Hydrophilic interaction liquid chromatography method for the determination of ascorbic acid
Hydrophilic interaction liquid chromatography (HILIC) method using internal standard for the determination and stability study of ascorbic acid was developed. HILIC method was very fast and simple using the following analytical conditions: ZIC HILIC (150x2.1 mm, 3.5 μm) chromatographic column and mo...
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| Veröffentlicht in: | Journal of separation science 2008-05, Vol.31 (9), p.1634-1644 |
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| container_title | Journal of separation science |
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| creator | Nováková, Lucie Solichová, Dagmar Pavlovičová, Soňa Solich, Petr |
| description | Hydrophilic interaction liquid chromatography (HILIC) method using internal standard for the determination and stability study of ascorbic acid was developed. HILIC method was very fast and simple using the following analytical conditions: ZIC HILIC (150x2.1 mm, 3.5 μm) chromatographic column and mobile phase composed of ACN and 50 mM ammonium acetate buffer pH 6.8 (78:22 v/v). Diode array detection was performed and chromatograms were processed at 268 nm, the maximum wavelength of absorbance of ascorbic acid. An extensive stability study of ascorbic acid as a function of various factors including temperature, stabilizing agents, oxygen presence and its concentration in solution was performed in order to gain information about the quantitative influence of individual stability factors. Low temperature and stabilizing agents (o-phosphoric acid and oxalic acid) were found to be key factors enabling substantial enhancement of the stability of ascorbic acid. |
| doi_str_mv | 10.1002/jssc.200700570 |
| format | Article |
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HILIC method was very fast and simple using the following analytical conditions: ZIC HILIC (150x2.1 mm, 3.5 μm) chromatographic column and mobile phase composed of ACN and 50 mM ammonium acetate buffer pH 6.8 (78:22 v/v). Diode array detection was performed and chromatograms were processed at 268 nm, the maximum wavelength of absorbance of ascorbic acid. An extensive stability study of ascorbic acid as a function of various factors including temperature, stabilizing agents, oxygen presence and its concentration in solution was performed in order to gain information about the quantitative influence of individual stability factors. Low temperature and stabilizing agents (o-phosphoric acid and oxalic acid) were found to be key factors enabling substantial enhancement of the stability of ascorbic acid.</description><identifier>ISSN: 1615-9306</identifier><identifier>EISSN: 1615-9314</identifier><identifier>DOI: 10.1002/jssc.200700570</identifier><identifier>PMID: 18428189</identifier><language>eng</language><publisher>Weinheim: Wiley-VCH Verlag</publisher><subject>Ascorbic acid ; Ascorbic Acid - analysis ; Ascorbic Acid - metabolism ; Ascorbic Acid - standards ; Chlorogenic Acid - analysis ; Chlorogenic Acid - standards ; Chromatography, High Pressure Liquid - methods ; Chromatography, Liquid - methods ; Chromatography, Liquid - standards ; Drug Stability ; HILIC ; Humans ; Hydrogen-Ion Concentration ; Indicators and Reagents ; o-phosphoric acid ; Oxalic acid ; Oxygen ; Phase Transition ; Reference Standards ; Solvents ; Spectrophotometry, Ultraviolet ; Stability ; Temperature ; Vitamins</subject><ispartof>Journal of separation science, 2008-05, Vol.31 (9), p.1634-1644</ispartof><rights>Copyright © 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3660-df12e51322265b4297b30396a12ae05ad923ba91be1467098303515bfcb8ba0c3</citedby><cites>FETCH-LOGICAL-c3660-df12e51322265b4297b30396a12ae05ad923ba91be1467098303515bfcb8ba0c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjssc.200700570$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjssc.200700570$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18428189$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nováková, Lucie</creatorcontrib><creatorcontrib>Solichová, Dagmar</creatorcontrib><creatorcontrib>Pavlovičová, Soňa</creatorcontrib><creatorcontrib>Solich, Petr</creatorcontrib><title>Hydrophilic interaction liquid chromatography method for the determination of ascorbic acid</title><title>Journal of separation science</title><addtitle>J. Sep. Science</addtitle><description>Hydrophilic interaction liquid chromatography (HILIC) method using internal standard for the determination and stability study of ascorbic acid was developed. HILIC method was very fast and simple using the following analytical conditions: ZIC HILIC (150x2.1 mm, 3.5 μm) chromatographic column and mobile phase composed of ACN and 50 mM ammonium acetate buffer pH 6.8 (78:22 v/v). Diode array detection was performed and chromatograms were processed at 268 nm, the maximum wavelength of absorbance of ascorbic acid. An extensive stability study of ascorbic acid as a function of various factors including temperature, stabilizing agents, oxygen presence and its concentration in solution was performed in order to gain information about the quantitative influence of individual stability factors. Low temperature and stabilizing agents (o-phosphoric acid and oxalic acid) were found to be key factors enabling substantial enhancement of the stability of ascorbic acid.