Thrombin Generation and Presence of Thrombin Receptor in Ovarian Follicles
Prothrombin, once converted to its enzymatically active form (i.e., thrombin), induces a broad spectrum of cellular responses in both vascular and avascular tissues. Bovine ovarian granulosa cells isolated from healthy follicles of various sizes contain both prothrombin mRNA and immunologically reac...
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| Veröffentlicht in: | Biology of reproduction 2002-05, Vol.66 (5), p.1350-1358 |
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| container_title | Biology of reproduction |
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| creator | ROACH, Lindsay E PETRIK, James J PLANTE, Louise LAMARRE, Jonathan GENTRY, Patricia A |
| description | Prothrombin, once converted to its enzymatically active form (i.e., thrombin), induces a broad spectrum of cellular responses
in both vascular and avascular tissues. Bovine ovarian granulosa cells isolated from healthy follicles of various sizes contain
both prothrombin mRNA and immunologically reactive prothrombin that appears to be identical to prothrombin in follicular fluid
and plasma. When tissue factor, the primary physiological activator of thrombin generation in plasma, is used to initiate
thrombin formation, the profile of prothrombin-to-thrombin conversion is similar in follicular fluid and plasma. The conclusion
that biologically functional prothrombin is synthesized by granulosa cells is further supported by evidence that mRNA for
γ-glutamyl carboxylase, an enzyme essential for the vitamin K-dependent posttranslational modification of prothrombin, is
expressed in granulosa cells in a manner similar to prothrombin mRNA. Thrombin's biological effects are mediated through selective
proteolytic cleavage and activation of specific receptors. Bovine granulosa cells possess thrombin receptor (PAR-1) mRNA,
and as seen with prothrombin mRNA and γ-glutamyl carboxylase mRNA, cells isolated from small follicles possess more PAR-1
mRNA than cells from large follicles. Thrombin receptor expression by cells in close proximity to an active thrombin-generating
system suggests that these factors may be important mediators of cellular function in the ovarian follicle. |
| doi_str_mv | 10.1095/biolreprod66.5.1350 |
| format | Article |
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in both vascular and avascular tissues. Bovine ovarian granulosa cells isolated from healthy follicles of various sizes contain
both prothrombin mRNA and immunologically reactive prothrombin that appears to be identical to prothrombin in follicular fluid
and plasma. When tissue factor, the primary physiological activator of thrombin generation in plasma, is used to initiate
thrombin formation, the profile of prothrombin-to-thrombin conversion is similar in follicular fluid and plasma. The conclusion
that biologically functional prothrombin is synthesized by granulosa cells is further supported by evidence that mRNA for
γ-glutamyl carboxylase, an enzyme essential for the vitamin K-dependent posttranslational modification of prothrombin, is
expressed in granulosa cells in a manner similar to prothrombin mRNA. Thrombin's biological effects are mediated through selective
proteolytic cleavage and activation of specific receptors. Bovine granulosa cells possess thrombin receptor (PAR-1) mRNA,
and as seen with prothrombin mRNA and γ-glutamyl carboxylase mRNA, cells isolated from small follicles possess more PAR-1
mRNA than cells from large follicles. Thrombin receptor expression by cells in close proximity to an active thrombin-generating
