Leishmania mexicana: Identification of Genes That Are Preferentially Expressed in Amastigotes
Bellatin, J. A., Murray, A. S., Zhao, M., and McMaster, W. R. 2001. Leishmania mexicana: Identification of genes that are preferentially expressed in amastigotes. Experimental Parasitology100, 44–53. The protozoan parasite Leishmania has a digenetic life cycle, alternating between the promastigote a...
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| description | Bellatin, J. A., Murray, A. S., Zhao, M., and McMaster, W. R. 2001. Leishmania mexicana: Identification of genes that are preferentially expressed in amastigotes. Experimental Parasitology100, 44–53. The protozoan parasite Leishmania has a digenetic life cycle, alternating between the promastigote and the amastigote stages. Amastigotes infect macrophage cells and reside in the hydrolytic environment of the phagolysosome. Leishmania show distinct morphological and biochemical changes during differentiation into amastigotes. These alterations are believed to be regulated by stage-specific expression of a discrete number of genes. Selective-suppression PCR, a PCR-based subtractive hybridization technique, identified two genes preferentially expressed in L. mexicana lesion amastigotes: a novel gene family, A600, and a differentially expressed β-tubulin gene. Northern blot analysis confirmed amastigote-specific expression of these genes and quantitation showed a sixfold higher abundance of A600 and β-tubulin transcripts in lesion amastigotes. The A600 gene was predicted to contain a 293-bp open reading frame (ORF) that was tandemly repeated in the L. mexicana genome. Sequence analysis predicted that the A600 ORF encodes either a membrane-bound or a secreted protein that may have a functional role in amastigote differentiation or intraphagolysosomal parasite survival. |
| doi_str_mv | 10.1006/expr.2001.4677 |
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A., Murray, A. S., Zhao, M., and McMaster, W. R. 2001. Leishmania mexicana: Identification of genes that are preferentially expressed in amastigotes. Experimental Parasitology100, 44–53. The protozoan parasite Leishmania has a digenetic life cycle, alternating between the promastigote and the amastigote stages. Amastigotes infect macrophage cells and reside in the hydrolytic environment of the phagolysosome. Leishmania show distinct morphological and biochemical changes during differentiation into amastigotes. These alterations are believed to be regulated by stage-specific expression of a discrete number of genes. Selective-suppression PCR, a PCR-based subtractive hybridization technique, identified two genes preferentially expressed in L. mexicana lesion amastigotes: a novel gene family, A600, and a differentially expressed β-tubulin gene. Northern blot analysis confirmed amastigote-specific expression of these genes and quantitation showed a sixfold higher abundance of A600 and β-tubulin transcripts in lesion amastigotes. The A600 gene was predicted to contain a 293-bp open reading frame (ORF) that was tandemly repeated in the L. mexicana genome. 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Psychology ; gene expression ; Gene Expression Regulation ; Genome, Protozoan ; Leishmania mexicana ; Leishmania mexicana - chemistry ; Leishmania mexicana - genetics ; Life cycle. Host-agent relationship. 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A., Murray, A. S., Zhao, M., and McMaster, W. R. 2001. Leishmania mexicana: Identification of genes that are preferentially expressed in amastigotes. Experimental Parasitology100, 44–53. The protozoan parasite Leishmania has a digenetic life cycle, alternating between the promastigote and the amastigote stages. Amastigotes infect macrophage cells and reside in the hydrolytic environment of the phagolysosome. Leishmania show distinct morphological and biochemical changes during differentiation into amastigotes. These alterations are believed to be regulated by stage-specific expression of a discrete number of genes. Selective-suppression PCR, a PCR-based subtractive hybridization technique, identified two genes preferentially expressed in L. mexicana lesion amastigotes: a novel gene family, A600, and a differentially expressed β-tubulin gene. Northern blot analysis confirmed amastigote-specific expression of these genes and quantitation showed a sixfold higher abundance of A600 and β-tubulin transcripts in lesion amastigotes. The A600 gene was predicted to contain a 293-bp open reading frame (ORF) that was tandemly repeated in the L. mexicana genome. Sequence analysis predicted that the A600 ORF encodes either a membrane-bound or a secreted protein that may have a functional role in amastigote differentiation or intraphagolysosomal parasite survival.</description><subject>A600 gene</subject><subject>amastigote</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>b-tubulin</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Blotting, Southern</subject><subject>DNA, Complementary - analysis</subject><subject>DNA, Complementary - chemistry</subject><subject>DNA, Complementary - genetics</subject><subject>DNA, Protozoan - analysis</subject><subject>DNA, Protozoan - chemistry</subject><subject>DNA, Protozoan - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression</subject><subject>Gene Expression Regulation</subject><subject>Genome, Protozoan</subject><subject>Leishmania mexicana</subject><subject>Leishmania mexicana - chemistry</subject><subject>Leishmania mexicana - genetics</subject><subject>Life cycle. Host-agent relationship. Pathogenesis</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>Open Reading Frames</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Protozoa</subject><subject>Protozoan Proteins - genetics</subject><subject>Protozoan Proteins - physiology</subject><subject>Restriction Mapping</subject><subject>RNA, Protozoan - genetics</subject><subject>Sequence Alignment</subject><subject>Species Specificity</subject><subject>Tubulin - chemistry</subject><subject>Tubulin - genetics</subject><issn>0014-4894</issn><issn>1090-2449</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEFr3DAQRkVJaTZprz0WXdKbtyNZtuzeliVNAgvtIT0WIcujRsWWtxpvSf59ZXYhp5DTMMObj5nH2EcBawFQf8HHfVpLALFWtdZv2EpAC4VUqj1jqzxWhWpadc4uiP4AQCOkesfOhWi1qKtyxX7tMNDDaGOwfMTH4Gy0X_ldj3EOPndzmCKfPL_BiMTvH-zMNwn5j4Qe0wLZYXji1_kKJMKeh8g3o6U5_J5mpPfsrbcD4YdTvWQ_v13fb2-L3febu-1mVzgFYi4QBYpOQweVq5xH6BQo5aVuWtmhrZWoRelV553stexqbLStWl91zvXStVhess_H3H2a_h6QZjMGcjgMNuJ0IJOfXRxVr4KiKUVZap3B9RF0aSLK35p9CqNNT0aAWcybxbxZUs1iPi98OiUfuhH7Z_ykOgNXJ8CSs4NPNrpAz1xZ6xakylxz5DAL-xcwGXIBo8M-JHSz6afw0g3_ARmCoG8</recordid><startdate>200201</startdate><enddate>200201</enddate><creator>Bellatin, J.A.