Messenger Ribonucleic Acid Expressions of Hepatocyte Growth Factor, Angiopoietins and Their Receptors During Follicular Development in Gilts
Angiogenic factors are associated with angiogenesis during follicular development in the mammalian ovary. The aim of the present study was to determine the relationships between the vascular network and mRNA expressions of angiopoietins (Ang)-1, Ang-2 and hepatocyte growth factor (HGF), and their re...
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| Veröffentlicht in: | Journal of Reproduction and Development 2003, Vol.49(3), pp.203-211 |
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| creator | SHIMIZU, Takashi IIJIMA, Koji SASADA, Hiroshi SATO, Eimei |
| description | Angiogenic factors are associated with angiogenesis during follicular development in the mammalian ovary. The aim of the present study was to determine the relationships between the vascular network and mRNA expressions of angiopoietins (Ang)-1, Ang-2 and hepatocyte growth factor (HGF), and their receptors in follicles at different developmental stages during follicular development. Ovaries in gilts were collected 72 h after equine chorionic gonadotropin (eCG, 1250 IU) treatment for histological observation of the capillary network. Granulosa cells and thecal tissues in small (5 mm) individual follicles were collected for detection of mRNA expression of HGF, Ang-1 and Ang-2 in granulosa cells, and HGF receptor (HGF-R) and Tie-2 in the theca cells by semi-quantitative RT-PCR. The number of capillaries in the thecal cell layer increased significantly in healthy follicles at all developmental stages in the eCG group compared with those in controls. The expression of Ang-1 mRNA declined in granulosa cells of medium and large follicles and the level of Ang-2 mRNA increased in granulosa cells of small follicles after eCG treatment. The ratio of Ang-2/Ang-1 increased in small, medium and large follicles from ovaries after eCG treatment, but Tie-2 mRNA expression in the theca cells did not change. The level of HGF mRNA increased in granulosa cells of small follicles after eCG treatment but HGF-R in theca cells was not increased by eCG. These data suggested that the angiopoietins might be associated with thecal angiogenesis during follicular development in eCG-treated gilts. |
| doi_str_mv | 10.1262/jrd.49.203 |
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The aim of the present study was to determine the relationships between the vascular network and mRNA expressions of angiopoietins (Ang)-1, Ang-2 and hepatocyte growth factor (HGF), and their receptors in follicles at different developmental stages during follicular development. Ovaries in gilts were collected 72 h after equine chorionic gonadotropin (eCG, 1250 IU) treatment for histological observation of the capillary network. Granulosa cells and thecal tissues in small (<4 mm), medium (4-5 mm) or large (>5 mm) individual follicles were collected for detection of mRNA expression of HGF, Ang-1 and Ang-2 in granulosa cells, and HGF receptor (HGF-R) and Tie-2 in the theca cells by semi-quantitative RT-PCR. The number of capillaries in the thecal cell layer increased significantly in healthy follicles at all developmental stages in the eCG group compared with those in controls. The expression of Ang-1 mRNA declined in granulosa cells of medium and large follicles and the level of Ang-2 mRNA increased in granulosa cells of small follicles after eCG treatment. The ratio of Ang-2/Ang-1 increased in small, medium and large follicles from ovaries after eCG treatment, but Tie-2 mRNA expression in the theca cells did not change. The level of HGF mRNA increased in granulosa cells of small follicles after eCG treatment but HGF-R in theca cells was not increased by eCG. These data suggested that the angiopoietins might be associated with thecal angiogenesis during follicular development in eCG-treated gilts.</description><identifier>ISSN: 0916-8818</identifier><identifier>EISSN: 1348-4400</identifier><identifier>DOI: 10.1262/jrd.49.203</identifier><identifier>PMID: 14967929</identifier><language>eng</language><publisher>Japan: THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT</publisher><subject>Angiogenesis ; Angiopoietin-1 - genetics ; Angiopoietin-2 - genetics ; angiopoietins ; Animals ; Base Sequence ; Capillaries - drug effects ; Capillaries - growth & development ; Chorionic Gonadotropin - pharmacology ; DNA Primers - genetics ; Female ; Follicular Development ; Granulosa Cells - drug effects ; Granulosa Cells - metabolism ; Hepatocyte Growth Factor - genetics ; HGF ; Ovarian Follicle - blood supply ; Ovarian Follicle - drug effects ; Ovarian Follicle - physiology ; Pig ; Proto-Oncogene Proteins c-met - genetics ; Receptor, TIE-2 - genetics ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Swine ; Theca Cells - drug effects ; Theca Cells - metabolism</subject><ispartof>Journal of Reproduction and Development, 2003, Vol.49(3), pp.203-211</ispartof><rights>2003 Society for Reproduction and Development</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c511t-6d68437c96f1ab9282431fdef6c8f8297b1483e8af5102b1cc16dcf4478c97b03</citedby><cites>FETCH-LOGICAL-c511t-6d68437c96f1ab9282431fdef6c8f8297b1483e8af5102b1cc16dcf4478c97b03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,1883,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14967929$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SHIMIZU, Takashi</creatorcontrib><creatorcontrib>IIJIMA, Koji</creatorcontrib><creatorcontrib>SASADA, Hiroshi</creatorcontrib><creatorcontrib>SATO, Eimei</creatorcontrib><title>Messenger Ribonucleic Acid Expressions of Hepatocyte Growth Factor, Angiopoietins and Their Receptors During Follicular Development in Gilts</title><title>Journal of Reproduction and Development</title><addtitle>J. Reprod. Dev.</addtitle><description>Angiogenic factors are associated with angiogenesis during follicular development in the mammalian ovary. The aim of the present study was to determine the relationships between the vascular network and mRNA expressions of angiopoietins (Ang)-1, Ang-2 and hepatocyte growth factor (HGF), and their receptors in follicles at different developmental stages during follicular development. Ovaries in gilts were collected 72 h after equine chorionic gonadotropin (eCG, 1250 IU) treatment for histological observation of the capillary network. Granulosa cells and thecal tissues in small (<4 mm), medium (4-5 mm) or large (>5 mm) individual follicles were collected for detection of mRNA expression of HGF, Ang-1 and Ang-2 in granulosa cells, and HGF receptor (HGF-R) and Tie-2 in the theca cells by semi-quantitative RT-PCR. The number of capillaries in the thecal cell layer increased significantly in healthy follicles at all developmental stages in the eCG group compared with those in controls. The expression of Ang-1 mRNA declined in granulosa cells of medium and large follicles and the level of Ang-2 mRNA increased in granulosa cells of small follicles after eCG treatment. The ratio of Ang-2/Ang-1 increased in small, medium and large follicles from ovaries after eCG treatment, but Tie-2 mRNA expression in the theca cells did not change. The level of HGF mRNA increased in granulosa cells of small follicles after eCG treatment but HGF-R in theca cells was not increased by eCG. These data suggested that the angiopoietins might be associated with thecal angiogenesis during follicular development in eCG-treated gilts.</description><subject>Angiogenesis</subject><subject>Angiopoietin-1 - genetics</subject><subject>Angiopoietin-2 - genetics</subject><subject>angiopoietins</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Capillaries - drug effects</subject><subject>Capillaries - growth & development</subject><subject>Chorionic Gonadotropin - pharmacology</subject><subject>DNA Primers - genetics</subject><subject>Female</subject><subject>Follicular Development</subject><subject>Granulosa Cells - drug effects</subject><subject>Granulosa Cells - metabolism</subject><subject>Hepatocyte Growth Factor - genetics</subject><subject>HGF</subject><subject>Ovarian Follicle - blood supply</subject><subject>Ovarian Follicle - drug effects</subject><subject>Ovarian Follicle - physiology</subject><subject>Pig</subject><subject>Proto-Oncogene Proteins c-met - genetics</subject><subject>Receptor, TIE-2 - genetics</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Swine</subject><subject>Theca Cells - drug effects</subject><subject>Theca Cells - metabolism</subject><issn>0916-8818</issn><issn>1348-4400</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkM1uEzEURi0EomlgwwMgr1igTuq_zNgrFLVNilRUCZW15fHcSRxN7MH2FPoOPDSuEsrm3sV3dBYHoQ-ULCir2eU-dguhFozwV2hGuZCVEIS8RjOiaF1JSeUZOk9pTwhny1q8RWdUqLpRTM3Qn2-QEvgtRPzdtcFPdgBn8cq6Dt_8HmNZXfAJhx7fwmhysE8Z8CaGX3mH18bmEC_wym9dGIOD7ApqfIcfduCKESyMhUj4eorOb_E6DIOz02AivoZHGMJ4AJ-x83jjhpzeoTe9GRK8P_05-rG-ebi6re7uN1-vVneVXVKaq7qrpeCNVXVPTauYZILTvoO-trKXTDUtFZKDNP2SEtZSa2nd2V6IRtoyEj5Hn47eMYafE6SsDy5ZGAbjIUxJN3Qp5LN1jj4fQRtDShF6PUZ3MPFJU6Kf2-vSXgulS_sCfzxZp_YA3X_0FLsAX47APmWzhRfAxOxK9n8ufjxF-bLYnYkaPP8L1o6ZCg</recordid><startdate>2003</startdate><enddate>2003</enddate><creator>SHIMIZU, Takashi</creator><creator>IIJIMA, Koji</creator><creator>SASADA, Hiroshi</creator><creator>SATO, Eimei</creator><general>THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2003</creationdate><title>Messenger Ribonucleic Acid Expressions of Hepatocyte Growth Factor, Angiopoietins and Their Receptors During Follicular Development in Gilts</title><author>SHIMIZU, Takashi ; IIJIMA, Koji ; SASADA, Hiroshi ; SATO, Eimei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c511t-6d68437c96f1ab9282431fdef6c8f8297b1483e8af5102b1cc16dcf4478c97b03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Angiogenesis</topic><topic>Angiopoietin-1 - genetics</topic><topic>Angiopoietin-2 - genetics</topic><topic>angiopoietins</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Capillaries - drug effects</topic><topic>Capillaries - growth & development</topic><topic>Chorionic Gonadotropin - pharmacology</topic><topic>DNA Primers - genetics</topic><topic>Female</topic><topic>Follicular Development</topic><topic>Granulosa Cells - drug effects</topic><topic>Granulosa Cells - metabolism</topic><topic>Hepatocyte Growth Factor - genetics</topic><topic>HGF</topic><topic>Ovarian Follicle - blood supply</topic><topic>Ovarian Follicle - drug effects</topic><topic>Ovarian Follicle - physiology</topic><topic>Pig</topic><topic>Proto-Oncogene Proteins c-met - genetics</topic><topic>Receptor, TIE-2 - genetics</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Swine</topic><topic>Theca Cells - drug effects</topic><topic>Theca Cells - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SHIMIZU, Takashi</creatorcontrib><creatorcontrib>IIJIMA, Koji</creatorcontrib><creatorcontrib>SASADA, Hiroshi</creatorcontrib><creatorcontrib>SATO, Eimei</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Reproduction and Development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SHIMIZU, Takashi</au><au>IIJIMA, Koji</au><au>SASADA, Hiroshi</au><au>SATO, Eimei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Messenger Ribonucleic Acid Expressions of Hepatocyte Growth Factor, Angiopoietins and Their Receptors During Follicular Development in Gilts</atitle><jtitle>Journal of Reproduction and Development</jtitle><addtitle>J. Reprod. Dev.</addtitle><date>2003</date><risdate>2003</risdate><volume>49</volume><issue>3</issue><spage>203</spage><epage>211</epage><pages>203-211</pages><issn>0916-8818</issn><eissn>1348-4400</eissn><abstract>Angiogenic factors are associated with angiogenesis during follicular development in the mammalian ovary. The aim of the present study was to determine the relationships between the vascular network and mRNA expressions of angiopoietins (Ang)-1, Ang-2 and hepatocyte growth factor (HGF), and their receptors in follicles at different developmental stages during follicular development. Ovaries in gilts were collected 72 h after equine chorionic gonadotropin (eCG, 1250 IU) treatment for histological observation of the capillary network. Granulosa cells and thecal tissues in small (<4 mm), medium (4-5 mm) or large (>5 mm) individual follicles were collected for detection of mRNA expression of HGF, Ang-1 and Ang-2 in granulosa cells, and HGF receptor (HGF-R) and Tie-2 in the theca cells by semi-quantitative RT-PCR. The number of capillaries in the thecal cell layer increased significantly in healthy follicles at all developmental stages in the eCG group compared with those in controls. The expression of Ang-1 mRNA declined in granulosa cells of medium and large follicles and the level of Ang-2 mRNA increased in granulosa cells of small follicles after eCG treatment. The ratio of Ang-2/Ang-1 increased in small, medium and large follicles from ovaries after eCG treatment, but Tie-2 mRNA expression in the theca cells did not change. The level of HGF mRNA increased in granulosa cells of small follicles after eCG treatment but HGF-R in theca cells was not increased by eCG. These data suggested that the angiopoietins might be associated with thecal angiogenesis during follicular development in eCG-treated gilts.</abstract><cop>Japan</cop><pub>THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT</pub><pmid>14967929</pmid><doi>10.1262/jrd.49.203</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | J-STAGE Free; MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; AgriKnowledge（アグリナレッジ）AGROLib |
| subjects | Angiogenesis Angiopoietin-1 - genetics Angiopoietin-2 - genetics angiopoietins Animals Base Sequence Capillaries - drug effects Capillaries - growth & development Chorionic Gonadotropin - pharmacology DNA Primers - genetics Female Follicular Development Granulosa Cells - drug effects Granulosa Cells - metabolism Hepatocyte Growth Factor - genetics HGF Ovarian Follicle - blood supply Ovarian Follicle - drug effects Ovarian Follicle - physiology Pig Proto-Oncogene Proteins c-met - genetics Receptor, TIE-2 - genetics RNA, Messenger - genetics RNA, Messenger - metabolism Swine Theca Cells - drug effects Theca Cells - metabolism |
| title | Messenger Ribonucleic Acid Expressions of Hepatocyte Growth Factor, Angiopoietins and Their Receptors During Follicular Development in Gilts |
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