Implantation-Associated Changes in Bovine Uterine Expression of Integrins and Extracellular Matrix
Appropriate integrin expression appears to be necessary for successful implantation of human embryos and varies considerably among species. The present study was undertaken to determine the distributions of integrin subunits α 1 , α 3 , and α 6 as well as the extracellular matrix (ECM) components...
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| Veröffentlicht in: | Biology of reproduction 2002-05, Vol.66 (5), p.1430-1436 |
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| container_title | Biology of reproduction |
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| creator | MACINTYRE, Doris M HAI CHOO LIM RYAN, Kim SARAH KIMMINS SMALL, Julie A MACLAREN, Leslie A |
| description | Appropriate integrin expression appears to be necessary for successful implantation of human embryos and varies considerably
among species. The present study was undertaken to determine the distributions of integrin subunits α 1 , α 3 , and α 6 as well as the extracellular matrix (ECM) components collagen IV and laminin in implanting bovine trophoblast and endometrium.
Immunohistochemical staining of cryostat sections prepared from nonpregnant endometrium, of preattachment through to early
villus development pregnant endometrium (Days 18, 21, 24, and 30), and of isolated trophoblast binucleate cells was performed.
Trophoblast down-regulated the integrin α 1 subunit as attachment proceeded, whereas reactivity scores for α 6 antibody tended to increase from Day 18 through 24 and remained high. A subpopulation of trophoblast binucleate cells expressed
the α 3 integrin subunit. Uterine epithelium constitutively expressed α 3 and α 6 integrin subunits, but the α 1 subunit was down-regulated as the luminal epithelium was modified. Collagen IV and laminin reactivity increased in the basal
lamina and underlying subepithelial stroma as pregnancy proceeded. The results suggest that binucleate cell fusion with the
maternal epithelium initiates integrin and ECM changes in the subepithelial stroma. |
| doi_str_mv | 10.1095/biolreprod66.5.1430 |
| format | Article |
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among species. The present study was undertaken to determine the distributions of integrin subunits α 1 , α 3 , and α 6 as well as the extracellular matrix (ECM) components collagen IV and laminin in implanting bovine trophoblast and endometrium.
Immunohistochemical staining of cryostat sections prepared from nonpregnant endometrium, of preattachment through to early
villus development pregnant endometrium (Days 18, 21, 24, and 30), and of isolated trophoblast binucleate cells was performed.
Trophoblast down-regulated the integrin α 1 subunit as attachment proceeded, whereas reactivity scores for α 6 antibody tended to increase from Day 18 through 24 and remained high. A subpopulation of trophoblast binucleate cells expressed
the α 3 integrin subunit. Uterine epithelium constitutively expressed α 3 and α 6 integrin subunits, but the α 1 subunit was down-regulated as the luminal epithelium was modified. Collagen IV and laminin reactivity increased in the basal
lamina and underlying subepithelial stroma as pregnancy proceeded. The results suggest that binucleate cell fusion with the
maternal epithelium initiates integrin and ECM changes in the subepithelial stroma.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod66.5.1430</identifier><identifier>PMID: 11967207</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Animals ; Biological and medical sciences ; Cattle ; Cell Nucleus - physiology ; Collagen Type IV - biosynthesis ; Embryo Implantation - physiology ; Embryology: invertebrates and vertebrates. Teratology ; Endometrium - metabolism ; Extracellular Matrix - metabolism ; Extracellular Matrix - ultrastructure ; Female ; Fetal membranes ; Fundamental and applied biological sciences. Psychology ; General aspects. Development. Fetal membranes ; Immunohistochemistry ; Integrins - biosynthesis ; Laminin - biosynthesis ; Pregnancy ; Trophoblasts - metabolism ; Ultrasonography ; Uterus - diagnostic imaging ; Uterus - metabolism</subject><ispartof>Biology of reproduction, 2002-05, Vol.66 (5), p.1430-1436</ispartof><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27915,27916</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13628869$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11967207$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MACINTYRE, Doris M</creatorcontrib><creatorcontrib>HAI CHOO LIM</creatorcontrib><creatorcontrib>RYAN, Kim</creatorcontrib><creatorcontrib>SARAH KIMMINS</creatorcontrib><creatorcontrib>SMALL, Julie A</creatorcontrib><creatorcontrib>MACLAREN, Leslie A</creatorcontrib><title>Implantation-Associated Changes in Bovine Uterine Expression of Integrins and Extracellular Matrix</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>Appropriate integrin expression appears to be necessary for successful implantation of human embryos and varies considerably
among species. The present study was undertaken to determine the distributions of integrin subunits α 1 , α 3 , and α 6 as well as the extracellular matrix (ECM) components collagen IV and laminin in implanting bovine trophoblast and endometrium.
