25-Hydroxycholesterol Is Produced by Testicular Macrophages During the Early Postnatal Period and Influences Differentiation of Leydig Cells In Vitro
Leydig cells develop inappropriately in animals lacking testicular macrophages. We have recently found that macrophages from adult animals produce 25-hydroxycholesterol, an oxysterol involved in the differentiation of hepatocytes and keratinocytes. Therefore, we hypothesized that testicular macropha...
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| Veröffentlicht in: | Biology of reproduction 2002-05, Vol.66 (5), p.1336-1341 |
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| creator | CHEN, Jau-Jiin LUKYANENKO, Yevgeniya HUTSON, James C |
| description | Leydig cells develop inappropriately in animals lacking testicular macrophages. We have recently found that macrophages from
adult animals produce 25-hydroxycholesterol, an oxysterol involved in the differentiation of hepatocytes and keratinocytes.
Therefore, we hypothesized that testicular macrophages also produce 25-hydroxycholesterol during the early postnatal period
and that this oxysterol plays a role in the differentiation of Leydig cells. We assessed the production of 25-hydroxycholesterol
and 25-hydroxylase mRNA by cultured testicular macrophages from rats at 10, 20, and 40 days of age. We also tested the long-term
effects of 25-hydroxycholesterol on basal and LH-stimulated testosterone production, and 3β-hydroxysteroid dehydrogenase activity
as end points of Leydig cell differentiation in vitro. We found that testicular macrophages from animals at all ages produced
both 25-hydroxycholesterol and 25-hydroxylase mRNA, with macrophages from 10-day-old animals having the highest steady-state
levels of message. We also found that chronic exposure of Leydig cells to 25-hydroxycholesterol increased basal production
of testosterone but decreased LH-stimulated steroidogenesis at all ages. Finally, 25-hydroxycholesterol increased 3β-hydroxysteroid
dehydrogenase activity in both progenitor and immature Leydig cells. These findings support the hypothesis that testicular
macrophages play an important role in the differentiation of Leydig cells through the secretion of 25-hydroxycholesterol. |
| doi_str_mv | 10.1095/biolreprod66.5.1336 |
| format | Article |
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adult animals produce 25-hydroxycholesterol, an oxysterol involved in the differentiation of hepatocytes and keratinocytes.
Therefore, we hypothesized that testicular macrophages also produce 25-hydroxycholesterol during the early postnatal period
and that this oxysterol plays a role in the differentiation of Leydig cells. We assessed the production of 25-hydroxycholesterol
and 25-hydroxylase mRNA by cultured testicular macrophages from rats at 10, 20, and 40 days of age. We also tested the long-term
effects of 25-hydroxycholesterol on basal and LH-stimulated testosterone production, and 3β-hydroxysteroid dehydrogenase activity
as end points of Leydig cell differentiation in vitro. We found that testicular macrophages from animals at all ages produced
both 25-hydroxycholesterol and 25-hydroxylase mRNA, with macrophages from 10-day-old animals having the highest steady-state
levels of message. We also found that chronic exposure of Leydig cells to 25-hydroxycholesterol increased basal production
of testosterone but decreased LH-stimulated steroidogenesis at all ages. Finally, 25-hydroxycholesterol increased 3β-hydroxysteroid
dehydrogenase activity in both progenitor and immature Leydig cells. These findings support the hypothesis that testicular
macrophages play an important role in the differentiation of Leydig cells through the secretion of 25-hydroxycholesterol.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod66.5.1336</identifier><identifier>PMID: 11967195</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>3-Hydroxysteroid Dehydrogenases - biosynthesis ; Animals ; Animals, Newborn - metabolism ; Biological and medical sciences ; Cell Differentiation - drug effects ; Cells, Cultured ; Culture Media ; Fundamental and applied biological sciences. Psychology ; Hydroxycholesterols - metabolism ; Leydig Cells - metabolism ; Macrophages - metabolism ; Male ; Mammalian male genital system ; Morphology. Physiology ; Rats ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - biosynthesis ; Testis - cytology ; Testis - metabolism ; Testosterone - biosynthesis ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 2002-05, Vol.66 (5), p.1336-1341</ispartof><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13628857$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11967195$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>CHEN, Jau-Jiin</creatorcontrib><creatorcontrib>LUKYANENKO, Yevgeniya</creatorcontrib><creatorcontrib>HUTSON, James C</creatorcontrib><title>25-Hydroxycholesterol Is Produced by Testicular Macrophages During the Early Postnatal Period and Influences Differentiation of Leydig Cells In Vitro</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>Leydig cells develop inappropriately in animals lacking testicular macrophages. We have recently found that macrophages from
adult animals produce 25-hydroxycholesterol, an oxysterol involved in the differentiation of hepatocytes and keratinocytes.
