Liquid Chromatography−Mass Spectrometry of cis- and all-trans-Lycopene in Human Serum and Prostate Tissue after Dietary Supplementation with Tomato Sauce
Several epidemiological studies suggest a lower incidence of prostate cancer in men who routinely consume tomato products. Tomatoes are the primary dietary source of lycopene, which is among the most potent antioxidants of the carotenoids. Men with clinical stage T1 or T2 prostate adenocarcinoma wer...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2002-04, Vol.50 (8), p.2214-2219 |
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| creator | van Breemen, Richard B Xu, Xiaoying Viana, Marlos A Chen, Longwen Stacewicz-Sapuntzakis, Maria Duncan, Claudine Bowen, Phyllis E Sharifi, Roohollah |
| description | Several epidemiological studies suggest a lower incidence of prostate cancer in men who routinely consume tomato products. Tomatoes are the primary dietary source of lycopene, which is among the most potent antioxidants of the carotenoids. Men with clinical stage T1 or T2 prostate adenocarcinoma were recruited (n = 32) and consumed tomato sauce based pasta dishes for 3 weeks (equivalent to 30 mg of lycopene per day) before radical prostectomy. Prostate tissue from needle biopsy just before intervention and prostectomy after supplementation from a subset of 11 subjects was evaluated for both total lycopene and lycopene geometrical isomer ratios. A gradient HPLC system using a C18 column with UV−vis absorbance detection was used to measure total lycopene. Because the absorbance detector was insufficiently sensitive, HPLC with a C30 column and positive ion atmospheric pressure chemical ionization mass spectrometric (LC-MS) detection was developed as a new assay to measure the ratio of lycopene cis/trans isomers in these samples. The limit of detection of the LC-MS method was determined to be 0.93 pmol of lycopene on-column, and a linear response was obtained over 3 orders of magnitude. Total lycopene in serum increased 2.0-fold from 35.6 to 69.9 μg/dL (from 0.664 to 1.30 μM) as a result of dietary supplementation with tomato sauce, whereas total lycopene in prostate tissue increased 3.0-fold from 0.196 to 0.582 ng/mg of tissue (from 0.365 to 1.09 pmol/mg). all-trans-Lycopene and at least 14 cis-isomer peaks were detected in prostate tissue and serum. The mean proportion of all-trans-lycopene in prostate tissue was ∼12.4% of total lycopene before supplementation but increased to 22.7% after dietary intervention with tomato sauce. In serum there was only a 2.8% but statistically significant increase in the proportion of all-trans-lycopene after intervention. These results indicate that short-term supplementation with tomato sauce containing primarily all-trans-lycopene (83% of total lycopene) results in substantial increases in total lycopene in serum and prostate and a substantial increase in all-trans-lycopene in prostate but relatively less in serum. Keywords: cis- and all-trans-lycopene; LC-MS; APCI; prostate cancer; tomato sauce; dietary supplement |
| doi_str_mv | 10.1021/jf0110351 |
| format | Article |
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Tomatoes are the primary dietary source of lycopene, which is among the most potent antioxidants of the carotenoids. Men with clinical stage T1 or T2 prostate adenocarcinoma were recruited (n = 32) and consumed tomato sauce based pasta dishes for 3 weeks (equivalent to 30 mg of lycopene per day) before radical prostectomy. Prostate tissue from needle biopsy just before intervention and prostectomy after supplementation from a subset of 11 subjects was evaluated for both total lycopene and lycopene geometrical isomer ratios. A gradient HPLC system using a C18 column with UV−vis absorbance detection was used to measure total lycopene. Because the absorbance detector was insufficiently sensitive, HPLC with a C30 column and positive ion atmospheric pressure chemical ionization mass spectrometric (LC-MS) detection was developed as a new assay to measure the ratio of lycopene cis/trans isomers in these samples. The limit of detection of the LC-MS method was determined to be 0.93 pmol of lycopene on-column, and a linear response was obtained over 3 orders of magnitude. Total lycopene in serum increased 2.0-fold from 35.6 to 69.9 μg/dL (from 0.664 to 1.30 μM) as a result of dietary