A comparison of immunocytochemical markers to identify bipolar cell types in human and monkey retina

As more human retinas affected with genetic or immune-based diseases become available for morphological analysis, it is important to identify immunocytochemical markers for specific subtypes of retinal neurons. In this study, we have focused on bipolar cell markers in central retina. We have done si...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Visual neuroscience 2003-11, Vol.20 (6), p.589-600
Hauptverfasser: HAVERKAMP, SILKE, HAESELEER, FRANCOISE, HENDRICKSON, ANITA
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 600
container_issue 6
container_start_page 589
container_title Visual neuroscience
container_volume 20
creator HAVERKAMP, SILKE
HAESELEER, FRANCOISE
HENDRICKSON, ANITA
description As more human retinas affected with genetic or immune-based diseases become available for morphological analysis, it is important to identify immunocytochemical markers for specific subtypes of retinal neurons. In this study, we have focused on bipolar cell markers in central retina. We have done single and double labeling using several antisera previously utilized in macaque monkey or human retinal studies and two new antisera (1) to correlate combinations of antisera labeling with morphological types of bipolar cells in human retina, and (2) to compare human labeling patterns with those in monkey retina. Human bipolar cells showed a wide range of labeling patterns with at least ten different bipolar cell types identified from their anatomy and marker content. Many bipolar cell bodies in the outer part of the inner nuclear layer contained combinations of protein kinase C alpha (PKCα), Islet-1, glycine, and Goα. Bipolar cells labeled with these markers had axons terminating in the inner half of the inner plexiform layer (IPL), consistent with ON bipolar cells. Bipolar cell bodies adjacent to the amacrine cells and with axons in the outer half of the IPL contained combinations of recoverin, glutamate transporter-1, and PKCβ, or CD15 and calbindin. Bipolar cells labeled with these markers were presumed OFF bipolar cells. Calcium-binding protein 5 (CaB5) labeled both putative ON and OFF bipolar cells. Using this cell labeling as a criteria, most cell bodies close to the horizontal cells were ON bipolar cells and almost all bipolar cells adjacent to the amacrine cells were OFF with a band in the middle 2–3 cell bodies thick containing intermixed ON and OFF bipolar cells. Differences were found between human and monkey bipolar cell types labeled by calbindin, CaB5, and CD15. Two new types were identified. One was morphologically similar to the DB3, but labeled for CD15 and CaB5. The other had a calbindin-labeled cell body adjacent to the horizontal cell bodies, but did not contain any accepted ON markers. These results support the use of macaque monkey retina as a model for human, but caution against the assumption that all labeling patterns are identical in the two primates.
doi_str_mv 10.1017/S0952523803206015
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71575354</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cupid>10_1017_S0952523803206015</cupid><sourcerecordid>1457083411</sourcerecordid><originalsourceid>FETCH-LOGICAL-c581t-9591a40f94b722c206742c86e960792790f83ced27b57499c21690d0e801aef33</originalsourceid><addsrcrecordid>eNqFkUuLFDEUhYMoTjv6A9xIEHRXmvdjOTTaKgMiPnAXUqmUk5mqpE2qwPr3puhCB0VcZXG-e3POPQA8xugFRli-_Ig0J5xQhShBAmF-B-wwE7pRErO7YLfKzaqfgQelXCOEKeb0PjjDHKnKkB3oLqBL49HmUFKEqYdhHOeY3DIld-XH4OwAR5tvfC5wSjB0Pk6hX2AbjmmwGTo_DHBajr7AEOHVPNoIbezgmOKNX2D2U4j2IbjX26H4R9t7Dj6_fvVp_6a5fH94u7-4bBxXeGo019gy1GvWSkJcjSQZcUp4LZDURGrUK-p8R2TLJdPaESw06pBXCFvfU3oOnp_2HnP6PvsymTGU1aGNPs3FSMwlp5z9F8SaMo6RquDTP8DrNOdYQ1RG1Wtiun6LT5DLqZTse3PMoR5tMRiZtSjzV1F15sm2eG5H3_2e2JqpwLMNsKWW0GcbXSi3OIWYEKJyzYkLZfI_fum1MiMkldyIwwfzbn9g8uvhi5GVp5tZO7Y5dN_8rUj_tPsTpnW3cg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>198315133</pqid></control><display><type>article</type><title>A comparison of immunocytochemical markers to identify bipolar cell types in human and monkey retina</title><source>MEDLINE</source><source>Cambridge University Press Journals Complete</source><creator>HAVERKAMP, SILKE ; HAESELEER, FRANCOISE ; HENDRICKSON, ANITA</creator><creatorcontrib>HAVERKAMP, SILKE ; HAESELEER, FRANCOISE ; HENDRICKSON, ANITA</creatorcontrib><description>As more human retinas affected with genetic or immune-based diseases become available for morphological analysis, it is important to identify immunocytochemical markers for specific subtypes of retinal neurons. In this study, we have focused on bipolar cell markers