Immobilization of chitosan onto poly- l-lactic acid film surface by plasma graft polymerization to control the morphology of fibroblast and liver cells
Surface functionalization of biodegradable poly- l-lactic acid (PLLA) was achieved by plasma coupling reaction of chitosan. The structure of modified PLLA surfaces was characterized by contact angle measurements and X-ray photoelectron spectroscopy. Two cell lines, L929 (mouse fibroblasts) and L02 (...
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| Veröffentlicht in: | Biomaterials 2004-03, Vol.25 (6), p.1059-1067 |
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| creator | Ding, Zhi Chen, Jiangning Gao, Shuying Chang, Jianbing Zhang, Junfeng Kang, E.T. |
| description | Surface functionalization of biodegradable poly-
l-lactic acid (PLLA) was achieved by plasma coupling reaction of chitosan. The structure of modified PLLA surfaces was characterized by contact angle measurements and X-ray photoelectron spectroscopy. Two cell lines, L929 (mouse fibroblasts) and L02 (human hepatocytes), were cultured on the modified PLLA surface. It was found that cells cultured on this film could hardly spread and tend to become round, and the film was demonstrated to be a poorly adhering substrate. However, cells grown on this substrate can proliferate at almost the same speed as cultured on a glass surface. These results suggest that the new substrate can be used to control the morphology of cells, and has potential applications in tissue engineering. It may be helpful in understanding the mechanism of the switch between cell phases of growth and differentiation, which is necessary for the design of tissue regeneration biomaterials. |
| doi_str_mv | 10.1016/S0142-9612(03)00615-X |
| format | Article |
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l-lactic acid (PLLA) was achieved by plasma coupling reaction of chitosan. The structure of modified PLLA surfaces was characterized by contact angle measurements and X-ray photoelectron spectroscopy. Two cell lines, L929 (mouse fibroblasts) and L02 (human hepatocytes), were cultured on the modified PLLA surface. It was found that cells cultured on this film could hardly spread and tend to become round, and the film was demonstrated to be a poorly adhering substrate. However, cells grown on this substrate can proliferate at almost the same speed as cultured on a glass surface. These results suggest that the new substrate can be used to control the morphology of cells, and has potential applications in tissue engineering. It may be helpful in understanding the mechanism of the switch between cell phases of growth and differentiation, which is necessary for the design of tissue regeneration biomaterials.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/S0142-9612(03)00615-X</identifier><identifier>PMID: 14615171</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Adsorption ; Animals ; Cell culture ; Cell Differentiation - physiology ; Cell Line ; Cell Size - physiology ; Chitin - analogs & derivatives ; Chitin - chemistry ; Chitosan ; Coated Materials, Biocompatible - chemical synthesis ; Coated Materials, Biocompatible - chemistry ; Crystallization - methods ; Fibroblasts - cytology ; Fibroblasts - physiology ; Hepatocytes - cytology ; Hepatocytes - physiology ; Hot Temperature ; Humans ; Lactic Acid - chemistry ; Materials Testing ; Mice ; Molecular Conformation ; Plasma polymerization ; Poly- l-lactic acid ; Polyesters ; Polymers - chemical synthesis ; Polymers - chemistry ; Surface Properties ; Tissue Engineering - methods</subject><ispartof>Biomaterials, 2004-03, Vol.25 (6), p.1059-1067</ispartof><rights>2003 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c489t-18a77fc2b65ba31953570453aed99175264943e6c26f2ffc270609c40dfb95723</citedby><cites>FETCH-LOGICAL-c489t-18a77fc2b65ba31953570453aed99175264943e6c26f2ffc270609c40dfb95723</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S014296120300615X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14615171$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ding, Zhi</creatorcontrib><creatorcontrib>Chen, Jiangning</creatorcontrib><creatorcontrib>Gao, Shuying</creatorcontrib><creatorcontrib>Chang, Jianbing</creatorcontrib><creatorcontrib>Zhang, Junfeng</creatorcontrib><creatorcontrib>Kang, E.T.</creatorcontrib><title>Immobilization of chitosan onto poly- l-lactic acid film surface by plasma graft polymerization to control the morphology of fibroblast and liver cells</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Surface functionalization of biodegradable poly-
