Identification of a novel protein p59(scr), which is expressed at specific stages of mouse spermatogenesis
A novel cDNA-encoding polypeptide of 545 amino acid residues was identified from a mouse testis cDNA library. The transcript of this gene, p59(scr), was predominantly expressed in the testis and was developmentally regulated during spermatogenesis. The encoded protein was expressed in spermatocytes...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2002-04, Vol.292 (4), p.992-998 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Senoo, Makoto Hoshino, Shigetaka Mochida, Naoko Matsumura, Yasuko Habu, Sonoko |
| description | A novel cDNA-encoding polypeptide of 545 amino acid residues was identified from a mouse testis cDNA library. The transcript of this gene, p59(scr), was predominantly expressed in the testis and was developmentally regulated during spermatogenesis. The encoded protein was expressed in spermatocytes and round spermatids within seminiferous tubules of the adult testis. Using an adult-mouse model of experimental unilateral cryptorchidism, it was observed that the expression of the p59(scr) mRNA was reduced in the cryptorchid testes in association with destruction of spermatogenesis. In vitro heat stress experiment further demonstrated that p59(scr) mRNA was more sensitive to heat stress than the other mRNA species, such as germ-cell-specific meiosis-activating kinase (mak) gene and a housekeeping beta-actin gene. Our results suggest that this novel p59(scr) gene is involved in spermatogenesis and may play an important role in development of testicular germ cells. |
| doi_str_mv | 10.1006/bbrc.2002.6769 |
| format | Article |
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The transcript of this gene, p59(scr), was predominantly expressed in the testis and was developmentally regulated during spermatogenesis. The encoded protein was expressed in spermatocytes and round spermatids within seminiferous tubules of the adult testis. Using an adult-mouse model of experimental unilateral cryptorchidism, it was observed that the expression of the p59(scr) mRNA was reduced in the cryptorchid testes in association with destruction of spermatogenesis. In vitro heat stress experiment further demonstrated that p59(scr) mRNA was more sensitive to heat stress than the other mRNA species, such as germ-cell-specific meiosis-activating kinase (mak) gene and a housekeeping beta-actin gene. Our results suggest that this novel p59(scr) gene is involved in spermatogenesis and may play an important role in development of testicular germ cells.</description><identifier>ISSN: 0006-291X</identifier><identifier>DOI: 10.1006/bbrc.2002.6769</identifier><identifier>PMID: 11944913</identifier><language>eng</language><publisher>United States</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Cloning, Molecular ; COS Cells ; Cryptorchidism - metabolism ; Cryptorchidism - pathology ; Disease Models, Animal ; Down-Regulation ; Gene Expression Regulation, Developmental ; Gene Library ; Germ Cells - cytology ; Germ Cells - metabolism ; Hot Temperature ; In Situ Hybridization ; Male ; Mice ; Molecular Sequence Data ; Organ Specificity ; Protein Kinases ; Protein-Serine-Threonine Kinases - metabolism ; Proteins - genetics ; Proteins - metabolism ; RNA, Messenger - metabolism ; Spermatids - cytology ; Spermatids - metabolism ; Spermatocytes - cytology ; Spermatocytes - metabolism ; Spermatogenesis - physiology ; Testis - cytology ; Testis - metabolism ; Testis - pathology</subject><ispartof>Biochemical and biophysical research communications, 2002-04, Vol.292 (4), p.992-998</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11944913$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Senoo, Makoto</creatorcontrib><creatorcontrib>Hoshino, Shigetaka</creatorcontrib><creatorcontrib>Mochida, Naoko</creatorcontrib><creatorcontrib>Matsumura, Yasuko</creatorcontrib><creatorcontrib>Habu, Sonoko</creatorcontrib><title>Identification of a novel protein p59(scr), which is expressed at specific stages of mouse spermatogenesis</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>A novel cDNA-encoding polypeptide of 545 amino acid residues was identified from a mouse testis cDNA library. The transcript of this gene, p59(scr), was predominantly expressed in the testis and was developmentally regulated during spermatogenesis. The encoded protein was expressed in spermatocytes and round spermatids within seminiferous tubules of the adult testis. Using an adult-mouse model of experimental unilateral cryptorchidism, it was observed that the expression of the p59(scr) mRNA was reduced in the cryptorchid testes in association with destruction of spermatogenesis. In vitro heat stress experiment further demonstrated that p59(scr) mRNA was more sensitive to heat stress than the other mRNA species, such as germ-cell-specific meiosis-activating kinase (mak) gene and a housekeeping beta-actin gene. Our results suggest that this novel p59(scr) gene is involved in spermatogenesis and may play an important role in development of testicular germ cells.