system suggests that these factors may be important mediators of cellular function in the ovarian follicle.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod66.5.1350</identifier><identifier>PMID: 11967197</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Animals ; Biological and medical sciences ; Blotting, Northern ; Blotting, Western ; Carbon-Carbon Ligases - metabolism ; Cattle ; Cell Separation ; Electrophoresis, Polyacrylamide Gel ; Female ; Follicular Fluid - chemistry ; Fundamental and applied biological sciences. Psychology ; Granulosa Cells - metabolism ; Humans ; In Vitro Techniques ; Mammalian female genital system ; Morphology. Physiology ; Ovarian Follicle - metabolism ; Prothrombin - metabolism ; Receptors, Thrombin - biosynthesis ; Receptors, Thrombin - genetics ; Receptors, Thrombin - metabolism ; RNA, Messenger - biosynthesis ; Thrombin - biosynthesis ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 2002-05, Vol.66 (5), p.1350-1358</ispartof><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13628859$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11967197$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ROACH, Lindsay E</creatorcontrib><creatorcontrib>PETRIK, James J</creatorcontrib><creatorcontrib>PLANTE, Louise</creatorcontrib><creatorcontrib>LAMARRE, Jonathan</creatorcontrib><creatorcontrib>GENTRY, Patricia A</creatorcontrib><title>Thrombin Generation and Presence of Thrombin Receptor in Ovarian Follicles</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>Prothrombin, once converted to its enzymatically active form (i.e., thrombin), induces a broad spectrum of cellular responses
in both vascular and avascular tissues. Bovine ovarian granulosa cells isolated from healthy follicles of various sizes contain
both prothrombin mRNA and immunologically reactive prothrombin that appears to be identical to prothrombin in follicular fluid
and plasma. When tissue factor, the primary physiological activator of thrombin generation in plasma, is used to initiate
thrombin formation, the profile of prothrombin-to-thrombin conversion is similar in follicular fluid and plasma. The conclusion
that biologically functional prothrombin is synthesized by granulosa cells is further supported by evidence that mRNA for
γ-glutamyl carboxylase, an enzyme essential for the vitamin K-dependent posttranslational modification of prothrombin, is
expressed in granulosa cells in a manner similar to prothrombin mRNA. Thrombin's biological effects are mediated through selective
proteolytic cleavage and activation of specific receptors. Bovine granulosa cells possess thrombin receptor (PAR-1) mRNA,
and as seen with prothrombin mRNA and γ-glutamyl carboxylase mRNA, cells isolated from small follicles possess more PAR-1
mRNA than cells from large follicles. Thrombin receptor expression by cells in close proximity to an active thrombin-generating
system suggests that these factors may be important mediators of cellular function in the ovarian follicle.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Blotting, Western</subject><subject>Carbon-Carbon Ligases - metabolism</subject><subject>Cattle</subject><subject>Cell Separation</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Female</subject><subject>Follicular Fluid - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Granulosa Cells - metabolism</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Mammalian female genital system</subject><subject>Morphology. Physiology</subject><subject>Ovarian Follicle - metabolism</subject><subject>Prothrombin - metabolism</subject><subject>Receptors, Thrombin - biosynthesis</subject><subject>Receptors, Thrombin - genetics</subject><subject>Receptors, Thrombin - metabolism</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Thrombin - biosynthesis</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0E1PwzAMBuAIgdgY_AIk1AvcOvLRuO0RTWyAJg2hca7S1GVBaTOSjop_TxGDnezDI9uvCblkdMpoLm9L46zHrXcVwFROmZD0iIyZ5HmccsiOyZhSCrEQIEbkLIR3SlkiuDglI8ZySFmejsnTeuNdU5o2WmCLXnXGtZFqq-jZY8BWY-Tq6N-8oMZt53w09KtP5Y1qo7mz1miL4Zyc1MoGvNjXCXmd369nD_FytXic3S3jzXBVFyPNU5BYgtACtdYpr2qZpZnKuGIKeJKViQLQNecJLUsuWa04oEQJFZSSigm5-Z07RP_YYeiKxgSN1qoW3S4UKQMmkhQGeLWHu7LBqth60yj_VfylH8D1Hqigla29arUJByeAZ5nMDxs35m3TG49FaJS1w1hR9H0PUMji5_3iG_OIeJI</recordid><startdate>20020501</startdate><enddate>20020501</enddate><creator>ROACH, Lindsay E</creator><creator>PETRIK, James J</creator><creator>PLANTE, Louise</creator><creator>LAMARRE, Jonathan</creator><creator>GENTRY, Patricia A</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20020501</creationdate><title>Thrombin Generation and Presence of Thrombin Receptor in Ovarian Follicles</title><author>ROACH, Lindsay E ; PETRIK, James J ; PLANTE, Louise ; LAMARRE, Jonathan ; GENTRY, Patricia