Novel autoantigens recognized by CSF IgG from Hashimoto's encephalitis revealed by a proteomic approach
Abstract To identify the target of IgG autoimmune response in Hashimoto's encephalopathy (HE), we studied the binding of IgG present in serum and cerebro-spinal fluid (CSF) from six patients with HE and 15 controls to human central nervous system (CNS) white matter antigens by 2D-PAGE and immun...
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| Veröffentlicht in: | Journal of neuroimmunology 2008-05, Vol.196 (1), p.153-158 |
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| creator | Gini, Beatrice Laura, Lovato Riccardo, Cianti Laura, Cecotti Marconi, Silvia Anghileri, Elena Alessandro, Armini Giuseppe, Moretto Luca, Bini Franco, Ferracci Bruno, Bonetti |
| description | Abstract To identify the target of IgG autoimmune response in Hashimoto's encephalopathy (HE), we studied the binding of IgG present in serum and cerebro-spinal fluid (CSF) from six patients with HE and 15 controls to human central nervous system (CNS) white matter antigens by 2D-PAGE and immunoblotting and by immunohistochemistry. We found that CSF IgG from HE patients specifically recognized 3 spots, which were identified as dimethylargininase-I (DDAHI) and aldehyde reductase-I (AKRIAI). DDAHI was present in two isoforms recognized respectively by five and four HE patients; immunohistochemistry with anti-DDAHI antiserum depicted endothelial cells in normal human CNS. AKRIAI was recognized by three HE CSF and this enzyme was widely distributed on neurons and endothelia by immunohistochemistry. IgG from HE CSF immunostained both neuronal and endothelial cells in mouse CNS. The presence of these autoantibodies selectively in the CSF of HE patients may have important diagnostic and pathogenetic implications, since the autoimmune response to these enzymes may lead to vascular and/or neuronal damage, two major mechanisms involved in the pathogenesis of HE. |
| doi_str_mv | 10.1016/j.jneuroim.2008.02.015 |
| format | Article |
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We found that CSF IgG from HE patients specifically recognized 3 spots, which were identified as dimethylargininase-I (DDAHI) and aldehyde reductase-I (AKRIAI). DDAHI was present in two isoforms recognized respectively by five and four HE patients; immunohistochemistry with anti-DDAHI antiserum depicted endothelial cells in normal human CNS. AKRIAI was recognized by three HE CSF and this enzyme was widely distributed on neurons and endothelia by immunohistochemistry. IgG from HE CSF immunostained both neuronal and endothelial cells in mouse CNS. The presence of these autoantibodies selectively in the CSF of HE patients may have important diagnostic and pathogenetic implications, since the autoimmune response to these enzymes may lead to vascular and/or neuronal damage, two major mechanisms involved in the pathogenesis of HE.</description><identifier>ISSN: 0165-5728</identifier><identifier>EISSN: 1872-8421</identifier><identifier>DOI: 10.1016/j.jneuroim.2008.02.015</identifier><identifier>PMID: 18407358</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Aged ; AKRIAI ; Aldehyde Reductase - metabolism ; Allergy and Immunology ; Amidohydrolases - metabolism ; Autoantibodies ; Autoantigens - cerebrospinal fluid ; Autoantigens - immunology ; Blood Vessels - metabolism ; DDAHI ; Electrophoresis, Gel, Two-Dimensional - methods ; Female ; Hashimoto Disease - cerebrospinal fluid ; Hashimoto Disease - immunology ; Hashimoto Disease - pathology ; Hashimoto's encephalitis ; Humans ; Immunoglobulin G - cerebrospinal fluid ; Male ; Mass Spectrometry - methods ; Middle Aged ; Neurology ; Proteomics ; Proteomics - methods</subject><ispartof>Journal of neuroimmunology, 2008-05, Vol.196 (1), p.153-158</ispartof><rights>Elsevier B.V.