Bovine Liver Chromatin Fraction Contains Actin Polymerization Activity Inducing Micronuclei Formation when Injected into Prometaphase Cultured Cells
We previously reported that exogenous histone H1, when injected into mitotic cells, disrupts the synchronous progression of mitotic events by delaying chromosome decondensation. This strategy was utilized to determine whether any other interphase proteins are also able to disrupt normal mitotic proc...
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| Veröffentlicht in: | Cell Structure and Function 2002, Vol.27(1), pp.39-45 |
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| creator | Matsuoka, Yosuke Matsuoka, Yuriko Shibata, Satoshi Yoneda, Yoshihiro |
| description | We previously reported that exogenous histone H1, when injected into mitotic cells, disrupts the synchronous progression of mitotic events by delaying chromosome decondensation. This strategy was utilized to determine whether any other interphase proteins are also able to disrupt normal mitotic processes, when introduced into the mitotic phase. We found that a chromatin subfraction from bovine liver nuclei induced postmitotic micronuclei formation in a dose-dependent manner when injected into the prometaphase of rat kangaroo kidney epithelial (PtK2) cells. Close observation showed that, in the case of injected mitotic cells, the mitotic spindles were disrupted, chromosomes became scattered throughout the cytoplasm, and actin filaments were organized ectopically. In addition, when the fraction was injected into interphase cells, extra actin filaments were formed and microtubule organization was affected. In order to determine whether the micronuclei formation resulted from the ectopic formation of actin filaments, we examined the effect of the actin polymerization inhibitor, cytochalasin D. The results showed that the drug inhibited micronuclei formation. From these findings, we concluded that this chromatin subfraction contains actin polymerization activity, thus causing the disruption of mitotic spindles. |
| doi_str_mv | 10.1247/csf.27.39 |
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This strategy was utilized to determine whether any other interphase proteins are also able to disrupt normal mitotic processes, when introduced into the mitotic phase. We found that a chromatin subfraction from bovine liver nuclei induced postmitotic micronuclei formation in a dose-dependent manner when injected into the prometaphase of rat kangaroo kidney epithelial (PtK2) cells. Close observation showed that, in the case of injected mitotic cells, the mitotic spindles were disrupted, chromosomes became scattered throughout the cytoplasm, and actin filaments were organized ectopically. In addition, when the fraction was injected into interphase cells, extra actin filaments were formed and microtubule organization was affected. In order to determine whether the micronuclei formation resulted from the ectopic formation of actin filaments, we examined the effect of the actin polymerization inhibitor, cytochalasin D. The results showed that the drug inhibited micronuclei formation. From these findings, we concluded that this chromatin subfraction contains actin polymerization activity, thus causing the disruption of mitotic spindles.</description><identifier>ISSN: 0386-7196</identifier><identifier>EISSN: 1347-3700</identifier><identifier>DOI: 10.1247/csf.27.39</identifier><identifier>PMID: 11937717</identifier><language>eng</language><publisher>Japan: Japan Society for Cell Biology</publisher><subject>Actin Cytoskeleton - drug effects ; Actin Cytoskeleton - metabolism ; actin filament polymerization ; Animals ; Cattle ; cell cycle ; Chromatin - isolation & purification ; Chromatin - metabolism ; chromosome segregation ; Chromosomes - drug effects ; Chromosomes - physiology ; Cytochalasin D - pharmacology ; Epithelial Cells - cytology ; Epithelial Cells - drug effects ; Epithelial Cells - physiology ; Histones - pharmacology ; Interphase - physiology ; Liver - chemistry ; Macropodidae ; Metaphase - drug effects ; Metaphase - physiology ; Microinjections ; Micronuclei, Chromosome-Defective - drug effects ; Micronuclei, Chromosome-Defective - ultrastructure ; mitotic spindle ; Spindle Apparatus - drug effects ; Spindle Apparatus - ultrastructure</subject><ispartof>Cell Structure and Function, 2002, Vol.27(1), pp.39-45</ispartof><rights>2002 by Japan Society for Cell Biology</rights><rights>Copyright Japan Science and Technology Agency 