Enhancement of stability of immobilized glucose oxidase by modification of free thiols generated by reducing disulfide bonds and using additives
Stability of glucose oxidase (GOD) immobilized with lysozyme has been considerably enhanced by modification of free thiols generated by reducing disulfide bonds using β-mercaptoethanol and N-ethylmaleimide in conjunction with additives like antibiotics and salts. Thermal stability of immobilized GOD...
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| Veröffentlicht in: | Biosensors & bioelectronics 2004-01, Vol.19 (6), p.621-625 |
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| container_title | Biosensors & bioelectronics |
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| creator | Gulla, K.C. Gouda, M.D. Thakur, M.S. Karanth, N.G. |
| description | Stability of glucose oxidase (GOD) immobilized with lysozyme has been considerably enhanced by modification of free thiols generated by reducing disulfide bonds using β-mercaptoethanol and
N-ethylmaleimide in conjunction with additives like antibiotics and salts. Thermal stability of immobilized GOD was quantified by means of the transition temperature,
T
m and the operational stability by half-life
t
1/2 at 70
°C. Modification of the free thiols in the enzyme coupled with the presence of kanamycin, NaCl, and K
2SO
4, led to increase in
T
m, to 80, 82 and 84
°C (compared to 75
°C in control) and
t
1/2 by 7.7-, 11- and 22-fold, respectively, indicating that this method can be effectively used for enhancing the stability of enzymes. |
| doi_str_mv | 10.1016/S0956-5663(03)00258-6 |
| format | Article |
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N-ethylmaleimide in conjunction with additives like antibiotics and salts. Thermal stability of immobilized GOD was quantified by means of the transition temperature,
T
m and the operational stability by half-life
t
1/2 at 70
°C. Modification of the free thiols in the enzyme coupled with the presence of kanamycin, NaCl, and K
2SO
4, led to increase in
T
m, to 80, 82 and 84
°C (compared to 75
°C in control) and
t
1/2 by 7.7-, 11- and 22-fold, respectively, indicating that this method can be effectively used for enhancing the stability of enzymes.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/S0956-5663(03)00258-6</identifier><identifier>PMID: 14683646</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Additives ; Biological and medical sciences ; Biosensing Techniques - instrumentation ; Biosensing Techniques - methods ; Biosensor ; Biosensors ; Biotechnology ; Coated Materials, Biocompatible - chemical synthesis ; Coated Materials, Biocompatible - chemistry ; Disulfides - chemistry ; Electrodes ; Enzyme Stability ; Enzymes, Immobilized - chemistry ; Equipment Design ; Equipment Failure Analysis ; Free Radicals - chemistry ; Fundamental and applied biological sciences. Psychology ; Glucose oxidase ; Glucose Oxidase - chemistry ; Methods. Procedures. Technologies ; Oxidation-Reduction ; Reproducibility of Results ; Sensitivity and Specificity ; Sulfhydryl Compounds - chemistry ; Temperature ; Thermal stability ; Thiol group modification ; Various methods and equipments</subject><ispartof>Biosensors & bioelectronics, 2004-01, Vol.19 (6), p.621-625</ispartof><rights>2003 Elsevier B.V.</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c488t-910b05563fe725d7d2eb85eaa5778e60526bfd5440040146783963110bf9e9593</citedby><cites>FETCH-LOGICAL-c488t-910b05563fe725d7d2eb85eaa5778e60526bfd5440040146783963110bf9e9593</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0956-5663(03)00258-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15475003$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14683646$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gulla, K.C.</creatorcontrib><creatorcontrib>Gouda, M.D.</creatorcontrib><creatorcontrib>Thakur, M.S.</creatorcontrib><creatorcontrib>Karanth, N.G.</creatorcontrib><title>Enhancement of stability of immobilized glucose oxidase by modification of free thiols generated by reducing disulfide bonds and using additives</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>Stability of glucose oxidase (GOD) immobilized with lysozyme has been considerably enhanced by modification of free thiols generated by reducing disulfide bonds using β-mercaptoethanol and
N-ethylmaleimide in conjunction with additives like antibiotics and salts. Thermal stability of immobilized GOD was quantified by means of the transition temperature,
T
m and the operational stability by half-life
t
1/2 at 70
°C. Modification of the free thiols in the enzyme coupled with the presence of kanamycin, NaCl, and K
2SO
4, led to increase in
T
m, to 80, 82 and 84
°C (compared to 75
°C in control) and
t
