Expression of Nitrotyrosine and Oxidative Consequences in the Trabecular Meshwork of Patients with Primary Open-Angle Glaucoma
To evaluate the possible correlation between the visual field defects in patients with primary open-angle glaucoma (POAG) and the expression and enzymatic activity of nitric oxide synthase (NOS) isoenzymes and nitrotyrosine in trabecular meshwork (TM) samples. TM specimens were collected from 146 pa...
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| Veröffentlicht in: | Investigative ophthalmology & visual science 2008-06, Vol.49 (6), p.2506-2511 |
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| creator | Fernandez-Durango, Raquel Fernandez-Martinez, Amalia Garcia-Feijoo, Julian Castillo, Alfredo de la Casa, Jose Martinez Garcia-Bueno, Borja Perez-Nievas, Beatriz G Fernandez-Cruz, Arturo Leza, Juan Carlos |
| description | To evaluate the possible correlation between the visual field defects in patients with primary open-angle glaucoma (POAG) and the expression and enzymatic activity of nitric oxide synthase (NOS) isoenzymes and nitrotyrosine in trabecular meshwork (TM) samples.
TM specimens were collected from 146 patients with POAG by using standard filtration surgery. Visual field defects were evaluated by perimetry. Expression of endothelial (e)NOS and inducible (i)NOS were evaluated by quantitative RT-PCR. Constitutive (Ca2+-dependent) and iNOS (Ca2+-independent) activities were measured by the conversion of L-[14C]-arginine to L-[14C]-citrulline. In four TM specimens from POAG-affected eyes and in three human donor control eyes, 3-nitrotyrosine was localized by immunohistochemistry. The marker of lipid peroxidation malondialdehyde (MDA) was measured by the thiobarbituric acid test in samples of aqueous humor (AH) from 48 patients with either POAG or cataracts.
The results showed an upregulation of iNOS and a downregulation of calcium-dependent NOS correlated with visual field defects. Expression and activity of iNOS increased in parallel with visual field defects. However, constitutive activity decreased as the visual field defect increased. Nitrotyrosine was observed only in the cells of the TM specimens from eyes with severe POAG.
The increased expression and activity of iNOS in the TM of patients with POAG are proportional to the visual field defect and could lead to the increased of nitrotyrosine levels which may serve as marker of oxidative stress in the progression of cell death of the TM in POAG. |
| doi_str_mv | 10.1167/iovs.07-1363 |
| format | Article |
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TM specimens were collected from 146 patients with POAG by using standard filtration surgery. Visual field defects were evaluated by perimetry. Expression of endothelial (e)NOS and inducible (i)NOS were evaluated by quantitative RT-PCR. Constitutive (Ca2+-dependent) and iNOS (Ca2+-independent) activities were measured by the conversion of L-[14C]-arginine to L-[14C]-citrulline. In four TM specimens from POAG-affected eyes and in three human donor control eyes, 3-nitrotyrosine was localized by immunohistochemistry. The marker of lipid peroxidation malondialdehyde (MDA) was measured by the thiobarbituric acid test in samples of aqueous humor (AH) from 48 patients with either POAG or cataracts.
The results showed an upregulation of iNOS and a downregulation of calcium-dependent NOS correlated with visual field defects. Expression and activity of iNOS increased in parallel with visual field defects. However, constitutive activity decreased as the visual field defect increased. Nitrotyrosine was observed only in the cells of the TM specimens from eyes with severe POAG.
