The first isolation of equine arteritis virus in Argentina
This paper describes the first isolation of equine arteritis virus (EAV) in Argentina. The virus was isolated from the semen of an imported seropositive stallion held in isolation at a breeding farm in Tandil in the Buenos Aires Province. In addition, viral nucleic acid was detected in seminal plasm...
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| Veröffentlicht in: | Revue scientifique et technique (International Office of Epizootics) 2003-12, Vol.22 (3), p.1029-1033 |
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| container_title | Revue scientifique et technique (International Office of Epizootics) |
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| creator | Echeverría, M G Pecoraro, M R Galosi, C M Etcheverrigaray, M E Nosetto, E O |
| description | This paper describes the first isolation of equine arteritis virus (EAV) in Argentina. The virus was isolated from the semen of an imported seropositive stallion held in isolation at a breeding farm in Tandil in the Buenos Aires Province. In addition, viral nucleic acid was detected in seminal plasma using the reverse-transcription polymerase chain reaction. The isolated virus was propagated in cell cultures and confirmed as EAV by indirect immunofluorescence and virus neutralisation, using a serum specific for the reference Bucyrus strain of EAV. As far as the authors are aware, this is the first time that EAV has been isolated in South America. The equine industry is very important for Argentina and international movement of horses is very intensive. This finding may have effects on the international trade of horses and semen from Argentina. |
| doi_str_mv | 10.20506/rst.22.3.1458 |
| format | Article |
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The virus was isolated from the semen of an imported seropositive stallion held in isolation at a breeding farm in Tandil in the Buenos Aires Province. In addition, viral nucleic acid was detected in seminal plasma using the reverse-transcription polymerase chain reaction. The isolated virus was propagated in cell cultures and confirmed as EAV by indirect immunofluorescence and virus neutralisation, using a serum specific for the reference Bucyrus strain of EAV. As far as the authors are aware, this is the first time that EAV has been isolated in South America. The equine industry is very important for Argentina and international movement of horses is very intensive. 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The virus was isolated from the semen of an imported seropositive stallion held in isolation at a breeding farm in Tandil in the Buenos Aires Province. In addition, viral nucleic acid was detected in seminal plasma using the reverse-transcription polymerase chain reaction. The isolated virus was propagated in cell cultures and confirmed as EAV by indirect immunofluorescence and virus neutralisation, using a serum specific for the reference Bucyrus strain of EAV. As far as the authors are aware, this is the first time that EAV has been isolated in South America. The equine industry is very important for Argentina and international movement of horses is very intensive. This finding may have effects on the international trade of horses and semen from Argentina.</description><subject>Animals</subject><subject>Antigens, Viral - analysis</subject><subject>Argentina</subject><subject>Arteritis Virus, Equine - genetics</subject><subject>Arteritis Virus, Equine - immunology</subject><subject>Arteritis Virus, Equine - isolation & purification</subject><subject>Arterivirus Infections - veterinary</subject><subject>Arterivirus Infections - virology</subject><subject>Cell Line</subject><subject>Cytopathogenic Effect, Viral</subject><subject>DNA, Complementary - analysis</subject><subject>Fluorescent Antibody Technique - veterinary</subject><subject>Horse Diseases - virology</subject><subject>Horses</subject><subject>Male</subject><subject>Neutralization Tests - veterinary</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - veterinary</subject><subject>RNA, Viral - isolation & purification</subject><subject>Semen - virology</subject><issn>0253-1933</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1jztPwzAURj2AaCmsjMgTW4JfN7HZqoqXVImlzJGTXINR4rS2g8S_pxJlOss5n_QRcsNZKRiw6j6mXApRypIr0GdkyQTIghspF-QypS_GKiO1viALDowBgFmSh90nUuePJfVpGmz2U6CTo3iYfUBqY8bos0_028c5UR_oOn5gyD7YK3Lu7JDw-sQVeX963G1eiu3b8-tmvS06YUQuelDW9i3qDrhqNVdSCeOE7KwWrai5wZ5pW2uJCOhAM6E12E5aZmqJrpUrcve3u4_TYcaUm9GnDofBBpzm1NQcjKqMOYq3J3FuR-ybffSjjT_N_1v5Cz2BVMM</recordid><startdate>20031201</startdate><enddate>20031201</enddate><creator>Echeverría, M G</creator><creator>Pecoraro, M R</creator><creator>Galosi, C M</creator><creator>Etcheverrigaray, M E</creator><creator>Nosetto, E O</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20031201</creationdate><title>The first isolation of equine arteritis virus in Argentina</title><author>Echeverría, M G ; Pecoraro, M R ; Galosi, C M ; Etcheverrigaray, M E ; Nosetto, E O</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c292t-d54aadbe8c514b8143429f23ca82b2719ed08a783ee5ef5802885ac3a0973efb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Antigens, Viral - analysis</topic><topic>Argentina</topic><topic>Arteritis Virus, Equine - genetics</topic><topic>Arteritis Virus, Equine - immunology</topic><topic>Arteritis Virus, Equine - isolation & purification</topic><topic>Arterivirus Infections - veterinary</topic><topic>Arterivirus Infections - virology</topic><topic>Cell Line</topic><topic>Cytopathogenic Effect, Viral</topic><topic>DNA, Complementary - analysis</topic><topic>Fluorescent Antibody Technique - veterinary</topic><topic>Horse Diseases - virology</topic><topic>Horses</topic><topic>Male</topic><topic>Neutralization Tests - veterinary</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - veterinary</topic><topic>RNA, Viral - isolation & purification</topic><topic>Semen - virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Echeverría, M G</creatorcontrib><creatorcontrib>Pecoraro, M R</creatorcontrib><creatorcontrib>Galosi, C M</creatorcontrib><creatorcontrib>Etcheverrigaray, M E</creatorcontrib><creatorcontrib>Nosetto, E O</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Revue scientifique et technique (International Office of Epizootics)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Echeverría, M G</au><au>Pecoraro, M R</au><au>Galosi, C M</au><au>Etcheverrigaray, M E</au><au>Nosetto, E O</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The first isolation of equine arteritis virus in Argentina</atitle><jtitle>Revue scientifique et technique (International Office of Epizootics)</jtitle><addtitle>Rev Sci Tech</addtitle><date>2003-12-01</date><risdate>2003</risdate><volume>22</volume><issue>3</issue><spage>1029</spage><epage>1033</epage><pages>1029-1033</pages><issn>0253-1933</issn><abstract>This paper describes the first isolation of equine arteritis virus (EAV) in Argentina. 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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Antigens, Viral - analysis Argentina Arteritis Virus, Equine - genetics Arteritis Virus, Equine - immunology Arteritis Virus, Equine - isolation & purification Arterivirus Infections - veterinary Arterivirus Infections - virology Cell Line Cytopathogenic Effect, Viral DNA, Complementary - analysis Fluorescent Antibody Technique - veterinary Horse Diseases - virology Horses Male Neutralization Tests - veterinary Reverse Transcriptase Polymerase Chain Reaction - veterinary RNA, Viral - isolation & purification Semen - virology |
| title | The first isolation of equine arteritis virus in Argentina |
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