Composition of surface oxide film of titanium with culturing murine fibroblasts L929
Changes in the composition of surface oxide film on titanium specimens in the presence of amino acids, serum proteins, and cells were characterized using X-ray photoelectron spectroscopy. The surface oxide film on titanium formed in the air is so protective that the further oxidation of titanium is...
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| Veröffentlicht in: | Biomaterials 2004-03, Vol.25 (6), p.979-986 |
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| creator | Hiromoto, Sachiko Hanawa, Takao Asami, Katsuhiko |
| description | Changes in the composition of surface oxide film on titanium specimens in the presence of amino acids, serum proteins, and cells were characterized using X-ray photoelectron spectroscopy. The surface oxide film on titanium formed in the air is so protective that the further oxidation of titanium is prevented in various circumstances. During immersion of the specimen in Hanks’ solution, Eagle’s minimum essential medium (MEM), and MEM with the addition of fetal bovine serum (MEM+FBS), calcium phosphate precipitated, causing the increase in thickness of the surface oxide film. Calcium phosphate was also precipitated with culturing murine fibroblast L929, but the amount of the calcium phosphate was smaller than those in Hanks’ solution, MEM, and MEM+FBS. The relative concentration ratio of calcium to phosphorous, [Ca]/[P], increased with proteins charging negatively, while the ratio decreased with the cells whose extracellular matrix charging positively. In addition, sulfur precipitated as S
0 and/or S
2− only with culturing the cells. Sulfate ions in the MEM+FBS are reduced at the interface between titanium and the solution with the existence of cells. |
| doi_str_mv | 10.1016/S0142-9612(03)00620-3 |
| format | Article |
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2− only with culturing the cells. Sulfate ions in the MEM+FBS are reduced at the interface between titanium and the solution with the existence of cells.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/S0142-9612(03)00620-3</identifier><identifier>PMID: 14615162</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Adsorption ; Amino acid ; Amino Acids - chemistry ; Animals ; Biomaterial ; Calcium Phosphates - chemistry ; Calcium Phosphates - metabolism ; Cell Adhesion - physiology ; Cell culture ; Cell Division - physiology ; Cell Line ; Coated Materials, Biocompatible - chemistry ; Fibroblasts - physiology ; Isotonic Solutions - chemistry ; Materials Testing ; Mice ; Protein ; Proteins - chemistry ; Serum Albumin, Bovine - chemistry ; Surface analysis ; Surface Properties ; Titanium ; Titanium - chemistry</subject><ispartof>Biomaterials, 2004-03, Vol.25 (6), p.979-986</ispartof><rights>2003 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c485t-e8444580e365095b5674c5cdbe8232a983d55cdb818e789ced12dca64d80440a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0142961203006203$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14615162$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hiromoto, Sachiko</creatorcontrib><creatorcontrib>Hanawa, Takao</creatorcontrib><creatorcontrib>Asami, Katsuhiko</creatorcontrib><title>Composition of surface oxide film of titanium with culturing murine fibroblasts L929</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Changes in the composition of surface oxide film on titanium specimens in the presence of amino acids, serum proteins, and cells were characterized using X-ray photoelectron spectroscopy. The surface oxide film on titanium formed in the air is so protective that the further oxidation of titanium is prevented in various circumstances. During immersion of the specimen in Hanks’ solution, Eagle’s minimum essential medium (MEM), and MEM with the addition of fetal bovine serum (MEM+FBS), calcium phosphate precipitated, causing the increase in thickness of the surface oxide film. Calcium phosphate was also precipitated with culturing murine fibroblast L929, but the amount of the calcium phosphate was smaller than those in Hanks’ solution, MEM, and MEM+FBS. The relative concentration ratio of calcium to phosphorous, [Ca]/[P], increased with proteins charging negatively, while the ratio decreased with the cells whose extracellular matrix charging positively. In addition, sulfur precipitated as S
0 and/or S
