A comparison of immunocytochemical markers to identify bipolar cell types in human and monkey retina
As more human retinas affected with genetic or immune-based diseases become available for morphological analysis, it is important to identify immunocytochemical markers for specific subtypes of retinal neurons. In this study, we have focused on bipolar cell markers in central retina. We have done si...
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creator | HAVERKAMP, SILKE HAESELEER, FRANCOISE HENDRICKSON, ANITA |
description | As more human retinas affected with genetic or immune-based diseases
become available for morphological analysis, it is important to
identify immunocytochemical markers for specific subtypes of retinal
neurons. In this study, we have focused on bipolar cell markers in
central retina. We have done single and double labeling using several
antisera previously utilized in macaque monkey or human retinal studies
and two new antisera (1) to correlate combinations of antisera labeling
with morphological types of bipolar cells in human retina, and (2) to
compare human labeling patterns with those in monkey retina. Human
bipolar cells showed a wide range of labeling patterns with at least
ten different bipolar cell types identified from their anatomy and
marker content. Many bipolar cell bodies in the outer part of the inner
nuclear layer contained combinations of protein kinase C alpha
(PKCα), Islet-1, glycine, and Goα. Bipolar cells labeled with
these markers had axons terminating in the inner half of the inner
plexiform layer (IPL), consistent with ON bipolar cells. Bipolar cell
bodies adjacent to the amacrine cells and with axons in the outer half
of the IPL contained combinations of recoverin, glutamate
transporter-1, and PKCβ, or CD15 and calbindin. Bipolar cells
labeled with these markers were presumed OFF bipolar cells.
Calcium-binding protein 5 (CaB5) labeled both putative ON and OFF
bipolar cells. Using this cell labeling as a criteria, most cell bodies
close to the horizontal cells were ON bipolar cells and almost all
bipolar cells adjacent to the amacrine cells were OFF with a band in
the middle 2–3 cell bodies thick containing intermixed ON and OFF
bipolar cells. Differences were found between human and monkey bipolar
cell types labeled by calbindin, CaB5, and CD15. Two new types were
identified. One was morphologically similar to the DB3, but labeled for
CD15 and CaB5. The other had a calbindin-labeled cell body adjacent to
the horizontal cell bodies, but did not contain any accepted ON
markers. These results support the use of macaque monkey retina as a
model for human, but caution against the assumption that all labeling
patterns are identical in the two primates. |
doi_str_mv | 10.1017/S0952523803206015 |
format | Article |
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become available for morphological analysis, it is important to
identify immunocytochemical markers for specific subtypes of retinal
neurons. In this study, we have focused on bipolar cell markers in
central retina. We have done single and double labeling using several
antisera previously utilized in macaque monkey or human retinal studies
and two new antisera (1) to correlate combinations of antisera labeling
with morphological types of bipolar cells in human retina, and (2) to
compare human labeling patterns with those in monkey retina. Human
bipolar cells showed a wide range of labeling patterns with at least
ten different bipolar cell types identified from their anatomy and
marker content. Many bipolar cell bodies in the outer part of the inner
nuclear layer contained combinations of protein kinase C alpha
(PKCα), Islet-1, glycine, and Goα. Bipolar cells labeled with
these markers had axons terminating in the inner half of the inner
plexiform layer (IPL), consistent with ON bipolar cells. Bipolar cell
bodies adjacent to the amacrine cells and with axons in the outer half
of the IPL contained combinations of recoverin, glutamate
transporter-1, and PKCβ, or CD15 and calbindin. Bipolar cells
labeled with these markers were presumed OFF bipolar cells.
