A hybrid Bacillus thuringiensis delta-endotoxin gives resistance against a coleopteran and a lepidopteran pest in transgenic potato
Summary Expression of Bacillus thuringiensis delta‐endotoxins has proven to be a successful strategy for obtaining insect resistance in transgenic plants. Drawbacks of expression of a single resistance gene are the limited target spectrum and the potential for rapid adaptation of the pest. Hybrid to...
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| Veröffentlicht in: | Plant biotechnology journal 2003-01, Vol.1 (1), p.51-57 |
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| container_title | Plant biotechnology journal |
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| creator | Naimov, Samir Dukiandjiev, Stefan De Maagd, Ruud A. |
| description | Summary
Expression of Bacillus thuringiensis delta‐endotoxins has proven to be a successful strategy for obtaining insect resistance in transgenic plants. Drawbacks of expression of a single resistance gene are the limited target spectrum and the potential for rapid adaptation of the pest. Hybrid toxins with a wider target spectrum in combination with existing toxins may be used as tool to mitigate these problems.
In this study, Desiree potato plants were genetically modified to resist attack by insect species belonging to the orders Coleoptera and Lepidoptera, through the insertion of such a hybrid gene, SN19. Transgenic plants were shown to be resistant against Colorado potato beetle larvae and adults, potato tuber moth larvae, and European corn borer larvae. These are the first transgenic plants resistant to pests belonging to two different insect orders. In addition, the target receptor recognition of this hybrid protein is expected to be different from Cry proteins currently in use for these pests. This makes it a useful tool for resistance management strategies. |
| doi_str_mv | 10.1046/j.1467-7652.2003.00005.x |
| format | Article |
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Expression of Bacillus thuringiensis delta‐endotoxins has proven to be a successful strategy for obtaining insect resistance in transgenic plants. Drawbacks of expression of a single resistance gene are the limited target spectrum and the potential for rapid adaptation of the pest. Hybrid toxins with a wider target spectrum in combination with existing toxins may be used as tool to mitigate these problems.
In this study, Desiree potato plants were genetically modified to resist attack by insect species belonging to the orders Coleoptera and Lepidoptera, through the insertion of such a hybrid gene, SN19. Transgenic plants were shown to be resistant against Colorado potato beetle larvae and adults, potato tuber moth larvae, and European corn borer larvae. These are the first transgenic plants resistant to pests belonging to two different insect orders. In addition, the target receptor recognition of this hybrid protein is expected to be different from Cry proteins currently in use for these pests. This makes it a useful tool for resistance management strategies.</description><identifier>ISSN: 1467-7644</identifier><identifier>EISSN: 1467-7652</identifier><identifier>DOI: 10.1046/j.1467-7652.2003.00005.x</identifier><identifier>PMID: 17147680</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Bacillus thuringiensis ; Coleoptera ; Colorado potato beetle ; European corn borer ; hybrid Cry1 protein ; Lepidoptera ; Ostrinia nubilalis ; potato tuber moth ; Solanum tuberosum ; transgenic potato plants</subject><ispartof>Plant biotechnology journal, 2003-01, Vol.1 (1), p.51-57</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3985-87c09c97bc303637873de0634ada35c39800922f852a72d40293200d2ea21df83</citedby><cites>FETCH-LOGICAL-c3985-87c09c97bc303637873de0634ada35c39800922f852a72d40293200d2ea21df83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1467-7652.2003.00005.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1467-7652.2003.00005.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,11542,27903,27904,45553,45554,46030,46454</link.rule.ids><linktorsrc>$$Uhttps://onlinelibrary.wiley.com/doi/abs/10.1046%2Fj.1467-7652.2003.00005.x$$EView_record_in_Wiley-Blackwell$$FView_record_in_$$GWiley-Blackwell</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17147680$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Naimov, Samir</creatorcontrib><creatorcontrib>Dukiandjiev, Stefan</creatorcontrib><creatorcontrib>De Maagd, Ruud A.</creatorcontrib><title>A hybrid Bacillus thuringiensis delta-endotoxin gives resistance against a coleopteran and a lepidopteran pest in transgenic potato</title><title>Plant biotechnology journal</title><addtitle>Plant Biotechnol J</addtitle><description>Summary
Expression of Bacillus thuringiensis delta‐endotoxins has proven to be a successful strategy for obtaining insect resistance in transgenic plants. Drawbacks of expression of a single resistance gene are the limited target spectrum and the potential for rapid adaptation of the pest. Hybrid toxins with a wider target spectrum in combination with existing toxins may be used as tool to mitigate these problems.