l-lactic acid (PLLA) was achieved by plasma coupling reaction of chitosan. The structure of modified PLLA surfaces was characterized by contact angle measurements and X-ray photoelectron spectroscopy. Two cell lines, L929 (mouse fibroblasts) and L02 (human hepatocytes), were cultured on the modified PLLA surface. It was found that cells cultured on this film could hardly spread and tend to become round, and the film was demonstrated to be a poorly adhering substrate. However, cells grown on this substrate can proliferate at almost the same speed as cultured on a glass surface. These results suggest that the new substrate can be used to control the morphology of cells, and has potential applications in tissue engineering. It may be helpful in understanding the mechanism of the switch between cell phases of growth and differentiation, which is necessary for the design of tissue regeneration biomaterials.</description><subject>Adsorption</subject><subject>Animals</subject><subject>Cell culture</subject><subject>Cell Differentiation - physiology</subject><subject>Cell Line</subject><subject>Cell Size - physiology</subject><subject>Chitin - analogs & derivatives</subject><subject>Chitin - chemistry</subject><subject>Chitosan</subject><subject>Coated Materials, Biocompatible - chemical synthesis</subject><subject>Coated Materials, Biocompatible - chemistry</subject><subject>Crystallization - methods</subject><subject>Fibroblasts - cytology</subject><subject>Fibroblasts - physiology</subject><subject>Hepatocytes - cytology</subject><subject>Hepatocytes - physiology</subject><subject>Hot Temperature</subject><subject>Humans</subject><subject>Lactic Acid - chemistry</subject><subject>Materials Testing</subject><subject>Mice</subject><subject>Molecular Conformation</subject><subject>Plasma polymerization</subject><subject>Poly- l-lactic acid</subject><subject>Polyesters</subject><subject>Polymers - chemical synthesis</subject><subject>Polymers - chemistry</subject><subject>Surface Properties</subject><subject>Tissue Engineering - methods</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUuLFDEUhYMoTjv6E5SsRBc1k3cqK5HBx8CAi1GYXUilkulIqlIm6YH2j_h3J_1Ql726ufCdcy45ALzG6AIjLC5vEWakUwKTd4i-R0hg3t09ASvcy77jCvGnYPUPOQMvSvmJ2o4YeQ7OMGs4lngF_lxPUxpCDL9NDWmGyUO7DjUV095zTXBJcdvB2EVja7DQ2DBCH-IEyyZ7Yx0ctnCJpkwG3mfj614wufzXsFnYZpRThHXt4JTysk4x3W93UT4MOQ1NXaGZRxjDg8vQuhjLS_DMm1jcq-M8Bz8-f_p-9bW7-fbl-urjTWdZr2qHeyOlt2QQfDAUK065RIxT40alsOREMMWoE5YIT3wDJRJIWYZGPyguCT0Hbw--S06_Nq5UPYWyu8DMLm2KlpgLRCU_CZKesJ5QeRLECitEqGggP4A2p1Ky83rJYTJ5qzHSu471vmO9K1Ajqvcd67ume3MM2AyTG_-rjqU24MMBcO3jHoLLutjgZuvGkJ2tekzhRMQjByy4ZQ</recordid><startdate>20040301</startdate><enddate>20040301</enddate><creator>Ding, Zhi</creator><creator>Chen, Jiangning</creator><creator>Gao, Shuying</creator><creator>Chang, Jianbing</creator><creator>Zhang, Junfeng</creator><creator>Kang, E.T.