A microfabricated array bioreactor for perfused 3D liver culture

We describe the design, fabrication, and performance of a bioreactor that enables both morphogenesis of 3D tissue structures under continuous perfusion and repeated in situ observation by light microscopy. Three‐dimensional scaffolds were created by deep reactive ion etching of silicon wafers to cre...

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Veröffentlicht in:	Biotechnology and bioengineering 2002-05, Vol.78 (3), p.257-269
Hauptverfasser:	Powers, Mark J., Domansky, Karel, Kaazempur-Mofrad, Mohammad R., Kalezi, Artemis, Capitano, Adam, Upadhyaya, Arpita, Kurzawski, Petra, Wack, Kathryn E., Stolz, Donna Beer, Kamm, Roger, Griffith, Linda G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences bioreactor Bioreactors Biotechnology Cell Culture Techniques Fundamental and applied biological sciences. Psychology gene delivery Green Fluorescent Proteins Hepatocytes - cytology Hepatocytes - physiology Imaging, Three-Dimensional liver Luminescent Proteins - genetics Luminescent Proteins - metabolism Male Methods. Procedures. Technologies Microscopy Morphogenesis - physiology Organ Culture Techniques - methods Organ Culture Techniques - trends Oxygen - physiology Perfusion Rats Space life sciences Stress, Mechanical tissue engineering Tissue Engineering - methods Tissue Engineering - trends Various methods and equipments
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