Calcium-binding protein 5 (CaB5) labeled both putative ON and OFF
bipolar cells. Using this cell labeling as a criteria, most cell bodies
close to the horizontal cells were ON bipolar cells and almost all
bipolar cells adjacent to the amacrine cells were OFF with a band in
the middle 2–3 cell bodies thick containing intermixed ON and OFF
bipolar cells. Differences were found between human and monkey bipolar
cell types labeled by calbindin, CaB5, and CD15. Two new types were
identified. One was morphologically similar to the DB3, but labeled for
CD15 and CaB5. The other had a calbindin-labeled cell body adjacent to
the horizontal cell bodies, but did not contain any accepted ON
markers. These results support the use of macaque monkey retina as a
model for human, but caution against the assumption that all labeling
patterns are identical in the two primates.</description><identifier>ISSN: 0952-5238</identifier><identifier>EISSN: 1469-8714</identifier><identifier>DOI: 10.1017/S0952523803206015</identifier><identifier>PMID: 15088712</identifier><language>eng</language><publisher>New York, USA: Cambridge University Press</publisher><subject>Aged ; Aged, 80 and over ; Animals ; Biological and medical sciences ; Biomarkers - analysis ; calbindin ; Calcium-binding protein 5 ; CD15 ; Cells ; Child ; Eye and associated structures. Visual pathways and centers. Vision ; Eye Proteins - analysis ; Female ; Fluorescent Antibody Technique, Indirect ; Fundamental and applied biological sciences. Psychology ; horizontal cells ; Humans ; Interneurons - chemistry ; Interneurons - cytology ; Islet-1 ; Kinases ; Labeling ; Macaca ; Macaca fascicularis ; PKCα ; PKCβ ; Primates ; Retina ; Retina - chemistry ; Retina - cytology ; Vertebrates: nervous system and sense organs</subject><ispartof>Visual neuroscience, 2003-11, Vol.20 (6), p.589-600</ispartof><rights>2003 Cambridge University Press</rights><rights>2005 INIST-CNRS</rights><rights>Copyright Cambridge University Press Nov 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c581t-9591a40f94b722c206742c86e960792790f83ced27b57499c21690d0e801aef33</citedby><cites>FETCH-LOGICAL-c581t-9591a40f94b722c206742c86e960792790f83ced27b57499c21690d0e801aef33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0952523803206015/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,314,780,784,27924,27925,55628</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15804666$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15088712$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HAVERKAMP, SILKE</creatorcontrib><creatorcontrib>HAESELEER, FRANCOISE</creatorcontrib><creatorcontrib>HENDRICKSON, ANITA</creatorcontrib><title>A comparison of immunocytochemical markers to identify bipolar cell types in human and monkey retina</title><title>Visual neuroscience</title><addtitle>Vis Neurosci</addtitle><description>As more human retinas affected with genetic or immune-based diseases
become available for morphological analysis, it is important to
identify immunocytochemical markers for specific subtypes of retinal
neurons. In this study, we have focused on bipolar cell markers in
central retina. We have done single and double labeling using several
antisera previously utilized in macaque monkey or human retinal studies
and two new antisera (1) to correlate combinations of antisera labeling
with morphological types of bipolar cells in human retina, and (2) to
compare human labeling patterns with those in monkey retina. Human
bipolar cells showed a wide range of labeling patterns with at least
ten different bipolar cell types identified from their anatomy and
marker content. Many bipolar cell bodies in the outer part of the inner
nuclear layer contained combinations of protein kinase C alpha
(PKCα), Islet-1, glycine, and Goα. Bipolar cells labeled with
these markers had axons terminating in the inner half of the inner
plexiform layer (IPL), consistent with ON bipolar cells. Bipolar cell
bodies adjacent to the amacrine cells and with axons in the outer half
of the IPL contained combinations of recoverin, glutamate
transporter-1, and PKCβ, or CD15 and calbindin. Bipolar cells
labeled with these markers were presumed OFF bipolar cells.