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>F28</scope><scope>7X8</scope></search><sort><creationdate>20040301</creationdate><title>Immobilization of chitosan onto poly- l-lactic acid film surface by plasma graft polymerization to control the morphology of fibroblast and liver cells</title><author>Ding, Zhi ; Chen, Jiangning ; Gao, Shuying ; Chang, Jianbing ; Zhang, Junfeng ; Kang, E.T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c489t-18a77fc2b65ba31953570453aed99175264943e6c26f2ffc270609c40dfb95723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adsorption</topic><topic>Animals</topic><topic>Cell culture</topic><topic>Cell Differentiation - physiology</topic><topic>Cell Line</topic><topic>Cell Size - physiology</topic><topic>Chitin - analogs & derivatives</topic><topic>Chitin - chemistry</topic><topic>Chitosan</topic><topic>Coated Materials, Biocompatible - chemical synthesis</topic><topic>Coated Materials, Biocompatible - chemistry</topic><topic>Crystallization - methods</topic><topic>Fibroblasts - cytology</topic><topic>Fibroblasts - physiology</topic><topic>Hepatocytes - cytology</topic><topic>Hepatocytes - physiology</topic><topic>Hot Temperature</topic><topic>Humans</topic><topic>Lactic Acid - chemistry</topic><topic>Materials Testing</topic><topic>Mice</topic><topic>Molecular Conformation</topic><topic>Plasma polymerization</topic><topic>Poly- l-lactic acid</topic><topic>Polyesters</topic><topic>Polymers - chemical synthesis</topic><topic>Polymers - chemistry</topic><topic>Surface Properties</topic><topic>Tissue Engineering - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ding, Zhi</creatorcontrib><creatorcontrib>Chen, Jiangning</creatorcontrib><creatorcontrib>Gao, Shuying</creatorcontrib><creatorcontrib>Chang, Jianbing</creatorcontrib><creatorcontrib>Zhang, Junfeng</creatorcontrib><creatorcontrib>Kang, E.T.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>MEDLINE - Academic</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ding, Zhi</au><au>Chen, Jiangning</au><au>Gao, Shuying</au><au>Chang, Jianbing</au><au>Zhang, Junfeng</au><au>Kang, E.T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immobilization of chitosan onto poly- l-lactic acid film surface by plasma graft polymerization to control the morphology of fibroblast and liver cells</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2004-03-01</date><risdate>2004</risdate><volume>25</volume><issue>6</issue><spage>1059</spage><epage>1067</epage><pages>1059-1067</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>Surface functionalization of biodegradable poly-
l-lactic acid (PLLA) was achieved by plasma coupling reaction of chitosan. The structure of modified PLLA surfaces was characterized by contact angle measurements and X-ray photoelectron spectroscopy. Two cell lines, L929 (mouse fibroblasts) and L02 (human hepatocytes), were cultured on the modified PLLA surface. It was found that cells cultured on this film could hardly spread and tend to become round, and the film was demonstrated to be a poorly adhering substrate. However, cells grown on this substrate can proliferate at almost the same speed as cultured on a glass surface. These results suggest that the new substrate can be used to control the morphology of cells, and has potential applications in tissue engineering. It may be helpful in understanding the mechanism of the switch between cell phases of growth and differentiation, which is necessary for the design of tissue regeneration biomaterials.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>14615171</pmid><doi>10.1016/S0142-9612(03)00615-X</doi><tpages>9</tpages></addata></record> |
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| subjects | Adsorption Animals Cell culture Cell Differentiation - physiology Cell Line Cell Size - physiology Chitin - analogs & derivatives Chitin - chemistry Chitosan Coated Materials, Biocompatible - chemical synthesis Coated Materials, Biocompatible - chemistry Crystallization - methods Fibroblasts - cytology Fibroblasts - physiology Hepatocytes - cytology Hepatocytes - physiology Hot Temperature Humans Lactic Acid - chemistry Materials Testing Mice Molecular Conformation Plasma polymerization Poly- l-lactic acid Polyesters Polymers - chemical synthesis Polymers - chemistry Surface Properties Tissue Engineering - methods |
| title | Immobilization of chitosan onto poly- l-lactic acid film surface by plasma graft polymerization to control the morphology of fibroblast and liver cells |
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