Comparison of in vitro closure time (PFA-100) with whole blood electrical aggregometry and platelet surface antigen expression in healthy volunteers
It was the aim of this study to compare in vitro closure time (PFA-100), reflecting platelet-related primary hemostasis, to more platelet-specific tests like whole blood electrical aggregometry and platelet surface antigen expression in healthy volunteers. In vitro closure time was measured using a...
Gespeichert in:
| Veröffentlicht in: | Thrombosis research 2002-02, Vol.105 (3), p.205-208 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 208 |
|---|---|
| container_issue | 3 |
| container_start_page | 205 |
| container_title | Thrombosis research |
| container_volume | 105 |
| creator | Salat, Andreas Kroess, Sabine Felfernig-Boehm, Dagmar Felfernig, Michael Fleck, Tatjana Schmidt, Daniela Pulaki, Sad Mueller, Michael R. |
| description | It was the aim of this study to compare in vitro closure time (PFA-100), reflecting platelet-related primary hemostasis, to more platelet-specific tests like whole blood electrical aggregometry and platelet surface antigen expression in healthy volunteers. In vitro closure time was measured using a PFA-100. Platelet surface antigen expression (CD63, CD62-P, CD42b, CD36, CD31) was determined in accordance with the consensus protocol for flow-cytometric characterisation of platelet function. Platelet aggregometry was performed using a whole blood electrical aggregometer (ADP and arachidonic acid as agonists). Analysis of the obtained data revealed only a few significant correlations between the different platelet function tests used. This finding can be explained by the various aspects of platelet function being focused by these tests in different extents. Whenever platelet function is analysed, the investigator should be aware of the specific and limited evidence of the method used. For screening purposes, it may be useful to introduce a platelet function index, referring to basal platelet activity, platelet adhesion and platelet aggregation at low and high shear stress forces. |
| doi_str_mv | 10.1016/S0049-3848(02)00019-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71561386</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0049384802000191</els_id><sourcerecordid>71561386</sourcerecordid><originalsourceid>FETCH-LOGICAL-c391t-d253ca0985017c8d29ae2aac1c80e347ec2164997d645292234d6a3738c6c8f93</originalsourceid><addsrcrecordid>eNqFkcFu1DAQhi0EotvCI4B8AbWHgMdO4viEqlULSJVAAs6W60x2jZw42M6WfQ8eGLe7okdOI3m-mX_8_4S8AvYOGLTvvzFWq0p0dXfO-AVjDFQFT8gKOqkqXkv-lKz-ISfkNKWfhZGgmufkBEBxCbxZkT_rMM4muhQmGgbqJrpzOQZqfUhLRJrdiPT86_VlBYxd0DuXt_RuGzzSWx9CT9GjzdFZ46nZbCJuwog57qmZejp7k0s_07JpMBbLY3YbnCj-niOm5IpmEdyi8Xm7p7vglykjxvSCPBuMT_jyWM_Ij-ur7-tP1c2Xj5_XlzeVFQpy1fNGWMNU15SP2a7nyiA3xoLtGIpaouXQ1krJvq0brjgXdd8aIUVnW9sNSpyRt4e9cwy_FkxZjy5Z9N5MGJakJTQtiK4tYHMAbQwpRRz0HN1o4l4D0_dx6Ic49L3XmnH9EIeGMvf6KLDcjtg_Th39L8CbI2BS8XCIZrIuPXJCgmgUK9yHA4fFjp3DqJN1OFnsXSwB6D64_5zyF5qUqF0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71561386</pqid></control><display><type>article</type><title>Comparison of in vitro closure time (PFA-100) with whole blood electrical aggregometry and platelet surface antigen expression in healthy volunteers</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Salat, Andreas ; Kroess, Sabine ; Felfernig-Boehm, Dagmar ; Felfernig, Michael ; Fleck, Tatjana ; Schmidt, Daniela ; Pulaki, Sad ; Mueller, Michael R.</creator><creatorcontrib>Salat, Andreas ; Kroess, Sabine ; Felfernig-Boehm, Dagmar ; Felfernig, Michael ; Fleck, Tatjana ; Schmidt, Daniela ; Pulaki, Sad ; Mueller, Michael R.</creatorcontrib><description>It was the aim of this study to compare in vitro closure time (PFA-100), reflecting platelet-related primary hemostasis, to more platelet-specific tests like whole blood electrical aggregometry and platelet surface antigen expression in healthy volunteers. In vitro closure time was measured using a PFA-100. Platelet surface antigen expression (CD63, CD62-P, CD42b, CD36, CD31) was determined in accordance with the consensus protocol for flow-cytometric characterisation of platelet function. Platelet aggregometry was performed using a whole blood electrical aggregometer (ADP and arachidonic acid as agonists). Analysis of the obtained data revealed only a few significant correlations between the different platelet function tests used. This finding can be explained by the various aspects of platelet function being focused by these tests in different extents. Whenever platelet function is analysed, the investigator should be aware of the specific and limited evidence of the method used. For screening purposes, it may be useful to introduce a platelet function index, referring to basal platelet activity, platelet adhesion and platelet aggregation at low and high shear stress forces.