Calcium-binding protein 5 (CaB5) labeled both putative ON and OFF
bipolar cells. Using this cell labeling as a criteria, most cell bodies
close to the horizontal cells were ON bipolar cells and almost all
bipolar cells adjacent to the amacrine cells were OFF with a band in
the middle 2–3 cell bodies thick containing intermixed ON and OFF
bipolar cells. Differences were found between human and monkey bipolar
cell types labeled by calbindin, CaB5, and CD15. Two new types were
identified. One was morphologically similar to the DB3, but labeled for
CD15 and CaB5. The other had a calbindin-labeled cell body adjacent to
the horizontal cell bodies, but did not contain any accepted ON
markers. These results support the use of macaque monkey retina as a
model for human, but caution against the assumption that all labeling
patterns are identical in the two primates.</description><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - analysis</subject><subject>calbindin</subject><subject>Calcium-binding protein 5</subject><subject>CD15</subject><subject>Cells</subject><subject>Child</subject><subject>Eye and associated structures. Visual pathways and centers. Vision</subject><subject>Eye Proteins - analysis</subject><subject>Female</subject><subject>Fluorescent Antibody Technique, Indirect</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>horizontal cells</subject><subject>Humans</subject><subject>Interneurons - chemistry</subject><subject>Interneurons - cytology</subject><subject>Islet-1</subject><subject>Kinases</subject><subject>Labeling</subject><subject>Macaca</subject><subject>Macaca fascicularis</subject><subject>PKCα</subject><subject>PKCβ</subject><subject>Primates</subject><subject>Retina</subject><subject>Retina - chemistry</subject><subject>Retina - cytology</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0952-5238</issn><issn>1469-8714</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkUuLFDEUhYMoTjv6A9xIEHRXmvdjOTTaKgMiPnAXUqmUk5mqpE2qwPr3puhCB0VcZXG-e3POPQA8xugFRli-_Ig0J5xQhShBAmF-B-wwE7pRErO7YLfKzaqfgQelXCOEKeb0PjjDHKnKkB3oLqBL49HmUFKEqYdhHOeY3DIld-XH4OwAR5tvfC5wSjB0Pk6hX2AbjmmwGTo_DHBajr7AEOHVPNoIbezgmOKNX2D2U4j2IbjX26H4R9t7Dj6_fvVp_6a5fH94u7-4bBxXeGo019gy1GvWSkJcjSQZcUp4LZDURGrUK-p8R2TLJdPaESw06pBXCFvfU3oOnp_2HnP6PvsymTGU1aGNPs3FSMwlp5z9F8SaMo6RquDTP8DrNOdYQ1RG1Wtiun6LT5DLqZTse3PMoR5tMRiZtSjzV1F15sm2eG5H3_2e2JqpwLMNsKWW0GcbXSi3OIWYEKJyzYkLZfI_fum1MiMkldyIwwfzbn9g8uvhi5GVp5tZO7Y5dN_8rUj_tPsTpnW3cg</recordid><startdate>20031101</startdate><enddate>20031101</enddate><creator>HAVERKAMP, SILKE</creator><creator>HAESELEER, FRANCOISE</creator><creator>HENDRICKSON, ANITA</creator><general>Cambridge University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88G</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20031101</creationdate><title>A comparison of immunocytochemical markers to identify bipolar cell types in human and monkey retina</title><author>HAVERKAMP, SILKE ; HAESELEER, FRANCOISE ; HENDRICKSON, ANITA</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c581t-9591a40f94b722c206742c86e960792790f83ced27b57499c21690d0e801aef33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biomarkers - analysis</topic><topic>calbindin</topic><topic>Calcium-binding protein 5</topic><topic>CD15</topic><topic>Cells</topic><topic>Child</topic><topic>Eye and associated structures. Visual pathways and centers. Vision</topic><topic>Eye Proteins - analysis</topic><topic>Female</topic><topic>Fluorescent Antibody Technique, Indirect</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>horizontal cells</topic><topic>Humans</topic><topic>Interneurons - chemistry</topic><topic>Interneurons - cytology</topic><topic>Islet-1</topic><topic>Kinases</topic><topic>Labeling</topic><topic>Macaca</topic><topic>Macaca fascicularis</topic><topic>PKCα</topic><topic>PKCβ</topic><topic>Primates</topic><topic>Retina</topic><topic>Retina - chemistry</topic><topic>Retina - cytology</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HAVERKAMP, SILKE</creatorcontrib><creatorcontrib>HAESELEER, FRANCOISE</creatorcontrib><creatorcontrib>HENDRICKSON, ANITA</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Psychology Database (ProQuest)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Visual neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HAVERKAMP, SILKE</au><au>HAESELEER, FRANCOISE</au><au>HENDRICKSON, ANITA</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A comparison of immunocytochemical markers to identify bipolar cell types in human and monkey retina</atitle><jtitle>Visual neuroscience</jtitle><addtitle>Vis Neurosci</addtitle><date>2003-11-01</date><risdate>2003</risdate><volume>20</volume><issue>6</issue><spage>589</spage><epage>600</epage><pages>589-600</pages><issn>0952-5238</issn><eissn>1469-8714</eissn><abstract>As more human retinas affected with genetic or immune-based diseases
become available for morphological analysis, it is important to
identify immunocytochemical markers for specific subtypes of retinal
neurons. In this study, we have focused on bipolar cell markers in
central retina. We have done single and double labeling using several
antisera previously utilized in macaque monkey or human retinal studies
and two new antisera (1) to correlate combinations of antisera labeling
with morphological types of bipolar cells in human retina, and (2) to
compare human labeling patterns with those in monkey retina. Human
bipolar cells showed a wide range of labeling patterns with at least
ten different bipolar cell types identified from their anatomy and
marker content. Many bipolar cell bodies in the outer part of the inner
nuclear layer contained combinations of protein kinase C alpha
(PKCα), Islet-1, glycine, and Goα. Bipolar cells labeled with
these markers had axons terminating in the inner half of the inner
plexiform layer (IPL), consistent with ON bipolar cells. Bipolar cell
bodies adjacent to the amacrine cells and with axons in the outer half
of the IPL contained combinations of recoverin, glutamate
transporter-1, and PKCβ, or CD15 and calbindin. Bipolar cells
labeled with these markers were presumed OFF bipolar cells.
Calcium-binding protein 5 (CaB5) labeled both putative ON and OFF
bipolar cells. Using this cell labeling as a criteria, most cell bodies
close to the horizontal cells were ON bipolar cells and almost all
bipolar cells adjacent to the amacrine cells were OFF with a band in
the middle 2–3 cell bodies thick containing intermixed ON and OFF
bipolar cells. Differences were found between human and monkey bipolar
cell types labeled by calbindin, CaB5, and CD15. Two new types were
identified. One was morphologically similar to the DB3, but labeled for
CD15 and CaB5. The other had a calbindin-labeled cell body adjacent to
the horizontal cell bodies, but did not contain any accepted ON
markers. These results support the use of macaque monkey retina as a
model for human, but caution against the assumption that all labeling
patterns are identical in the two primates.</abstract><cop>New York, USA</cop><pub>Cambridge University Press</pub><pmid>15088712</pmid><doi>10.1017/S0952523803206015</doi><tpages>12</tpages></addata></record> |
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subjects | Aged Aged, 80 and over Animals Biological and medical sciences Biomarkers - analysis calbindin Calcium-binding protein 5 CD15 Cells Child Eye and associated structures. Visual pathways and centers. Vision Eye Proteins - analysis Female Fluorescent Antibody Technique, Indirect Fundamental and applied biological sciences. Psychology horizontal cells Humans Interneurons - chemistry Interneurons - cytology Islet-1 Kinases Labeling Macaca Macaca fascicularis PKCα PKCβ Primates Retina Retina - chemistry Retina - cytology Vertebrates: nervous system and sense organs |
title | A comparison of immunocytochemical markers to identify bipolar cell types in human and monkey retina |
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