supplementation with tomato sauce, whereas total lycopene in prostate tissue increased 3.0-fold from 0.196 to 0.582 ng/mg of tissue (from 0.365 to 1.09 pmol/mg). all-trans-Lycopene and at least 14 cis-isomer peaks were detected in prostate tissue and serum. The mean proportion of all-trans-lycopene in prostate tissue was ∼12.4% of total lycopene before supplementation but increased to 22.7% after dietary intervention with tomato sauce. In serum there was only a 2.8% but statistically significant increase in the proportion of all-trans-lycopene after intervention. These results indicate that short-term supplementation with tomato sauce containing primarily all-trans-lycopene (83% of total lycopene) results in substantial increases in total lycopene in serum and prostate and a substantial increase in all-trans-lycopene in prostate but relatively less in serum. Keywords: cis- and all-trans-lycopene; LC-MS; APCI; prostate cancer; tomato sauce; dietary supplement</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf0110351</identifier><identifier>PMID: 11929273</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Adenocarcinoma - metabolism ; Adenocarcinoma - surgery ; Aged ; Biological and medical sciences ; Biopsy, Needle ; Carcinogenesis, carcinogens and anticarcinogens ; Carotenoids - administration & dosage ; Carotenoids - analysis ; Carotenoids - blood ; Chromatography, High Pressure Liquid ; Chromatography, Liquid ; Diet ; Foods and miscellaneous ; Humans ; Isomerism ; Lycopene ; Lycopersicon esculentum ; Male ; Mass Spectrometry ; Medical sciences ; Middle Aged ; Prostate - chemistry ; Prostatectomy ; Prostatic Neoplasms - metabolism ; Prostatic Neoplasms - surgery ; Tumors</subject><ispartof>Journal of agricultural and food chemistry, 2002-04, Vol.50 (8), p.2214-2219</ispartof><rights>Copyright © 2002 American Chemical Society</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a414t-af5e1d31517b20ef97f8c94069cfeddde721f106ca7bee81771b89cf32ff80f43</citedby><cites>FETCH-LOGICAL-a414t-af5e1d31517b20ef97f8c94069cfeddde721f106ca7bee81771b89cf32ff80f43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf0110351$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf0110351$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13606854$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11929273$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>van Breemen, Richard B</creatorcontrib><creatorcontrib>Xu, Xiaoying</creatorcontrib><creatorcontrib>Viana, Marlos A</creatorcontrib><creatorcontrib>Chen, Longwen</creatorcontrib><creatorcontrib>Stacewicz-Sapuntzakis, Maria</creatorcontrib><creatorcontrib>Duncan, Claudine</creatorcontrib><creatorcontrib>Bowen, Phyllis E</creatorcontrib><creatorcontrib>Sharifi, Roohollah</creatorcontrib><title>Liquid Chromatography−Mass Spectrometry of cis- and all-trans-Lycopene in Human Serum and Prostate Tissue after Dietary Supplementation with Tomato Sauce</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Several epidemiological studies suggest a lower incidence of prostate cancer in men who routinely consume tomato products. Tomatoes are the primary dietary source of lycopene, which is among the most potent antioxidants of the carotenoids. Men with clinical stage T1 or T2 prostate adenocarcinoma were recruited (n = 32) and consumed tomato sauce based pasta dishes for 3 weeks (equivalent to 30 mg of lycopene per day) before radical prostectomy. Prostate tissue from needle biopsy just before intervention and prostectomy after supplementation from a subset of 11 subjects was evaluated for both total lycopene and lycopene geometrical isomer ratios. A gradient HPLC system using a C18 column with UV−vis absorbance detection was used to measure total lycopene. Because the absorbance detector was insufficiently sensitive, HPLC with a C30 column and positive ion atmospheric pressure chemical ionization mass spectrometric (LC-MS) detection was developed as a new assay to measure the ratio of lycopene cis/trans isomers in these samples. The limit of detection of the LC-MS method was determined to be 0.93 pmol of lycopene on-column, and a linear response was obtained over 3 orders of magnitude. Total lycopene in serum increased 2.0-fold from 35.6 to 69.9 μg/dL (from 0.664 to 1.30 μM) as a result of dietary supplementation with tomato sauce, whereas total lycopene