</rights><rights>2008 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c518t-5d73f7aed0e7476d2a85c455fb1d66c629c22d1818797af8b94c852fd5e470383</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jneuroim.2008.02.015$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18407358$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gini, Beatrice</creatorcontrib><creatorcontrib>Laura, Lovato</creatorcontrib><creatorcontrib>Riccardo, Cianti</creatorcontrib><creatorcontrib>Laura, Cecotti</creatorcontrib><creatorcontrib>Marconi, Silvia</creatorcontrib><creatorcontrib>Anghileri, Elena</creatorcontrib><creatorcontrib>Alessandro, Armini</creatorcontrib><creatorcontrib>Giuseppe, Moretto</creatorcontrib><creatorcontrib>Luca, Bini</creatorcontrib><creatorcontrib>Franco, Ferracci</creatorcontrib><creatorcontrib>Bruno, Bonetti</creatorcontrib><title>Novel autoantigens recognized by CSF IgG from Hashimoto's encephalitis revealed by a proteomic approach</title><title>Journal of neuroimmunology</title><addtitle>J Neuroimmunol</addtitle><description>Abstract To identify the target of IgG autoimmune response in Hashimoto's encephalopathy (HE), we studied the binding of IgG present in serum and cerebro-spinal fluid (CSF) from six patients with HE and 15 controls to human central nervous system (CNS) white matter antigens by 2D-PAGE and immunoblotting and by immunohistochemistry. We found that CSF IgG from HE patients specifically recognized 3 spots, which were identified as dimethylargininase-I (DDAHI) and aldehyde reductase-I (AKRIAI). DDAHI was present in two isoforms recognized respectively by five and four HE patients; immunohistochemistry with anti-DDAHI antiserum depicted endothelial cells in normal human CNS. AKRIAI was recognized by three HE CSF and this enzyme was widely distributed on neurons and endothelia by immunohistochemistry. IgG from HE CSF immunostained both neuronal and endothelial cells in mouse CNS. The presence of these autoantibodies selectively in the CSF of HE patients may have important diagnostic and pathogenetic implications, since the autoimmune response to these enzymes may lead to vascular and/or neuronal damage, two major mechanisms involved in the pathogenesis of HE.</description><subject>Aged</subject><subject>AKRIAI</subject><subject>Aldehyde Reductase - metabolism</subject><subject>Allergy and Immunology</subject><subject>Amidohydrolases - metabolism</subject><subject>Autoantibodies</subject><subject>Autoantigens - cerebrospinal fluid</subject><subject>Autoantigens - immunology</subject><subject>Blood Vessels - metabolism</subject><subject>DDAHI</subject><subject>Electrophoresis, Gel, Two-Dimensional - methods</subject><subject>Female</subject><subject>Hashimoto Disease - cerebrospinal fluid</subject><subject>Hashimoto Disease - immunology</subject><subject>Hashimoto Disease - pathology</subject><subject>Hashimoto's encephalitis</subject><subject>Humans</subject><subject>Immunoglobulin G - cerebrospinal fluid</subject><subject>Male</subject><subject>Mass Spectrometry - methods</subject><subject>Middle Aged</subject><subject>Neurology</subject><subject>Proteomics</subject><subject>Proteomics - methods</subject><issn>0165-5728</issn><issn>1872-8421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks2O0zAUhS0EYsrAK4y8glWC7dixs0GgivmRRrAYWFuufdM6JHGxk0rl6XGmHSGxmZXv4jv3yOdchK4oKSmh9ceu7EaYY_BDyQhRJWEloeIFWlElWaE4oy_RKoOiEJKpC_QmpY5kouLNa3RBFSeyEmqFtt_CAXps5imYcfJbGBOOYMN29H_A4c0Rrx-u8d32BrcxDPjWpJ0fwhQ-JAyjhf3O9H7yi-YApj8pDN7HMEEYvMVmn2djd2_Rq9b0Cd6d30v08_rrj_Vtcf_95m795b6wgqqpEE5WrTTgCEgua8eMEpYL0W6oq2tbs8Yy5qjKv2ykadWm4VYJ1joBXJJKVZfo_Wlvtv09Q5r04JOFvjcjhDlpSWuqVM7hOZA2XBEu6gzWJ9DGkFKEVu-jH0w8akr00oXu9FMXeulCE6bJo8PV2WHeDOD-yc7hZ-DzCYAcyMFD1Mn6JVXncwWTdsE_7_HpvxW296O3pv8FR0hdmOOY49ZUpyzQD8tFLAdBFCFEKFr9BSEZs50</recordid><startdate>20080530</startdate><enddate>20080530</enddate><creator>Gini, Beatrice</creator><creator>Laura, Lovato</creator><creator>Riccardo, Cianti</creator><creator>Laura, Cecotti</creator><creator>Marconi, Silvia</creator><creator>Anghileri, Elena</creator><creator>Alessandro, Armini</creator><creator>Giuseppe, Moretto</creator><creator>Luca, Bini</creator><creator>Franco, Ferracci</creator><creator>Bruno, Bonetti</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TK</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20080530</creationdate><title>Novel autoantigens recognized by CSF IgG from Hashimoto's encephalitis revealed by a proteomic approach</title><author>Gini, Beatrice ; Laura, Lovato ; Riccardo, Cianti ; Laura, Cecotti ; Marconi, Silvia ; Anghileri, Elena ; Alessandro, Armini ; Giuseppe, Moretto ; Luca, Bini ; Franco, Ferracci ; Bruno, Bonetti</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c518t-5d73f7aed0e7476d2a85c455fb1d66c629c22d1818797af8b94c852fd5e470383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Aged</topic><topic>AKRIAI</topic><topic>Aldehyde