</creator><creator>Murray, A.S.</creator><creator>Zhao, M.</creator><creator>McMaster, W.R.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200201</creationdate><title>Leishmania mexicana: Identification of Genes That Are Preferentially Expressed in Amastigotes</title><author>Bellatin, J.A. ; Murray, A.S. ; Zhao, M. ; McMaster, W.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c401t-ee1e1b70b05c5cfe0b4044f27892bea641613f4bfc2d72b6e87a59f5bccd2c9e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>A600 gene</topic><topic>amastigote</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>b-tubulin</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Blotting, Southern</topic><topic>DNA, Complementary - analysis</topic><topic>DNA, Complementary - chemistry</topic><topic>DNA, Complementary - genetics</topic><topic>DNA, Protozoan - analysis</topic><topic>DNA, Protozoan - chemistry</topic><topic>DNA, Protozoan - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression</topic><topic>Gene Expression Regulation</topic><topic>Genome, Protozoan</topic><topic>Leishmania mexicana</topic><topic>Leishmania mexicana - chemistry</topic><topic>Leishmania mexicana - genetics</topic><topic>Life cycle. Host-agent relationship. Pathogenesis</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Multigene Family</topic><topic>Open Reading Frames</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Protozoa</topic><topic>Protozoan Proteins - genetics</topic><topic>Protozoan Proteins - physiology</topic><topic>Restriction Mapping</topic><topic>RNA, Protozoan - genetics</topic><topic>Sequence Alignment</topic><topic>Species Specificity</topic><topic>Tubulin - chemistry</topic><topic>Tubulin - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bellatin, J.A.</creatorcontrib><creatorcontrib>Murray, A.S.</creatorcontrib><creatorcontrib>Zhao, M.</creatorcontrib><creatorcontrib>McMaster, W.R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bellatin, J.A.</au><au>Murray, A.S.</au><au>Zhao, M.</au><au>McMaster, W.R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Leishmania mexicana: Identification of Genes That Are Preferentially Expressed in Amastigotes</atitle><jtitle>Experimental parasitology</jtitle><addtitle>Exp Parasitol</addtitle><date>2002-01</date><risdate>2002</risdate><volume>100</volume><issue>1</issue><spage>44</spage><epage>53</epage><pages>44-53</pages><issn>0014-4894</issn><eissn>1090-2449</eissn><coden>EXPAAA</coden><abstract>Bellatin, J. A., Murray, A. S., Zhao, M., and McMaster, W. R. 2001. Leishmania mexicana: Identification of genes that are preferentially expressed in amastigotes. Experimental Parasitology100, 44–53. The protozoan parasite Leishmania has a digenetic life cycle, alternating between the promastigote and the amastigote stages. Amastigotes infect macrophage cells and reside in the hydrolytic environment of the phagolysosome. Leishmania show distinct morphological and biochemical changes during differentiation into amastigotes. These alterations are believed to be regulated by stage-specific expression of a discrete number of genes. Selective-suppression PCR, a PCR-based subtractive hybridization technique, identified two genes preferentially expressed in L. mexicana lesion amastigotes: a novel gene family, A600, and a differentially expressed β-tubulin gene. Northern blot analysis confirmed amastigote-specific expression of these genes and quantitation showed a sixfold higher abundance of A600 and β-tubulin transcripts in lesion amastigotes. The A600 gene was predicted to contain a 293-bp open reading frame (ORF) that was tandemly repeated in the L. mexicana genome. Sequence analysis predicted that the A600 ORF encodes either a membrane-bound or a secreted protein that may have a functional role in amastigote differentiation or intraphagolysosomal parasite survival.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>11971653</pmid><doi>10.1006/expr.2001.4677</doi><tpages>10</tpages></addata></record> |
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| subjects | A600 gene amastigote Amino Acid Sequence Animals b-tubulin Biological and medical sciences Blotting, Northern Blotting, Southern DNA, Complementary - analysis DNA, Complementary - chemistry DNA, Complementary - genetics DNA, Protozoan - analysis DNA, Protozoan - chemistry DNA, Protozoan - genetics Fundamental and applied biological sciences. Psychology gene expression Gene Expression Regulation Genome, Protozoan Leishmania mexicana Leishmania mexicana - chemistry Leishmania mexicana - genetics Life cycle. Host-agent relationship. Pathogenesis Mice Molecular Sequence Data Multigene Family Open Reading Frames Polymerase Chain Reaction - methods Protozoa Protozoan Proteins - genetics Protozoan Proteins - physiology Restriction Mapping RNA, Protozoan - genetics Sequence Alignment Species Specificity Tubulin - chemistry Tubulin - genetics |
| title | Leishmania mexicana: Identification of Genes That Are Preferentially Expressed in Amastigotes |
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