A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h268t-e09765eb63c3eccc72df5878a82a1a6248b4a66cf2240bb251fa26e5e56d6b503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Blotting, Western</topic><topic>Carbon-Carbon Ligases - metabolism</topic><topic>Cattle</topic><topic>Cell Separation</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Female</topic><topic>Follicular Fluid - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Granulosa Cells - metabolism</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Mammalian female genital system</topic><topic>Morphology. Physiology</topic><topic>Ovarian Follicle - metabolism</topic><topic>Prothrombin - metabolism</topic><topic>Receptors, Thrombin - biosynthesis</topic><topic>Receptors, Thrombin - genetics</topic><topic>Receptors, Thrombin - metabolism</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Thrombin - biosynthesis</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ROACH, Lindsay E</creatorcontrib><creatorcontrib>PETRIK, James J</creatorcontrib><creatorcontrib>PLANTE, Louise</creatorcontrib><creatorcontrib>LAMARRE, Jonathan</creatorcontrib><creatorcontrib>GENTRY, Patricia A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ROACH, Lindsay E</au><au>PETRIK, James J</au><au>PLANTE, Louise</au><au>LAMARRE, Jonathan</au><au>GENTRY, Patricia A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Thrombin Generation and Presence of Thrombin Receptor in Ovarian Follicles</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>2002-05-01</date><risdate>2002</risdate><volume>66</volume><issue>5</issue><spage>1350</spage><epage>1358</epage><pages>1350-1358</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>Prothrombin, once converted to its enzymatically active form (i.e., thrombin), induces a broad spectrum of cellular responses
in both vascular and avascular tissues. Bovine ovarian granulosa cells isolated from healthy follicles of various sizes contain
both prothrombin mRNA and immunologically reactive prothrombin that appears to be identical to prothrombin in follicular fluid
and plasma. When tissue factor, the primary physiological activator of thrombin generation in plasma, is used to initiate
thrombin formation, the profile of prothrombin-to-thrombin conversion is similar in follicular fluid and plasma. The conclusion
that biologically functional prothrombin is synthesized by granulosa cells is further supported by evidence that mRNA for
γ-glutamyl carboxylase, an enzyme essential for the vitamin K-dependent posttranslational modification of prothrombin, is
expressed in granulosa cells in a manner similar to prothrombin mRNA. Thrombin's biological effects are mediated through selective
proteolytic cleavage and activation of specific receptors. Bovine granulosa cells possess thrombin receptor (PAR-1) mRNA,
and as seen with prothrombin mRNA and γ-glutamyl carboxylase mRNA, cells isolated from small follicles possess more PAR-1
mRNA than cells from large follicles. Thrombin receptor expression by cells in close proximity to an active thrombin-generating
system suggests that these factors may be important mediators of cellular function in the ovarian follicle.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>11967197</pmid><doi>10.1095/biolreprod66.5.1350</doi><tpages>9</tpages></addata></record> |
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| source | MEDLINE; BioOne Complete; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Biological and medical sciences Blotting, Northern Blotting, Western Carbon-Carbon Ligases - metabolism Cattle Cell Separation Electrophoresis, Polyacrylamide Gel Female Follicular Fluid - chemistry Fundamental and applied biological sciences. Psychology Granulosa Cells - metabolism Humans In Vitro Techniques Mammalian female genital system Morphology. Physiology Ovarian Follicle - metabolism Prothrombin - metabolism Receptors, Thrombin - biosynthesis Receptors, Thrombin - genetics Receptors, Thrombin - metabolism RNA, Messenger - biosynthesis Thrombin - biosynthesis Vertebrates: reproduction |
| title | Thrombin Generation and Presence of Thrombin Receptor in Ovarian Follicles |
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