Structure and dynamics of the fusion pores in live GH-secreting cells revealed using atomic force microscopy
Earlier studies in live pancreatic acinar cells identified new cellular structures at the cell plasma membrane called 'pits' and 'depressions', where membrane-bound secretory vesicles dock and fuse to release vesicular contents. In the current study, using atomic force microscopy...
Gespeichert in:
| Veröffentlicht in: | Endocrinology (Philadelphia) 2002-03, Vol.143 (3), p.1144-1148 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1148 |
|---|---|
| container_issue | 3 |
| container_start_page | 1144 |
| container_title | Endocrinology (Philadelphia) |
| container_volume | 143 |
| creator | Cho, Sang-Joon Jeftinija, Ksenija Glavaski, Aleksandra Jeftinija, Srdija Jena, Bhanu P Anderson, Lloyd L |
| description | Earlier studies in live pancreatic acinar cells identified new cellular structures at the cell plasma membrane called 'pits' and 'depressions', where membrane-bound secretory vesicles dock and fuse to release vesicular contents. In the current study, using atomic force microscopy we identify similar structures at the plasma membrane of GH-secreting cells of the pituitary and implicate their involvement in hormone release. Pits containing 100-200 nm in diameter depressions or fusion pores were identified in resting GH-secreting cells. Following stimulation of secretion the size of depression enlarged and gold-tagged GH antibody were found to bind to the pit structures in the stimulated GH cells. This study documents for the first time the presence of these structures and their involvement in secretions in a neuroendocrine cell. |
| doi_str_mv | 10.1210/en.143.3.1144 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_71528735</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>71528735</sourcerecordid><originalsourceid>FETCH-LOGICAL-c290t-542b3233d888a0382e14c92d2016cffb82cfac0c836c02c423906c69c3f332a3</originalsourceid><addsrcrecordid>eNo1kDFPwzAQhT2AaCmMrMgTW4LtcxNnRBW0SJUY6B45lzMEJXGwk0r99wRRpnf39Onp6TF2J0UqlRSP1KdSQwqplFpfsKUQEpJcqXzBrmP8ml-tNVyxhZQmk2utlqx9H8OE4xSI277m9am3XYORe8fHT-Juio3v-eADRd70vG2OxLe7JBIGGpv-gyO1beSBjmRbqvnMz6Yd_ZzCnQ9IfL6Cj-iH0w27dLaNdHvWFTu8PB82u2T_tn3dPO0TVIUYk7lYBQqgNsZYAUaR1FioWgmZoXOVUegsCjSQoVCoFRQiw6xAcADKwoo9_MUOwX9PFMeya-JvT9uTn2KZy7UyOaxn8P4MTlVHdTmEprPhVP7PAz_1u2We</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71528735</pqid></control><display><type>article</type><title>Structure and dynamics of the fusion pores in live GH-secreting cells revealed using atomic force microscopy</title><source>MEDLINE</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Cho, Sang-Joon ; Jeftinija, Ksenija ; Glavaski, Aleksandra ; Jeftinija, Srdija ; Jena, Bhanu P ; Anderson, Lloyd L</creator><creatorcontrib>Cho, Sang-Joon ; Jeftinija, Ksenija ; Glavaski, Aleksandra ; Jeftinija, Srdija ; Jena, Bhanu P ; Anderson, Lloyd L</creatorcontrib><description>Earlier studies in live pancreatic acinar cells identified new cellular structures at the cell plasma membrane called 'pits' and 'depressions', where membrane-bound secretory vesicles dock and fuse to release vesicular contents. In the current study, using atomic force microscopy we identify similar structures at the plasma membrane of GH-secreting cells of the pituitary and implicate their involvement in hormone release. Pits containing 100-200 nm in diameter depressions or fusion pores were identified in resting GH-secreting cells. Following stimulation of secretion the size of depression enlarged and gold-tagged GH antibody were found to bind to the pit structures in the stimulated GH cells. This study documents for the first time the presence of these structures and their involvement in secretions in a neuroendocrine cell.