Immunohistochemical staining of cryostat sections prepared from nonpregnant endometrium, of preattachment through to early
villus development pregnant endometrium (Days 18, 21, 24, and 30), and of isolated trophoblast binucleate cells was performed.
Trophoblast down-regulated the integrin α 1 subunit as attachment proceeded, whereas reactivity scores for α 6 antibody tended to increase from Day 18 through 24 and remained high. A subpopulation of trophoblast binucleate cells expressed
the α 3 integrin subunit. Uterine epithelium constitutively expressed α 3 and α 6 integrin subunits, but the α 1 subunit was down-regulated as the luminal epithelium was modified. Collagen IV and laminin reactivity increased in the basal
lamina and underlying subepithelial stroma as pregnancy proceeded. The results suggest that binucleate cell fusion with the
maternal epithelium initiates integrin and ECM changes in the subepithelial stroma.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Cell Nucleus - physiology</subject><subject>Collagen Type IV - biosynthesis</subject><subject>Embryo Implantation - physiology</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Endometrium - metabolism</subject><subject>Extracellular Matrix - metabolism</subject><subject>Extracellular Matrix - ultrastructure</subject><subject>Female</subject><subject>Fetal membranes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects. Development. Fetal membranes</subject><subject>Immunohistochemistry</subject><subject>Integrins - biosynthesis</subject><subject>Laminin - biosynthesis</subject><subject>Pregnancy</subject><subject>Trophoblasts - metabolism</subject><subject>Ultrasonography</subject><subject>Uterus - diagnostic imaging</subject><subject>Uterus - metabolism</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkU9P4zAQxS3ECkrhEyChXNhbuv7TTJojVCxUYrWX7TmaxJPWyHGK7dLy7dcVRXB60sxPb57eMHYt-ETwqvjVmMF62vhBA0yKiZgqfsJGopBVXkqYnbIR5xxypUCds4sQXjhPjFRn7FyICkrJyxFrFv3GoosYzeDyuxCG1mAknc3X6FYUMuOy--HNOMqWkfxBH_YbTyEkPhu6bOEirdI8ZOh02kWPLVm7teizPxi92V-yHx3aQFdHHbPl74d_86f8-e_jYn73nK9T2phDo7BCKJoUUQIRtZ1ArKakdQdSaqKmq9q2nE0FcEnTDrSCijrkWkvZVmrMfn74pkpetxRi3ZtwyIKOhm2oSwGiKPkBvDmC26YnXW-86dG_15-tJOD2CGBo0XYeXWvCF6dAzmbw7eLarNY746kOPVqbbFW92-0A6qI-vEX9BwH-gzA</recordid><startdate>20020501</startdate><enddate>20020501</enddate><creator>MACINTYRE, Doris M</creator><creator>HAI CHOO LIM</creator><creator>RYAN, Kim</creator><creator>SARAH KIMMINS</creator><creator>SMALL, Julie A</creator><creator>MACLAREN, Leslie A</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20020501</creationdate><title>Implantation-Associated Changes in Bovine Uterine Expression of Integrins and Extracellular Matrix</title><author>MACINTYRE, Doris M ; HAI CHOO LIM ; RYAN, Kim ; SARAH KIMMINS ; SMALL, Julie A ; MACLAREN, Leslie A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h268t-6b3a9a65b32326eeecf1aa94eddf622deebf9cc7841602e4f6d369efa0dd22c93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Cell Nucleus - physiology</topic><topic>Collagen Type IV - biosynthesis</topic><topic>Embryo Implantation - physiology</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Endometrium - metabolism</topic><topic>Extracellular Matrix - metabolism</topic><topic>Extracellular Matrix - ultrastructure</topic><topic>Female</topic><topic>Fetal membranes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects. Development. Fetal membranes</topic><topic>Immunohistochemistry</topic><topic>Integrins - biosynthesis</topic><topic>Laminin - biosynthesis</topic><topic>Pregnancy</topic><topic>Trophoblasts - metabolism</topic><topic>Ultrasonography</topic><topic>Uterus - diagnostic imaging</topic><topic>Uterus - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MACINTYRE, Doris M</creatorcontrib><creatorcontrib>HAI CHOO LIM</creatorcontrib><creatorcontrib>RYAN, Kim</creatorcontrib><creatorcontrib>SARAH KIMMINS</creatorcontrib><creatorcontrib>SMALL, Julie A</creatorcontrib><creatorcontrib>MACLAREN, Leslie A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MACINTYRE, Doris M</au><au>HAI CHOO LIM</au><au>RYAN, Kim</au><au>SARAH KIMMINS</au><au>SMALL, Julie A</au><au>MACLAREN, Leslie A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Implantation-Associated Changes in Bovine Uterine Expression of Integrins and Extracellular Matrix</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>2002-05-01</date><risdate>2002</risdate><volume>66</volume><issue>5</issue><spage>1430</spage><epage>1436</epage><pages>1430-1436</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>Appropriate integrin expression appears to be necessary for successful implantation of human embryos and varies considerably
among species. The present study was undertaken to determine the distributions of integrin subunits α 1 , α 3 , and α 6 as well as the extracellular matrix (ECM) components collagen IV and laminin in implanting bovine trophoblast and endometrium.
Immunohistochemical staining of cryostat sections prepared from nonpregnant endometrium, of preattachment through to early
villus development pregnant endometrium (Days 18, 21, 24, and 30), and of isolated trophoblast binucleate cells was performed.
Trophoblast down-regulated the integrin α 1 subunit as attachment proceeded, whereas reactivity scores for α 6 antibody tended to increase from Day 18 through 24 and remained high. A subpopulation of trophoblast binucleate cells expressed
the α 3 integrin subunit. Uterine epithelium constitutively expressed α 3 and α 6 integrin subunits, but the α 1 subunit was down-regulated as the luminal epithelium was modified. Collagen IV and laminin reactivity increased in the basal
lamina and underlying subepithelial stroma as pregnancy proceeded. The results suggest that binucleate cell fusion with the
maternal epithelium initiates integrin and ECM changes in the subepithelial stroma.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>11967207</pmid><doi>10.1095/biolreprod66.5.1430</doi><tpages>7</tpages></addata></record> |
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| source | MEDLINE; BioOne Complete; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Biological and medical sciences Cattle Cell Nucleus - physiology Collagen Type IV - biosynthesis Embryo Implantation - physiology Embryology: invertebrates and vertebrates. Teratology Endometrium - metabolism Extracellular Matrix - metabolism Extracellular Matrix - ultrastructure Female Fetal membranes Fundamental and applied biological sciences. Psychology General aspects. Development. Fetal membranes Immunohistochemistry Integrins - biosynthesis Laminin - biosynthesis Pregnancy Trophoblasts - metabolism Ultrasonography Uterus - diagnostic imaging Uterus - metabolism |
| title | Implantation-Associated Changes in Bovine Uterine Expression of Integrins and Extracellular Matrix |
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