Testosterone inhibits bradykinin-induced intracellular calcium kinetics in rat aortic endothelial cells in culture
Sex steroids have been associated with cardiovascular diseases and the modification of the risk of coronary artery disease (CAD). We cultured aortic endothelial cells from young adult male rats and loaded them with Fura 2 in order to evaluate the direct effects of testosterone on endothelial cells a...
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| Veröffentlicht in: | Steroids 2002-04, Vol.67 (5), p.393-397 |
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| creator | Rubio-Gayosso, Ivan Garcia-Ramirez, Olga Gutierrez-Serdan, Ruth Guevara-Balcazar, Gustavo Muñoz-Garcı́a, Olga Morato-Cartajena, Tomas Zamora-Garza, Miguel Ceballos-Reyes, Guillermo |
| description | Sex steroids have been associated with cardiovascular diseases and the modification of the risk of coronary artery disease (CAD). We cultured aortic endothelial cells from young adult male rats and loaded them with Fura 2 in order to evaluate the direct effects of testosterone on endothelial cells and the probable regulation of bradykinin-induced effects on intracellular calcium ([Ca
2+]
i) kinetics, effects that are mediated through an increase in intracellular [IP
3], which in turn stimulates the rapid release of Ca
2+ from ER stores. Our results show that testosterone had no direct effects on [Ca
2+]
i kinetics, but did block bradykinin-induced increases in intracellular calcium concentration in endothelial cells. This effect was concentration-dependent; the steroid was applied only 30 s before bradykinin application and thus, the effect can be considered nongenomic in origin. Membrane localization of a putative androgen receptor in endothelial cells could be responsible for this effect. In summary, testosterone can modulate the effects induced by activation of membrane-bound bradykinin receptors. |
| doi_str_mv | 10.1016/S0039-128X(01)00192-1 |
| format | Article |
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2+]
i) kinetics, effects that are mediated through an increase in intracellular [IP
3], which in turn stimulates the rapid release of Ca
2+ from ER stores. Our results show that testosterone had no direct effects on [Ca
2+]
i kinetics, but did block bradykinin-induced increases in intracellular calcium concentration in endothelial cells. This effect was concentration-dependent; the steroid was applied only 30 s before bradykinin application and thus, the effect can be considered nongenomic in origin. Membrane localization of a putative androgen receptor in endothelial cells could be responsible for this effect. In summary, testosterone can modulate the effects induced by activation of membrane-bound bradykinin receptors.</description><identifier>ISSN: 0039-128X</identifier><identifier>EISSN: 1878-5867</identifier><identifier>DOI: 10.1016/S0039-128X(01)00192-1</identifier><identifier>PMID: 11958796</identifier><identifier>CODEN: STEDAM</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Animals ; Aorta - drug effects ; Aorta - metabolism ; Aorta, Thoracic ; Biological and medical sciences ; Bradykinin ; Bradykinin - antagonists & inhibitors ; Bradykinin - pharmacology ; Calcium - metabolism ; Calcium-agonist ; Cells, Cultured ; Endothelium ; Endothelium, Vascular - drug effects ; Endothelium, Vascular - metabolism ; Gonadal Steroid Hormones - pharmacology ; Immunoenzyme Techniques ; Kinetics ; Male ; Microscopy, Confocal ; Nongenomic ; Rats ; Receptors, Androgen - immunology ; Receptors, Androgen - metabolism ; Receptors, Bradykinin - metabolism ; Steroid ; Testosterone ; Testosterone - pharmacology</subject><ispartof>Steroids, 2002-04, Vol.67 (5), p.393-397</ispartof><rights>2002 Elsevier Science Inc.