presence of
ECSA/DPPA2 transcripts and several cancer testis antigens (CTA). Sera from 104 patients were analyzed for spontaneous ECSA/DPPA2 antibody production by ELISA and Western blot.
Results: ECSA/DPPA 2 transcripts were limited to normal testis, placenta, bone marrow, thymus, and kidney but expressed in a variety of tumors
most notably in 30% of NSCLC. Enrichment for CTAs in ECSA/DPPA2 -positive NSCLC was observed. Immunohistochemistry confirmed nuclear and cytoplasmic localization in subpopulations of cells
with coexpression of the CTA MAGE-A3. Antibodies to recombinant ECSA/DPPA2 protein were detected in the sera of 4 of 104 patients
with NSCLC but not in healthy controls.
Conclusions: The restricted expression in normal tissues, expression in tumors with coexpression of CTAs, and spontaneous immunogenicity
indicate that ECSA/DPPA 2 is a promising target for antigen-specific immunotherapy in NSCLC.</description><identifier>ISSN: 1078-0432</identifier><identifier>EISSN: 1557-3265</identifier><identifier>DOI: 10.1158/1078-0432.CCR-07-1322</identifier><identifier>PMID: 18519755</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>Antigens, Neoplasm - biosynthesis ; Antineoplastic agents ; Biological and medical sciences ; Blotting, Western ; Carcinoma, Non-Small-Cell Lung - metabolism ; DPPA2 ; Enzyme-Linked Immunosorbent Assay ; Gene Expression ; Humans ; immune responses to cancer ; Immunohistochemistry ; lung cancer ; Lung Neoplasms - metabolism ; Medical sciences ; Nuclear Proteins - biosynthesis ; Pharmacology. Drug treatments ; Pneumology ; Reverse Transcriptase Polymerase Chain Reaction ; tumor antigens ; Tumors of the respiratory system and mediastinum</subject><ispartof>Clinical cancer research, 2008-06, Vol.14 (11), p.3291-3298</ispartof><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c470t-a9676c8ab8a1e1fd62e3d0fda8ebea2af46b27004a892899fb96bc6b2a0eece33</citedby><cites>FETCH-LOGICAL-c470t-a9676c8ab8a1e1fd62e3d0fda8ebea2af46b27004a892899fb96bc6b2a0eece33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3343,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20491115$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18519755$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>JOHN, Thomas</creatorcontrib><creatorcontrib>CABALLERO, Otavia L</creatorcontrib><creatorcontrib>DAVIS, Lan D</creatorcontrib><creatorcontrib>ALTORKI, Nasser</creatorcontrib><creatorcontrib>SIMPSON, Andrew J</creatorcontrib><creatorcontrib>CHEN, Yao-Tseng</creatorcontrib><creatorcontrib>MONK, Marilyn</creatorcontrib><creatorcontrib>CEBON, Jonathan S</creatorcontrib><creatorcontrib>SVOBODOVA, Suzanne J</creatorcontrib><creatorcontrib>KONG, Alan</creatorcontrib><creatorcontrib>CHUA, Ramon</creatorcontrib><creatorcontrib>BROWNING, Judy</creatorcontrib><creatorcontrib>FORTUNATO, Sheila</creatorcontrib><creatorcontrib>DEB, Siddhartha</creatorcontrib><creatorcontrib>HSU, Melinda</creatorcontrib><creatorcontrib>GEDYE, Craig A</creatorcontrib><title>ECSA/DPPA2 is an Embryo-Cancer Antigen that Is Coexpressed with Cancer-Testis Antigens in Non–Small Cell Lung Cancer</title><title>Clinical cancer research</title><addtitle>Clin Cancer Res</addtitle><description>Purpose: Cancer cells recapitulate many behaviors of pluripotent embryonic cells such as unlimited proliferation, and the capacity
to self-renew and to migrate. Embryo-cancer sequence A ( ECSA ), later named developmental pluripotency associated-2 ( DPPA2 ), is an embryonic gene initially isolated from pluripotent human preimplantation embryos. We hypothesized that ECSA/DPPA2
would be quiescent in most normal tissues but expressed in cancers and may therefore be a useful target for immunotherapy.
Experimental Design: ECSA/DPPA 2 expression was examined in a panel of normal and tumor tissue by reverse transcription PCR, quantitative real-time PCR,
and immunohistochemistry. A panel of 110 non–small cell lung cancers (NSCLC) were further investigated for the presence of
ECSA/DPPA2 transcripts and several cancer testis antigens (CTA). Sera from 104 patients were analyzed for spontaneous ECSA/DPPA2 antibody production by ELISA and Western blot.