The increased expression and activity of iNOS in the TM of patients with POAG are proportional to the visual field defect and could lead to the increased of nitrotyrosine levels which may serve as marker of oxidative stress in the progression of cell death of the TM in POAG.</description><identifier>ISSN: 0146-0404</identifier><identifier>ISSN: 1552-5783</identifier><identifier>EISSN: 1552-5783</identifier><identifier>DOI: 10.1167/iovs.07-1363</identifier><identifier>PMID: 18296660</identifier><identifier>CODEN: IOVSDA</identifier><language>eng</language><publisher>Rockville, MD: ARVO</publisher><subject>Aged ; Aged, 80 and over ; Biological and medical sciences ; Calcium - pharmacology ; Eye and associated structures. Visual pathways and centers. Vision ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Enzymologic - physiology ; Glaucoma and intraocular pressure ; Glaucoma, Open-Angle - metabolism ; Humans ; Intraocular Pressure ; Lipid Peroxidation ; Malondialdehyde - metabolism ; Medical sciences ; Middle Aged ; Nitric Oxide Synthase Type II - genetics ; Nitric Oxide Synthase Type III - genetics ; Ophthalmology ; Oxidative Stress - physiology ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - metabolism ; Trabecular Meshwork - metabolism ; Tyrosine - analogs & derivatives ; Tyrosine - metabolism ; Up-Regulation ; Vertebrates: nervous system and sense organs ; Vision Disorders - metabolism ; Visual Field Tests ; Visual Fields</subject><ispartof>Investigative ophthalmology & visual science, 2008-06, Vol.49 (6), p.2506-2511</ispartof><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c350t-cc37b1638ada31d49603d30a8f30c8f4a247edb47f8763966885ad8248ab4e253</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20406069$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18296660$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fernandez-Durango, Raquel</creatorcontrib><creatorcontrib>Fernandez-Martinez, Amalia</creatorcontrib><creatorcontrib>Garcia-Feijoo, Julian</creatorcontrib><creatorcontrib>Castillo, Alfredo</creatorcontrib><creatorcontrib>de la Casa, Jose Martinez</creatorcontrib><creatorcontrib>Garcia-Bueno, Borja</creatorcontrib><creatorcontrib>Perez-Nievas, Beatriz G</creatorcontrib><creatorcontrib>Fernandez-Cruz, Arturo</creatorcontrib><creatorcontrib>Leza, Juan Carlos</creatorcontrib><title>Expression of Nitrotyrosine and Oxidative Consequences in the Trabecular Meshwork of Patients with Primary Open-Angle Glaucoma</title><title>Investigative ophthalmology & visual science</title><addtitle>Invest Ophthalmol Vis Sci</addtitle><description>To evaluate the possible correlation between the visual field defects in patients with primary open-angle glaucoma (POAG) and the expression and enzymatic activity of nitric oxide synthase (NOS) isoenzymes and nitrotyrosine in trabecular meshwork (TM) samples.
TM specimens were collected from 146 patients with POAG by using standard filtration surgery. Visual field defects were evaluated by perimetry. Expression of endothelial (e)NOS and inducible (i)NOS were evaluated by quantitative RT-PCR. Constitutive (Ca2+-dependent) and iNOS (Ca2+-independent) activities were measured by the conversion of L-[14C]-arginine to L-[14C]-citrulline. In four TM specimens from POAG-affected eyes and in three human donor control eyes, 3-nitrotyrosine was localized by immunohistochemistry. The marker of lipid peroxidation malondialdehyde (MDA) was measured by the thiobarbituric acid test in samples of aqueous humor (AH) from 48 patients with either POAG or cataracts.
The results showed an upregulation of iNOS and a downregulation of calcium-dependent NOS correlated with visual field defects. Expression and activity of iNOS increased in parallel with visual field defects. However, constitutive activity decreased as the visual field defect increased. Nitrotyrosine was observed only in the cells of the TM specimens from eyes with severe POAG.