1/2 by 7.7-, 11- and 22-fold, respectively, indicating that this method can be effectively used for enhancing the stability of enzymes.</description><subject>Additives</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques - instrumentation</subject><subject>Biosensing Techniques - methods</subject><subject>Biosensor</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Coated Materials, Biocompatible - chemical synthesis</subject><subject>Coated Materials, Biocompatible - chemistry</subject><subject>Disulfides - chemistry</subject><subject>Electrodes</subject><subject>Enzyme Stability</subject><subject>Enzymes, Immobilized - chemistry</subject><subject>Equipment Design</subject><subject>Equipment Failure Analysis</subject><subject>Free Radicals - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucose oxidase</subject><subject>Glucose Oxidase - chemistry</subject><subject>Methods. Procedures. Technologies</subject><subject>Oxidation-Reduction</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Sulfhydryl Compounds - chemistry</subject><subject>Temperature</subject><subject>Thermal stability</subject><subject>Thiol group modification</subject><subject>Various methods and equipments</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1rFTEUhoMotlZ_gpKNoovRk8kkmVkVKfUDCi7UdcgkJ7eRmUlNMsXrr_Anm_Fe7LIQOBzyvCcfDyHPGbxlwOS7rzAI2Qgp-WvgbwBa0TfyATllveJN13LxkJz-R07Ik5x_AIBiAzwmJ6yTPZedPCV_Lpdrs1iccSk0epqLGcMUyn5rwjzHrfuNju6m1caMNP4KztQ67ukcXfDBmhLisuE-IdJyHeKU6Q4XTKbUYAUTutWGZUddyOvkg6vxuLhMzeLomrcd41wo4RbzU_LImynjs2M9I98_XH67-NRcffn4-eL9VWO7vi_NwGAEIST3qFrhlGtx7AUaI5TqUYJo5eid6DqADupzVc8HyVlN-QEHMfAz8uow9ybFnyvmoueQLU6TWTCuWSsmpKiBe0E2tDAouU0UB9CmmHNCr29SmE3aawZ6c6b_OdObEA11bc60rLkXxwPWcUZ3lzpKqsDLI2CyNZNPVVjId5zolADglTs_cFj_7TZg0tkGrHJdSGiLdjHcc5W_6QW05g</recordid><startdate>20040115</startdate><enddate>20040115</enddate><creator>Gulla, K.C.</creator><creator>Gouda, M.D.</creator><creator>Thakur, M.S.</creator><creator>Karanth, N.G.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20040115</creationdate><title>Enhancement of stability of immobilized glucose oxidase by modification of free thiols generated by reducing disulfide bonds and using additives</title><author>Gulla, K.C. ; Gouda, M.D. ; Thakur, M.S. ; Karanth, N.G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c488t-910b05563fe725d7d2eb85eaa5778e60526bfd5440040146783963110bf9e9593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Additives</topic><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - instrumentation</topic><topic>Biosensing Techniques - methods</topic><topic>Biosensor</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Coated Materials, Biocompatible - chemical synthesis</topic><topic>Coated Materials, Biocompatible - chemistry</topic><topic>Disulfides - chemistry</topic><topic>Electrodes</topic><topic>Enzyme Stability</topic><topic>Enzymes, Immobilized - chemistry</topic><topic>Equipment Design</topic><topic>Equipment Failure Analysis</topic><topic>Free Radicals - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucose oxidase</topic><topic>Glucose Oxidase - chemistry</topic><topic>Methods. Procedures. Technologies</topic><topic>Oxidation-Reduction</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Sulfhydryl Compounds - chemistry</topic><topic>Temperature</topic><topic>Thermal stability</topic><topic>Thiol group modification</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gulla, K.C.</creatorcontrib><creatorcontrib>Gouda, M.D.</creatorcontrib><creatorcontrib>Thakur, M.S.</creatorcontrib><creatorcontrib>Karanth, N.G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gulla, K.C.</au><au>Gouda, M.D.</au><au>Thakur, M.S.</au><au>Karanth, N.G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhancement of stability of immobilized glucose oxidase by modification of free thiols generated by reducing disulfide bonds and using additives</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2004-01-15</date><risdate>2004</risdate><volume>19</volume><issue>6</issue><spage>621</spage><epage>625</epage><pages>621-625</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>Stability of glucose oxidase (GOD) immobilized with lysozyme has been considerably enhanced by modification of free thiols generated by reducing disulfide bonds using β-mercaptoethanol and
N-ethylmaleimide in conjunction with additives like antibiotics and salts. Thermal stability of immobilized GOD was quantified by means of the transition temperature,
T
m and the operational stability by half-life
t
1/2 at 70
°C. Modification of the free thiols in the enzyme coupled with the presence of kanamycin, NaCl, and K
2SO
4, led to increase in
T
m, to 80, 82 and 84
°C (compared to 75
°C in control) and
t
1/2 by 7.7-, 11- and 22-fold, respectively, indicating that this method can be effectively used for enhancing the stability of enzymes.</abstract><cop>Lausanne</cop><pub>Elsevier B.V</pub><pmid>14683646</pmid><doi>10.1016/S0956-5663(03)00258-6</doi><tpages>5</tpages></addata></record> |
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| ispartof | Biosensors & bioelectronics, 2004-01, Vol.19 (6), p.621-625 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Additives Biological and medical sciences Biosensing Techniques - instrumentation Biosensing Techniques - methods Biosensor Biosensors Biotechnology Coated Materials, Biocompatible - chemical synthesis Coated Materials, Biocompatible - chemistry Disulfides - chemistry Electrodes Enzyme Stability Enzymes, Immobilized - chemistry Equipment Design Equipment Failure Analysis Free Radicals - chemistry Fundamental and applied biological sciences. Psychology Glucose oxidase Glucose Oxidase - chemistry Methods. Procedures. Technologies Oxidation-Reduction Reproducibility of Results Sensitivity and Specificity Sulfhydryl Compounds - chemistry Temperature Thermal stability Thiol group modification Various methods and equipments |
| title | Enhancement of stability of immobilized glucose oxidase by modification of free thiols generated by reducing disulfide bonds and using additives |
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