Migration of Vascular Smooth Muscle Cells Induced by Sphingosine 1-Phosphate and Related Lipids: Potential Role in the Angiogenic Response
The bioactive lipids sphingosine 1-phosphate (SPP), sphingosylphosphorylcholine, and lysophosphatidic acid play an important role in angiogenesis as a result of their effects on both the migration of endothelial cells (ECs) and the integrity of EC monolayers. Here we show that extremely low concentr...
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| Veröffentlicht in: | Experimental cell research 2002-04, Vol.274 (2), p.264-274 |
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| creator | Boguslawski, George Grogg, Jeremy R. Welch, Zachary Ciechanowicz, Sandra Sliva, Daniel Kovala, A.Thomas McGlynn, Patrick Brindley, David N. Rhoades, Rodney A. English, Denis |
| description | The bioactive lipids sphingosine 1-phosphate (SPP), sphingosylphosphorylcholine, and lysophosphatidic acid play an important role in angiogenesis as a result of their effects on both the migration of endothelial cells (ECs) and the integrity of EC monolayers. Here we show that extremely low concentrations of serum and nanomolar concentrations of these biologically active lipids stimulate migration of human aortic smooth muscle cells (SMCs). However, at dosages most effective in promoting EC migration and in enhancing EC monolayer integrity, serum and SPP potently inhibited SMC migration; SPP also blocked the migration induced by protein growth factors. Treatment of SMCs with SPP induced transient phosphorylation of a 175- to 185-kDa protein corresponding to the PDGF receptor, indicating transactivation of this receptor. SPP and related lipids may play a key role in angiogenesis by coordinating the migration of both endothelial cells and vascular smooth muscle cells in response to the changing gradients of these bioactive lipid messengers. |
| doi_str_mv | 10.1006/excr.2002.5472 |
| format | Article |
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Here we show that extremely low concentrations of serum and nanomolar concentrations of these biologically active lipids stimulate migration of human aortic smooth muscle cells (SMCs). However, at dosages most effective in promoting EC migration and in enhancing EC monolayer integrity, serum and SPP potently inhibited SMC migration; SPP also blocked the migration induced by protein growth factors. Treatment of SMCs with SPP induced transient phosphorylation of a 175- to 185-kDa protein corresponding to the PDGF receptor, indicating transactivation of this receptor. SPP and related lipids may play a key role in angiogenesis by coordinating the migration of both endothelial cells and vascular smooth muscle cells in response to the changing gradients of these bioactive lipid messengers.</description><identifier>ISSN: 0014-4827</identifier><identifier>EISSN: 1090-2422</identifier><identifier>DOI: 10.1006/excr.2002.5472</identifier><identifier>PMID: 11900487</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>angiogenesis ; Blood Proteins - metabolism ; Blood Proteins - pharmacology ; Cell Communication - physiology ; Cell Movement - drug effects ; Cell Movement - physiology ; Cells, Cultured ; chemotaxis ; Chemotaxis - drug effects ; Chemotaxis - physiology ; Edg receptors ; Endothelium - cytology ; Endothelium - drug effects ; Endothelium - metabolism ; Growth Substances - pharmacology ; Humans ; lipid messengers ; Lysophospholipids ; Muscle, Smooth, Vascular - cytology ; Muscle, Smooth, Vascular - drug effects ; Muscle, Smooth, Vascular - metabolism ; Neovascularization, Physiologic - drug effects ; Neovascularization, Physiologic - physiology ; PDGF ; Phospholipids - metabolism ; Phospholipids - pharmacology ; Phosphorylation - drug effects ; Receptor, Epidermal Growth Factor - drug effects ; Receptor, Epidermal Growth Factor - metabolism ; Receptors, Platelet-Derived Growth Factor - drug effects ; Receptors, Platelet-Derived Growth Factor - metabolism ; serum ; Signal Transduction - drug effects ; Signal Transduction - physiology ; smooth muscle cell ; Sphingosine - analogs & derivatives ; Sphingosine - metabolism ; Sphingosine - pharmacology ; sphingosine 1-phosphate ; Virulence Factors, Bordetella - pharmacology</subject><ispartof>Experimental cell research, 2002-04, Vol.274 (2), p.264-274</ispartof><rights>2002 Elsevier Science (USA)</rights><rights>Copyright 2002 Elsevier Science (USA).