Immobilization of DNA Hydrogel Plugs in Microfluidic Channels

Acrylamide-modified DNA probes are immobilized in polycarbonate microfluidic channels via photopolymerization in a polyacrylamide matrix. The resulting polymeric, hydrogel plugs are porous under electrophoretic conditions and hybridize with fluorescently tagged complementary DNA. The double-stranded...

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Veröffentlicht in:	Analytical chemistry (Washington) 2002-03, Vol.74 (6), p.1436-1441
Hauptverfasser:	Olsen, Kimberly G, Ross, David J, Tarlov, Michael J
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biopolymers Chemistry Deoxyribonucleic acid Diverse techniques DNA DNA - chemistry Fluid dynamics Fluorescent Dyes Fundamental and applied biological sciences. Psychology Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry Molecular and cellular biology
Online-Zugang:	Volltext
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