in prostate tissue increased 3.0-fold from 0.196 to 0.582 ng/mg of tissue (from 0.365 to 1.09 pmol/mg). all-trans-Lycopene and at least 14 cis-isomer peaks were detected in prostate tissue and serum. The mean proportion of all-trans-lycopene in prostate tissue was ∼12.4% of total lycopene before supplementation but increased to 22.7% after dietary intervention with tomato sauce. In serum there was only a 2.8% but statistically significant increase in the proportion of all-trans-lycopene after intervention. These results indicate that short-term supplementation with tomato sauce containing primarily all-trans-lycopene (83% of total lycopene) results in substantial increases in total lycopene in serum and prostate and a substantial increase in all-trans-lycopene in prostate but relatively less in serum. Keywords: cis- and all-trans-lycopene; LC-MS; APCI; prostate cancer; tomato sauce; dietary supplement</description><subject>Adenocarcinoma - metabolism</subject><subject>Adenocarcinoma - surgery</subject><subject>Aged</subject><subject>Biological and medical sciences</subject><subject>Biopsy, Needle</subject><subject>Carcinogenesis, carcinogens and anticarcinogens</subject><subject>Carotenoids - administration & dosage</subject><subject>Carotenoids - analysis</subject><subject>Carotenoids - blood</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Chromatography, Liquid</subject><subject>Diet</subject><subject>Foods and miscellaneous</subject><subject>Humans</subject><subject>Isomerism</subject><subject>Lycopene</subject><subject>Lycopersicon esculentum</subject><subject>Male</subject><subject>Mass Spectrometry</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Prostate - chemistry</subject><subject>Prostatectomy</subject><subject>Prostatic Neoplasms - metabolism</subject><subject>Prostatic Neoplasms - surgery</subject><subject>Tumors</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkb2O1DAUhS0EYoeFghdAbkCiCPgmcZyUaPhZYBArEpptLI9zzXjI39qOYN6Ampa340nwzox2GqpbnE_n3nsOIY-BvQCWwsutYQAs43CHLICnLOEA5V2yYFFMSl7AGXng_ZYxVnLB7pMzgCqtUpEtyJ-VvZ5tS5cbN_YqjN-cmja7v79-f1Le03pCHaKAwe3oaKi2PqFqaKnquiQ4NfhktdPjhANSO9CLuVcDrdHN_Z66dKMPKiBtrPczUmUCOvraYlDRr56nqcMeh4jYcaA_bNjQZn8FrdWs8SG5Z1Tn8dFxnpOvb980y4tk9fnd--WrVaJyyEOiDEdoM-Ag1ilDUwlT6ipnRaUNtm2LIgUDrNBKrBFLEALWZdSy1JiSmTw7J88OvpMbr2f0QfbWa-w6NeA4eymAFzzjN-DzA6jjY96hkZOzffxFApM3TcjbJiL75Gg6r3tsT-Qx-gg8PQLKa9WZmGaM98RlBSvK_dLkwFkf8Oetrtx3WYhMcNlc1vKKffkITX4lP5x8lfZyO85uiNn958B_of2vUg</recordid><startdate>20020410</startdate><enddate>20020410</enddate><creator>van Breemen, Richard B</creator><creator>Xu, Xiaoying</creator><creator>Viana, Marlos A</creator><creator>Chen, Longwen</creator><creator>Stacewicz-Sapuntzakis, Maria</creator><creator>Duncan, Claudine</creator><creator>Bowen, Phyllis E</creator><creator>Sharifi, Roohollah</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020410</creationdate><title>Liquid Chromatography−Mass Spectrometry of cis- and all-trans-Lycopene in Human Serum and Prostate Tissue after Dietary Supplementation with Tomato Sauce</title><author>van Breemen, Richard B ; Xu, Xiaoying ; Viana, Marlos A ; Chen, Longwen ; Stacewicz-Sapuntzakis, Maria ; Duncan, Claudine ; Bowen, Phyllis E ; Sharifi, Roohollah</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a414t-af5e1d31517b20ef97f8c94069cfeddde721f106ca7bee81771b89cf32ff80f43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adenocarcinoma - metabolism</topic><topic>Adenocarcinoma - surgery</topic><topic>Aged</topic><topic>Biological and medical sciences</topic><topic>Biopsy, Needle</topic><topic>Carcinogenesis, carcinogens and anticarcinogens</topic><topic>Carotenoids - administration & dosage</topic><topic>Carotenoids - analysis</topic><topic>Carotenoids - blood</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Chromatography, Liquid</topic><topic>Diet</topic><topic>Foods and miscellaneous</topic><topic>Humans</topic><topic>Isomerism</topic><topic>Lycopene</topic><topic>Lycopersicon esculentum</topic><topic>Male</topic><topic>Mass Spectrometry</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Prostate - chemistry</topic><topic>Prostatectomy</topic><topic>Prostatic Neoplasms - metabolism</topic><topic>Prostatic Neoplasms - surgery</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>van Breemen, Richard B</creatorcontrib><creatorcontrib>Xu, Xiaoying</creatorcontrib><creatorcontrib>Viana, Marlos