Monitoring of idebenone treatment in patients with Friedreich's ataxia by high-pressure liquid chromatography with electrochemical detection
Idebenone is a quinone analog that is applied in the treatment of several neurological disorders including Friedreich ataxia and mitochondrial encephalomyopathies. Our aim was to develop an easy and sensitive analytical HPLC-procedure for the determination of idebenone in the serum of patients treat...
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| Veröffentlicht in: | Journal of neuroscience methods 2002-03, Vol.115 (1), p.63-66 |
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| creator | Artuch, Rafael Colomé, Catrina Vilaseca, Maria Antònia Aracil, Asun Pineda, Mercè |
| description | Idebenone is a quinone analog that is applied in the treatment of several neurological disorders including Friedreich ataxia and mitochondrial encephalomyopathies. Our aim was to develop an easy and sensitive analytical HPLC-procedure for the determination of idebenone in the serum of patients treated with this drug. Serum samples from nine paediatric patients diagnosed with Friedreich ataxia and receiving idebenone treatment were analyzed. Idebenone was separated from serum by reverse high-pressure liquid chromatography and analyzed using an electrochemical detection procedure. No interferences were observed during analysis of patient samples obtained prior to idebenone treatment. Calibration of idebenone concentration indicated a linear range between 500 pmol/l and 5 μmol/l and calculation of within-run and between-run coefficients of variation suggested adequate analytical quality for reliable determination. In agreement with previously reported data, during drug therapy, idebenone serum concentrations (basal conditions, range 0.1−0.49 μmol/l) were greatly elevated 90 min after an oral dose (range 0.66–3.63 μmol/l). Thus, we have developed a simple and rapid method that offers adequate analytical quality for accurate idebenone determination. |
| doi_str_mv | 10.1016/S0165-0270(01)00533-7 |
| format | Article |
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Our aim was to develop an easy and sensitive analytical HPLC-procedure for the determination of idebenone in the serum of patients treated with this drug. Serum samples from nine paediatric patients diagnosed with Friedreich ataxia and receiving idebenone treatment were analyzed. Idebenone was separated from serum by reverse high-pressure liquid chromatography and analyzed using an electrochemical detection procedure. No interferences were observed during analysis of patient samples obtained prior to idebenone treatment. Calibration of idebenone concentration indicated a linear range between 500 pmol/l and 5 μmol/l and calculation of within-run and between-run coefficients of variation suggested adequate analytical quality for reliable determination. In agreement with previously reported data, during drug therapy, idebenone serum concentrations (basal conditions, range 0.1−0.49 μmol/l) were greatly elevated 90 min after an oral dose (range 0.66–3.63 μmol/l). Thus, we have developed a simple and rapid method that offers adequate analytical quality for accurate idebenone determination.</description><identifier>ISSN: 0165-0270</identifier><identifier>EISSN: 1872-678X</identifier><identifier>DOI: 10.1016/S0165-0270(01)00533-7</identifier><identifier>PMID: 11897364</identifier><identifier>CODEN: JNMEDT</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Adolescent ; Antioxidants - pharmacokinetics ; Benzoquinones - blood ; Benzoquinones - pharmacokinetics ; Biological and medical sciences ; Child ; Chromatography, High Pressure Liquid - instrumentation ; Chromatography, High Pressure Liquid - methods ; Electrochemical detection ; Electrochemistry - instrumentation ; Electrochemistry - methods ; Female ; Friedreich ataxia ; Friedreich Ataxia - drug therapy ; Fundamental and applied biological sciences. Psychology ; General aspects. Models. Methods ; HPLC ; Humans ; Idebenone ; Male ; Monitoring, Physiologic - instrumentation ; Monitoring, Physiologic - methods ; Pediatric patients ; Pharmacology, Clinical - instrumentation ; Pharmacology, Clinical - methods ; Ubiquinone ; Ubiquinone - analogs & derivatives ; Vertebrates: nervous system and sense organs</subject><ispartof>Journal of neuroscience methods, 2002-03, Vol.115 (1), p.63-66</ispartof><rights>2002 Elsevier Science B.V.