Purification of antimicrobial factor from granules of channel catfish peripheral blood leucocytes
The channel catfish ( Ictalurus punctatus) is extensively used in aquaculture in the Southeast US and is susceptible to many bacterial infections acquired from its pond environment. Research is needed to better understand the defensive response and innate immunity of channel catfish against fish pat...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2004-01, Vol.313 (1), p.28-36 |
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| creator | Ourth, Donald D Chung, Kyung T |
| description | The channel catfish (
Ictalurus punctatus) is extensively used in aquaculture in the Southeast US and is susceptible to many bacterial infections acquired from its pond environment. Research is needed to better understand the defensive response and innate immunity of channel catfish against fish pathogens like
Edwardsiella ictaluri and
Aeromonas hydrophila. The main objectives were purification and characterization of an innate antimicrobial factor isolated from catfish leucocytes that has both bactericidal and antiviral activities. Oxygen-independent mechanisms of innate immunity for killing microorganisms have not been identified in leucocytes of channel catfish. Leucocytes were separated from catfish blood, and granule extracts were obtained by homogenization, centrifugation, and extraction with 10% acetic acid. The granule extracts were further purified by gel filtration chromatography. Bactericidal assays against the two fish pathogens and SDS–PAGE analysis were done on the isolated antimicrobial factor. Determination of antiviral activity of the factor was done by in vitro tissue culture using herpes simplex virus-type 1. Mass spectrometry analyses were done for molecular weight (655
Da), purity, and structural characterization of the innate non-peptide antimicrobial factor. |
| doi_str_mv | 10.1016/j.bbrc.2003.11.093 |
| format | Article |
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Ictalurus punctatus) is extensively used in aquaculture in the Southeast US and is susceptible to many bacterial infections acquired from its pond environment. Research is needed to better understand the defensive response and innate immunity of channel catfish against fish pathogens like
Edwardsiella ictaluri and
Aeromonas hydrophila. The main objectives were purification and characterization of an innate antimicrobial factor isolated from catfish leucocytes that has both bactericidal and antiviral activities. Oxygen-independent mechanisms of innate immunity for killing microorganisms have not been identified in leucocytes of channel catfish. Leucocytes were separated from catfish blood, and granule extracts were obtained by homogenization, centrifugation, and extraction with 10% acetic acid. The granule extracts were further purified by gel filtration chromatography. Bactericidal assays against the two fish pathogens and SDS–PAGE analysis were done on the isolated antimicrobial factor. Determination of antiviral activity of the factor was done by in vitro tissue culture using herpes simplex virus-type 1. Mass spectrometry analyses were done for molecular weight (655
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Ictalurus punctatus) is extensively used in aquaculture in the Southeast US and is susceptible to many bacterial infections acquired from its pond environment. Research is needed to better understand the defensive response and innate immunity of channel catfish against fish pathogens like
Edwardsiella ictaluri and
Aeromonas hydrophila. The main objectives were purification and characterization of an innate antimicrobial factor isolated from catfish leucocytes that has both bactericidal and antiviral activities. Oxygen-independent mechanisms of innate immunity for killing microorganisms have not been identified in leucocytes of channel catfish. Leucocytes were separated from catfish blood, and granule extracts were obtained by homogenization, centrifugation, and extraction with 10% acetic acid. The granule extracts were further purified by gel filtration chromatography. Bactericidal assays against the two fish pathogens and SDS–PAGE analysis were done on the isolated antimicrobial factor. Determination of antiviral activity of the factor was done by in vitro tissue culture using herpes simplex virus-type 1. Mass spectrometry analyses were done for molecular weight (655
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Chung, Kyung T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c383t-50059b3ee1d411c96e042673c7bacaa09b47316031641ae15ef75b76651723c23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Aeromonas hydrophila</topic><topic>Aeromonas hydrophila - drug effects</topic><topic>Animals</topic><topic>Anti-Bacterial Agents - blood</topic><topic>Anti-Bacterial Agents - chemistry</topic><topic>Anti-Bacterial Agents - isolation & purification</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antimicrobial factor</topic><topic>Antiviral Agents - chemistry</topic><topic>Antiviral Agents - isolation & purification</topic><topic>Antiviral Agents - pharmacology</topic><topic>Bactericidal</topic><topic>Blood Proteins - chemistry</topic><topic>Blood Proteins - isolation & purification</topic><topic>Blood Proteins - pharmacology</topic><topic>Catfishes - blood</topic><topic>Channel catfish</topic><topic>Cytoplasmic Granules - chemistry</topic><topic>Diffusion</topic><topic>Edwardsiella ictaluri</topic><topic>Edwardsiella ictaluri - drug effects</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Escherichia coli - drug effects</topic><topic>Freshwater</topic><topic>Herpesvirus 1, Human - drug effects</topic><topic>Ictalurus punctatus</topic><topic>Leukocytes - chemistry</topic><topic>Microbial Sensitivity Tests - methods</topic><topic>Molecular Weight</topic><topic>Purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ourth, Donald D</creatorcontrib><creatorcontrib>Chung, Kyung T</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ourth, Donald D</au><au>Chung, Kyung T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification of antimicrobial factor from granules of channel catfish peripheral blood leucocytes</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2004-01-02</date><risdate>2004</risdate><volume>313</volume><issue>1</issue><spage>28</spage><epage>36</epage><pages>28-36</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>The channel catfish (
Ictalurus punctatus) is extensively used in aquaculture in the Southeast US and is susceptible to many bacterial infections acquired from its pond environment. Research is needed to better understand the defensive response and innate immunity of channel catfish against fish pathogens like
Edwardsiella ictaluri and
Aeromonas hydrophila. The main objectives were purification and characterization of an innate antimicrobial factor isolated from catfish leucocytes that has both bactericidal and antiviral activities. Oxygen-independent mechanisms of innate immunity for killing microorganisms have not been identified in leucocytes of channel catfish. Leucocytes were separated from catfish blood, and granule extracts were obtained by homogenization, centrifugation, and extraction with 10% acetic acid. The granule extracts were further purified by gel filtration chromatography. Bactericidal assays against the two fish pathogens and SDS–PAGE analysis were done on the isolated antimicrobial factor. Determination of antiviral activity of the factor was done by in vitro tissue culture using herpes simplex virus-type 1. Mass spectrometry analyses were done for molecular weight (655
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| subjects | Aeromonas hydrophila Aeromonas hydrophila - drug effects Animals Anti-Bacterial Agents - blood Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - isolation & purification Anti-Bacterial Agents - pharmacology Antimicrobial factor Antiviral Agents - chemistry Antiviral Agents - isolation & purification Antiviral Agents - pharmacology Bactericidal Blood Proteins - chemistry Blood Proteins - isolation & purification Blood Proteins - pharmacology Catfishes - blood Channel catfish Cytoplasmic Granules - chemistry Diffusion Edwardsiella ictaluri Edwardsiella ictaluri - drug effects Electrophoresis, Polyacrylamide Gel Escherichia coli - drug effects Freshwater Herpesvirus 1, Human - drug effects Ictalurus punctatus Leukocytes - chemistry Microbial Sensitivity Tests - methods Molecular Weight Purification |
| title | Purification of antimicrobial factor from granules of channel catfish peripheral blood leucocytes |
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