Characterization and quantification of metallothionein isoforms by capillary electrophoresis-inductively coupled plasma-isotope-dilution mass spectrometry
In a new approach to the characterization and quantification of metallothionein isoforms an on-line isotope-dilution method in combination with the coupling of capillary electrophoresis (CE) to an inductively coupled plasma-sector field mass spectrometer (ICP-SFMS) is reported. Metallothionein (MT)...
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Veröffentlicht in: | Analytical and bioanalytical chemistry 2002-01, Vol.372 (1), p.155-163 |
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creator | Schaumlöffel, Dirk Prange, Andreas Marx, Gizelher Heumann, Klaus G Brätter, Peter |
description | In a new approach to the characterization and quantification of metallothionein isoforms an on-line isotope-dilution method in combination with the coupling of capillary electrophoresis (CE) to an inductively coupled plasma-sector field mass spectrometer (ICP-SFMS) is reported. Metallothionein (MT) isoforms are separated by CE and the elements Cu, Zn, Cd, and S are detected simultaneously by use of ICP-SFMS in the medium resolution mode. On-line isotope dilution is performed by continuous introduction of an isotopically enriched, species-unspecific spike solution after the separation step. MT from rabbit liver and a further purified MT-1 isoform were quantified by determination of sulfur, and the stoichiometric compositions of the metalloprotein complexes are characterized by determination of their sulfur-to-metal ratios. |
doi_str_mv | 10.1007/s00216-001-1164-z |
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Metallothionein (MT) isoforms are separated by CE and the elements Cu, Zn, Cd, and S are detected simultaneously by use of ICP-SFMS in the medium resolution mode. On-line isotope dilution is performed by continuous introduction of an isotopically enriched, species-unspecific spike solution after the separation step. 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subjects | Algorithms Cadmium - analysis Copper - analysis Electrophoresis, Capillary - instrumentation Electrophoresis, Capillary - methods Isotope Labeling - instrumentation Isotope Labeling - methods Mass Spectrometry - instrumentation Mass Spectrometry - methods Metallothionein - analysis Protein Isoforms - analysis Sulfur - analysis Time Factors Zinc - analysis |
title | Characterization and quantification of metallothionein isoforms by capillary electrophoresis-inductively coupled plasma-isotope-dilution mass spectrometry |
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