</description><identifier>ISSN: 0049-3848</identifier><identifier>EISSN: 1879-2472</identifier><identifier>DOI: 10.1016/S0049-3848(02)00019-1</identifier><identifier>PMID: 11927125</identifier><identifier>CODEN: THBRAA</identifier><language>eng</language><publisher>New York, NY: Elsevier Ltd</publisher><subject>Aggregometry ; Antigens, CD - analysis ; Antigens, CD - immunology ; Biological and medical sciences ; Blood coagulation ; Blood Platelets - immunology ; Flow cytometry ; Hemostasis ; Humans ; In vitro closure time ; Investigative techniques, diagnostic techniques (general aspects) ; Medical sciences ; Platelet Aggregation ; Platelet Function Tests ; Platelets ; Time Factors</subject><ispartof>Thrombosis research, 2002-02, Vol.105 (3), p.205-208</ispartof><rights>2002 Elsevier Science Ltd</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-d253ca0985017c8d29ae2aac1c80e347ec2164997d645292234d6a3738c6c8f93</citedby><cites>FETCH-LOGICAL-c391t-d253ca0985017c8d29ae2aac1c80e347ec2164997d645292234d6a3738c6c8f93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0049-3848(02)00019-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13713590$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11927125$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Salat, Andreas</creatorcontrib><creatorcontrib>Kroess, Sabine</creatorcontrib><creatorcontrib>Felfernig-Boehm, Dagmar</creatorcontrib><creatorcontrib>Felfernig, Michael</creatorcontrib><creatorcontrib>Fleck, Tatjana</creatorcontrib><creatorcontrib>Schmidt, Daniela</creatorcontrib><creatorcontrib>Pulaki, Sad</creatorcontrib><creatorcontrib>Mueller, Michael R.</creatorcontrib><title>Comparison of in vitro closure time (PFA-100) with whole blood electrical aggregometry and platelet surface antigen expression in healthy volunteers</title><title>Thrombosis research</title><addtitle>Thromb Res</addtitle><description>It was the aim of this study to compare in vitro closure time (PFA-100), reflecting platelet-related primary hemostasis, to more platelet-specific tests like whole blood electrical aggregometry and platelet surface antigen expression in healthy volunteers. In vitro closure time was measured using a PFA-100. Platelet surface antigen expression (CD63, CD62-P, CD42b, CD36, CD31) was determined in accordance with the consensus protocol for flow-cytometric characterisation of platelet function. Platelet aggregometry was performed using a whole blood electrical aggregometer (ADP and arachidonic acid as agonists). Analysis of the obtained data revealed only a few significant correlations between the different platelet function tests used. This finding can be explained by the various aspects of platelet function being focused by these tests in different extents. Whenever platelet function is analysed, the investigator should be aware of the specific and limited evidence of the method used. For screening purposes, it may be useful to introduce a platelet function index, referring to basal platelet activity, platelet adhesion and platelet aggregation at low and high shear stress forces.</description><subject>Aggregometry</subject><subject>Antigens, CD - analysis</subject><subject>Antigens, CD - immunology</subject><subject>Biological and medical sciences</subject><subject>Blood coagulation</subject><subject>Blood Platelets - immunology</subject><subject>Flow cytometry</subject><subject>Hemostasis</subject><subject>Humans</subject><subject>In vitro closure time</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Medical sciences</subject><subject>Platelet Aggregation</subject><subject>Platelet Function Tests</subject><subject>Platelets</subject><subject>Time Factors</subject><issn>0049-3848</issn><issn>1879-2472</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhi0EotvCI4B8AbWHgMdO4viEqlULSJVAAs6W60x2jZw42M6WfQ8eGLe7okdOI3m-mX_8_4S8AvYOGLTvvzFWq0p0dXfO-AVjDFQFT8gKOqkqXkv-lKz-ISfkNKWfhZGgmufkBEBxCbxZkT_rMM4muhQmGgbqJrpzOQZqfUhLRJrdiPT86_VlBYxd0DuXt_RuGzzSWx9CT9GjzdFZ46nZbCJuwog57qmZejp7k0s_07JpMBbLY3YbnCj-niOm5IpmEdyi8Xm7p7vglykjxvSCPBuMT_jyWM_Ij-ur7-tP1c2Xj5_XlzeVFQpy1fNGWMNU15SP2a7nyiA3xoLtGIpaouXQ1krJvq0brjgXdd8aIUVnW9sNSpyRt4e9cwy_FkxZjy5Z9N5MGJakJTQtiK4tYHMAbQwpRRz0HN1o4l4D0_dx6Ic49L3XmnH9EIeGMvf6KLDcjtg_Th39L8CbI2BS8XCIZrIuPXJCgmgUK9yHA4fFjp3DqJN1OFnsXSwB6D64_5zyF5qUqF0</recordid><startdate>20020201</startdate><enddate>20020201</enddate><creator>Salat, Andreas</creator><creator>Kroess, Sabine</creator><creator>Felfernig-Boehm, Dagmar</creator><creator>Felfernig, Michael</creator><creator>Fleck, Tatjana</creator><creator>Schmidt, Daniela</creator><creator>Pulaki, Sad</creator><creator>Mueller, Michael R.