Therefore, we hypothesized that testicular macrophages also produce 25-hydroxycholesterol during the early postnatal period
and that this oxysterol plays a role in the differentiation of Leydig cells. We assessed the production of 25-hydroxycholesterol
and 25-hydroxylase mRNA by cultured testicular macrophages from rats at 10, 20, and 40 days of age. We also tested the long-term
effects of 25-hydroxycholesterol on basal and LH-stimulated testosterone production, and 3β-hydroxysteroid dehydrogenase activity
as end points of Leydig cell differentiation in vitro. We found that testicular macrophages from animals at all ages produced
both 25-hydroxycholesterol and 25-hydroxylase mRNA, with macrophages from 10-day-old animals having the highest steady-state
levels of message. We also found that chronic exposure of Leydig cells to 25-hydroxycholesterol increased basal production
of testosterone but decreased LH-stimulated steroidogenesis at all ages. Finally, 25-hydroxycholesterol increased 3β-hydroxysteroid
dehydrogenase activity in both progenitor and immature Leydig cells. These findings support the hypothesis that testicular
macrophages play an important role in the differentiation of Leydig cells through the secretion of 25-hydroxycholesterol.</description><subject>3-Hydroxysteroid Dehydrogenases - biosynthesis</subject><subject>Animals</subject><subject>Animals, Newborn - metabolism</subject><subject>Biological and medical sciences</subject><subject>Cell Differentiation - drug effects</subject><subject>Cells, Cultured</subject><subject>Culture Media</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydroxycholesterols - metabolism</subject><subject>Leydig Cells - metabolism</subject><subject>Macrophages - metabolism</subject><subject>Male</subject><subject>Mammalian male genital system</subject><subject>Morphology. Physiology</subject><subject>Rats</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Testis - cytology</subject><subject>Testis - metabolism</subject><subject>Testosterone - biosynthesis</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkc-O0zAQxi0EYrsLT4CEfIFbih3Hjn1E3WW3UhE9LFwj_5k0Rm5c7EQlD8L74hVFexpp9Jtv5vsGoXeUrClR_JPxMSQ4peiEWPM1ZUy8QCvKa1W1tZAv0YoQIqrSZlfoOuefhNCG1ew1uqJUiZYqvkJ_al49LC7F34sdYoA8QYoBbzPeF-HZgsNmwY-l7-0cdMJftU3xNOgDZHw7Jz8e8DQAvtMpLHgf8zTqSQe8h-Sjw3p0eDv2YYbRPg34vocE4-T15OOIY493sDh_wBsIIRcU__BTim_Qq16HDG8v9QZ9_3L3uHmodt_ut5vPu2ooBqdKaV0zzoSQqpEAIFrZGNsqrq1sSFOr1hpFwfROyt6ZVjFlGka5c7Uqc4bdoI__dEuKv-Zisjv6bMspeoQ4566lgiihWAHfX8DZHMF1p-SPOi3d_yAL8OEC6Gx16JMerc_PHBO1lLx93jj4w3D2Cbp81CEUWdadz2chOt49fZL9BaEuk-A</recordid><startdate>20020501</startdate><enddate>20020501</enddate><creator>CHEN, Jau-Jiin</creator><creator>LUKYANENKO, Yevgeniya</creator><creator>HUTSON, James C</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20020501</creationdate><title>25-Hydroxycholesterol Is Produced by Testicular Macrophages During the Early Postnatal Period and Influences Differentiation of Leydig Cells In Vitro</title><author>CHEN, Jau-Jiin ; LUKYANENKO, Yevgeniya ; HUTSON, James C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h268t-9aa2353668948eee6784bc795ac8404297cb91ebfd88fdb7939b4315dd29353b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>3-Hydroxysteroid Dehydrogenases - biosynthesis</topic><topic>Animals</topic><topic>Animals, Newborn - metabolism</topic><topic>Biological and medical sciences</topic><topic>Cell Differentiation - drug effects</topic><topic>Cells, Cultured</topic><topic>Culture Media</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydroxycholesterols - metabolism</topic><topic>Leydig Cells - metabolism</topic><topic>Macrophages - metabolism</topic><topic>Male</topic><topic>Mammalian male genital system</topic><topic>Morphology. Physiology</topic><topic>Rats</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Testis - cytology</topic><topic>Testis - metabolism</topic><topic>Testosterone - biosynthesis</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CHEN, Jau-Jiin</creatorcontrib><creatorcontrib>LUKYANENKO, Yevgeniya</creatorcontrib><creatorcontrib>HUTSON, James C</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CHEN, Jau-Jiin</au><au>LUKYANENKO, Yevgeniya</au><au>HUTSON, James C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>25-Hydroxycholesterol Is Produced by Testicular Macrophages During the Early Postnatal Period and Influences Differentiation of Leydig Cells In Vitro</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>2002-05-01</date><risdate>2002</risdate><volume>66</volume><issue>5</issue><spage>1336</spage><epage>1341</epage><pages>1336-1341</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>Leydig cells develop inappropriately in animals lacking testicular macrophages. We have recently found that macrophages from
adult animals produce 25-hydroxycholesterol, an oxysterol involved in the differentiation of hepatocytes and keratinocytes.
Therefore, we hypothesized that testicular macrophages also produce 25-hydroxycholesterol during the early postnatal period
and that this oxysterol plays a role in the differentiation of Leydig cells. We assessed the production of 25-hydroxycholesterol
and 25-hydroxylase mRNA by cultured testicular macrophages from rats at 10, 20, and 40 days of age. We also tested the long-term
effects of 25-hydroxycholesterol on basal and LH-stimulated testosterone production, and 3β-hydroxysteroid dehydrogenase activity
as end points of Leydig cell differentiation in vitro. We found that testicular macrophages from animals at all ages produced
both 25-hydroxycholesterol and 25-hydroxylase mRNA, with macrophages from 10-day-old animals having the highest steady-state
levels of message. We also found that chronic exposure of Leydig cells to 25-hydroxycholesterol increased basal production
of testosterone but decreased LH-stimulated steroidogenesis at all ages. Finally, 25-hydroxycholesterol increased 3β-hydroxysteroid
dehydrogenase activity in both progenitor and immature Leydig cells. These findings support the hypothesis that testicular
macrophages play an important role in the differentiation of Leydig cells through the secretion of 25-hydroxycholesterol.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>11967195</pmid><doi>10.1095/biolreprod66.5.1336</doi><tpages>6</tpages></addata></record> |
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| source | MEDLINE; Oxford University Press Journals Current; EZB-FREE-00999 freely available EZB journals; BioOne Complete |
| subjects | 3-Hydroxysteroid Dehydrogenases - biosynthesis Animals Animals, Newborn - metabolism Biological and medical sciences Cell Differentiation - drug effects Cells, Cultured Culture Media Fundamental and applied biological sciences. Psychology Hydroxycholesterols - metabolism Leydig Cells - metabolism Macrophages - metabolism Male Mammalian male genital system Morphology. Physiology Rats Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - biosynthesis Testis - cytology Testis - metabolism Testosterone - biosynthesis Vertebrates: reproduction |
| title | 25-Hydroxycholesterol Is Produced by Testicular Macrophages During the Early Postnatal Period and Influences Differentiation of Leydig Cells In Vitro |
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