Cholecystokinin octapeptide inhibits the in vitro expression of CD14 in rat pulmonary interstitial macrophages induced by lipopolysaccharide
To study the effect of cholecystokinin octapeptide (CCK-8) on lipopolysaccharide (LPS)-stimulated pulmonary interstitial macrophages (PIM) in vitro. PIM were isolated and cultured in the presence or absence of LPS, CCK-8, proglumide (the antagonist of CCK receptors) and vehicle. The expression of me...
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| Veröffentlicht in: | Chinese medical journal 2002-02, Vol.115 (2), p.276-279 |
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| creator | Li, Shujin Cong, Bin Yan, Yunli Yao, Yuxia Ma, Chunling Ling, Yiling |
| description | To study the effect of cholecystokinin octapeptide (CCK-8) on lipopolysaccharide (LPS)-stimulated pulmonary interstitial macrophages (PIM) in vitro.
PIM were isolated and cultured in the presence or absence of LPS, CCK-8, proglumide (the antagonist of CCK receptors) and vehicle. The expression of membrane CD14 (mCD14) protein was assayed by flow cytometry and soluble CD14 (sCD14) in the supernatant was analyzed semi-quantitatively by Western blot. TNF-alpha in the supernatant was detected with ELISA.
CCK-8, at concentrations of 10(-7) mol/L and 10(-6) mol/L, significantly inhibited the expression of mCD14. Release of sCD14 and TNF-alpha in the supernatant was up-regulated by LPS (1 microg/ml) but reduced by CCK-8. The effect of CCK-8 was inhibited by proglumide.
CCK-8 negatively modulated several functions of LPS-stimulated PIM through CCK receptors. This may be one of the mechanisms for CCK-8 to alleviate inflammation in lung tissue during endotoxemia. |
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PIM were isolated and cultured in the presence or absence of LPS, CCK-8, proglumide (the antagonist of CCK receptors) and vehicle. The expression of membrane CD14 (mCD14) protein was assayed by flow cytometry and soluble CD14 (sCD14) in the supernatant was analyzed semi-quantitatively by Western blot. TNF-alpha in the supernatant was detected with ELISA.
CCK-8, at concentrations of 10(-7) mol/L and 10(-6) mol/L, significantly inhibited the expression of mCD14. Release of sCD14 and TNF-alpha in the supernatant was up-regulated by LPS (1 microg/ml) but reduced by CCK-8. The effect of CCK-8 was inhibited by proglumide.
CCK-8 negatively modulated several functions of LPS-stimulated PIM through CCK receptors. This may be one of the mechanisms for CCK-8 to alleviate inflammation in lung tissue during endotoxemia.</description><identifier>ISSN: 0366-6999</identifier><identifier>PMID: 11940348</identifier><language>eng</language><publisher>China</publisher><subject>Animals ; Cells, Cultured ; Culture Media, Conditioned - chemistry ; Female ; Lipopolysaccharide Receptors - biosynthesis ; Lipopolysaccharides - pharmacology ; Macrophages, Alveolar - cytology ; Macrophages, Alveolar - drug effects ; Macrophages, Alveolar - metabolism ; Rats ; Rats, Sprague-Dawley ; Sincalide - pharmacology ; Specific Pathogen-Free Organisms ; Tumor Necrosis Factor-alpha - drug effects ; Tumor Necrosis Factor-alpha - secretion</subject><ispartof>Chinese medical journal, 2002-02, Vol.115 (2), p.276-279</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11940348$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Shujin</creatorcontrib><creatorcontrib>Cong, Bin</creatorcontrib><creatorcontrib>Yan, Yunli</creatorcontrib><creatorcontrib>Yao, Yuxia</creatorcontrib><creatorcontrib>Ma, Chunling</creatorcontrib><creatorcontrib>Ling, Yiling</creatorcontrib><title>Cholecystokinin octapeptide inhibits the in vitro expression of CD14 in rat pulmonary interstitial macrophages induced by lipopolysaccharide</title><title>Chinese medical journal</title><addtitle>Chin Med J (Engl)</addtitle><description>To study the effect of cholecystokinin octapeptide (CCK-8) on lipopolysaccharide (LPS)-stimulated pulmonary interstitial macrophages (PIM) in vitro.
PIM were isolated and cultured in the presence or absence of LPS, CCK-8, proglumide (the antagonist of CCK receptors) and vehicle. The expression of membrane CD14 (mCD14) protein was assayed by flow cytometry and soluble CD14 (sCD14) in the supernatant was analyzed semi-quantitatively by Western blot. TNF-alpha in the supernatant was detected with ELISA.
CCK-8, at concentrations of 10(-7) mol/L and 10(-6) mol/L, significantly inhibited the expression of mCD14. Release of sCD14 and TNF-alpha in the supernatant was up-regulated by LPS (1 microg/ml) but reduced by CCK-8. The effect of CCK-8 was inhibited by proglumide.