The increased expression and activity of iNOS in the TM of patients with POAG are proportional to the visual field defect and could lead to the increased of nitrotyrosine levels which may serve as marker of oxidative stress in the progression of cell death of the TM in POAG.</description><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biological and medical sciences</subject><subject>Calcium - pharmacology</subject><subject>Eye and associated structures. Visual pathways and centers. Vision</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Enzymologic - physiology</subject><subject>Glaucoma and intraocular pressure</subject><subject>Glaucoma, Open-Angle - metabolism</subject><subject>Humans</subject><subject>Intraocular Pressure</subject><subject>Lipid Peroxidation</subject><subject>Malondialdehyde - metabolism</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Nitric Oxide Synthase Type II - genetics</subject><subject>Nitric Oxide Synthase Type III - genetics</subject><subject>Ophthalmology</subject><subject>Oxidative Stress - physiology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>Trabecular Meshwork - metabolism</subject><subject>Tyrosine - analogs & derivatives</subject><subject>Tyrosine - metabolism</subject><subject>Up-Regulation</subject><subject>Vertebrates: nervous system and sense organs</subject><subject>Vision Disorders - metabolism</subject><subject>Visual Field Tests</subject><subject>Visual Fields</subject><issn>0146-0404</issn><issn>1552-5783</issn><issn>1552-5783</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkU1v1DAQhi0Eokvhxhn5Qk-k-CuO91itSlupsD2UszVxJo0hsRc7adoLv52sdkVPc3nm1TvPEPKRs3POdfXVx8d8zqqCSy1fkRUvS1GUlZGvyYpxpQummDoh73L-xZjgXLC35IQbsdZasxX5e_m0S5izj4HGlv7wY4rjc4rZB6QQGrp98g2M_hHpJoaMfyYMDjP1gY4d0vsENbqph0S_Y-7mmH7vY-6WDQxjprMfO3qX_ADpmW53GIqL8NAjvephcnGA9-RNC33GD8d5Sn5-u7zfXBe326ubzcVt4WTJxsI5WdVcSwMNSN6otWaykQxMK5kzrQKhKmxqVbWm0nI5zZgSGiOUgVqhKOUpOTvk7lJcTsijHXx22PcQME7ZVlxzXXK-gF8OoFsc5ISt3R3aW87s3rfd-7assnvfC_7pmDvVAzYv8FHwAnw-ApAd9G2C4Hz-z4nlO5rp9UvBzj90s09o8wB9v8RyO8-zWlttRcm0_Aeaa5ft</recordid><startdate>20080601</startdate><enddate>20080601</enddate><creator>Fernandez-Durango, Raquel</creator><creator>Fernandez-Martinez, Amalia</creator><creator>Garcia-Feijoo, Julian</creator><creator>Castillo, Alfredo</creator><creator>de la Casa, Jose Martinez</creator><creator>Garcia-Bueno, Borja</creator><creator>Perez-Nievas, Beatriz G</creator><creator>Fernandez-Cruz, Arturo</creator><creator>Leza, Juan Carlos</creator><general>ARVO</general><general>Association for Research in Vision and Ophtalmology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080601</creationdate><title>Expression of Nitrotyrosine and Oxidative Consequences in the Trabecular Meshwork of Patients with Primary Open-Angle Glaucoma</title><author>Fernandez-Durango, Raquel ; Fernandez-Martinez, Amalia ; Garcia-Feijoo, Julian ; Castillo, Alfredo ; de la Casa, Jose Martinez ; Garcia-Bueno, Borja ; Perez-Nievas, Beatriz G ; Fernandez-Cruz, Arturo ; Leza, Juan Carlos</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c350t-cc37b1638ada31d49603d30a8f30c8f4a247edb47f8763966885ad8248ab4e253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biological and medical sciences</topic><topic>Calcium - pharmacology</topic><topic>Eye and associated structures. Visual pathways and centers. Vision</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Enzymologic - physiology</topic><topic>Glaucoma and intraocular pressure</topic><topic>Glaucoma, Open-Angle - metabolism</topic><topic>Humans</topic><topic>Intraocular Pressure</topic><topic>Lipid Peroxidation</topic><topic>Malondialdehyde - metabolism</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Nitric Oxide Synthase Type II - genetics</topic><topic>Nitric Oxide Synthase Type III - genetics</topic><topic>Ophthalmology</topic><topic>Oxidative Stress - physiology</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>Trabecular Meshwork - metabolism</topic><topic>Tyrosine - analogs & derivatives</topic><topic>Tyrosine - metabolism</topic><topic>Up-Regulation</topic><topic>Vertebrates: nervous system and sense organs</topic><topic>Vision Disorders - metabolism</topic><topic>Visual Field Tests</topic><topic>Visual