in central retina. We have done single and double labeling using several antisera previously utilized in macaque monkey or human retinal studies and two new antisera (1) to correlate combinations of antisera labeling with morphological types of bipolar cells in human retina, and (2) to compare human labeling patterns with those in monkey retina. Human bipolar cells showed a wide range of labeling patterns with at least ten different bipolar cell types identified from their anatomy and marker content. Many bipolar cell bodies in the outer part of the inner nuclear layer contained combinations of protein kinase C alpha (PKCα), Islet-1, glycine, and Goα. Bipolar cells labeled with these markers had axons terminating in the inner half of the inner plexiform layer (IPL), consistent with ON bipolar cells. Bipolar cell bodies adjacent to the amacrine cells and with axons in the outer half of the IPL contained combinations of recoverin, glutamate transporter-1, and PKCβ, or CD15 and calbindin. Bipolar cells labeled with these markers were presumed OFF bipolar cells. Calcium-binding protein 5 (CaB5) labeled both putative ON and OFF bipolar cells. Using this cell labeling as a criteria, most cell bodies close to the horizontal cells were ON bipolar cells and almost all bipolar cells adjacent to the amacrine cells were OFF with a band in the middle 2–3 cell bodies thick containing intermixed ON and OFF bipolar cells. Differences were found between human and monkey bipolar cell types labeled by calbindin, CaB5, and CD15. Two new types were identified. One was morphologically similar to the DB3, but labeled for CD15 and CaB5. The other had a calbindin-labeled cell body adjacent to the horizontal cell bodies, but did not contain any accepted ON markers. These results support the use of macaque monkey retina as a model for human, but caution against the assumption that all labeling patterns are identical in the two primates.</description><identifier>ISSN: 0952-5238</identifier><identifier>EISSN: 1469-8714</identifier><identifier>DOI: 10.1017/S0952523803206015</identifier><identifier>PMID: 15088712</identifier><language>eng</language><publisher>New York, USA: Cambridge University Press</publisher><subject>Aged ; Aged, 80 and over ; Animals ; Biological and medical sciences ; Biomarkers - analysis ; calbindin ; Calcium-binding protein 5 ; CD15 ; Cells ; Child ; Eye and associated structures. Visual pathways and centers. Vision ; Eye Proteins - analysis ; Female ; Fluorescent Antibody Technique, Indirect ; Fundamental and applied biological sciences. Psychology ; horizontal cells ; Humans ; Interneurons - chemistry ; Interneurons - cytology ; Islet-1 ; Kinases ; Labeling ; Macaca ; Macaca fascicularis ; PKCα ; PKCβ ; Primates ; Retina ; Retina - chemistry ; Retina - cytology ; Vertebrates: nervous system and sense organs</subject><ispartof>Visual neuroscience, 2003-11, Vol.20 (6), p.589-600</ispartof><rights>2003 Cambridge University Press</rights><rights>2005 INIST-CNRS</rights><rights>Copyright Cambridge University Press Nov 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c581t-9591a40f94b722c206742c86e960792790f83ced27b57499c21690d0e801aef33</citedby><cites>FETCH-LOGICAL-c581t-9591a40f94b722c206742c86e960792790f83ced27b57499c21690d0e801aef33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0952523803206015/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,314,780,784,27924,27925,55628</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=15804666$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15088712$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HAVERKAMP, SILKE</creatorcontrib><creatorcontrib>HAESELEER, FRANCOISE</creatorcontrib><creatorcontrib>HENDRICKSON, ANITA</creatorcontrib><title>A comparison of immunocytochemical markers to identify bipolar cell types in human and monkey retina</title><title>Visual neuroscience</title><addtitle>Vis Neurosci</addtitle><description>As more human retinas affected with genetic or immune-based diseases become available for morphological analysis, it is important to identify immunocytochemical markers for specific subtypes of retinal neurons. In this study, we have focused on bipolar cell markers in central retina. We have done single and double labeling using several antisera previously utilized in macaque monkey or human retinal studies and two new antisera (1) to correlate combinations of antisera labeling with morphological types of bipolar cells in human retina, and (2) to compare human labeling patterns with those in monkey retina. Human bipolar cells showed a wide range of labeling patterns with at least ten different bipolar cell types identified from