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-4c68c6d6e3721cc8ae74cd067a647af9225f7c74d6fc7629aaa9307c616689983</citedby><cites>FETCH-LOGICAL-c423t-4c68c6d6e3721cc8ae74cd067a647af9225f7c74d6fc7629aaa9307c616689983</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11937717$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Matsuoka, Yosuke</creatorcontrib><creatorcontrib>Matsuoka, Yuriko</creatorcontrib><creatorcontrib>Shibata, Satoshi</creatorcontrib><creatorcontrib>Yoneda, Yoshihiro</creatorcontrib><title>Bovine Liver Chromatin Fraction Contains Actin Polymerization Activity Inducing Micronuclei Formation when Injected into Prometaphase Cultured Cells</title><title>Cell Structure and Function</title><addtitle>Cell Struct. Funct.</addtitle><description>We previously reported that exogenous histone H1, when injected into mitotic cells, disrupts the synchronous progression of mitotic events by delaying chromosome decondensation. This strategy was utilized to determine whether any other interphase proteins are also able to disrupt normal mitotic processes, when introduced into the mitotic phase. We found that a chromatin subfraction from bovine liver nuclei induced postmitotic micronuclei formation in a dose-dependent manner when injected into the prometaphase of rat kangaroo kidney epithelial (PtK2) cells. Close observation showed that, in the case of injected mitotic cells, the mitotic spindles were disrupted, chromosomes became scattered throughout the cytoplasm, and actin filaments were organized ectopically. In addition, when the fraction was injected into interphase cells, extra actin filaments were formed and microtubule organization was affected. In order to determine whether the micronuclei formation resulted from the ectopic formation of actin filaments, we examined the effect of the actin polymerization inhibitor, cytochalasin D. The results showed that the drug inhibited micronuclei formation. From these findings, we concluded that this chromatin subfraction contains actin polymerization activity, thus causing the disruption of mitotic spindles.</description><subject>Actin Cytoskeleton - drug effects</subject><subject>Actin Cytoskeleton - metabolism</subject><subject>actin filament polymerization</subject><subject>Animals</subject><subject>Cattle</subject><subject>cell cycle</subject><subject>Chromatin - isolation & purification</subject><subject>Chromatin - metabolism</subject><subject>chromosome segregation</subject><subject>Chromosomes - drug effects</subject><subject>Chromosomes - physiology</subject><subject>Cytochalasin D - pharmacology</subject><subject>Epithelial Cells - cytology</subject><subject>Epithelial Cells - drug effects</subject><subject>Epithelial Cells - physiology</subject><subject>Histones - pharmacology</subject><subject>Interphase - physiology</subject><subject>Liver - chemistry</subject><subject>Macropodidae</subject><subject>Metaphase - drug effects</subject><subject>Metaphase - physiology</subject><subject>Microinjections</subject><subject>Micronuclei, Chromosome-Defective - drug effects</subject><subject>Micronuclei, Chromosome-Defective - ultrastructure</subject><subject>mitotic spindle</subject><subject>Spindle Apparatus - drug effects</subject><subject>Spindle Apparatus - ultrastructure</subject><issn>0386-7196</issn><issn>1347-3700</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkV-LEzEUxYMobnf1wS8gAUHYh6n5M82dvAjrYHWh4j7oc4iZzDZlJqlJplI_hx_Y1Kku-JLAPT9Obs5B6AUlS8pqeGNSv2Sw5PIRWlBeQ8WBkMdoQXgjKqBSXKDLlHaEsBUR8BRdUCo5AIUF-vUuHJy3eOMONuJ2G8Oos_N4HbXJLnjcBp-18wnfmNP8LgzH0Ub3U_9RT8ODy0d867vJOH-PPzkTg5_MYB1ehzjO3I-t9YXZWZNth53PAd-Vp2zW-61OFrfTkKdYpNYOQ3qGnvR6SPb5-b5CX9fvv7Qfq83nD7ftzaYyNeO5qo1ojOiE5cCoMY22UJuu_FCLGnQvGVv1YKDuRG9AMKm1lpyAEVSIRsqGX6HXs-8-hu-TTVmNLpmygfY2TEkBXTWEAS_gq__AXZiiL7spWq_kSpSQSaGuZ6okkFK0vdpHN-p4VJSoU1GqFKUYKC4L-_LsOH0bbfdAnpspwNsZ2KWs7-0_QMfsSrh_reh8cPkgbHVU1vPfBDqneA</recordid><startdate>20020201</startdate><enddate>20020201</enddate><creator>Matsuoka, Yosuke</creator><creator>Matsuoka, Yuriko</creator><creator>Shibata, Satoshi</creator><creator>Yoneda, Yoshihiro</creator><general>Japan Society for Cell Biology</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20020201</creationdate><title>Bovine Liver Chromatin Fraction Contains Actin Polymerization Activity Inducing Micronuclei Formation when Injected into Prometaphase Cultured Cells</title><author>Matsuoka, Yosuke ; Matsuoka, Yuriko ; Shibata, Satoshi ; Yoneda, Yoshihiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-4c68c6d6e3721cc8ae74cd067a647af9225f7c74d6fc7629aaa9307c616689983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Actin Cytoskeleton - drug effects</topic><topic>Actin Cytoskeleton - metabolism</topic><topic>actin filament polymerization</topic><topic>Animals</topic><topic>Cattle</topic><topic>cell cycle</topic><topic>Chromatin - isolation & purification</topic><topic>Chromatin - metabolism</topic><topic>chromosome segregation</topic><topic>Chromosomes - drug effects</topic><topic>Chromosomes - physiology</topic><topic>Cytochalasin D - pharmacology</topic><topic>Epithelial Cells - cytology</topic><topic>Epithelial Cells - drug effects</topic><topic>Epithelial Cells - physiology</topic><topic>Histones - pharmacology</topic><topic>Interphase - physiology</topic><topic>Liver - chemistry</topic><topic>Macropodidae</topic><topic>Metaphase - drug effects</topic><topic>Metaphase - physiology</topic><topic>Microinjections</topic><topic>Micronuclei, Chromosome-Defective - drug effects</topic><topic>Micronuclei, Chromosome-Defective - ultrastructure</topic><topic>mitotic spindle</topic><topic>Spindle Apparatus - drug effects</topic><topic>Spindle Apparatus - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Matsuoka, Yosuke</creatorcontrib><creatorcontrib>Matsuoka, Yuriko</creatorcontrib><creatorcontrib>Shibata, Satoshi</creatorcontrib><creatorcontrib>Yoneda, Yoshihiro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell Structure and Function</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Matsuoka, Yosuke</au><au>Matsuoka, Yuriko</au><au>Shibata, Satoshi</au><au>Yoneda, Yoshihiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bovine Liver Chromatin Fraction Contains Actin Polymerization Activity Inducing Micronuclei Formation when Injected into Prometaphase Cultured Cells</atitle><jtitle>Cell Structure and Function</jtitle><addtitle>Cell Struct. Funct.</addtitle><date>2002-02-01</date><risdate>2002</risdate><volume>27</volume><issue>1</issue><spage>39</spage><epage>45</epage><pages>39-45</pages><issn>0386-7196</issn><eissn>1347-3700</eissn><abstract>We previously reported that exogenous histone H1, when injected into mitotic cells, disrupts the synchronous progression of mitotic events by delaying chromosome decondensation. This strategy was utilized to determine whether any other interphase proteins are also able to disrupt normal mitotic processes, when introduced into the mitotic phase. We found that a chromatin subfraction from bovine liver nuclei induced postmitotic micronuclei formation in a dose-dependent manner when injected into the prometaphase of rat kangaroo kidney epithelial (PtK2) cells. Close observation showed that, in the case of injected mitotic cells, the mitotic spindles were disrupted, chromosomes became scattered throughout the cytoplasm, and actin filaments were organized ectopically. In addition, when the fraction was injected into interphase cells, extra actin filaments were formed and microtubule organization was affected. In order to determine whether the micronuclei formation resulted from the ectopic formation of actin filaments, we examined the effect of the actin polymerization inhibitor, cytochalasin D. The results showed that the drug inhibited micronuclei formation. From these findings, we concluded that this chromatin subfraction contains actin polymerization activity, thus causing the disruption of mitotic spindles.</abstract><cop>Japan</cop><pub>Japan Society for Cell Biology</pub><pmid>11937717</pmid><doi>10.1247/csf.27.39</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Actin Cytoskeleton - drug effects Actin Cytoskeleton - metabolism actin filament polymerization Animals Cattle cell cycle Chromatin - isolation & purification Chromatin - metabolism chromosome segregation Chromosomes - drug effects Chromosomes - physiology Cytochalasin D - pharmacology Epithelial Cells - cytology Epithelial Cells - drug effects Epithelial Cells - physiology Histones - pharmacology Interphase - physiology Liver - chemistry Macropodidae Metaphase - drug effects Metaphase - physiology Microinjections Micronuclei, Chromosome-Defective - drug effects Micronuclei, Chromosome-Defective - ultrastructure mitotic spindle Spindle Apparatus - drug effects Spindle Apparatus - ultrastructure |
| title | Bovine Liver Chromatin Fraction Contains Actin Polymerization Activity Inducing Micronuclei Formation when Injected into Prometaphase Cultured Cells |
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