Polarization immunoassays using reactive ruthenium metal-ligand complexes as luminescent labels

The first competitive fluorescence polarization immunoassay using ruthenium metal-ligand complexes (5-MC and 55-DC) as labels is described. These were newly synthesized and characterized in terms of their spectra, their covalent linkage to proteins, and their use in both homogeneous and competitive...

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Veröffentlicht in:	Analytical and bioanalytical chemistry 2002-03, Vol.372 (5-6), p.688-694
Hauptverfasser:	Dürkop, Axel, Lehmann, Frank, Wolfbeis, Otto S
Format:	Artikel
Sprache:	eng
Schlagworte:	Buffers Chromatography, Gel Chromatography, Thin Layer Complexation Esters Fluorescence Fluorescence Polarization Immunoassay - methods Fluorescent Dyes - chemistry Hydrogen-Ion Concentration Ligands Myoglobin - analysis Polarization Proteins Ruthenium Ruthenium - chemistry Serum Albumin - analysis Synthesis (chemical)
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