Cell Type-specific Expression of the Mas Proto-oncogene in Testis
The Mas proto-oncogene encodes a G-protein-coupled receptor with the common seven transmembrane domains and may be involved in the actions of angiotensins. Because Mas is highly expressed in testis, we investigated the cell type-specificity and the onset of expression of the gene in this organ. Usin...
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| Veröffentlicht in: | The journal of histochemistry and cytochemistry 2002-05, Vol.50 (5), p.691-695 |
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| container_end_page | 695 |
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| container_issue | 5 |
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| container_title | The journal of histochemistry and cytochemistry |
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| creator | Alenina, Natalia Baranova, Tatjana Smirnow, Eugene Bader, Michael Lippoldt, Andrea Patkin, Eugene Walther, Thomas |
| description | The Mas proto-oncogene encodes a G-protein-coupled receptor with the common seven transmembrane domains and may be involved in the actions of angiotensins. Because Mas is highly expressed in testis, we investigated the cell type-specificity and the onset of expression of the gene in this organ. Using an RNase protection assay, it could be shown that neither whole testes nor cultured Sertoli and Leydig cells of 12-day-old mice express Mas mRNA. Mas expression is first detected in 18-day-old mice and thereafter increases continuously until 6 months of age. By in situ hybridization, the expression could be localized to Leydig cells and Sertoli cells, the signals being much more pronounced in the former. A weak signal was detected in primary spermatocytes. The strong ontogenetically controlled and cell type-specific expression of this membrane-bound receptor in testis implicates a role for the Mas proto-oncogene in testis maturation and function. |
| doi_str_mv | 10.1177/002215540205000510 |
| format | Article |
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Because Mas is highly expressed in testis, we investigated the cell type-specificity and the onset of expression of the gene in this organ. Using an RNase protection assay, it could be shown that neither whole testes nor cultured Sertoli and Leydig cells of 12-day-old mice express Mas mRNA. Mas expression is first detected in 18-day-old mice and thereafter increases continuously until 6 months of age. By in situ hybridization, the expression could be localized to Leydig cells and Sertoli cells, the signals being much more pronounced in the former. A weak signal was detected in primary spermatocytes. The strong ontogenetically controlled and cell type-specific expression of this membrane-bound receptor in testis implicates a role for the Mas proto-oncogene in testis maturation and function.</description><identifier>ISSN: 0022-1554</identifier><identifier>EISSN: 1551-5044</identifier><identifier>DOI: 10.1177/002215540205000510</identifier><identifier>PMID: 11967280</identifier><language>eng</language><publisher>Los Angeles, CA: Histochemical Soc</publisher><subject>Animals ; Cell Line ; In Situ Hybridization, Fluorescence ; Leydig Cells - metabolism ; Male ; Mice ; Mice, Inbred Strains ; Mice, Knockout ; Proto-Oncogene Proteins - metabolism ; Receptors, G-Protein-Coupled ; Ribonucleases ; RNA, Messenger - metabolism ; Sertoli Cells - metabolism ; Testis - cytology ; Testis - metabolism</subject><ispartof>The journal of histochemistry and cytochemistry, 2002-05, Vol.50 (5), p.691-695</ispartof><rights>2002 Authors</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c369t-9b377797d88674288611b756454b8fc9956cd681402a0da1dfb4cae076d001383</citedby><cites>FETCH-LOGICAL-c369t-9b377797d88674288611b756454b8fc9956cd681402a0da1dfb4cae076d001383</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/002215540205000510$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/002215540205000510$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,776,780,21798,27901,27902,43597,43598</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11967280$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alenina, Natalia</creatorcontrib><creatorcontrib>Baranova, Tatjana</creatorcontrib><creatorcontrib>Smirnow, Eugene</creatorcontrib><creatorcontrib>Bader, Michael</creatorcontrib><creatorcontrib>Lippoldt, Andrea</creatorcontrib><creatorcontrib>Patkin, Eugene</creatorcontrib><creatorcontrib>Walther, Thomas</creatorcontrib><title>Cell Type-specific Expression of the Mas Proto-oncogene in Testis</title><title>The journal of histochemistry and cytochemistry</title><addtitle>J Histochem Cytochem</addtitle><description>The Mas proto-oncogene encodes a G-protein-coupled receptor with the common seven transmembrane domains and may be involved