Site-independently integrated transgenes in the elite restorer rice line Minghui 63 allow removal of a selectable marker from the gene of interest by self-segregation
Summary In this study, we have demonstrated that two independent loci are involved in the integration of the insecticidal protein gene cryIAb/cryIAc and selectable marker gene hph in the recipient genome of the elite Chinese CMS restorer line Minghui 63. We have also documented the structural organi...
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| Veröffentlicht in: | Plant biotechnology journal 2003-05, Vol.1 (3), p.155-165 |
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| container_title | Plant biotechnology journal |
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| creator | Tu, Jumin Datta, Karabi Oliva, Norman Zhang, Guoan Xu, Caiguo Khush, Gurdev Singh Zhang, Qifa Datta, Swapan Kumar |
| description | Summary
In this study, we have demonstrated that two independent loci are involved in the integration of the insecticidal protein gene cryIAb/cryIAc and selectable marker gene hph in the recipient genome of the elite Chinese CMS restorer line Minghui 63. We have also documented the structural organization of these transgenes in each locus by restriction enzyme digestion and Southern blot analysis. The independent locus integration of different transgenes allowed us to remove the selectable marker gene hph from the gene of interest simply by self‐segregation. Not having the selectable marker gene will enhance the commercial value of our transgenic line TT51‐1, which showed a consistently high level of resistance against repeated infestations of yellow stem borers and natural outbreaks of leaf‐folders, without a reduction in yield potential. |
| doi_str_mv | 10.1046/j.1467-7652.2003.00012.x |
| format | Article |
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In this study, we have demonstrated that two independent loci are involved in the integration of the insecticidal protein gene cryIAb/cryIAc and selectable marker gene hph in the recipient genome of the elite Chinese CMS restorer line Minghui 63. We have also documented the structural organization of these transgenes in each locus by restriction enzyme digestion and Southern blot analysis. The independent locus integration of different transgenes allowed us to remove the selectable marker gene hph from the gene of interest simply by self‐segregation. Not having the selectable marker gene will enhance the commercial value of our transgenic line TT51‐1, which showed a consistently high level of resistance against repeated infestations of yellow stem borers and natural outbreaks of leaf‐folders, without a reduction in yield potential.</description><identifier>ISSN: 1467-7644</identifier><identifier>EISSN: 1467-7652</identifier><identifier>DOI: 10.1046/j.1467-7652.2003.00012.x</identifier><identifier>PMID: 17156029</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>cryIAb/ cryIAc ; hybrid rice ; independent locus integration ; insect resistance ; marker-free transgenic plant ; Oryza sativa ; restorer</subject><ispartof>Plant biotechnology journal, 2003-05, Vol.1 (3), p.155-165</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4992-1026277c9443f51b514f8d2ff1c7b0de484eaa47117b75444cafbc28e28369333</citedby><cites>FETCH-LOGICAL-c4992-1026277c9443f51b514f8d2ff1c7b0de484eaa47117b75444cafbc28e28369333</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1467-7652.2003.00012.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1467-7652.2003.00012.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,11562,27924,27925,45574,45575,46052,46476</link.rule.ids><linktorsrc>$$Uhttps://onlinelibrary.wiley.com/doi/abs/10.1046%2Fj.1467-7652.2003.00012.x$$EView_record_in_Wiley-Blackwell$$FView_record_in_$$GWiley-Blackwell</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17156029$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tu, Jumin</creatorcontrib><creatorcontrib>Datta, Karabi</creatorcontrib><creatorcontrib>Oliva, Norman</creatorcontrib><creatorcontrib>Zhang, Guoan</creatorcontrib><creatorcontrib>Xu, Caiguo</creatorcontrib><creatorcontrib>Khush, Gurdev Singh</creatorcontrib><creatorcontrib>Zhang, Qifa</creatorcontrib><creatorcontrib>Datta, Swapan Kumar</creatorcontrib><title>Site-independently integrated transgenes in the elite restorer rice line Minghui 63 allow removal of a selectable marker from the gene of interest by self-segregation</title><title>Plant biotechnology journal</title><addtitle>Plant Biotechnol J</addtitle><description>Summary