</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-2629e10c16a1b6ce43a1377451ed9d3b249e8b94b864c1c2494222d8559755133</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/excr.2002.5472$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11900487$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Boguslawski, George</creatorcontrib><creatorcontrib>Grogg, Jeremy R.</creatorcontrib><creatorcontrib>Welch, Zachary</creatorcontrib><creatorcontrib>Ciechanowicz, Sandra</creatorcontrib><creatorcontrib>Sliva, Daniel</creatorcontrib><creatorcontrib>Kovala, A.Thomas</creatorcontrib><creatorcontrib>McGlynn, Patrick</creatorcontrib><creatorcontrib>Brindley, David N.</creatorcontrib><creatorcontrib>Rhoades, Rodney A.</creatorcontrib><creatorcontrib>English, Denis</creatorcontrib><title>Migration of Vascular Smooth Muscle Cells Induced by Sphingosine 1-Phosphate and Related Lipids: Potential Role in the Angiogenic Response</title><title>Experimental cell research</title><addtitle>Exp Cell Res</addtitle><description>The bioactive lipids sphingosine 1-phosphate (SPP), sphingosylphosphorylcholine, and lysophosphatidic acid play an important role in angiogenesis as a result of their effects on both the migration of endothelial cells (ECs) and the integrity of EC monolayers. Here we show that extremely low concentrations of serum and nanomolar concentrations of these biologically active lipids stimulate migration of human aortic smooth muscle cells (SMCs). However, at dosages most effective in promoting EC migration and in enhancing EC monolayer integrity, serum and SPP potently inhibited SMC migration; SPP also blocked the migration induced by protein growth factors. Treatment of SMCs with SPP induced transient phosphorylation of a 175- to 185-kDa protein corresponding to the PDGF receptor, indicating transactivation of this receptor. SPP and related lipids may play a key role in angiogenesis by coordinating the migration of both endothelial cells and vascular smooth muscle cells in response to the changing gradients of these bioactive lipid messengers.</description><subject>angiogenesis</subject><subject>Blood Proteins - metabolism</subject><subject>Blood Proteins - pharmacology</subject><subject>Cell Communication - physiology</subject><subject>Cell Movement - drug effects</subject><subject>Cell Movement - physiology</subject><subject>Cells, Cultured</subject><subject>chemotaxis</subject><subject>Chemotaxis - drug effects</subject><subject>Chemotaxis - physiology</subject><subject>Edg receptors</subject><subject>Endothelium - cytology</subject><subject>Endothelium - drug effects</subject><subject>Endothelium - metabolism</subject><subject>Growth Substances - pharmacology</subject><subject>Humans</subject><subject>lipid messengers</subject><subject>Lysophospholipids</subject><subject>Muscle, Smooth, Vascular - cytology</subject><subject>Muscle, Smooth, Vascular - drug effects</subject><subject>Muscle, Smooth, Vascular - metabolism</subject><subject>Neovascularization, Physiologic - drug effects</subject><subject>Neovascularization, Physiologic - physiology</subject><subject>PDGF</subject><subject>Phospholipids - metabolism</subject><subject>Phospholipids - pharmacology</subject><subject>Phosphorylation - drug effects</subject><subject>Receptor, Epidermal Growth Factor - drug effects</subject><subject>Receptor, Epidermal Growth Factor - metabolism</subject><subject>Receptors, Platelet-Derived Growth Factor - drug effects</subject><subject>Receptors, Platelet-Derived Growth Factor - metabolism</subject><subject>serum</subject><subject>Signal Transduction - drug effects</subject><subject>Signal Transduction - physiology</subject><subject>smooth muscle cell</subject><subject>Sphingosine - analogs & derivatives</subject><subject>Sphingosine - metabolism</subject><subject>Sphingosine - pharmacology</subject><subject>sphingosine 1-phosphate</subject><subject>Virulence Factors, Bordetella - pharmacology</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1v1DAQhi0EotvClSPyiVu2HsdJHG7Vio9KW1G1wNVy7NmNUdYOtoPoX-BX49WuxInTzEjP-0rzEPIG2BoYa6_xt4lrzhhfN6Ljz8gKWM8qLjh_TlaMgaiE5N0FuUzpB2NMSmhfkguAnjEhuxX5c-f2UWcXPA07-l0ns0w60sdDCHmkd0syE9INTlOit94uBi0dnujjPDq_D8l5pFDdjyHNo85Itbf0AaeyWrp1s7PpPb0PGX12eqIPoXQ5T_OI9MbvXdijd6YE0hx8wlfkxU5PCV-f5xX59vHD183navvl0-3mZlsZwWSueMt7BGag1TC0BkWtoe460QDa3tYDFz3KoReDbIUBU84ig1vZNH3XNFDXV-TdqXeO4eeCKauDS6a8qD2GJakOGt5JAQVcn0ATQ0oRd2qO7qDjkwKmjvbV0b462ldH-yXw9ty8DAe0__Cz7gLIE4Dlv18Oo0rGoS9WXUSTlQ3uf91_AZ1sk7o</recordid><startdate>20020401</startdate><enddate>20020401</enddate><creator>Boguslawski, George</creator><creator>Grogg, Jeremy R.</creator><creator>Welch, Zachary</creator><creator>Ciechanowicz, Sandra</creator><creator>Sliva, Daniel</creator><creator>Kovala, A.Thomas</creator><creator>McGlynn, Patrick</creator><creator>Brindley, David N.</creator><creator>Rhoades, Rodney A.</creator><creator>English, Denis</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020401</creationdate><title>Migration of Vascular Smooth Muscle Cells Induced by Sphingosine 1-Phosphate and Related Lipids: Potential Role in the Angiogenic Response</title><author>Boguslawski, George ; Grogg, Jeremy R. ; Welch, Zachary ; Ciechanowicz, Sandra ; Sliva, Daniel ; Kovala, A.Thomas ; McGlynn, Patrick ; Brindley, David N. ; Rhoades, Rodney A. ; English, Denis</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-2629e10c16a1b6ce43a1377451ed9d3b249e8b94b864c1c2494222d8559755133</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>angiogenesis</topic><topic>Blood Proteins - metabolism</topic><topic>Blood Proteins - pharmacology</topic><topic>Cell Communication - physiology</topic><topic>Cell Movement - drug effects</topic><topic>Cell Movement - physiology</topic><topic>Cells, Cultured</topic><topic>chemotaxis</topic><topic>Chemotaxis - drug effects</topic><topic>Chemotaxis - physiology</topic><topic>Edg receptors</topic><topic>Endothelium - cytology</topic><topic>Endothelium - drug effects</topic><topic>Endothelium - metabolism</topic><topic>Growth Substances - pharmacology</topic><topic>Humans</topic><topic>lipid messengers</topic><topic>Lysophospholipids</topic><topic>Muscle, Smooth, Vascular - cytology</topic><topic>Muscle, Smooth, Vascular - drug effects</topic><topic>Muscle, Smooth, Vascular - metabolism</topic><topic>Neovascularization, Physiologic - drug effects</topic><topic>Neovascularization, Physiologic - physiology</topic><topic>PDGF</topic><topic>Phospholipids - metabolism</topic><topic>Phospholipids - pharmacology</topic><topic>Phosphorylation - drug effects</topic><topic>Receptor, Epidermal Growth Factor - drug effects</topic><topic>Receptor, Epidermal Growth Factor - metabolism</topic><topic>Receptors, Platelet-Derived Growth Factor - drug effects</topic><topic>Receptors, Platelet-Derived Growth Factor - metabolism</topic><topic>serum</topic><topic>Signal Transduction - drug effects</topic><topic>Signal Transduction - physiology</topic><topic>smooth muscle cell</topic><topic>Sphingosine - analogs & derivatives</topic><topic>Sphingosine - metabolism</topic><topic>Sphingosine - pharmacology</topic><topic>sphingosine 1-phosphate</topic><topic>Virulence Factors, Bordetella - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Boguslawski, George</creatorcontrib><creatorcontrib>Grogg, Jeremy R.