Reductase - metabolism</topic><topic>Allergy and Immunology</topic><topic>Amidohydrolases - metabolism</topic><topic>Autoantibodies</topic><topic>Autoantigens - cerebrospinal fluid</topic><topic>Autoantigens - immunology</topic><topic>Blood Vessels - metabolism</topic><topic>DDAHI</topic><topic>Electrophoresis, Gel, Two-Dimensional - methods</topic><topic>Female</topic><topic>Hashimoto Disease - cerebrospinal fluid</topic><topic>Hashimoto Disease - immunology</topic><topic>Hashimoto Disease - pathology</topic><topic>Hashimoto's encephalitis</topic><topic>Humans</topic><topic>Immunoglobulin G - cerebrospinal fluid</topic><topic>Male</topic><topic>Mass Spectrometry - methods</topic><topic>Middle Aged</topic><topic>Neurology</topic><topic>Proteomics</topic><topic>Proteomics - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gini, Beatrice</creatorcontrib><creatorcontrib>Laura, Lovato</creatorcontrib><creatorcontrib>Riccardo, Cianti</creatorcontrib><creatorcontrib>Laura, Cecotti</creatorcontrib><creatorcontrib>Marconi, Silvia</creatorcontrib><creatorcontrib>Anghileri, Elena</creatorcontrib><creatorcontrib>Alessandro, Armini</creatorcontrib><creatorcontrib>Giuseppe, Moretto</creatorcontrib><creatorcontrib>Luca, Bini</creatorcontrib><creatorcontrib>Franco, Ferracci</creatorcontrib><creatorcontrib>Bruno, Bonetti</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuroimmunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gini, Beatrice</au><au>Laura, Lovato</au><au>Riccardo, Cianti</au><au>Laura, Cecotti</au><au>Marconi, Silvia</au><au>Anghileri, Elena</au><au>Alessandro, Armini</au><au>Giuseppe, Moretto</au><au>Luca, Bini</au><au>Franco, Ferracci</au><au>Bruno, Bonetti</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Novel autoantigens recognized by CSF IgG from Hashimoto's encephalitis revealed by a proteomic approach</atitle><jtitle>Journal of neuroimmunology</jtitle><addtitle>J Neuroimmunol</addtitle><date>2008-05-30</date><risdate>2008</risdate><volume>196</volume><issue>1</issue><spage>153</spage><epage>158</epage><pages>153-158</pages><issn>0165-5728</issn><eissn>1872-8421</eissn><abstract>Abstract To identify the target of IgG autoimmune response in Hashimoto's encephalopathy (HE), we studied the binding of IgG present in serum and cerebro-spinal fluid (CSF) from six patients with HE and 15 controls to human central nervous system (CNS) white matter antigens by 2D-PAGE and immunoblotting and by immunohistochemistry. We found that CSF IgG from HE patients specifically recognized 3 spots, which were identified as dimethylargininase-I (DDAHI) and aldehyde reductase-I (AKRIAI). DDAHI was present in two isoforms recognized respectively by five and four HE patients; immunohistochemistry with anti-DDAHI antiserum depicted endothelial cells in normal human CNS. AKRIAI was recognized by three HE CSF and this enzyme was widely distributed on neurons and endothelia by immunohistochemistry. IgG from HE CSF immunostained both neuronal and endothelial cells in mouse CNS. The presence of these autoantibodies selectively in the CSF of HE patients may have important diagnostic and pathogenetic implications, since the autoimmune response to these enzymes may lead to vascular and/or neuronal damage, two major mechanisms involved in the pathogenesis of HE.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>18407358</pmid><doi>10.1016/j.jneuroim.2008.02.015</doi><tpages>6</tpages></addata></record> |
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| subjects | Aged AKRIAI Aldehyde Reductase - metabolism Allergy and Immunology Amidohydrolases - metabolism Autoantibodies Autoantigens - cerebrospinal fluid Autoantigens - immunology Blood Vessels - metabolism DDAHI Electrophoresis, Gel, Two-Dimensional - methods Female Hashimoto Disease - cerebrospinal fluid Hashimoto Disease - immunology Hashimoto Disease - pathology Hashimoto's encephalitis Humans Immunoglobulin G - cerebrospinal fluid Male Mass Spectrometry - methods Middle Aged Neurology Proteomics Proteomics - methods |
| title | Novel autoantigens recognized by CSF IgG from Hashimoto's encephalitis revealed by a proteomic approach |
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