An Ultraviolet Spectrophotometric Assay for Measuring Lipase Activity Using Long-Chain Triacyglycerols from Aleurites fordii Seeds

In this study, we designed a specific, continuous, and sensitive UV spectrophotometric lipase assay using natural triacylglycerols (TAGs) from the Aleurites fordii seed oil (tung oil). α-Eleostearic acid (9,11,13-cis, trans,trans-octadecatrienoic acid) is the main fatty acid component (it accounts f...

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Veröffentlicht in:	Analytical biochemistry 2002-04, Vol.303 (1), p.17-24
Hauptverfasser:	Pencreac'h, Gaëlle, Graille, Jean, Pina, Michel, Verger, Robert
Format:	Artikel
Sprache:	eng
Schlagworte:	Ascomycota - enzymology Bacillus subtilis - enzymology bile salts Euphorbiaceae - chemistry Fatty Acids, Nonesterified - chemistry Humans Hydrolysis Kinetics Lipase - analysis Lipase - metabolism Pancreas - enzymology polyunsaturated fatty acid Recombinant Proteins - metabolism Seeds - chemistry Spectrophotometry, Ultraviolet - methods Taurocholic Acid - chemistry Taurocholic Acid - metabolism Thermomyces lanuginosa lipase Triglycerides - chemistry Triglycerides - metabolism tung oil α-eleostearic acid
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