Aquaporin 1 Regulates GTP-Induced Rapid Gating of Water in Secretory Vesicles
The swelling of secretory vesicles has been implicated in exocytosis, but the underlying mechanism of vesicle swelling remains largely unknown. Zymogen granules (ZGs), the membrane-bound secretory vesicles in exocrine pancreas, swell in response to GTP mediated by a Gα i3protein. Evidence is present...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2002-04, Vol.99 (7), p.4720-4724 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Cho, Sang-Joon A. K. M. Abdus Sattar Jeong, Eun-Hwan Satchi, Mylan Cho, Jin Ah Dash, Sudhansu Mayes, Mary Sue Stromer, Marvin H. Jena, Bhanu P. |
| description | The swelling of secretory vesicles has been implicated in exocytosis, but the underlying mechanism of vesicle swelling remains largely unknown. Zymogen granules (ZGs), the membrane-bound secretory vesicles in exocrine pancreas, swell in response to GTP mediated by a Gα i3protein. Evidence is presented here that the water channel aquaporin-1 (AQP1) is present in the ZG membrane and participates in rapid GTP-induced vesicular water gating and swelling. Isolated ZGs exhibit low basal water permeability. However, exposure of granules to GTP results in a marked potentiation of water entry. Treatment of ZGs with the known water channel inhibitor Hg2+is accompanied by a reversible loss in both the basal and GTP-stimulatable water entry and vesicle swelling. Introduction of AQP1-specific antibody raised against the carboxyl-terminal domain of AQP1 blocks GTP-stimulable swelling of vesicles. Our results demonstrate that AQP1 associated at the ZG membrane is involved in basal as well as GTP-induced rapid gating of water in ZGs of the exocrine pancreas. |
| doi_str_mv | 10.1073/pnas.072083499 |
| format | Article |
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K. M. Abdus Sattar ; Jeong, Eun-Hwan ; Satchi, Mylan ; Cho, Jin Ah ; Dash, Sudhansu ; Mayes, Mary Sue ; Stromer, Marvin H. ; Jena, Bhanu P.</creator><creatorcontrib>Cho, Sang-Joon ; A. K. M. Abdus Sattar ; Jeong, Eun-Hwan ; Satchi, Mylan ; Cho, Jin Ah ; Dash, Sudhansu ; Mayes, Mary Sue ; Stromer, Marvin H. ; Jena, Bhanu P.</creatorcontrib><description>The swelling of secretory vesicles has been implicated in exocytosis, but the underlying mechanism of vesicle swelling remains largely unknown. Zymogen granules (ZGs), the membrane-bound secretory vesicles in exocrine pancreas, swell in response to GTP mediated by a Gα i3protein. Evidence is presented here that the water channel aquaporin-1 (AQP1) is present in the ZG membrane and participates in rapid GTP-induced vesicular water gating and swelling. Isolated ZGs exhibit low basal water permeability. However, exposure of granules to GTP results in a marked potentiation of water entry. Treatment of ZGs with the known water channel inhibitor Hg2+is accompanied by a reversible loss in both the basal and GTP-stimulatable water entry and vesicle swelling. Introduction of AQP1-specific antibody raised against the carboxyl-terminal domain of AQP1 blocks GTP-stimulable swelling of vesicles. Our results demonstrate that AQP1 associated at the ZG membrane is involved in basal as well as GTP-induced rapid gating of water in ZGs of the exocrine pancreas.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.072083499</identifier><identifier>PMID: 11917120</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Acinar cells ; Animals ; Antibodies ; Aquaporin 1 ; Aquaporins - physiology ; Biological Sciences ; Body Water - metabolism ; Cell membranes ; Cells ; Cellular biology ; Exocytosis ; Guanosine Triphosphate - pharmacology ; Membranes ; Mercuric Chloride - pharmacology ; Microscopy ; P branes ; Pancreas ; Physiological regulation ; Proteins ; Rats ; Secretion ; Secretory vesicles ; Secretory Vesicles - drug effects ; Secretory Vesicles - metabolism ; Swelling ; Water channels</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2002-04, Vol.99 (7), p.4720-4724</ispartof><rights>Copyright 1993-2002 National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Apr 2, 2002</rights><rights>Copyright © 2002, The National Academy of Sciences 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c552t-af0078bcb4ca1183e5babc9ff0625f5cf014b05e9bc4acce5cd50fc49d5aadff3</citedby><cites>FETCH-LOGICAL-c552t-af0078bcb4ca1183e5babc9ff0625f5cf014b05e9bc4acce5cd50fc49d5aadff3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/99/7.