</description><subject>Ascorbic acid</subject><subject>Ascorbic Acid - analysis</subject><subject>Ascorbic Acid - metabolism</subject><subject>Ascorbic Acid - standards</subject><subject>Chlorogenic Acid - analysis</subject><subject>Chlorogenic Acid - standards</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Chromatography, Liquid - methods</subject><subject>Chromatography, Liquid - standards</subject><subject>Drug Stability</subject><subject>HILIC</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Indicators and Reagents</subject><subject>o-phosphoric acid</subject><subject>Oxalic acid</subject><subject>Oxygen</subject><subject>Phase Transition</subject><subject>Reference Standards</subject><subject>Solvents</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>Stability</subject><subject>Temperature</subject><subject>Vitamins</subject><issn>1615-9306</issn><issn>1615-9314</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1v0zAYhy0EYmNw5Qg5cUt5X7vxxxEq1oE6kCgTBw6W7TiLR1J3dqrR_56MVGW3nfxKfp7n8CPkNcIMAej7m5zdjAIIgErAE3KKHKtSMZw_Pd7AT8iLnG8AUEgFz8kJyjmVKNUp-XWxr1PctqELrgibwSfjhhA3RRdud6EuXJtib4Z4ncy23Re9H9pYF01MxdD6ovaj0IeN-afEpjDZxWTHlHGhfkmeNabL_tXhPSNX559-LC7K1bfl58WHVekY51DWDVJfIaOU8srOqRKWAVPcIDUeKlMryqxRaD3OuQAlx98KK9s4K60Bx87Iu6m7TfF25_Og-5Cd7zqz8XGXtUBOgXP6KMgYA0k5juBsAl2KOSff6G0KvUl7jaDvd9f3u-vj7qPw5lDe2d7X__HD0COgJuAudH7_SE5_Wa8XD-Pl5IY8-D9H16TfmgsmKv3z61J_VMvV5UKi_j7ybye-MVGb6xSyvlpTQAaggAkJ7C_tR6fq</recordid><startdate>200805</startdate><enddate>200805</enddate><creator>Nováková, Lucie</creator><creator>Solichová, Dagmar</creator><creator>Pavlovičová, Soňa</creator><creator>Solich, Petr</creator><general>Wiley-VCH Verlag</general><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>200805</creationdate><title>Hydrophilic interaction liquid chromatography method for the determination of ascorbic acid</title><author>Nováková, Lucie ; Solichová, Dagmar ; Pavlovičová, Soňa ; Solich, Petr</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3660-df12e51322265b4297b30396a12ae05ad923ba91be1467098303515bfcb8ba0c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Ascorbic acid</topic><topic>Ascorbic Acid - analysis</topic><topic>Ascorbic Acid - metabolism</topic><topic>Ascorbic Acid - standards</topic><topic>Chlorogenic Acid - analysis</topic><topic>Chlorogenic Acid - standards</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Chromatography, Liquid - methods</topic><topic>Chromatography, Liquid - standards</topic><topic>Drug Stability</topic><topic>HILIC</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Indicators and Reagents</topic><topic>o-phosphoric acid</topic><topic>Oxalic acid</topic><topic>Oxygen</topic><topic>Phase Transition</topic><topic>Reference Standards</topic><topic>Solvents</topic><topic>Spectrophotometry, Ultraviolet</topic><topic>Stability</topic><topic>Temperature</topic><topic>Vitamins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nováková, Lucie</creatorcontrib><creatorcontrib>Solichová, Dagmar</creatorcontrib><creatorcontrib>Pavlovičová, Soňa</creatorcontrib><creatorcontrib>Solich, Petr</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of separation science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nováková, Lucie</au><au>Solichová, Dagmar</au><au>Pavlovičová, Soňa</au><au>Solich, Petr</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hydrophilic interaction liquid chromatography method for the determination of ascorbic acid</atitle><jtitle>Journal of separation science</jtitle><addtitle>J. Sep. Science</addtitle><date>2008-05</date><risdate>2008</risdate><volume>31</volume><issue>9</issue><spage>1634</spage><epage>1644</epage><pages>1634-1644</pages><issn>1615-9306</issn><eissn>1615-9314</eissn><abstract>Hydrophilic interaction liquid chromatography (HILIC) method using internal standard for the determination and stability study of ascorbic acid was developed. HILIC method was very fast and simple using the following analytical conditions: ZIC HILIC (150x2.1 mm, 3.5 μm) chromatographic column and mobile phase composed of ACN and 50 mM ammonium acetate buffer pH 6.8 (78:22 v/v). Diode array detection was performed and chromatograms were processed at 268 nm, the maximum wavelength of absorbance of ascorbic acid. An extensive stability study of ascorbic acid as a function of various factors including temperature, stabilizing agents, oxygen presence and its concentration in solution was performed in order to gain information about the quantitative influence of individual stability factors. Low temperature and stabilizing agents (o-phosphoric acid and oxalic acid) were found to be key factors enabling substantial enhancement of the stability of ascorbic acid.</abstract><cop>Weinheim</cop><pub>Wiley-VCH Verlag</pub><pmid>18428189</pmid><doi>10.1002/jssc.200700570</doi><tpages>11</tpages></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Ascorbic acid Ascorbic Acid - analysis Ascorbic Acid - metabolism Ascorbic Acid - standards Chlorogenic Acid - analysis Chlorogenic Acid - standards Chromatography, High Pressure Liquid - methods Chromatography, Liquid - methods Chromatography, Liquid - standards Drug Stability HILIC Humans Hydrogen-Ion Concentration Indicators and Reagents o-phosphoric acid Oxalic acid Oxygen Phase Transition Reference Standards Solvents Spectrophotometry, Ultraviolet Stability Temperature Vitamins |
| title | Hydrophilic interaction liquid chromatography method for the determination of ascorbic acid |
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