their anatomy and marker content. Many bipolar cell bodies in the outer part of the inner nuclear layer contained combinations of protein kinase C alpha (PKCα), Islet-1, glycine, and Goα. Bipolar cells labeled with these markers had axons terminating in the inner half of the inner plexiform layer (IPL), consistent with ON bipolar cells. Bipolar cell bodies adjacent to the amacrine cells and with axons in the outer half of the IPL contained combinations of recoverin, glutamate transporter-1, and PKCβ, or CD15 and calbindin. Bipolar cells labeled with these markers were presumed OFF bipolar cells. Calcium-binding protein 5 (CaB5) labeled both putative ON and OFF bipolar cells. Using this cell labeling as a criteria, most cell bodies close to the horizontal cells were ON bipolar cells and almost all bipolar cells adjacent to the amacrine cells were OFF with a band in the middle 2–3 cell bodies thick containing intermixed ON and OFF bipolar cells. Differences were found between human and monkey bipolar cell types labeled by calbindin, CaB5, and CD15. Two new types were identified. One was morphologically similar to the DB3, but labeled for CD15 and CaB5. The other had a calbindin-labeled cell body adjacent to the horizontal cell bodies, but did not contain any accepted ON markers. These results support the use of macaque monkey retina as a model for human, but caution against the assumption that all labeling patterns are identical in the two primates.</description><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - analysis</subject><subject>calbindin</subject><subject>Calcium-binding protein 5</subject><subject>CD15</subject><subject>Cells</subject><subject>Child</subject><subject>Eye and associated structures. Visual pathways and centers. Vision</subject><subject>Eye Proteins - analysis</subject><subject>Female</subject><subject>Fluorescent Antibody Technique, Indirect</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>horizontal cells</subject><subject>Humans</subject><subject>Interneurons - chemistry</subject><subject>Interneurons - cytology</subject><subject>Islet-1</subject><subject>Kinases</subject><subject>Labeling</subject><subject>Macaca</subject><subject>Macaca fascicularis</subject><subject>PKCα</subject><subject>PKCβ</subject><subject>Primates</subject><subject>Retina</subject><subject>Retina - chemistry</subject><subject>Retina - cytology</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0952-5238</issn><issn>1469-8714</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkUuLFDEUhYMoTjv6A9xIEHRXmvdjOTTaKgMiPnAXUqmUk5mqpE2qwPr3puhCB0VcZXG-e3POPQA8xugFRli-_Ig0J5xQhShBAmF-B-wwE7pRErO7YLfKzaqfgQelXCOEKeb0PjjDHKnKkB3oLqBL49HmUFKEqYdhHOeY3DIld-XH4OwAR5tvfC5wSjB0Pk6hX2AbjmmwGTo_DHBajr7AEOHVPNoIbezgmOKNX2D2U4j2IbjX26H4R9t7Dj6_fvVp_6a5fH94u7-4bBxXeGo019gy1GvWSkJcjSQZcUp4LZDURGrUK-p8R2TLJdPaESw06pBXCFvfU3oOnp_2HnP6PvsymTGU1aGNPs3FSMwlp5z9F8SaMo6RquDTP8DrNOdYQ1RG1Wtiun6LT5DLqZTse3PMoR5tMRiZtSjzV1F15sm2eG5H3_2e2JqpwLMNsKWW0GcbXSi3OIWYEKJyzYkLZfI_fum1MiMkldyIwwfzbn9g8uvhi5GVp5tZO7Y5dN_8rUj_tPsTpnW3cg</recordid><startdate>20031101</startdate><enddate>20031101</enddate><creator>HAVERKAMP, SILKE</creator><creator>HAESELEER, FRANCOISE</creator><creator>HENDRICKSON, ANITA</creator><general>Cambridge University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88G</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20031101</creationdate><title>A comparison of immunocytochemical markers to identify bipolar cell types in human and monkey retina</title><author>HAVERKAMP, SILKE ; HAESELEER, FRANCOISE ; HENDRICKSON, ANITA</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c581t-9591a40f94b722c206742c86e960792790f83ced27b57499c21690d0e801aef33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biomarkers - analysis</topic><topic>calbindin</topic><topic>Calcium-binding protein 5</topic><topic>CD15</topic><topic>Cells</topic><topic>Child</topic><topic>Eye and associated structures. Visual pathways and centers. Vision</topic><topic>Eye Proteins - analysis</topic><topic>Female</topic><topic>Fluorescent Antibody Technique, Indirect</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>horizontal cells</topic><topic>Humans</topic><topic>Interneurons - chemistry</topic><topic>Interneurons - cytology</topic><topic>Islet-1</topic><topic>Kinases</topic><topic>Labeling</topic><topic>Macaca</topic><topic>Macaca fascicularis</topic><topic>PKCα</topic><topic>PKCβ</topic><topic>Primates</topic><topic>Retina</topic><topic>Retina - chemistry</topic><topic>Retina - cytology</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HAVERKAMP, SILKE</creatorcontrib><creatorcontrib>HAESELEER, FRANCOISE</creatorcontrib><creatorcontrib>HENDRICKSON, ANITA</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Psychology