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3058370$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3058370$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11917120$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cho, Sang-Joon</creatorcontrib><creatorcontrib>A. K. M. Abdus Sattar</creatorcontrib><creatorcontrib>Jeong, Eun-Hwan</creatorcontrib><creatorcontrib>Satchi, Mylan</creatorcontrib><creatorcontrib>Cho, Jin Ah</creatorcontrib><creatorcontrib>Dash, Sudhansu</creatorcontrib><creatorcontrib>Mayes, Mary Sue</creatorcontrib><creatorcontrib>Stromer, Marvin H.</creatorcontrib><creatorcontrib>Jena, Bhanu P.</creatorcontrib><title>Aquaporin 1 Regulates GTP-Induced Rapid Gating of Water in Secretory Vesicles</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The swelling of secretory vesicles has been implicated in exocytosis, but the underlying mechanism of vesicle swelling remains largely unknown. Zymogen granules (ZGs), the membrane-bound secretory vesicles in exocrine pancreas, swell in response to GTP mediated by a Gα i3protein. 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Our results demonstrate that AQP1 associated at the ZG membrane is involved in basal as well as GTP-induced rapid gating of water in ZGs of the exocrine pancreas.</description><subject>Acinar cells</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Aquaporin 1</subject><subject>Aquaporins - physiology</subject><subject>Biological Sciences</subject><subject>Body Water - metabolism</subject><subject>Cell membranes</subject><subject>Cells</subject><subject>Cellular biology</subject><subject>Exocytosis</subject><subject>Guanosine Triphosphate - pharmacology</subject><subject>Membranes</subject><subject>Mercuric Chloride - pharmacology</subject><subject>Microscopy</subject><subject>P branes</subject><subject>Pancreas</subject><subject>Physiological regulation</subject><subject>Proteins</subject><subject>Rats</subject><subject>Secretion</subject><subject>Secretory vesicles</subject><subject>Secretory Vesicles - drug effects</subject><subject>Secretory Vesicles - metabolism</subject><subject>Swelling</subject><subject>Water channels</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kb9vEzEYhi0EoqGwMgGyGLpd-PwrtgeGqoK0UhGoFBgtn88OF13OV_sO0f8eRwlpy8Dk4X2eT6_1IvSSwJyAZO-G3uY5SAqKca0foRkBTaoF1_AYzQCorBSn_Ag9y3kNAFooeIqOCNFEEgoz9On0ZrJDTG2PCb7yq6mzo894ef2luuibyfkGX9mhbfDSjm2_wjHgH4VIuAhfvUt-jOkWf_e5dZ3Pz9GTYLvsX-zfY_Tt44frs_Pq8vPy4uz0snJC0LGyAUCq2tXcWUIU86K2tdMhwIKKIFwAwmsQXteOW-e8cI2A4LhuhLVNCOwYvd_dHaZ64xvn-zHZzgyp3dh0a6JtzcOkb3-aVfxlCGWS8OKf7P0UbyafR7Nps_NdZ3sfp2wkEVIpvijg23_AdZxSX_5mKBCmFaOqQPMd5FLMOflwKELAbFcy25XMYaUivLlf_w7fz3Kv31b8G2ttpOHliglT143-91jA1_8DS_5ql69zGeoAMBCKSWB_AHdWsBw</recordid><startdate>20020402</startdate><enddate>20020402</enddate><creator>Cho, Sang-Joon</creator><creator>A. 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| subjects | Acinar cells Animals Antibodies Aquaporin 1 Aquaporins - physiology Biological Sciences Body Water - metabolism Cell membranes Cells Cellular biology Exocytosis Guanosine Triphosphate - pharmacology Membranes Mercuric Chloride - pharmacology Microscopy P branes Pancreas Physiological regulation Proteins Rats Secretion Secretory vesicles Secretory Vesicles - drug effects Secretory Vesicles - metabolism Swelling Water channels |
| title | Aquaporin 1 Regulates GTP-Induced Rapid Gating of Water in Secretory Vesicles |
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