</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020201</creationdate><title>Comparison of in vitro closure time (PFA-100) with whole blood electrical aggregometry and platelet surface antigen expression in healthy volunteers</title><author>Salat, Andreas ; Kroess, Sabine ; Felfernig-Boehm, Dagmar ; Felfernig, Michael ; Fleck, Tatjana ; Schmidt, Daniela ; Pulaki, Sad ; Mueller, Michael R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-d253ca0985017c8d29ae2aac1c80e347ec2164997d645292234d6a3738c6c8f93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Aggregometry</topic><topic>Antigens, CD - analysis</topic><topic>Antigens, CD - immunology</topic><topic>Biological and medical sciences</topic><topic>Blood coagulation</topic><topic>Blood Platelets - immunology</topic><topic>Flow cytometry</topic><topic>Hemostasis</topic><topic>Humans</topic><topic>In vitro closure time</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Medical sciences</topic><topic>Platelet Aggregation</topic><topic>Platelet Function Tests</topic><topic>Platelets</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Salat, Andreas</creatorcontrib><creatorcontrib>Kroess, Sabine</creatorcontrib><creatorcontrib>Felfernig-Boehm, Dagmar</creatorcontrib><creatorcontrib>Felfernig, Michael</creatorcontrib><creatorcontrib>Fleck, Tatjana</creatorcontrib><creatorcontrib>Schmidt, Daniela</creatorcontrib><creatorcontrib>Pulaki, Sad</creatorcontrib><creatorcontrib>Mueller, Michael R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Thrombosis research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Salat, Andreas</au><au>Kroess, Sabine</au><au>Felfernig-Boehm, Dagmar</au><au>Felfernig, Michael</au><au>Fleck, Tatjana</au><au>Schmidt, Daniela</au><au>Pulaki, Sad</au><au>Mueller, Michael R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of in vitro closure time (PFA-100) with whole blood electrical aggregometry and platelet surface antigen expression in healthy volunteers</atitle><jtitle>Thrombosis research</jtitle><addtitle>Thromb Res</addtitle><date>2002-02-01</date><risdate>2002</risdate><volume>105</volume><issue>3</issue><spage>205</spage><epage>208</epage><pages>205-208</pages><issn>0049-3848</issn><eissn>1879-2472</eissn><coden>THBRAA</coden><abstract>It was the aim of this study to compare in vitro closure time (PFA-100), reflecting platelet-related primary hemostasis, to more platelet-specific tests like whole blood electrical aggregometry and platelet surface antigen expression in healthy volunteers. In vitro closure time was measured using a PFA-100. Platelet surface antigen expression (CD63, CD62-P, CD42b, CD36, CD31) was determined in accordance with the consensus protocol for flow-cytometric characterisation of platelet function. Platelet aggregometry was performed using a whole blood electrical aggregometer (ADP and arachidonic acid as agonists). Analysis of the obtained data revealed only a few significant correlations between the different platelet function tests used. This finding can be explained by the various aspects of platelet function being focused by these tests in different extents. Whenever platelet function is analysed, the investigator should be aware of the specific and limited evidence of the method used. For screening purposes, it may be useful to introduce a platelet function index, referring to basal platelet activity, platelet adhesion and platelet aggregation at low and high shear stress forces.</abstract><cop>New York, NY</cop><pub>Elsevier Ltd</pub><pmid>11927125</pmid><doi>10.1016/S0049-3848(02)00019-1</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0049-3848 |
| ispartof | Thrombosis research, 2002-02, Vol.105 (3), p.205-208 |
| issn | 0049-3848 1879-2472 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71561386 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Aggregometry Antigens, CD - analysis Antigens, CD - immunology Biological and medical sciences Blood coagulation Blood Platelets - immunology Flow cytometry Hemostasis Humans In vitro closure time Investigative techniques, diagnostic techniques (general aspects) Medical sciences Platelet Aggregation Platelet Function Tests Platelets Time Factors |
| title | Comparison of in vitro closure time (PFA-100) with whole blood electrical aggregometry and platelet surface antigen expression in healthy volunteers |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T03%3A43%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparison%20of%20in%20vitro%20closure%20time%20(PFA-100)%20with%20whole%20blood%20electrical%20aggregometry%20and%20platelet%20surface%20antigen%20expression%20in%20healthy%20volunteers&rft.jtitle=Thrombosis%20research&rft.au=Salat,%20Andreas&rft.date=2002-02-01&rft.volume=105&rft.issue=3&rft.spage=205&rft.epage=208&rft.pages=205-208&rft.issn=0049-3848&rft.eissn=1879-2472&rft.coden=THBRAA&rft_id=info:doi/10.1016/S0049-3848(02)00019-1&rft_dat=%3Cproquest_cross%3E71561386%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=71561386&rft_id=info:pmid/11927125&rft_els_id=S0049384802000191&rfr_iscdi=true |