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Cloning, Molecular</subject><subject>COS Cells</subject><subject>Cryptorchidism - metabolism</subject><subject>Cryptorchidism - pathology</subject><subject>Disease Models, Animal</subject><subject>Down-Regulation</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Gene Library</subject><subject>Germ Cells - cytology</subject><subject>Germ Cells - metabolism</subject><subject>Hot Temperature</subject><subject>In Situ Hybridization</subject><subject>Male</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Organ Specificity</subject><subject>Protein Kinases</subject><subject>Protein-Serine-Threonine Kinases - metabolism</subject><subject>Proteins - genetics</subject><subject>Proteins - metabolism</subject><subject>RNA, Messenger - metabolism</subject><subject>Spermatids - cytology</subject><subject>Spermatids - metabolism</subject><subject>Spermatocytes - cytology</subject><subject>Spermatocytes - metabolism</subject><subject>Spermatogenesis - physiology</subject><subject>Testis - cytology</subject><subject>Testis - metabolism</subject><subject>Testis - pathology</subject><issn>0006-291X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kMtLAzEQh3NQbK1ePUpOouDWJLubx1GKj0LBi4K3JcnOtin7cif18d-7xXoamPn4-P2GkAvO5pwxeefc4OeCMTGXSpojMmXjNhGGv0_IKeKWMc4zaU7IhHOTZYanU7JdltDGUAVvY-ha2lXU0rb7hJr2QxchtLTPzTX64eaWfm2C39CAFL77ARChpDZS7MHvBRSjXQPuFU23Q9gfhsbGbg0tYMAzclzZGuH8MGfk7fHhdfGcrF6elov7VdILpmJiHORGWMaMKrXLlJVMpaKqRCatAqnKynDhMuuNMCzXXEPOU6mVdVa5cmRn5OrPOxb42AHGognooa5tC2OuQvHcaJ3qEbw8gDvXQFn0Q2js8FP8fyf9BQYsZZ0</recordid><startdate>20020412</startdate><enddate>20020412</enddate><creator>Senoo, Makoto</creator><creator>Hoshino, Shigetaka</creator><creator>Mochida, Naoko</creator><creator>Matsumura, Yasuko</creator><creator>Habu, Sonoko</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20020412</creationdate><title>Identification of a novel protein p59(scr), which is expressed at specific stages of mouse spermatogenesis</title><author>Senoo, Makoto ; Hoshino, Shigetaka ; Mochida, Naoko ; Matsumura, Yasuko ; Habu, Sonoko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p207t-9be592a0097d8b47a60732ff246a7e67df912b4ac92905818e513687aba7bd073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Cloning, Molecular</topic><topic>COS Cells</topic><topic>Cryptorchidism - metabolism</topic><topic>Cryptorchidism - pathology</topic><topic>Disease Models, Animal</topic><topic>Down-Regulation</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Gene Library</topic><topic>Germ Cells - cytology</topic><topic>Germ Cells - metabolism</topic><topic>Hot Temperature</topic><topic>In Situ Hybridization</topic><topic>Male</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Organ Specificity</topic><topic>Protein Kinases</topic><topic>Protein-Serine-Threonine Kinases - metabolism</topic><topic>Proteins - genetics</topic><topic>Proteins - metabolism</topic><topic>RNA, Messenger - metabolism</topic><topic>Spermatids - cytology</topic><topic>Spermatids - metabolism</topic><topic>Spermatocytes - cytology</topic><topic>Spermatocytes - metabolism</topic><topic>Spermatogenesis - physiology</topic><topic>Testis - cytology</topic><topic>Testis - metabolism</topic><topic>Testis - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Senoo, Makoto</creatorcontrib><creatorcontrib>Hoshino, Shigetaka</creatorcontrib><creatorcontrib>Mochida, Naoko</creatorcontrib><creatorcontrib>Matsumura, Yasuko</creatorcontrib><creatorcontrib>Habu, Sonoko</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Senoo, Makoto</au><au>Hoshino, Shigetaka</au><au>Mochida, Naoko</au><au>Matsumura, Yasuko</au><au>Habu, Sonoko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of a novel protein p59(scr), which is expressed at specific stages of mouse spermatogenesis</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2002-04-12</date><risdate>2002</risdate><volume>292</volume><issue>4</issue><spage>992</spage><epage>998</epage><pages>992-998</pages><issn>0006-291X</issn><abstract>A novel cDNA-encoding polypeptide of 545 amino acid residues was identified from a mouse testis cDNA library. The transcript of this gene, p59(scr), was predominantly expressed in the testis and was developmentally regulated during spermatogenesis. The encoded protein was expressed in spermatocytes and round spermatids within seminiferous tubules of the adult testis. Using an adult-mouse model of experimental unilateral cryptorchidism, it was observed that the expression of the p59(scr) mRNA was reduced in the cryptorchid testes in association with destruction of spermatogenesis. In vitro heat stress experiment further demonstrated that p59(scr) mRNA was more sensitive to heat stress than the other mRNA species, such as germ-cell-specific meiosis-activating kinase (mak) gene and a housekeeping beta-actin gene. Our results suggest that this novel p59(scr) gene is involved in spermatogenesis and may play an important role in development of testicular germ cells.</abstract><cop>United States</cop><pmid>11944913</pmid><doi>10.1006/bbrc.2002.6769</doi><tpages>7</tpages></addata></record> |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence Animals Base Sequence Cloning, Molecular COS Cells Cryptorchidism - metabolism Cryptorchidism - pathology Disease Models, Animal Down-Regulation Gene Expression Regulation, Developmental Gene Library Germ Cells - cytology Germ Cells - metabolism Hot Temperature In Situ Hybridization Male Mice Molecular Sequence Data Organ Specificity Protein Kinases Protein-Serine-Threonine Kinases - metabolism Proteins - genetics Proteins - metabolism RNA, Messenger - metabolism Spermatids - cytology Spermatids - metabolism Spermatocytes - cytology Spermatocytes - metabolism Spermatogenesis - physiology Testis - cytology Testis - metabolism Testis - pathology |
| title | Identification of a novel protein p59(scr), which is expressed at specific stages of mouse spermatogenesis |
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