A</creatorcontrib><creatorcontrib>Chen, Longwen</creatorcontrib><creatorcontrib>Stacewicz-Sapuntzakis, Maria</creatorcontrib><creatorcontrib>Duncan, Claudine</creatorcontrib><creatorcontrib>Bowen, Phyllis E</creatorcontrib><creatorcontrib>Sharifi, Roohollah</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>van Breemen, Richard B</au><au>Xu, Xiaoying</au><au>Viana, Marlos A</au><au>Chen, Longwen</au><au>Stacewicz-Sapuntzakis, Maria</au><au>Duncan, Claudine</au><au>Bowen, Phyllis E</au><au>Sharifi, Roohollah</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Liquid Chromatography−Mass Spectrometry of cis- and all-trans-Lycopene in Human Serum and Prostate Tissue after Dietary Supplementation with Tomato Sauce</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2002-04-10</date><risdate>2002</risdate><volume>50</volume><issue>8</issue><spage>2214</spage><epage>2219</epage><pages>2214-2219</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>Several epidemiological studies suggest a lower incidence of prostate cancer in men who routinely consume tomato products. Tomatoes are the primary dietary source of lycopene, which is among the most potent antioxidants of the carotenoids. Men with clinical stage T1 or T2 prostate adenocarcinoma were recruited (n = 32) and consumed tomato sauce based pasta dishes for 3 weeks (equivalent to 30 mg of lycopene per day) before radical prostectomy. Prostate tissue from needle biopsy just before intervention and prostectomy after supplementation from a subset of 11 subjects was evaluated for both total lycopene and lycopene geometrical isomer ratios. A gradient HPLC system using a C18 column with UV−vis absorbance detection was used to measure total lycopene. Because the absorbance detector was insufficiently sensitive, HPLC with a C30 column and positive ion atmospheric pressure chemical ionization mass spectrometric (LC-MS) detection was developed as a new assay to measure the ratio of lycopene cis/trans isomers in these samples. The limit of detection of the LC-MS method was determined to be 0.93 pmol of lycopene on-column, and a linear response was obtained over 3 orders of magnitude. Total lycopene in serum increased 2.0-fold from 35.6 to 69.9 μg/dL (from 0.664 to 1.30 μM) as a result of dietary supplementation with tomato sauce, whereas total lycopene in prostate tissue increased 3.0-fold from 0.196 to 0.582 ng/mg of tissue (from 0.365 to 1.09 pmol/mg). all-trans-Lycopene and at least 14 cis-isomer peaks were detected in prostate tissue and serum. The mean proportion of all-trans-lycopene in prostate tissue was ∼12.4% of total lycopene before supplementation but increased to 22.7% after dietary intervention with tomato sauce. In serum there was only a 2.8% but statistically significant increase in the proportion of all-trans-lycopene after intervention. These results indicate that short-term supplementation with tomato sauce containing primarily all-trans-lycopene (83% of total lycopene) results in substantial increases in total lycopene in serum and prostate and a substantial increase in all-trans-lycopene in prostate but relatively less in serum. Keywords: cis- and all-trans-lycopene; LC-MS; APCI; prostate cancer; tomato sauce; dietary supplement</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>11929273</pmid><doi>10.1021/jf0110351</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of agricultural and food chemistry, 2002-04, Vol.50 (8), p.2214-2219 |
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| source | ACS Publications; MEDLINE |
| subjects | Adenocarcinoma - metabolism Adenocarcinoma - surgery Aged Biological and medical sciences Biopsy, Needle Carcinogenesis, carcinogens and anticarcinogens Carotenoids - administration & dosage Carotenoids - analysis Carotenoids - blood Chromatography, High Pressure Liquid Chromatography, Liquid Diet Foods and miscellaneous Humans Isomerism Lycopene Lycopersicon esculentum Male Mass Spectrometry Medical sciences Middle Aged Prostate - chemistry Prostatectomy Prostatic Neoplasms - metabolism Prostatic Neoplasms - surgery Tumors |
| title | Liquid Chromatography−Mass Spectrometry of cis- and all-trans-Lycopene in Human Serum and Prostate Tissue after Dietary Supplementation with Tomato Sauce |
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