Fields</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fernandez-Durango, Raquel</creatorcontrib><creatorcontrib>Fernandez-Martinez, Amalia</creatorcontrib><creatorcontrib>Garcia-Feijoo, Julian</creatorcontrib><creatorcontrib>Castillo, Alfredo</creatorcontrib><creatorcontrib>de la Casa, Jose Martinez</creatorcontrib><creatorcontrib>Garcia-Bueno, Borja</creatorcontrib><creatorcontrib>Perez-Nievas, Beatriz G</creatorcontrib><creatorcontrib>Fernandez-Cruz, Arturo</creatorcontrib><creatorcontrib>Leza, Juan Carlos</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Investigative ophthalmology & visual science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fernandez-Durango, Raquel</au><au>Fernandez-Martinez, Amalia</au><au>Garcia-Feijoo, Julian</au><au>Castillo, Alfredo</au><au>de la Casa, Jose Martinez</au><au>Garcia-Bueno, Borja</au><au>Perez-Nievas, Beatriz G</au><au>Fernandez-Cruz, Arturo</au><au>Leza, Juan Carlos</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of Nitrotyrosine and Oxidative Consequences in the Trabecular Meshwork of Patients with Primary Open-Angle Glaucoma</atitle><jtitle>Investigative ophthalmology & visual science</jtitle><addtitle>Invest Ophthalmol Vis Sci</addtitle><date>2008-06-01</date><risdate>2008</risdate><volume>49</volume><issue>6</issue><spage>2506</spage><epage>2511</epage><pages>2506-2511</pages><issn>0146-0404</issn><issn>1552-5783</issn><eissn>1552-5783</eissn><coden>IOVSDA</coden><abstract>To evaluate the possible correlation between the visual field defects in patients with primary open-angle glaucoma (POAG) and the expression and enzymatic activity of nitric oxide synthase (NOS) isoenzymes and nitrotyrosine in trabecular meshwork (TM) samples.
TM specimens were collected from 146 patients with POAG by using standard filtration surgery. Visual field defects were evaluated by perimetry. Expression of endothelial (e)NOS and inducible (i)NOS were evaluated by quantitative RT-PCR. Constitutive (Ca2+-dependent) and iNOS (Ca2+-independent) activities were measured by the conversion of L-[14C]-arginine to L-[14C]-citrulline. In four TM specimens from POAG-affected eyes and in three human donor control eyes, 3-nitrotyrosine was localized by immunohistochemistry. The marker of lipid peroxidation malondialdehyde (MDA) was measured by the thiobarbituric acid test in samples of aqueous humor (AH) from 48 patients with either POAG or cataracts.
The results showed an upregulation of iNOS and a downregulation of calcium-dependent NOS correlated with visual field defects. Expression and activity of iNOS increased in parallel with visual field defects. However, constitutive activity decreased as the visual field defect increased. Nitrotyrosine was observed only in the cells of the TM specimens from eyes with severe POAG.
The increased expression and activity of iNOS in the TM of patients with POAG are proportional to the visual field defect and could lead to the increased of nitrotyrosine levels which may serve as marker of oxidative stress in the progression of cell death of the TM in POAG.</abstract><cop>Rockville, MD</cop><pub>ARVO</pub><pmid>18296660</pmid><doi>10.1167/iovs.07-1363</doi><tpages>6</tpages></addata></record> |
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| subjects | Aged Aged, 80 and over Biological and medical sciences Calcium - pharmacology Eye and associated structures. Visual pathways and centers. Vision Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Enzymologic - physiology Glaucoma and intraocular pressure Glaucoma, Open-Angle - metabolism Humans Intraocular Pressure Lipid Peroxidation Malondialdehyde - metabolism Medical sciences Middle Aged Nitric Oxide Synthase Type II - genetics Nitric Oxide Synthase Type III - genetics Ophthalmology Oxidative Stress - physiology Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Trabecular Meshwork - metabolism Tyrosine - analogs & derivatives Tyrosine - metabolism Up-Regulation Vertebrates: nervous system and sense organs Vision Disorders - metabolism Visual Field Tests Visual Fields |
| title | Expression of Nitrotyrosine and Oxidative Consequences in the Trabecular Meshwork of Patients with Primary Open-Angle Glaucoma |
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