Results: ECSA/DPPA 2 transcripts were limited to normal testis, placenta, bone marrow, thymus, and kidney but expressed in a variety of tumors
most notably in 30% of NSCLC. Enrichment for CTAs in ECSA/DPPA2 -positive NSCLC was observed. Immunohistochemistry confirmed nuclear and cytoplasmic localization in subpopulations of cells
with coexpression of the CTA MAGE-A3. Antibodies to recombinant ECSA/DPPA2 protein were detected in the sera of 4 of 104 patients
with NSCLC but not in healthy controls.
Conclusions: The restricted expression in normal tissues, expression in tumors with coexpression of CTAs, and spontaneous immunogenicity
indicate that ECSA/DPPA 2 is a promising target for antigen-specific immunotherapy in NSCLC.</description><subject>Antigens, Neoplasm - biosynthesis</subject><subject>Antineoplastic agents</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Carcinoma, Non-Small-Cell Lung - metabolism</subject><subject>DPPA2</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>immune responses to cancer</subject><subject>Immunohistochemistry</subject><subject>lung cancer</subject><subject>Lung Neoplasms - metabolism</subject><subject>Medical sciences</subject><subject>Nuclear Proteins - biosynthesis</subject><subject>Pharmacology. Drug treatments</subject><subject>Pneumology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>tumor antigens</subject><subject>Tumors of the respiratory system and mediastinum</subject><issn>1078-0432</issn><issn>1557-3265</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkMFO3DAURa2qVaGUTwB500osDLbjxMlylA4FaVRQgbX14rxMXE2Swc6Usus_9A_5EjxMChvbss59vj6EHAl-KkSanwmuc8ZVIk_L8ifjmolEyndkX6SpZonM0vfx_J_ZI59C-MW5UIKrj2RP5KkodJruk9_z8mZ29u36eiapCxR6Ou8q_ziwEnqLns760S2xp2MLI70MtBzwz9pjCFjTBze2dMexWwxjzE94oK6nP4b-6e-_mw5WK1piXBabfjnxn8mHBlYBD6f9gNydz2_LC7a4-n5ZzhbMKs1HBkWmM5tDlYNA0dSZxKTmTQ05VggSGpVVUnOuIC9kXhRNVWSVjXfAES0myQH5upu79sP9JnY0nQs2loEeh00wWmRScZ5FMN2B1g8heGzM2rsO_KMR3GyFm61Ms5VponDDtdkKj7nj6YFN1WH9lpoMR-DLBECwsGp8_L8Lr5zkqhBxfuROdlzrlu2D82jsi6noGsHb1ggVe5hEFiJ5BpXil9Y</recordid><startdate>20080601</startdate><enddate>20080601</enddate><creator>JOHN, Thomas</creator><creator>CABALLERO, Otavia L</creator><creator>DAVIS, Lan D</creator><creator>ALTORKI, Nasser</creator><creator>SIMPSON, Andrew J</creator><creator>CHEN, Yao-Tseng</creator><creator>MONK, Marilyn</creator><creator>CEBON, Jonathan S</creator><creator>SVOBODOVA, Suzanne J</creator><creator>KONG, Alan</creator><creator>CHUA, Ramon</creator><creator>BROWNING, Judy</creator><creator>FORTUNATO, Sheila</creator><creator>DEB, Siddhartha</creator><creator>HSU, Melinda</creator><creator>GEDYE, Craig A</creator><general>American Association for Cancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080601</creationdate><title>ECSA/DPPA2 is an Embryo-Cancer Antigen that Is Coexpressed with Cancer-Testis Antigens in Non–Small Cell Lung Cancer</title><author>JOHN, Thomas ; CABALLERO, Otavia L ; DAVIS, Lan D ; ALTORKI, Nasser ; SIMPSON, Andrew J ; CHEN, Yao-Tseng ; MONK, Marilyn ; CEBON, Jonathan S ; SVOBODOVA, Suzanne J ; KONG, Alan ; CHUA, Ramon ; BROWNING, Judy ; FORTUNATO, Sheila ; DEB, Siddhartha ; HSU, Melinda ; GEDYE, Craig A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c470t-a9676c8ab8a1e1fd62e3d0fda8ebea2af46b27004a892899fb96bc6b2a0eece33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Antigens, Neoplasm - biosynthesis</topic><topic>Antineoplastic agents</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Carcinoma, Non-Small-Cell Lung - metabolism</topic><topic>DPPA2</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Gene Expression</topic><topic>Humans</topic><topic>immune responses to cancer</topic><topic>Immunohistochemistry</topic><topic>lung cancer</topic><topic>Lung Neoplasms - metabolism</topic><topic>Medical sciences</topic><topic>Nuclear Proteins - biosynthesis</topic><topic>Pharmacology. Drug treatments</topic><topic>Pneumology</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>tumor antigens</topic><topic>Tumors of the respiratory system and mediastinum</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>JOHN, Thomas</creatorcontrib><creatorcontrib>CABALLERO, Otavia L</creatorcontrib><creatorcontrib>DAVIS, Lan