Database (ProQuest)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Visual neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HAVERKAMP, SILKE</au><au>HAESELEER, FRANCOISE</au><au>HENDRICKSON, ANITA</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A comparison of immunocytochemical markers to identify bipolar cell types in human and monkey retina</atitle><jtitle>Visual neuroscience</jtitle><addtitle>Vis Neurosci</addtitle><date>2003-11-01</date><risdate>2003</risdate><volume>20</volume><issue>6</issue><spage>589</spage><epage>600</epage><pages>589-600</pages><issn>0952-5238</issn><eissn>1469-8714</eissn><abstract>As more human retinas affected with genetic or immune-based diseases become available for morphological analysis, it is important to identify immunocytochemical markers for specific subtypes of retinal neurons. In this study, we have focused on bipolar cell markers in central retina. We have done single and double labeling using several antisera previously utilized in macaque monkey or human retinal studies and two new antisera (1) to correlate combinations of antisera labeling with morphological types of bipolar cells in human retina, and (2) to compare human labeling patterns with those in monkey retina. Human bipolar cells showed a wide range of labeling patterns with at least ten different bipolar cell types identified from their anatomy and marker content. Many bipolar cell bodies in the outer part of the inner nuclear layer contained combinations of protein kinase C alpha (PKCα), Islet-1, glycine, and Goα. Bipolar cells labeled with these markers had axons terminating in the inner half of the inner plexiform layer (IPL), consistent with ON bipolar cells. Bipolar cell bodies adjacent to the amacrine cells and with axons in the outer half of the IPL contained combinations of recoverin, glutamate transporter-1, and PKCβ, or CD15 and calbindin. Bipolar cells labeled with these markers were presumed OFF bipolar cells. Calcium-binding protein 5 (CaB5) labeled both putative ON and OFF bipolar cells. Using this cell labeling as a criteria, most cell bodies close to the horizontal cells were ON bipolar cells and almost all bipolar cells adjacent to the amacrine cells were OFF with a band in the middle 2–3 cell bodies thick containing intermixed ON and OFF bipolar cells. Differences were found between human and monkey bipolar cell types labeled by calbindin, CaB5, and CD15. Two new types were identified. One was morphologically similar to the DB3, but labeled for CD15 and CaB5. The other had a calbindin-labeled cell body adjacent to the horizontal cell bodies, but did not contain any accepted ON markers. These results support the use of macaque monkey retina as a model for human, but caution against the assumption that all labeling patterns are identical in the two primates.</abstract><cop>New York, USA</cop><pub>Cambridge University Press</pub><pmid>15088712</pmid><doi>10.1017/S0952523803206015</doi><tpages>12</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0952-5238
ispartof Visual neuroscience, 2003-11, Vol.20 (6), p.589-600
issn 0952-5238
1469-8714
language eng
recordid cdi_proquest_miscellaneous_71575354
source MEDLINE; Cambridge University Press Journals Complete
subjects Aged
Aged, 80 and over
Animals
Biological and medical sciences
Biomarkers - analysis
calbindin
Calcium-binding protein 5
CD15
Cells
Child
Eye and associated structures. Visual pathways and centers. Vision
Eye Proteins - analysis
Female
Fluorescent Antibody Technique, Indirect
Fundamental and applied biological sciences. Psychology
horizontal cells
Humans
Interneurons - chemistry
Interneurons - cytology
Islet-1
Kinases
Labeling
Macaca
Macaca fascicularis
PKCα
PKCβ
Primates
Retina
Retina - chemistry
Retina - cytology
Vertebrates: nervous system and sense organs
title A comparison of immunocytochemical markers to identify bipolar cell types in human and monkey retina
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T23%3A00%3A10IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20comparison%20of%20immunocytochemical%20markers%20to%20identify%20bipolar%20cell%20types%20in%20human%20and%20monkey%20retina&rft.jtitle=Visual%20neuroscience&rft.au=HAVERKAMP,%20SILKE&rft.date=2003-11-01&rft.volume=20&rft.issue=6&rft.spage=589&rft.epage=600&rft.pages=589-600&rft.issn=0952-5238&rft.eissn=1469-8714&rft_id=info:doi/10.1017/S0952523803206015&rft_dat=%3Cproquest_cross%3E1457083411%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=198315133&rft_id=info:pmid/15088712&rft_cupid=10_1017_S0952523803206015&rfr_iscdi=true