In this study, Desiree potato plants were genetically modified to resist attack by insect species belonging to the orders Coleoptera and Lepidoptera, through the insertion of such a hybrid gene, SN19. Transgenic plants were shown to be resistant against Colorado potato beetle larvae and adults, potato tuber moth larvae, and European corn borer larvae. These are the first transgenic plants resistant to pests belonging to two different insect orders. In addition, the target receptor recognition of this hybrid protein is expected to be different from Cry proteins currently in use for these pests. This makes it a useful tool for resistance management strategies.</description><subject>Bacillus thuringiensis</subject><subject>Coleoptera</subject><subject>Colorado potato beetle</subject><subject>European corn borer</subject><subject>hybrid Cry1 protein</subject><subject>Lepidoptera</subject><subject>Ostrinia nubilalis</subject><subject>potato tuber moth</subject><subject>Solanum tuberosum</subject><subject>transgenic potato plants</subject><issn>1467-7644</issn><issn>1467-7652</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNqNkc1u1DAUhSMEoqXwCsgrdgn-i51IbNoplEpVyQKExMby2HemHjJOsB2YWfPiOMwwLMEbX9_7nWPLpygQwRXBXLzeVIQLWUpR04pizCqcV13tHhXnp8HjU835WfEsxg3GlIhaPC3OiCRcigafFz8v0cN-GZxFV9q4vp8iSg9TcH7twEcXkYU-6RK8HdKwcx6t3XeIKECeJe0NIL3WzseENDJDD8OYIGiPtLe508Po7J_WCJnKDikf4hq8M2gckk7D8-LJSvcRXhz3i-LTu7cfF-_Luw83t4vLu9KwtqnLRhrcmlYuDcNMMNlIZgELxrXVrJ4ZjFtKV01NtaSWY9qy_DmWgqbErhp2Ubw6-I5h-Dbl16itiwb6XnsYpqgkEVhy8m-QyJq3TUsy2BxAE4YYA6zUGNxWh70iWM1JqY2aQ1BzIGpOSv1OSu2y9OXxjmm5BftXeIwmA28OwA_Xw_6_jVV3dZuLLC8P8pwT7E5yHb4qIZms1ef7G7Xo7rvuuhPqC_sFiLOybw</recordid><startdate>200301</startdate><enddate>200301</enddate><creator>Naimov, Samir</creator><creator>Dukiandjiev, Stefan</creator><creator>De Maagd, Ruud A.</creator><general>Blackwell Science Ltd</general><scope>BSCLL</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7SS</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200301</creationdate><title>A hybrid Bacillus thuringiensis delta-endotoxin gives resistance against a coleopteran and a lepidopteran pest in transgenic potato</title><author>Naimov, Samir ; Dukiandjiev, Stefan ; De Maagd, Ruud A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3985-87c09c97bc303637873de0634ada35c39800922f852a72d40293200d2ea21df83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Bacillus thuringiensis</topic><topic>Coleoptera</topic><topic>Colorado potato beetle</topic><topic>European corn borer</topic><topic>hybrid Cry1 protein</topic><topic>Lepidoptera</topic><topic>Ostrinia nubilalis</topic><topic>potato tuber moth</topic><topic>Solanum tuberosum</topic><topic>transgenic potato plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Naimov, Samir</creatorcontrib><creatorcontrib>Dukiandjiev, Stefan</creatorcontrib><creatorcontrib>De Maagd, Ruud A.</creatorcontrib><collection>Istex</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant biotechnology journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Naimov, Samir</au><au>Dukiandjiev, Stefan</au><au>De Maagd, Ruud A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A hybrid Bacillus thuringiensis delta-endotoxin gives resistance against a coleopteran and a lepidopteran pest in transgenic potato</atitle><jtitle>Plant biotechnology journal</jtitle><addtitle>Plant Biotechnol J</addtitle><date>2003-01</date><risdate>2003</risdate><volume>1</volume><issue>1</issue><spage>51</spage><epage>57</epage><pages>51-57</pages><issn>1467-7644</issn><eissn>1467-7652</eissn><abstract>Summary
Expression of Bacillus thuringiensis delta‐endotoxins has proven to be a successful strategy for obtaining insect resistance in transgenic plants. Drawbacks of expression of a single resistance gene are the limited target spectrum and the potential for rapid adaptation of the pest. Hybrid toxins with a wider target spectrum in combination with existing toxins may be used as tool to mitigate these problems.
In this study, Desiree potato plants were genetically modified to resist attack by insect species belonging to the orders Coleoptera and Lepidoptera, through the insertion of such a hybrid gene, SN19. Transgenic plants were shown to be resistant against Colorado potato beetle larvae and adults, potato tuber moth larvae, and European corn borer larvae. These are the first transgenic plants resistant to pests belonging to two different insect orders. In addition, the target receptor recognition of this hybrid protein is expected to be different from Cry proteins currently in use for these pests. This makes it a useful tool for resistance management strategies.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>17147680</pmid><doi>10.1046/j.1467-7652.2003.00005.x</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Plant biotechnology journal, 2003-01, Vol.1 (1), p.51-57 |
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| language | eng |
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| subjects | Bacillus thuringiensis Coleoptera Colorado potato beetle European corn borer hybrid Cry1 protein Lepidoptera Ostrinia nubilalis potato tuber moth Solanum tuberosum transgenic potato plants |
| title | A hybrid Bacillus thuringiensis delta-endotoxin gives resistance against a coleopteran and a lepidopteran pest in transgenic potato |
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