In-vitro circadian rhythm of murine bone marrow progenitor production
Hematopoietic processes display 24h rhythms both in rodents and in human beings. We hypothesized these rhythms to be in part generated by a circadian oscillator within the bone marrow. The ability of murine bone marrow granulo-monocytic (GM) precursors to form colonies following colony-stimulating f...
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| Veröffentlicht in: | Chronobiology international 2002, Vol.19 (1), p.57-67 |
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| container_title | Chronobiology international |
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| creator | Bourin, Philippe Ledain, Arnaud F. Beau, Jacques Mille, Dominique Lévi, Francis |
| description | Hematopoietic processes display 24h rhythms both in rodents and in human beings. We hypothesized these rhythms to be in part generated by a circadian oscillator within the bone marrow. The ability of murine bone marrow granulo-monocytic (GM) precursors to form colonies following colony-stimulating factor (rm GM-CSF) exposure was investigated in liquid culture samples obtained every 3 h for a span of up to 198 h. The CFU-GM count varied rhythmically over the first 4 d of culture, with a reproducible maximum in the early morning hours, similar to that observed in vivo. These experiments provide the first evidence that bone marrow progenitors sustain in vitro circadian rhythmicity, and they demonstrate the presence of a circadian time-keeping system within these cells. The results support the potential usefulness of bone marrow cultures for investigating chronopharmacologic effects of anticancer drugs and cytokines on this target system. |
| doi_str_mv | 10.1081/CBI-120002677 |
| format | Article |
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We hypothesized these rhythms to be in part generated by a circadian oscillator within the bone marrow. The ability of murine bone marrow granulo-monocytic (GM) precursors to form colonies following colony-stimulating factor (rm GM-CSF) exposure was investigated in liquid culture samples obtained every 3 h for a span of up to 198 h. The CFU-GM count varied rhythmically over the first 4 d of culture, with a reproducible maximum in the early morning hours, similar to that observed in vivo. These experiments provide the first evidence that bone marrow progenitors sustain in vitro circadian rhythmicity, and they demonstrate the presence of a circadian time-keeping system within these cells. The results support the potential usefulness of bone marrow cultures for investigating chronopharmacologic effects of anticancer drugs and cytokines on this target system.</description><subject>Animals</subject><subject>Chronotherapy</subject><subject>Circadian Rhythm - physiology</subject><subject>Colony-Forming Units Assay</subject><subject>Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology</subject><subject>Hematopoiesis - drug effects</subject><subject>Hematopoiesis - physiology</subject><subject>Hematopoietic Stem Cells - cytology</subject><subject>Hematopoietic Stem Cells - drug effects</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Recombinant Proteins - pharmacology</subject><issn>0742-0528</issn><issn>1525-6073</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kDtP7DAQRi10ESyPkhalul3Aj8R2SljxWAmJBmrLdmzWKLG54wS0_x6jXV1EQeNxceabmYPQGcEXBEtyubxe1YRijCkXYg8tSEvbmmPB_qAFFg2tcUvlITrK-bVAEnN2gA4J6Tjlki_QzSrW72GCVNkAVvdBxwrWm2k9VslX4wwhusqk8owaIH1Ub5BeXAxTgq9vP9sppHiC9r0esjvd1WP0fHvztLyvHx7vVsurh9oyyadadL5zTnPjBcctwV5700ghnLDOWM-saKkXsjeNcB3TtGmNbozWxjDcuIaxY_R3m1tG_5tdntQYsnXDoKNLc1aCcNzJlhaw3oIWUs7gvHqDUC7YKILVlzdVvKn_3gp_vguezej6b3onqgByC4ToE4z6I8HQq0lvhgQedLQhK_ZbtvjRunZ6mNZWg1OvaYZYhP2y1Scmmo3y</recordid><startdate>2002</startdate><enddate>2002</enddate><creator>Bourin, Philippe</creator><creator>Ledain, Arnaud F.</creator><creator>Beau, Jacques</creator><creator>Mille, Dominique</creator><creator>Lévi, Francis</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2002</creationdate><title>In-vitro circadian rhythm of murine bone marrow progenitor production</title><author>Bourin, Philippe ; Ledain, Arnaud F. ; Beau, Jacques ; Mille, Dominique ; Lévi, Francis</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-79f9eea6bf760510fafb4877e7cebcf3c752f78db47e93a245ba4baabb304e433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Chronotherapy</topic><topic>Circadian Rhythm - physiology</topic><topic>Colony-Forming Units Assay</topic><topic>Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology</topic><topic>Hematopoiesis - drug effects</topic><topic>Hematopoiesis - physiology</topic><topic>Hematopoietic Stem Cells - cytology</topic><topic>Hematopoietic Stem Cells - drug effects</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Recombinant Proteins - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bourin, Philippe</creatorcontrib><creatorcontrib>Ledain, Arnaud F.</creatorcontrib><creatorcontrib>Beau, Jacques</creatorcontrib><creatorcontrib>Mille, Dominique</creatorcontrib><creatorcontrib>Lévi, Francis</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Chronobiology international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bourin, Philippe</au><au>Ledain, Arnaud F.</au><au>Beau, Jacques</au><au>Mille, Dominique</au><au>Lévi, Francis</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In-vitro circadian rhythm of murine bone marrow progenitor production</atitle><jtitle>Chronobiology international</jtitle><addtitle>Chronobiol Int</addtitle><date>2002</date><risdate>2002</risdate><volume>19</volume><issue>1</issue><spage>57</spage><epage>67</epage><pages>57-67</pages><issn>0742-0528</issn><eissn>1525-6073</eissn><abstract>Hematopoietic processes display 24h rhythms both in rodents and in human beings. 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| ispartof | Chronobiology international, 2002, Vol.19 (1), p.57-67 |
| issn | 0742-0528 1525-6073 |
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| source | MEDLINE; Taylor & Francis:Master (3349 titles) |
| subjects | Animals Chronotherapy Circadian Rhythm - physiology Colony-Forming Units Assay Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology Hematopoiesis - drug effects Hematopoiesis - physiology Hematopoietic Stem Cells - cytology Hematopoietic Stem Cells - drug effects Humans In Vitro Techniques Male Mice Mice, Inbred BALB C Recombinant Proteins - pharmacology |
| title | In-vitro circadian rhythm of murine bone marrow progenitor production |
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