Cloning and expression in Pichia pastoris of a blue mussel (Mytilus edulis) β‐mannanase gene

Using PCR, cloning and sequencing techniques, a 1.1‐kb complementary DNA fragment encoding for a β‐mannanase (mannan endo‐1,4‐β‐mannosidase, EC 3.2.1.78) has been identified in the digestive gland of blue mussel, Mytilus edulis. The cDNA sequence shows significant sequence identity to several β‐mann...

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