</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-6f79ec4c23d2d5b87036a295976f8db22bb79f8f333032b220356182c682fba33</citedby><cites>FETCH-LOGICAL-c417t-6f79ec4c23d2d5b87036a295976f8db22bb79f8f333032b220356182c682fba33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0165027001005337$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13538894$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11897364$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Artuch, Rafael</creatorcontrib><creatorcontrib>Colomé, Catrina</creatorcontrib><creatorcontrib>Vilaseca, Maria Antònia</creatorcontrib><creatorcontrib>Aracil, Asun</creatorcontrib><creatorcontrib>Pineda, Mercè</creatorcontrib><title>Monitoring of idebenone treatment in patients with Friedreich's ataxia by high-pressure liquid chromatography with electrochemical detection</title><title>Journal of neuroscience methods</title><addtitle>J Neurosci Methods</addtitle><description>Idebenone is a quinone analog that is applied in the treatment of several neurological disorders including Friedreich ataxia and mitochondrial encephalomyopathies. Our aim was to develop an easy and sensitive analytical HPLC-procedure for the determination of idebenone in the serum of patients treated with this drug. Serum samples from nine paediatric patients diagnosed with Friedreich ataxia and receiving idebenone treatment were analyzed. Idebenone was separated from serum by reverse high-pressure liquid chromatography and analyzed using an electrochemical detection procedure. No interferences were observed during analysis of patient samples obtained prior to idebenone treatment. Calibration of idebenone concentration indicated a linear range between 500 pmol/l and 5 μmol/l and calculation of within-run and between-run coefficients of variation suggested adequate analytical quality for reliable determination. In agreement with previously reported data, during drug therapy, idebenone serum concentrations (basal conditions, range 0.1−0.49 μmol/l) were greatly elevated 90 min after an oral dose (range 0.66–3.63 μmol/l). Thus, we have developed a simple and rapid method that offers adequate analytical quality for accurate idebenone determination.</description><subject>Adolescent</subject><subject>Antioxidants - pharmacokinetics</subject><subject>Benzoquinones - blood</subject><subject>Benzoquinones - pharmacokinetics</subject><subject>Biological and medical sciences</subject><subject>Child</subject><subject>Chromatography, High Pressure Liquid - instrumentation</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Electrochemical detection</subject><subject>Electrochemistry - instrumentation</subject><subject>Electrochemistry - methods</subject><subject>Female</subject><subject>Friedreich ataxia</subject><subject>Friedreich Ataxia - drug therapy</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects. Models. Methods</subject><subject>HPLC</subject><subject>Humans</subject><subject>Idebenone</subject><subject>Male</subject><subject>Monitoring, Physiologic - instrumentation</subject><subject>Monitoring, Physiologic - methods</subject><subject>Pediatric patients</subject><subject>Pharmacology, Clinical - instrumentation</subject><subject>Pharmacology, Clinical - methods</subject><subject>Ubiquinone</subject><subject>Ubiquinone - analogs & derivatives</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0165-0270</issn><issn>1872-678X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctu1DAUhi0EokPhEUDecFsEfEliZ4VQRQGpiAUgsbMc-2RyUBJPbQeYd-Ch8XRGdMnGN32_bX0_IY85e8UZb19_KUNTMaHYC8ZfMtZIWak7ZMO1ElWr9Pe7ZPMPOSMPUvrBGKs71t4nZ5zrTsm23pA_n8KCOURctjQMFD30sIQFaI5g8wxLprjQnc1Ylon-wjzSy4jgI6Abnydqs_2NlvZ7OuJ2rHYRUloj0AmvV_TUjTHMNodttLtxf8zDBC7H4EaY0dmJesjlAMPykNwb7JTg0Wk-J98u3329-FBdfX7_8eLtVeVqrnLVDqoDVzshvfBNrxWTrRVd06l20L4Xou9VN-hBSsmkKHsmm5Zr4Votht5KeU6eHe_dxXC9QspmxuRgmuwCYU1G8aZWtewK2BxBF0NKEQazizjbuDecmUMN5qYGc3BsGDc3NRhVck9OD6z9DP42dfJegKcnwKaiYIh2cZhuOdlIrbsD9-bIQdHxEyGa5EoTDjzG4sz4gP_5yl-yfKcx</recordid><startdate>20020330</startdate><enddate>20020330</enddate><creator>Artuch, Rafael</creator><creator>Colomé, Catrina</creator><creator>Vilaseca, Maria Antònia</creator><creator>Aracil, Asun</creator><creator>Pineda, Mercè</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020330</creationdate><title>Monitoring of idebenone treatment in patients with Friedreich's ataxia by high-pressure liquid chromatography with electrochemical detection</title><author>Artuch, Rafael ; Colomé, Catrina ; Vilaseca, Maria Antònia ; Aracil, Asun ; Pineda, Mercè</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-6f79ec4c23d2d5b87036a295976f8db22bb79f8f333032b220356182c682fba33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adolescent</topic><topic>Antioxidants - pharmacokinetics</topic><topic>Benzoquinones - blood</topic><topic>Benzoquinones - pharmacokinetics</topic><topic>Biological and medical sciences</topic><topic>Child</topic><topic>Chromatography, High Pressure Liquid - instrumentation</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Electrochemical detection</topic><topic>Electrochemistry - instrumentation</topic><topic>Electrochemistry - methods</topic><topic>Female</topic><topic>Friedreich ataxia</topic><topic>Friedreich Ataxia - drug therapy</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects. Models. Methods</topic><topic>HPLC</topic><topic>Humans</topic><topic>Idebenone</topic><topic>Male</topic><topic>Monitoring, Physiologic - instrumentation</topic><topic>Monitoring, Physiologic - methods</topic><topic>Pediatric patients</topic><topic>Pharmacology, Clinical - instrumentation</topic><topic>Pharmacology, Clinical - methods</topic><topic>Ubiquinone</topic><topic>Ubiquinone - analogs & derivatives</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Artuch, Rafael</creatorcontrib><creatorcontrib>Colomé, Catrina</creatorcontrib><creatorcontrib>Vilaseca, Maria Antònia</creatorcontrib><creatorcontrib>Aracil, Asun</creatorcontrib><creatorcontrib>Pineda, Mercè</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuroscience methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Artuch, Rafael</au><au>Colomé, Catrina</au><au>Vilaseca, Maria Antònia</au><au>Aracil, Asun</au><au>Pineda, Mercè</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Monitoring of idebenone treatment in patients with Friedreich's ataxia by high-pressure liquid chromatography with electrochemical detection</atitle><jtitle>Journal of neuroscience methods</jtitle><addtitle>J Neurosci Methods</addtitle><date>2002-03-30</date><risdate>2002</risdate><volume>115</volume><issue>1</issue><spage>63</spage><epage>66</epage><pages>63-66</pages><issn>0165-0270</issn><eissn>1872-678X</eissn><coden>JNMEDT</coden><abstract>Idebenone is a quinone analog that is applied in the treatment of several neurological disorders including Friedreich ataxia and mitochondrial encephalomyopathies. Our aim was to develop an easy and sensitive analytical HPLC-procedure for the determination of idebenone in the serum of patients treated with this drug. Serum samples from nine paediatric patients diagnosed with Friedreich ataxia and receiving idebenone treatment were analyzed. Idebenone was separated from serum by reverse high-pressure liquid chromatography and analyzed using an electrochemical detection procedure. No interferences were observed during analysis of patient samples obtained prior to idebenone treatment. Calibration of idebenone concentration indicated a linear range between 500 pmol/l and 5 μmol/l and calculation of within-run and between-run coefficients of variation suggested adequate analytical quality for reliable determination. In agreement with previously reported data, during drug therapy, idebenone serum concentrations (basal conditions, range 0.1−0.49 μmol/l) were greatly elevated 90 min after an oral dose (range 0.66–3.63 μmol/l). Thus, we have developed a simple and rapid method that offers adequate analytical quality for accurate idebenone determination.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>11897364</pmid><doi>10.1016/S0165-0270(01)00533-7</doi><tpages>4</tpages></addata></record> |
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| subjects | Adolescent Antioxidants - pharmacokinetics Benzoquinones - blood Benzoquinones - pharmacokinetics Biological and medical sciences Child Chromatography, High Pressure Liquid - instrumentation Chromatography, High Pressure Liquid - methods Electrochemical detection Electrochemistry - instrumentation Electrochemistry - methods Female Friedreich ataxia Friedreich Ataxia - drug therapy Fundamental and applied biological sciences. Psychology General aspects. Models. Methods HPLC Humans Idebenone Male Monitoring, Physiologic - instrumentation Monitoring, Physiologic - methods Pediatric patients Pharmacology, Clinical - instrumentation Pharmacology, Clinical - methods Ubiquinone Ubiquinone - analogs & derivatives Vertebrates: nervous system and sense organs |
| title | Monitoring of idebenone treatment in patients with Friedreich's ataxia by high-pressure liquid chromatography with electrochemical detection |
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