</creatorcontrib><creatorcontrib>Welch, Zachary</creatorcontrib><creatorcontrib>Ciechanowicz, Sandra</creatorcontrib><creatorcontrib>Sliva, Daniel</creatorcontrib><creatorcontrib>Kovala, A.Thomas</creatorcontrib><creatorcontrib>McGlynn, Patrick</creatorcontrib><creatorcontrib>Brindley, David N.</creatorcontrib><creatorcontrib>Rhoades, Rodney A.</creatorcontrib><creatorcontrib>English, Denis</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boguslawski, George</au><au>Grogg, Jeremy R.</au><au>Welch, Zachary</au><au>Ciechanowicz, Sandra</au><au>Sliva, Daniel</au><au>Kovala, A.Thomas</au><au>McGlynn, Patrick</au><au>Brindley, David N.</au><au>Rhoades, Rodney A.</au><au>English, Denis</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Migration of Vascular Smooth Muscle Cells Induced by Sphingosine 1-Phosphate and Related Lipids: Potential Role in the Angiogenic Response</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>2002-04-01</date><risdate>2002</risdate><volume>274</volume><issue>2</issue><spage>264</spage><epage>274</epage><pages>264-274</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><abstract>The bioactive lipids sphingosine 1-phosphate (SPP), sphingosylphosphorylcholine, and lysophosphatidic acid play an important role in angiogenesis as a result of their effects on both the migration of endothelial cells (ECs) and the integrity of EC monolayers. Here we show that extremely low concentrations of serum and nanomolar concentrations of these biologically active lipids stimulate migration of human aortic smooth muscle cells (SMCs). However, at dosages most effective in promoting EC migration and in enhancing EC monolayer integrity, serum and SPP potently inhibited SMC migration; SPP also blocked the migration induced by protein growth factors. Treatment of SMCs with SPP induced transient phosphorylation of a 175- to 185-kDa protein corresponding to the PDGF receptor, indicating transactivation of this receptor. SPP and related lipids may play a key role in angiogenesis by coordinating the migration of both endothelial cells and vascular smooth muscle cells in response to the changing gradients of these bioactive lipid messengers.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>11900487</pmid><doi>10.1006/excr.2002.5472</doi><tpages>11</tpages></addata></record> |
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| ispartof | Experimental cell research, 2002-04, Vol.274 (2), p.264-274 |
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| subjects | angiogenesis Blood Proteins - metabolism Blood Proteins - pharmacology Cell Communication - physiology Cell Movement - drug effects Cell Movement - physiology Cells, Cultured chemotaxis Chemotaxis - drug effects Chemotaxis - physiology Edg receptors Endothelium - cytology Endothelium - drug effects Endothelium - metabolism Growth Substances - pharmacology Humans lipid messengers Lysophospholipids Muscle, Smooth, Vascular - cytology Muscle, Smooth, Vascular - drug effects Muscle, Smooth, Vascular - metabolism Neovascularization, Physiologic - drug effects Neovascularization, Physiologic - physiology PDGF Phospholipids - metabolism Phospholipids - pharmacology Phosphorylation - drug effects Receptor, Epidermal Growth Factor - drug effects Receptor, Epidermal Growth Factor - metabolism Receptors, Platelet-Derived Growth Factor - drug effects Receptors, Platelet-Derived Growth Factor - metabolism serum Signal Transduction - drug effects Signal Transduction - physiology smooth muscle cell Sphingosine - analogs & derivatives Sphingosine - metabolism Sphingosine - pharmacology sphingosine 1-phosphate Virulence Factors, Bordetella - pharmacology |
| title | Migration of Vascular Smooth Muscle Cells Induced by Sphingosine 1-Phosphate and Related Lipids: Potential Role in the Angiogenic Response |
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