</description><identifier>ISSN: 0013-7227</identifier><identifier>DOI: 10.1210/en.143.3.1144</identifier><identifier>PMID: 11861542</identifier><language>eng</language><publisher>United States</publisher><subject>Actins - metabolism ; Animals ; Cell Separation ; Growth Hormone - metabolism ; Hemolytic Plaque Technique ; Immunohistochemistry ; Microscopy, Atomic Force ; Neurosecretory Systems - cytology ; Neurosecretory Systems - metabolism ; Pituitary Gland - cytology ; Pituitary Gland - metabolism ; Pituitary Gland - ultrastructure ; Swine ; Tissue Fixation</subject><ispartof>Endocrinology (Philadelphia), 2002-03, Vol.143 (3), p.1144-1148</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c290t-542b3233d888a0382e14c92d2016cffb82cfac0c836c02c423906c69c3f332a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11861542$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cho, Sang-Joon</creatorcontrib><creatorcontrib>Jeftinija, Ksenija</creatorcontrib><creatorcontrib>Glavaski, Aleksandra</creatorcontrib><creatorcontrib>Jeftinija, Srdija</creatorcontrib><creatorcontrib>Jena, Bhanu P</creatorcontrib><creatorcontrib>Anderson, Lloyd L</creatorcontrib><title>Structure and dynamics of the fusion pores in live GH-secreting cells revealed using atomic force microscopy</title><title>Endocrinology (Philadelphia)</title><addtitle>Endocrinology</addtitle><description>Earlier studies in live pancreatic acinar cells identified new cellular structures at the cell plasma membrane called 'pits' and 'depressions', where membrane-bound secretory vesicles dock and fuse to release vesicular contents. In the current study, using atomic force microscopy we identify similar structures at the plasma membrane of GH-secreting cells of the pituitary and implicate their involvement in hormone release. Pits containing 100-200 nm in diameter depressions or fusion pores were identified in resting GH-secreting cells. Following stimulation of secretion the size of depression enlarged and gold-tagged GH antibody were found to bind to the pit structures in the stimulated GH cells. This study documents for the first time the presence of these structures and their involvement in secretions in a neuroendocrine cell.</description><subject>Actins - metabolism</subject><subject>Animals</subject><subject>Cell Separation</subject><subject>Growth Hormone - metabolism</subject><subject>Hemolytic Plaque Technique</subject><subject>Immunohistochemistry</subject><subject>Microscopy, Atomic Force</subject><subject>Neurosecretory Systems - cytology</subject><subject>Neurosecretory Systems - metabolism</subject><subject>Pituitary Gland - cytology</subject><subject>Pituitary Gland - metabolism</subject><subject>Pituitary Gland - ultrastructure</subject><subject>Swine</subject><subject>Tissue Fixation</subject><issn>0013-7227</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kDFPwzAQhT2AaCmMrMgTW4LtcxNnRBW0SJUY6B45lzMEJXGwk0r99wRRpnf39Onp6TF2J0UqlRSP1KdSQwqplFpfsKUQEpJcqXzBrmP8ml-tNVyxhZQmk2utlqx9H8OE4xSI277m9am3XYORe8fHT-Juio3v-eADRd70vG2OxLe7JBIGGpv-gyO1beSBjmRbqvnMz6Yd_ZzCnQ9IfL6Cj-iH0w27dLaNdHvWFTu8PB82u2T_tn3dPO0TVIUYk7lYBQqgNsZYAUaR1FioWgmZoXOVUegsCjSQoVCoFRQiw6xAcADKwoo9_MUOwX9PFMeya-JvT9uTn2KZy7UyOaxn8P4MTlVHdTmEprPhVP7PAz_1u2We</recordid><startdate>20020301</startdate><enddate>20020301</enddate><creator>Cho, Sang-Joon</creator><creator>Jeftinija, Ksenija</creator><creator>Glavaski, Aleksandra</creator><creator>Jeftinija, Srdija</creator><creator>Jena, Bhanu P</creator><creator>Anderson, Lloyd L</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20020301</creationdate><title>Structure and dynamics of the fusion pores in live GH-secreting cells revealed using atomic force microscopy</title><author>Cho, Sang-Joon ; Jeftinija, Ksenija ; Glavaski, Aleksandra ; Jeftinija, Srdija ; Jena, Bhanu P ; Anderson, Lloyd L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c290t-542b3233d888a0382e14c92d2016cffb82cfac0c836c02c423906c69c3f332a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Actins - metabolism</topic><topic>Animals</topic><topic>Cell Separation</topic><topic>Growth Hormone - metabolism</topic><topic>Hemolytic Plaque Technique</topic><topic>Immunohistochemistry</topic><topic>Microscopy, Atomic Force</topic><topic>Neurosecretory Systems - cytology</topic><topic>Neurosecretory Systems - metabolism</topic><topic>Pituitary Gland - cytology</topic><topic>Pituitary Gland - metabolism</topic><topic>Pituitary Gland - ultrastructure</topic><topic>Swine</topic><topic>Tissue Fixation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cho, Sang-Joon</creatorcontrib><creatorcontrib>Jeftinija, Ksenija</creatorcontrib><creatorcontrib>Glavaski, Aleksandra</creatorcontrib><creatorcontrib>Jeftinija, Srdija</creatorcontrib><creatorcontrib>Jena, Bhanu P</creatorcontrib><creatorcontrib>Anderson, Lloyd L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Endocrinology (Philadelphia)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cho, Sang-Joon</au><au>Jeftinija, Ksenija</au><au>Glavaski, Aleksandra</au><au>Jeftinija, Srdija</au><au>Jena, Bhanu P</au><au>Anderson, Lloyd L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structure and dynamics of the fusion pores in live GH-secreting cells revealed using atomic force microscopy</atitle><jtitle>Endocrinology (Philadelphia)</jtitle><addtitle>Endocrinology</addtitle><date>2002-03-01</date><risdate>2002</risdate><volume>143</volume><issue>3</issue><spage>1144</spage><epage>1148</epage><pages>1144-1148</pages><issn>0013-7227</issn><abstract>Earlier studies in live pancreatic acinar cells identified new cellular structures at the cell plasma membrane called 'pits' and 'depressions', where membrane-bound secretory vesicles dock and fuse to release vesicular contents. In the current study, using atomic force microscopy we identify similar structures at the plasma membrane of GH-secreting cells of the pituitary and implicate their involvement in hormone release. Pits containing 100-200 nm in diameter depressions or fusion pores were identified in resting GH-secreting cells. Following stimulation of secretion the size of depression enlarged and gold-tagged GH antibody were found to bind to the pit structures in the stimulated GH cells. This study documents for the first time the presence of these structures and their involvement in secretions in a neuroendocrine cell.</abstract><cop>United States</cop><pmid>11861542</pmid><doi>10.1210/en.143.3.1144</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0013-7227 |
| ispartof | Endocrinology (Philadelphia), 2002-03, Vol.143 (3), p.1144-1148 |
| issn | 0013-7227 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71528735 |
| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | Actins - metabolism Animals Cell Separation Growth Hormone - metabolism Hemolytic Plaque Technique Immunohistochemistry Microscopy, Atomic Force Neurosecretory Systems - cytology Neurosecretory Systems - metabolism Pituitary Gland - cytology Pituitary Gland - metabolism Pituitary Gland - ultrastructure Swine Tissue Fixation |
| title | Structure and dynamics of the fusion pores in live GH-secreting cells revealed using atomic force microscopy |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-22T22%3A15%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Structure%20and%20dynamics%20of%20the%20fusion%20pores%20in%20live%20GH-secreting%20cells%20revealed%20using%20atomic%20force%20microscopy&rft.jtitle=Endocrinology%20(Philadelphia)&rft.au=Cho,%20Sang-Joon&rft.date=2002-03-01&rft.volume=143&rft.issue=3&rft.spage=1144&rft.epage=1148&rft.pages=1144-1148&rft.issn=0013-7227&rft_id=info:doi/10.1210/en.143.3.1144&rft_dat=%3Cproquest_pubme%3E71528735%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=71528735&rft_id=info:pmid/11861542&rfr_iscdi=true |