in the actions of angiotensins. Because Mas is highly expressed in testis, we investigated the cell type-specificity and the onset of expression of the gene in this organ. Using an RNase protection assay, it could be shown that neither whole testes nor cultured Sertoli and Leydig cells of 12-day-old mice express Mas mRNA. Mas expression is first detected in 18-day-old mice and thereafter increases continuously until 6 months of age. By in situ hybridization, the expression could be localized to Leydig cells and Sertoli cells, the signals being much more pronounced in the former. A weak signal was detected in primary spermatocytes. The strong ontogenetically controlled and cell type-specific expression of this membrane-bound receptor in testis implicates a role for the Mas proto-oncogene in testis maturation and function.</description><subject>Animals</subject><subject>Cell Line</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Leydig Cells - metabolism</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>Mice, Knockout</subject><subject>Proto-Oncogene Proteins - metabolism</subject><subject>Receptors, G-Protein-Coupled</subject><subject>Ribonucleases</subject><subject>RNA, Messenger - metabolism</subject><subject>Sertoli Cells - metabolism</subject><subject>Testis - cytology</subject><subject>Testis - metabolism</subject><issn>0022-1554</issn><issn>1551-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtOwzAQRS0EouXxAyxQNrALnUn8SJZVxUsqgkVZW47jtK7SONitSv8eV63EAonNjDQ6987MJeQG4QFRiBFAliFjFDJgAMAQTsgwDjBlQOkpGe6BdE8MyEUISwCklBXnZIBYcpEVMCTjiWnbZLbrTRp6o21jdfL43XsTgnVd4ppkvTDJmwrJh3drl7pOu7npTGK7ZGbC2oYrctaoNpjrY78kn0-Ps8lLOn1_fp2Mp6nOeblOyyoXQpSiLgouaBYrYiUYp4xWRaPLknFd8wLjNwpqhXVTUa0MCF7Hu_MivyT3B9_eu69NXC1XNuh4veqM2wQpkGORCRHB7ABq70LwppG9tyvldxJB7oOTf4OLotuj-6ZamfpXckwqAqMDENTcyKXb-C5--7_l3UGxsPPF1nojw0q1bVyAcrvdMpBM8hLzHxjJgBs</recordid><startdate>20020501</startdate><enddate>20020501</enddate><creator>Alenina, Natalia</creator><creator>Baranova, Tatjana</creator><creator>Smirnow, Eugene</creator><creator>Bader, Michael</creator><creator>Lippoldt, Andrea</creator><creator>Patkin, Eugene</creator><creator>Walther, Thomas</creator><general>Histochemical Soc</general><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020501</creationdate><title>Cell Type-specific Expression of the Mas Proto-oncogene in Testis</title><author>Alenina, Natalia ; Baranova, Tatjana ; Smirnow, Eugene ; Bader, Michael ; Lippoldt, Andrea ; Patkin, Eugene ; Walther, Thomas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c369t-9b377797d88674288611b756454b8fc9956cd681402a0da1dfb4cae076d001383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Cell Line</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Leydig Cells - metabolism</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>Mice, Knockout</topic><topic>Proto-Oncogene Proteins - metabolism</topic><topic>Receptors, G-Protein-Coupled</topic><topic>Ribonucleases</topic><topic>RNA, Messenger - metabolism</topic><topic>Sertoli Cells - metabolism</topic><topic>Testis - cytology</topic><topic>Testis - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alenina, Natalia</creatorcontrib><creatorcontrib>Baranova, Tatjana</creatorcontrib><creatorcontrib>Smirnow, Eugene</creatorcontrib><creatorcontrib>Bader, Michael</creatorcontrib><creatorcontrib>Lippoldt, Andrea</creatorcontrib><creatorcontrib>Patkin, Eugene</creatorcontrib><creatorcontrib>Walther, Thomas</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The journal of histochemistry and cytochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alenina, Natalia</au><au>Baranova, Tatjana</au><au>Smirnow, Eugene</au><au>Bader, Michael</au><au>Lippoldt, Andrea</au><au>Patkin, Eugene</au><au>Walther, Thomas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cell Type-specific Expression of the Mas Proto-oncogene in Testis</atitle><jtitle>The journal of histochemistry and cytochemistry</jtitle><addtitle>J Histochem Cytochem</addtitle><date>2002-05-01</date><risdate>2002</risdate><volume>50</volume><issue>5</issue><spage>691</spage><epage>695</epage><pages>691-695</pages><issn>0022-1554</issn><eissn>1551-5044</eissn><abstract>The Mas proto-oncogene encodes a G-protein-coupled receptor with the common seven transmembrane domains and may be involved in the actions of angiotensins. 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| subjects | Animals Cell Line In Situ Hybridization, Fluorescence Leydig Cells - metabolism Male Mice Mice, Inbred Strains Mice, Knockout Proto-Oncogene Proteins - metabolism Receptors, G-Protein-Coupled Ribonucleases RNA, Messenger - metabolism Sertoli Cells - metabolism Testis - cytology Testis - metabolism |
| title | Cell Type-specific Expression of the Mas Proto-oncogene in Testis |
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