CCK-8 negatively modulated several functions of LPS-stimulated PIM through CCK receptors. This may be one of the mechanisms for CCK-8 to alleviate inflammation in lung tissue during endotoxemia.</description><subject>Animals</subject><subject>Cells, Cultured</subject><subject>Culture Media, Conditioned - chemistry</subject><subject>Female</subject><subject>Lipopolysaccharide Receptors - biosynthesis</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Macrophages, Alveolar - cytology</subject><subject>Macrophages, Alveolar - drug effects</subject><subject>Macrophages, Alveolar - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Sincalide - pharmacology</subject><subject>Specific Pathogen-Free Organisms</subject><subject>Tumor Necrosis Factor-alpha - drug effects</subject><subject>Tumor Necrosis Factor-alpha - secretion</subject><issn>0366-6999</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kMtOwzAQRb0A0VL4BeQVu0hOHCf1EoWnVIkNrCM_psTgxMZ2EPkHPhpXlNVo5hzNaO4JWhPaNEXDOV-h8xjfCakYa5sztCpLXhNab9fopxucBbXE5D7MZCbsVBIefDIasJkGI02KOA2HBn-ZFByGbx8gRuOyvMfdbVkfWBAJ-9mObhJhyYMEISaTjLB4FCo4P4g3iBnoWYHGcsHWeOedXaJQahAhH7xAp3thI1we6wa93t-9dI_F7vnhqbvZFb4ibSp0SyvOt6wlwARUdcUroYHqlkvFAJhUHFQGmpQERNXKhlIt633Da6UZpXSDrv_2-uA-Z4ipH01UYK2YwM2xb0vGWc4ti1dHcZYj6N4HM-b3-v_86C87YG_Y</recordid><startdate>20020201</startdate><enddate>20020201</enddate><creator>Li, Shujin</creator><creator>Cong, Bin</creator><creator>Yan, Yunli</creator><creator>Yao, Yuxia</creator><creator>Ma, Chunling</creator><creator>Ling, Yiling</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20020201</creationdate><title>Cholecystokinin octapeptide inhibits the in vitro expression of CD14 in rat pulmonary interstitial macrophages induced by lipopolysaccharide</title><author>Li, Shujin ; Cong, Bin ; Yan, Yunli ; Yao, Yuxia ; Ma, Chunling ; Ling, Yiling</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p207t-d732998570e5ae24292ade3d79bc5ee5bc9ecae2d010ea27b633db4f694cd5333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Cells, Cultured</topic><topic>Culture Media, Conditioned - chemistry</topic><topic>Female</topic><topic>Lipopolysaccharide Receptors - biosynthesis</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Macrophages, Alveolar - cytology</topic><topic>Macrophages, Alveolar - drug effects</topic><topic>Macrophages, Alveolar - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Sincalide - pharmacology</topic><topic>Specific Pathogen-Free Organisms</topic><topic>Tumor Necrosis Factor-alpha - drug effects</topic><topic>Tumor Necrosis Factor-alpha - secretion</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Shujin</creatorcontrib><creatorcontrib>Cong, Bin</creatorcontrib><creatorcontrib>Yan, Yunli</creatorcontrib><creatorcontrib>Yao, Yuxia</creatorcontrib><creatorcontrib>Ma, Chunling</creatorcontrib><creatorcontrib>Ling, Yiling</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Chinese medical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Shujin</au><au>Cong, Bin</au><au>Yan, Yunli</au><au>Yao, Yuxia</au><au>Ma, Chunling</au><au>Ling, Yiling</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cholecystokinin octapeptide inhibits the in vitro expression of CD14 in rat pulmonary interstitial macrophages induced by lipopolysaccharide</atitle><jtitle>Chinese medical journal</jtitle><addtitle>Chin Med J (Engl)</addtitle><date>2002-02-01</date><risdate>2002</risdate><volume>115</volume><issue>2</issue><spage>276</spage><epage>279</epage><pages>276-279</pages><issn>0366-6999</issn><abstract>To study the effect of cholecystokinin octapeptide (CCK-8) on lipopolysaccharide (LPS)-stimulated pulmonary interstitial macrophages (PIM) in vitro.
PIM were isolated and cultured in the presence or absence of LPS, CCK-8, proglumide (the antagonist of CCK receptors) and vehicle. The expression of membrane CD14 (mCD14) protein was assayed by flow cytometry and soluble CD14 (sCD14) in the supernatant was analyzed semi-quantitatively by Western blot. TNF-alpha in the supernatant was detected with ELISA.
CCK-8, at concentrations of 10(-7) mol/L and 10(-6) mol/L, significantly inhibited the expression of mCD14. Release of sCD14 and TNF-alpha in the supernatant was up-regulated by LPS (1 microg/ml) but reduced by CCK-8. The effect of CCK-8 was inhibited by proglumide.
CCK-8 negatively modulated several functions of LPS-stimulated PIM through CCK receptors. This may be one of the mechanisms for CCK-8 to alleviate inflammation in lung tissue during endotoxemia.</abstract><cop>China</cop><pmid>11940348</pmid><tpages>4</tpages></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Cells, Cultured Culture Media, Conditioned - chemistry Female Lipopolysaccharide Receptors - biosynthesis Lipopolysaccharides - pharmacology Macrophages, Alveolar - cytology Macrophages, Alveolar - drug effects Macrophages, Alveolar - metabolism Rats Rats, Sprague-Dawley Sincalide - pharmacology Specific Pathogen-Free Organisms Tumor Necrosis Factor-alpha - drug effects Tumor Necrosis Factor-alpha - secretion |
| title | Cholecystokinin octapeptide inhibits the in vitro expression of CD14 in rat pulmonary interstitial macrophages induced by lipopolysaccharide |
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