D</creatorcontrib><creatorcontrib>ALTORKI, Nasser</creatorcontrib><creatorcontrib>SIMPSON, Andrew J</creatorcontrib><creatorcontrib>CHEN, Yao-Tseng</creatorcontrib><creatorcontrib>MONK, Marilyn</creatorcontrib><creatorcontrib>CEBON, Jonathan S</creatorcontrib><creatorcontrib>SVOBODOVA, Suzanne J</creatorcontrib><creatorcontrib>KONG, Alan</creatorcontrib><creatorcontrib>CHUA, Ramon</creatorcontrib><creatorcontrib>BROWNING, Judy</creatorcontrib><creatorcontrib>FORTUNATO, Sheila</creatorcontrib><creatorcontrib>DEB, Siddhartha</creatorcontrib><creatorcontrib>HSU, Melinda</creatorcontrib><creatorcontrib>GEDYE, Craig A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical cancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>JOHN, Thomas</au><au>CABALLERO, Otavia L</au><au>DAVIS, Lan D</au><au>ALTORKI, Nasser</au><au>SIMPSON, Andrew J</au><au>CHEN, Yao-Tseng</au><au>MONK, Marilyn</au><au>CEBON, Jonathan S</au><au>SVOBODOVA, Suzanne J</au><au>KONG, Alan</au><au>CHUA, Ramon</au><au>BROWNING, Judy</au><au>FORTUNATO, Sheila</au><au>DEB, Siddhartha</au><au>HSU, Melinda</au><au>GEDYE, Craig A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ECSA/DPPA2 is an Embryo-Cancer Antigen that Is Coexpressed with Cancer-Testis Antigens in Non–Small Cell Lung Cancer</atitle><jtitle>Clinical cancer research</jtitle><addtitle>Clin Cancer Res</addtitle><date>2008-06-01</date><risdate>2008</risdate><volume>14</volume><issue>11</issue><spage>3291</spage><epage>3298</epage><pages>3291-3298</pages><issn>1078-0432</issn><eissn>1557-3265</eissn><abstract>Purpose: Cancer cells recapitulate many behaviors of pluripotent embryonic cells such as unlimited proliferation, and the capacity
to self-renew and to migrate. Embryo-cancer sequence A ( ECSA ), later named developmental pluripotency associated-2 ( DPPA2 ), is an embryonic gene initially isolated from pluripotent human preimplantation embryos. We hypothesized that ECSA/DPPA2
would be quiescent in most normal tissues but expressed in cancers and may therefore be a useful target for immunotherapy.
Experimental Design: ECSA/DPPA 2 expression was examined in a panel of normal and tumor tissue by reverse transcription PCR, quantitative real-time PCR,
and immunohistochemistry. A panel of 110 non–small cell lung cancers (NSCLC) were further investigated for the presence of
ECSA/DPPA2 transcripts and several cancer testis antigens (CTA). Sera from 104 patients were analyzed for spontaneous ECSA/DPPA2 antibody production by ELISA and Western blot.
Results: ECSA/DPPA 2 transcripts were limited to normal testis, placenta, bone marrow, thymus, and kidney but expressed in a variety of tumors
most notably in 30% of NSCLC. Enrichment for CTAs in ECSA/DPPA2 -positive NSCLC was observed. Immunohistochemistry confirmed nuclear and cytoplasmic localization in subpopulations of cells
with coexpression of the CTA MAGE-A3. Antibodies to recombinant ECSA/DPPA2 protein were detected in the sera of 4 of 104 patients
with NSCLC but not in healthy controls.
Conclusions: The restricted expression in normal tissues, expression in tumors with coexpression of CTAs, and spontaneous immunogenicity
indicate that ECSA/DPPA 2 is a promising target for antigen-specific immunotherapy in NSCLC.</abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>18519755</pmid><doi>10.1158/1078-0432.CCR-07-1322</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Clinical cancer research, 2008-06, Vol.14 (11), p.3291-3298 |
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| source | MEDLINE; American Association for Cancer Research; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Antigens, Neoplasm - biosynthesis Antineoplastic agents Biological and medical sciences Blotting, Western Carcinoma, Non-Small-Cell Lung - metabolism DPPA2 Enzyme-Linked Immunosorbent Assay Gene Expression Humans immune responses to cancer Immunohistochemistry lung cancer Lung Neoplasms - metabolism Medical sciences Nuclear Proteins - biosynthesis Pharmacology. Drug treatments Pneumology Reverse Transcriptase Polymerase Chain Reaction tumor antigens Tumors of the respiratory system and mediastinum |
| title | ECSA/DPPA2 is an Embryo-Cancer Antigen that Is Coexpressed with Cancer-Testis Antigens in Non–Small Cell Lung Cancer |
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