Quantitation of the mRNA levels of Epo and EpoR in various tissues in the ovine fetus

A partial cDNA of the sheep erythropoietin receptor (EpoR) was obtained and used in real-time PCR to quantitate mRNA levels in placenta, liver and kidney throughout development (term=150 days). This was compared with Epo mRNA levels in the same tissues. Both Epo and EpoR mRNA were present in the pla...

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Veröffentlicht in:	Molecular and cellular endocrinology 2002-02, Vol.188 (1), p.207-218
Hauptverfasser:	David, R.Bruce, Lim, Gaik Bee, Moritz, Karen M, Koukoulas, Irene, Wintour, E.Marelyn
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Base Sequence Cloning, Molecular Erythropoietin Erythropoietin - genetics Erythropoietin - metabolism Erythropoietin receptor Female Fetal Fetus - metabolism Gene Expression - drug effects Hippocampus - metabolism Immunoenzyme Techniques Kidney - embryology Kidney - metabolism Liver - embryology Liver - metabolism Molecular Sequence Data Organ Size Ovine Placenta Placenta - embryology Placenta - metabolism Pregnancy Real-time PCR Receptors, Erythropoietin - genetics Receptors, Erythropoietin - metabolism RNA, Messenger - metabolism Sequence Homology, Amino Acid Sheep - embryology
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