</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-991121d6d89ae108df886f87f1acb840c953d9100e5852aa243d8aac5fd673e63</citedby><cites>FETCH-LOGICAL-c391t-991121d6d89ae108df886f87f1acb840c953d9100e5852aa243d8aac5fd673e63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0039-128X(01)00192-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13538476$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11958796$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rubio-Gayosso, Ivan</creatorcontrib><creatorcontrib>Garcia-Ramirez, Olga</creatorcontrib><creatorcontrib>Gutierrez-Serdan, Ruth</creatorcontrib><creatorcontrib>Guevara-Balcazar, Gustavo</creatorcontrib><creatorcontrib>Muñoz-Garcı́a, Olga</creatorcontrib><creatorcontrib>Morato-Cartajena, Tomas</creatorcontrib><creatorcontrib>Zamora-Garza, Miguel</creatorcontrib><creatorcontrib>Ceballos-Reyes, Guillermo</creatorcontrib><title>Testosterone inhibits bradykinin-induced intracellular calcium kinetics in rat aortic endothelial cells in culture</title><title>Steroids</title><addtitle>Steroids</addtitle><description>Sex steroids have been associated with cardiovascular diseases and the modification of the risk of coronary artery disease (CAD). We cultured aortic endothelial cells from young adult male rats and loaded them with Fura 2 in order to evaluate the direct effects of testosterone on endothelial cells and the probable regulation of bradykinin-induced effects on intracellular calcium ([Ca
2+]
i) kinetics, effects that are mediated through an increase in intracellular [IP
3], which in turn stimulates the rapid release of Ca
2+ from ER stores. Our results show that testosterone had no direct effects on [Ca
2+]
i kinetics, but did block bradykinin-induced increases in intracellular calcium concentration in endothelial cells. This effect was concentration-dependent; the steroid was applied only 30 s before bradykinin application and thus, the effect can be considered nongenomic in origin. Membrane localization of a putative androgen receptor in endothelial cells could be responsible for this effect. In summary, testosterone can modulate the effects induced by activation of membrane-bound bradykinin receptors.</description><subject>Animals</subject><subject>Aorta - drug effects</subject><subject>Aorta - metabolism</subject><subject>Aorta, Thoracic</subject><subject>Biological and medical sciences</subject><subject>Bradykinin</subject><subject>Bradykinin - antagonists & inhibitors</subject><subject>Bradykinin - pharmacology</subject><subject>Calcium - metabolism</subject><subject>Calcium-agonist</subject><subject>Cells, Cultured</subject><subject>Endothelium</subject><subject>Endothelium, Vascular - drug effects</subject><subject>Endothelium, Vascular - metabolism</subject><subject>Gonadal Steroid Hormones - pharmacology</subject><subject>Immunoenzyme Techniques</subject><subject>Kinetics</subject><subject>Male</subject><subject>Microscopy, Confocal</subject><subject>Nongenomic</subject><subject>Rats</subject><subject>Receptors, Androgen - immunology</subject><subject>Receptors, Androgen - metabolism</subject><subject>Receptors, Bradykinin - metabolism</subject><subject>Steroid</subject><subject>Testosterone</subject><subject>Testosterone - pharmacology</subject><issn>0039-128X</issn><issn>1878-5867</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE2LFDEQhoMo7rj6E5S-KHporepMp5PTIotfsODBFbyFmqSajfak1yQt7L83PTO4R08hvM-bVD1CPEd4i4Dq3TcAaVrs9I_XgG8A0HQtPhAb1INue62Gh2LzDzkTT3L-CQBKmu6xOEM0vR6M2oh0zbnMuXCaIzch3oRdKLnZJfJ3v0IMsQ3RL459zUoix9O0TJQaR5MLy76pDJfgco2bRKWhOdVrw9HP5YanQFOzdg65W6ayJH4qHo00ZX52Os_F948fri8_t1dfP325fH_VOmmwtMYgduiV14YYQftRazXqYURyO70FZ3rpDQJwr_uOqNtKr4lcP3o1SFbyXLw6vnub5t9LXdPuQ16Hocjzku2AChR0uoL9EXRpzjnxaG9T2FO6swh2lW0Psu1q0gLag2yLtffi9MGy27O_b53sVuDlCaBchY2Jogv5npO91Nth5S6OHFcdfwInm13gWK2HxK5YP4f_jPIXkzyegQ</recordid><startdate>20020401</startdate><enddate>20020401</enddate><creator>Rubio-Gayosso, Ivan</creator><creator>Garcia-Ramirez, Olga</creator><creator>Gutierrez-Serdan, Ruth</creator><creator>Guevara-Balcazar, Gustavo</creator><creator>Muñoz-Garcı́a, Olga</creator><creator>Morato-Cartajena, Tomas</creator><creator>Zamora-Garza, Miguel</creator><creator>Ceballos-Reyes, Guillermo</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020401</creationdate><title>Testosterone inhibits bradykinin-induced intracellular calcium kinetics in rat aortic endothelial cells in culture</title><author>Rubio-Gayosso, Ivan ; Garcia-Ramirez, Olga ; Gutierrez-Serdan, Ruth ; Guevara-Balcazar, Gustavo ; Muñoz-Garcı́a, Olga ; Morato-Cartajena, Tomas ; Zamora-Garza, Miguel ; Ceballos-Reyes, Guillermo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-991121d6d89ae108df886f87f1acb840c953d9100e5852aa243d8aac5fd673e63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Aorta - drug effects</topic><topic>Aorta - metabolism</topic><topic>Aorta, Thoracic</topic><topic>Biological and medical sciences</topic><topic>Bradykinin</topic><topic>Bradykinin - antagonists & inhibitors</topic><topic>Bradykinin - pharmacology</topic><topic>Calcium - metabolism</topic><topic>Calcium-agonist</topic><topic>Cells, Cultured</topic><topic>Endothelium</topic><topic>Endothelium, Vascular - drug effects</topic><topic>Endothelium, Vascular - metabolism</topic><topic>Gonadal Steroid Hormones - pharmacology</topic><topic>Immunoenzyme Techniques</topic><topic>Kinetics</topic><topic>Male</topic><topic>Microscopy, Confocal</topic><topic>Nongenomic</topic><topic>Rats</topic><topic>Receptors, Androgen - immunology</topic><topic>Receptors, Androgen - metabolism</topic><topic>Receptors, Bradykinin - metabolism</topic><topic>Steroid</topic><topic>Testosterone</topic><topic>Testosterone - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rubio-Gayosso, Ivan</creatorcontrib><creatorcontrib>Garcia-Ramirez, Olga</creatorcontrib><creatorcontrib>Gutierrez-Serdan, Ruth</creatorcontrib><creatorcontrib>Guevara-Balcazar, Gustavo</creatorcontrib><creatorcontrib>Muñoz-Garcı́a, Olga</creatorcontrib><creatorcontrib>Morato-Cartajena, Tomas</creatorcontrib><creatorcontrib>Zamora-Garza, Miguel</creatorcontrib><creatorcontrib>Ceballos-Reyes, Guillermo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Steroids</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rubio-Gayosso, Ivan</au><au>Garcia-Ramirez, Olga</au><au>Gutierrez-Serdan, Ruth</au><au>Guevara-Balcazar, Gustavo</au><au>Muñoz-Garcı́a, Olga</au><au>Morato-Cartajena, Tomas</au><au>Zamora-Garza, Miguel</au><au>Ceballos-Reyes, Guillermo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Testosterone inhibits bradykinin-induced intracellular calcium kinetics in rat aortic endothelial cells in culture</atitle><jtitle>Steroids</jtitle><addtitle>Steroids</addtitle><date>2002-04-01</date><risdate>2002</risdate><volume>67</volume><issue>5</issue><spage>393</spage><epage>397</epage><pages>393-397</pages><issn>0039-128X</issn><eissn>1878-5867</eissn><coden>STEDAM</coden><abstract>Sex steroids have been associated with cardiovascular diseases and the modification of the risk of coronary artery disease (CAD). We cultured aortic endothelial cells from young adult male rats and loaded them with Fura 2 in order to evaluate the direct effects of testosterone on endothelial cells and the probable regulation of bradykinin-induced effects on intracellular calcium ([Ca
2+]
i) kinetics, effects that are mediated through an increase in intracellular [IP
3], which in turn stimulates the rapid release of Ca
2+ from ER stores. Our results show that testosterone had no direct effects on [Ca
2+]
i kinetics, but did block bradykinin-induced increases in intracellular calcium concentration in endothelial cells. This effect was concentration-dependent; the steroid was applied only 30 s before bradykinin application and thus, the effect can be considered nongenomic in origin. Membrane localization of a putative androgen receptor in endothelial cells could be responsible for this effect. In summary, testosterone can modulate the effects induced by activation of membrane-bound bradykinin receptors.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>11958796</pmid><doi>10.1016/S0039-128X(01)00192-1</doi><tpages>5</tpages></addata></record> |
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| subjects | Animals Aorta - drug effects Aorta - metabolism Aorta, Thoracic Biological and medical sciences Bradykinin Bradykinin - antagonists & inhibitors Bradykinin - pharmacology Calcium - metabolism Calcium-agonist Cells, Cultured Endothelium Endothelium, Vascular - drug effects Endothelium, Vascular - metabolism Gonadal Steroid Hormones - pharmacology Immunoenzyme Techniques Kinetics Male Microscopy, Confocal Nongenomic Rats Receptors, Androgen - immunology Receptors, Androgen - metabolism Receptors, Bradykinin - metabolism Steroid Testosterone Testosterone - pharmacology |
| title | Testosterone inhibits bradykinin-induced intracellular calcium kinetics in rat aortic endothelial cells in culture |
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