Atce1: A Novel Mouse Cyclic Adenosine 3′,5′-Monophosphate-Responsive Element-Binding Protein-Like Gene Exclusively Expressed in Postmeiotic Spermatids
Members of the ATF/CREB family of transcription factors are involved in gene activation in various physiological systems ranging from metabolite homeostasis, through regulation of cell cycle, to learning and memory. Two members of this family, cAMP-responsive element binding protein (CREB), and cAMP...
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Veröffentlicht in: | Endocrinology (Philadelphia) 2002-05, Vol.143 (5), p.1578-1588 |
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description | Members of the ATF/CREB family of transcription factors are involved in gene activation in various physiological systems ranging from metabolite homeostasis, through regulation of cell cycle, to learning and memory. Two members of this family, cAMP-responsive element binding protein (CREB), and cAMP-responsive element modulator (CREM) are active during mammalian spermatogenesis and are required for this process, as has been shown by knockout and dominant negative experiments. In an effort to identify mouse proteins that interact with the testis-specific protein Tctex2, a mouse testis expression library was screened via the two-hybrid system, using the carboxyl-terminal portion of this protein as bait. A clone containing two overlapping open reading frames, related by a frameshift of one nucleotide, was subsequently isolated. The peptide that interacted with Tctex2 does not initiate from a consensus AUG codon, and it is not clear whether it exists physiologically. However, the other reading frame, initiating from an AUG codon, encodes a 315-amino acid peptide with significant sequence homology to a subfamily of the CREB genes whose prototype is the mouse LZIP peptide. This novel CREB-like peptide, designated Atce1, is specifically expressed in the testis. A developmental study using Northern hybridization and in situ hybridization analyses revealed that Atce1 transcripts begin to accumulate in testes of mice 24 d after birth, reflecting expression in mid/late round spermatids. Interestingly, EMSAs revealed that in vitro translated Atce1 binds specifically to a nuclear factor-κB-binding element rather then to a CRE element. Potential roles for Atce1 are discussed. |
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Two members of this family, cAMP-responsive element binding protein (CREB), and cAMP-responsive element modulator (CREM) are active during mammalian spermatogenesis and are required for this process, as has been shown by knockout and dominant negative experiments. In an effort to identify mouse proteins that interact with the testis-specific protein Tctex2, a mouse testis expression library was screened via the two-hybrid system, using the carboxyl-terminal portion of this protein as bait. A clone containing two overlapping open reading frames, related by a frameshift of one nucleotide, was subsequently isolated. The peptide that interacted with Tctex2 does not initiate from a consensus AUG codon, and it is not clear whether it exists physiologically. However, the other reading frame, initiating from an AUG codon, encodes a 315-amino acid peptide with significant sequence homology to a subfamily of the CREB genes whose prototype is the mouse LZIP peptide. This novel CREB-like peptide, designated Atce1, is specifically expressed in the testis. A developmental study using Northern hybridization and in situ hybridization analyses revealed that Atce1 transcripts begin to accumulate in testes of mice 24 d after birth, reflecting expression in mid/late round spermatids. Interestingly, EMSAs revealed that in vitro translated Atce1 binds specifically to a nuclear factor-κB-binding element rather then to a CRE element. Potential roles for Atce1 are discussed.</description><identifier>ISSN: 0013-7227</identifier><identifier>EISSN: 1945-7170</identifier><identifier>DOI: 10.1210/endo.143.5.8822</identifier><identifier>PMID: 11956138</identifier><language>eng</language><publisher>United States: Endocrine Society</publisher><subject>Adenosine ; Amino Acid Sequence ; Amino acids ; AMP ; Animals ; Baits ; Base Sequence ; Blotting, Northern ; Cell activation ; Cell cycle ; Chromosome Mapping ; Conserved sequence ; Cyclic AMP response element modulator ; Cyclic AMP response element-binding protein ; Cyclic AMP Response Element-Binding Protein - genetics ; DNA - metabolism ; Electrophoresis ; Gene Library ; Gene regulation ; Haploidy ; Homeostasis ; Homology ; Hybrid Cells ; Hybrid systems ; Hybridization ; In Situ Hybridization ; Male ; Meiosis - genetics ; Metabolites ; Mice ; Molecular Sequence Data ; Nucleotides ; Open reading frames ; Peptides ; Physiological effects ; Proteins ; Spermatids ; Spermatids - physiology ; Spermatogenesis ; Spermatogenesis - genetics ; Spermatogenesis - physiology ; Testes ; Testis - cytology ; Transcription activation ; Transcription factors</subject><ispartof>Endocrinology (Philadelphia), 2002-05, Vol.143 (5), p.1578-1588</ispartof><rights>Copyright © 2002 by The Endocrine Society 2002</rights><rights>Copyright © 2002 by The Endocrine Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4252-4136c5d85258ba69a4ad82ec6276f7b7cc423d26d8fbc256bf58ca4de9d8213e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11956138$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stelzer, Gil</creatorcontrib><creatorcontrib>Don, Jeremy</creatorcontrib><title>Atce1: A Novel Mouse Cyclic Adenosine 3′,5′-Monophosphate-Responsive Element-Binding Protein-Like Gene Exclusively Expressed in Postmeiotic Spermatids</title><title>Endocrinology (Philadelphia)</title><addtitle>Endocrinology</addtitle><description>Members of the ATF/CREB family of transcription factors are involved in gene activation in various physiological systems ranging from metabolite homeostasis, through regulation of cell cycle, to learning and memory. Two members of this family, cAMP-responsive element binding protein (CREB), and cAMP-responsive element modulator (CREM) are active during mammalian spermatogenesis and are required for this process, as has been shown by knockout and dominant negative experiments. In an effort to identify mouse proteins that interact with the testis-specific protein Tctex2, a mouse testis expression library was screened via the two-hybrid system, using the carboxyl-terminal portion of this protein as bait. A clone containing two overlapping open reading frames, related by a frameshift of one nucleotide, was subsequently isolated. The peptide that interacted with Tctex2 does not initiate from a consensus AUG codon, and it is not clear whether it exists physiologically. However, the other reading frame, initiating from an AUG codon, encodes a 315-amino acid peptide with significant sequence homology to a subfamily of the CREB genes whose prototype is the mouse LZIP peptide. This novel CREB-like peptide, designated Atce1, is specifically expressed in the testis. A developmental study using Northern hybridization and in situ hybridization analyses revealed that Atce1 transcripts begin to accumulate in testes of mice 24 d after birth, reflecting expression in mid/late round spermatids. Interestingly, EMSAs revealed that in vitro translated Atce1 binds specifically to a nuclear factor-κB-binding element rather then to a CRE element. Potential roles for Atce1 are discussed.</description><subject>Adenosine</subject><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>AMP</subject><subject>Animals</subject><subject>Baits</subject><subject>Base Sequence</subject><subject>Blotting, Northern</subject><subject>Cell activation</subject><subject>Cell cycle</subject><subject>Chromosome Mapping</subject><subject>Conserved sequence</subject><subject>Cyclic AMP response element modulator</subject><subject>Cyclic AMP response element-binding protein</subject><subject>Cyclic AMP Response Element-Binding Protein - genetics</subject><subject>DNA - metabolism</subject><subject>Electrophoresis</subject><subject>Gene Library</subject><subject>Gene regulation</subject><subject>Haploidy</subject><subject>Homeostasis</subject><subject>Homology</subject><subject>Hybrid Cells</subject><subject>Hybrid systems</subject><subject>Hybridization</subject><subject>In Situ Hybridization</subject><subject>Male</subject><subject>Meiosis - genetics</subject><subject>Metabolites</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Nucleotides</subject><subject>Open reading frames</subject><subject>Peptides</subject><subject>Physiological effects</subject><subject>Proteins</subject><subject>Spermatids</subject><subject>Spermatids - physiology</subject><subject>Spermatogenesis</subject><subject>Spermatogenesis - genetics</subject><subject>Spermatogenesis - physiology</subject><subject>Testes</subject><subject>Testis - cytology</subject><subject>Transcription activation</subject><subject>Transcription factors</subject><issn>0013-7227</issn><issn>1945-7170</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkctu1DAUhi0EotPCmh2yhMQCkWl8TdLdMBoK0hQqLmsrsU-oS2IHO6k6O56Dx-CReBIcZqRKSEhsfJG_8_vofAg9IfmSUJKfgjN-SThbimVZUnoPLUjFRVaQIr-PFnlOWFZQWhyh4xiv05Vzzh6iI0IqIQkrF-jHatRAzvAKv_M30OELP0XA653urMYrA85H6wCzX99_vhRpyS6888OVj8NVPUL2AeLgXbQ3gDcd9ODG7JV1xrov-DL4EazLtvYr4HNIIZtb3U0z2-3SeQgQIxhsHb70cezB-jH9-XGA0NejNfERetDWXYTHh_0EfX69-bR-k23fn79dr7aZ5lTQjBMmtTCloKJsalnVvDYlBS1pIduiKXTCmKHSlG2jqZBNK0pdcwNVwggDdoKe73OH4L9NEEfV26ih62oHaRqqIDKXZcUT-Owv8NpPwaXeFCMsF4wKkifqdE_p4GMM0Koh2L4OO0VyNUtTszSVpCmhZmmp4ukhd2p6MHf8wVICXuwBPw3_kSb38PygQ7L3Z9J3vf6r8DfdA7Yn</recordid><startdate>200205</startdate><enddate>200205</enddate><creator>Stelzer, Gil</creator><creator>Don, Jeremy</creator><general>Endocrine Society</general><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200205</creationdate><title>Atce1: A Novel Mouse Cyclic Adenosine 3′,5′-Monophosphate-Responsive Element-Binding Protein-Like Gene Exclusively Expressed in Postmeiotic Spermatids</title><author>Stelzer, Gil ; Don, Jeremy</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4252-4136c5d85258ba69a4ad82ec6276f7b7cc423d26d8fbc256bf58ca4de9d8213e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adenosine</topic><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>AMP</topic><topic>Animals</topic><topic>Baits</topic><topic>Base Sequence</topic><topic>Blotting, Northern</topic><topic>Cell activation</topic><topic>Cell cycle</topic><topic>Chromosome Mapping</topic><topic>Conserved sequence</topic><topic>Cyclic AMP response element modulator</topic><topic>Cyclic AMP response element-binding protein</topic><topic>Cyclic AMP Response Element-Binding Protein - genetics</topic><topic>DNA - metabolism</topic><topic>Electrophoresis</topic><topic>Gene Library</topic><topic>Gene regulation</topic><topic>Haploidy</topic><topic>Homeostasis</topic><topic>Homology</topic><topic>Hybrid Cells</topic><topic>Hybrid systems</topic><topic>Hybridization</topic><topic>In Situ Hybridization</topic><topic>Male</topic><topic>Meiosis - genetics</topic><topic>Metabolites</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Nucleotides</topic><topic>Open reading frames</topic><topic>Peptides</topic><topic>Physiological effects</topic><topic>Proteins</topic><topic>Spermatids</topic><topic>Spermatids - physiology</topic><topic>Spermatogenesis</topic><topic>Spermatogenesis - genetics</topic><topic>Spermatogenesis - physiology</topic><topic>Testes</topic><topic>Testis - cytology</topic><topic>Transcription activation</topic><topic>Transcription factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stelzer, Gil</creatorcontrib><creatorcontrib>Don, Jeremy</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Endocrinology (Philadelphia)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stelzer, Gil</au><au>Don, Jeremy</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Atce1: A Novel Mouse Cyclic Adenosine 3′,5′-Monophosphate-Responsive Element-Binding Protein-Like Gene Exclusively Expressed in Postmeiotic Spermatids</atitle><jtitle>Endocrinology (Philadelphia)</jtitle><addtitle>Endocrinology</addtitle><date>2002-05</date><risdate>2002</risdate><volume>143</volume><issue>5</issue><spage>1578</spage><epage>1588</epage><pages>1578-1588</pages><issn>0013-7227</issn><eissn>1945-7170</eissn><abstract>Members of the ATF/CREB family of transcription factors are involved in gene activation in various physiological systems ranging from metabolite homeostasis, through regulation of cell cycle, to learning and memory. Two members of this family, cAMP-responsive element binding protein (CREB), and cAMP-responsive element modulator (CREM) are active during mammalian spermatogenesis and are required for this process, as has been shown by knockout and dominant negative experiments. In an effort to identify mouse proteins that interact with the testis-specific protein Tctex2, a mouse testis expression library was screened via the two-hybrid system, using the carboxyl-terminal portion of this protein as bait. A clone containing two overlapping open reading frames, related by a frameshift of one nucleotide, was subsequently isolated. The peptide that interacted with Tctex2 does not initiate from a consensus AUG codon, and it is not clear whether it exists physiologically. However, the other reading frame, initiating from an AUG codon, encodes a 315-amino acid peptide with significant sequence homology to a subfamily of the CREB genes whose prototype is the mouse LZIP peptide. This novel CREB-like peptide, designated Atce1, is specifically expressed in the testis. A developmental study using Northern hybridization and in situ hybridization analyses revealed that Atce1 transcripts begin to accumulate in testes of mice 24 d after birth, reflecting expression in mid/late round spermatids. Interestingly, EMSAs revealed that in vitro translated Atce1 binds specifically to a nuclear factor-κB-binding element rather then to a CRE element. Potential roles for Atce1 are discussed.</abstract><cop>United States</cop><pub>Endocrine Society</pub><pmid>11956138</pmid><doi>10.1210/endo.143.5.8822</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Adenosine Amino Acid Sequence Amino acids AMP Animals Baits Base Sequence Blotting, Northern Cell activation Cell cycle Chromosome Mapping Conserved sequence Cyclic AMP response element modulator Cyclic AMP response element-binding protein Cyclic AMP Response Element-Binding Protein - genetics DNA - metabolism Electrophoresis Gene Library Gene regulation Haploidy Homeostasis Homology Hybrid Cells Hybrid systems Hybridization In Situ Hybridization Male Meiosis - genetics Metabolites Mice Molecular Sequence Data Nucleotides Open reading frames Peptides Physiological effects Proteins Spermatids Spermatids - physiology Spermatogenesis Spermatogenesis - genetics Spermatogenesis - physiology Testes Testis - cytology Transcription activation Transcription factors |
title | Atce1: A Novel Mouse Cyclic Adenosine 3′,5′-Monophosphate-Responsive Element-Binding Protein-Like Gene Exclusively Expressed in Postmeiotic Spermatids |
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