2− only with culturing the cells. Sulfate ions in the MEM+FBS are reduced at the interface between titanium and the solution with the existence of cells.</description><subject>Adsorption</subject><subject>Amino acid</subject><subject>Amino Acids - chemistry</subject><subject>Animals</subject><subject>Biomaterial</subject><subject>Calcium Phosphates - chemistry</subject><subject>Calcium Phosphates - metabolism</subject><subject>Cell Adhesion - physiology</subject><subject>Cell culture</subject><subject>Cell Division - physiology</subject><subject>Cell Line</subject><subject>Coated Materials, Biocompatible - chemistry</subject><subject>Fibroblasts - physiology</subject><subject>Isotonic Solutions - chemistry</subject><subject>Materials Testing</subject><subject>Mice</subject><subject>Protein</subject><subject>Proteins - chemistry</subject><subject>Serum Albumin, Bovine - chemistry</subject><subject>Surface analysis</subject><subject>Surface Properties</subject><subject>Titanium</subject><subject>Titanium - chemistry</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQQC0EotvCTwDlhOghdMaxE_uEqhUflVbi0HK2HGcCRkm82A7Qf0_SXcFxexrN6M2MZh5jrxDeIWB9dQsoeKlr5G-hugSoOZTVE7ZB1ahSapBP2eYfcsbOU_oBSw6CP2dnKGqUWPMNu9uGcR-Szz5MReiLNMfeOirCH99R0fthXKvZZzv5eSx--_y9cPOQ5-inb8W4hhVrY2gHm3IqdprrF-xZb4dEL4_xgn39-OFu-7ncffl0s73elU4omUtSQgipgKpagpatrBvhpOtaUrziVquqk2uqUFGjtKMOeedsLToFQoCtLtibw9x9DD9nStmMPjkaBjtRmJNpUMpGQnUS5AoRGoDHgCAFNI8AoVFC6pMgas65VriA8gC6GFKK1Jt99KON9wbBrMrNg3Kz-jRQmQflZr3t9XHB3I7U_e86Ol6A9weAFhW_PEWTnKdpeaeP5LLpgj-x4i8xCLlz</recordid><startdate>20040301</startdate><enddate>20040301</enddate><creator>Hiromoto, Sachiko</creator><creator>Hanawa, Takao</creator><creator>Asami, Katsuhiko</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SE</scope><scope>8BQ</scope><scope>JG9</scope><scope>F28</scope><scope>7QQ</scope><scope>7X8</scope></search><sort><creationdate>20040301</creationdate><title>Composition of surface oxide film of titanium with culturing murine fibroblasts L929</title><author>Hiromoto, Sachiko ; Hanawa, Takao ; Asami, Katsuhiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c485t-e8444580e365095b5674c5cdbe8232a983d55cdb818e789ced12dca64d80440a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adsorption</topic><topic>Amino acid</topic><topic>Amino Acids - chemistry</topic><topic>Animals</topic><topic>Biomaterial</topic><topic>Calcium Phosphates - chemistry</topic><topic>Calcium Phosphates - metabolism</topic><topic>Cell Adhesion - physiology</topic><topic>Cell culture</topic><topic>Cell Division - physiology</topic><topic>Cell Line</topic><topic>Coated Materials, Biocompatible - chemistry</topic><topic>Fibroblasts - physiology</topic><topic>Isotonic Solutions - chemistry</topic><topic>Materials Testing</topic><topic>Mice</topic><topic>Protein</topic><topic>Proteins - chemistry</topic><topic>Serum Albumin, Bovine - chemistry</topic><topic>Surface analysis</topic><topic>Surface Properties</topic><topic>Titanium</topic><topic>Titanium - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hiromoto, Sachiko</creatorcontrib><creatorcontrib>Hanawa, Takao</creatorcontrib><creatorcontrib>Asami, Katsuhiko</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Corrosion Abstracts</collection><collection>METADEX</collection><collection>Materials Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Ceramic Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hiromoto, Sachiko</au><au>Hanawa, Takao</au><au>Asami, Katsuhiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Composition of surface oxide film of titanium with culturing murine fibroblasts L929</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2004-03-01</date><risdate>2004</risdate><volume>25</volume><issue>6</issue><spage>979</spage><epage>986</epage><pages>979-986</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>Changes in the composition of surface oxide film on titanium specimens in the presence of amino acids, serum proteins, and cells were characterized using X-ray photoelectron spectroscopy. The surface oxide film on titanium formed in the air is so protective that the further oxidation of titanium is prevented in various circumstances. During immersion of the specimen in Hanks’ solution, Eagle’s minimum essential medium (MEM), and MEM with the addition of fetal bovine serum (MEM+FBS), calcium phosphate precipitated, causing the increase in thickness of the surface oxide film. Calcium phosphate was also precipitated with culturing murine fibroblast L929, but the amount of the calcium phosphate was smaller than those in Hanks’ solution, MEM, and MEM+FBS. The relative concentration ratio of calcium to phosphorous, [Ca]/[P], increased with proteins charging negatively, while the ratio decreased with the cells whose extracellular matrix charging positively. In addition, sulfur precipitated as S
0 and/or S
2− only with culturing the cells. Sulfate ions in the MEM+FBS are reduced at the interface between titanium and the solution with the existence of cells.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>14615162</pmid><doi>10.1016/S0142-9612(03)00620-3</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Adsorption Amino acid Amino Acids - chemistry Animals Biomaterial Calcium Phosphates - chemistry Calcium Phosphates - metabolism Cell Adhesion - physiology Cell culture Cell Division - physiology Cell Line Coated Materials, Biocompatible - chemistry Fibroblasts - physiology Isotonic Solutions - chemistry Materials Testing Mice Protein Proteins - chemistry Serum Albumin, Bovine - chemistry Surface analysis Surface Properties Titanium Titanium - chemistry |
| title | Composition of surface oxide film of titanium with culturing murine fibroblasts L929 |
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