In this study, we have demonstrated that two independent loci are involved in the integration of the insecticidal protein gene cryIAb/cryIAc and selectable marker gene hph in the recipient genome of the elite Chinese CMS restorer line Minghui 63. We have also documented the structural organization of these transgenes in each locus by restriction enzyme digestion and Southern blot analysis. The independent locus integration of different transgenes allowed us to remove the selectable marker gene hph from the gene of interest simply by self‐segregation. Not having the selectable marker gene will enhance the commercial value of our transgenic line TT51‐1, which showed a consistently high level of resistance against repeated infestations of yellow stem borers and natural outbreaks of leaf‐folders, without a reduction in yield potential.</description><subject>cryIAb/ cryIAc</subject><subject>hybrid rice</subject><subject>independent locus integration</subject><subject>insect resistance</subject><subject>marker-free transgenic plant</subject><subject>Oryza sativa</subject><subject>restorer</subject><issn>1467-7644</issn><issn>1467-7652</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNqNkc1u1DAUhSMEoqXwCsgrdgm249iJxAZaaCsNf-J3ZznO9dSDkwy2h868EM-J3RkNS9jYV9ffOffKpygQwRXBjD9fVYRxUQre0IpiXFcYY0Kr7b3i9Phw_1gzdlI8CmGFMSW84Q-LEyJIwzHtTovfn2yE0k4DrCEdU3Q7ZKcIS68iDCh6NYUlTBBSF8UbQOCSAHkIcfbgkbcakLMToLd2Wt5sLOI1Us7Nt4kZ51_KodkghQI40FH1DtCo_I-kNH4e7xyzfYby2OyL-l3GTRnSFrBU0c7T4-KBUS7Ak8N9Vnx58_rz-VW5eH95ff5yUWrWdbQkmHIqhO4Yq01D-oYw0w7UGKJFjwdgLQOlmCBE9KJhjGllek1boG3Nu7quz4pne9-1n39u0jJytEGDc2qCeROkIBwLLP4NEtFwJihNYLsHtZ9D8GDk2tv0BTtJsMxhypXMOcmcmcxhyrsw5TZJnx5mbPoRhr_CQ3oJeLEHbq2D3X8byw-vrlOR5OVebkOE7VGe4pFc1KKR395dyu9X4uIj_7qQov4DJbG-zw</recordid><startdate>200305</startdate><enddate>200305</enddate><creator>Tu, Jumin</creator><creator>Datta, Karabi</creator><creator>Oliva, Norman</creator><creator>Zhang, Guoan</creator><creator>Xu, Caiguo</creator><creator>Khush, Gurdev Singh</creator><creator>Zhang, Qifa</creator><creator>Datta, Swapan Kumar</creator><general>Blackwell Science Ltd</general><scope>BSCLL</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200305</creationdate><title>Site-independently integrated transgenes in the elite restorer rice line Minghui 63 allow removal of a selectable marker from the gene of interest by self-segregation</title><author>Tu, Jumin ; Datta, Karabi ; Oliva, Norman ; Zhang, Guoan ; Xu, Caiguo ; Khush, Gurdev Singh ; Zhang, Qifa ; Datta, Swapan Kumar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4992-1026277c9443f51b514f8d2ff1c7b0de484eaa47117b75444cafbc28e28369333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>cryIAb/ cryIAc</topic><topic>hybrid rice</topic><topic>independent locus integration</topic><topic>insect resistance</topic><topic>marker-free transgenic plant</topic><topic>Oryza sativa</topic><topic>restorer</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tu, Jumin</creatorcontrib><creatorcontrib>Datta, Karabi</creatorcontrib><creatorcontrib>Oliva, Norman</creatorcontrib><creatorcontrib>Zhang, Guoan</creatorcontrib><creatorcontrib>Xu, Caiguo</creatorcontrib><creatorcontrib>Khush, Gurdev Singh</creatorcontrib><creatorcontrib>Zhang, Qifa</creatorcontrib><creatorcontrib>Datta, Swapan Kumar</creatorcontrib><collection>Istex</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant biotechnology journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Tu, Jumin</au><au>Datta, Karabi</au><au>Oliva, Norman</au><au>Zhang, Guoan</au><au>Xu, Caiguo</au><au>Khush, Gurdev Singh</au><au>Zhang, Qifa</au><au>Datta, Swapan Kumar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Site-independently integrated transgenes in the elite restorer rice line Minghui 63 allow removal of a selectable marker from the gene of interest by self-segregation</atitle><jtitle>Plant biotechnology journal</jtitle><addtitle>Plant Biotechnol J</addtitle><date>2003-05</date><risdate>2003</risdate><volume>1</volume><issue>3</issue><spage>155</spage><epage>165</epage><pages>155-165</pages><issn>1467-7644</issn><eissn>1467-7652</eissn><abstract>Summary
In this study, we have demonstrated that two independent loci are involved in the integration of the insecticidal protein gene cryIAb/cryIAc and selectable marker gene hph in the recipient genome of the elite Chinese CMS restorer line Minghui 63. We have also documented the structural organization of these transgenes in each locus by restriction enzyme digestion and Southern blot analysis. The independent locus integration of different transgenes allowed us to remove the selectable marker gene hph from the gene of interest simply by self‐segregation. Not having the selectable marker gene will enhance the commercial value of our transgenic line TT51‐1, which showed a consistently high level of resistance against repeated infestations of yellow stem borers and natural outbreaks of leaf‐folders, without a reduction in yield potential.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>17156029</pmid><doi>10.1046/j.1467-7652.2003.00012.x</doi><tpages>11</tpages></addata></record> |
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| ispartof | Plant biotechnology journal, 2003-05, Vol.1 (3), p.155-165 |
| issn | 1467-7644 1467-7652 |
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| source | Wiley Online Library (Open Access Collection) |
| subjects | cryIAb/ cryIAc hybrid rice independent locus integration insect resistance marker-free transgenic plant Oryza sativa restorer |
| title | Site-independently integrated transgenes in the elite restorer rice line Minghui 63 allow removal of a selectable marker from the gene of interest by self-segregation |
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