Improvement of serological discrimination between herpesvirus-infected animals and animals vaccinated with marker vaccines
Control/eradication plans of bovine herpesvirus 1 (BHV1) and suid herpesvirus 1 (SHV1) infections involve vaccination with inactivated or attenuated gE-deleted marker vaccines and associated companion serological tests to discriminate naturally infected from vaccinated animals. Blocking or competiti...
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description | Control/eradication plans of bovine herpesvirus 1 (BHV1) and suid herpesvirus 1 (SHV1) infections involve vaccination with inactivated or attenuated gE-deleted marker vaccines and associated companion serological tests to discriminate naturally infected from vaccinated animals. Blocking or competitive enzyme-linked immunosorbent assays (ELISAs) have been designed for the detection of specific antibodies against BHV1 or SHV1 gE glycoprotein. The antigen source usually consists of a crude viral preparation in which gE is associated with other envelope glycoproteins.
Such assays suffer from a lack of specificity which is not due to serological cross-reactions with other pathogens. Interestingly, false-positive results occur with sera collected from multivaccinated cattle or pigs. After multivaccination with a marker vaccine, the binding of the conjugated monoclonal antibody used as a tracer, could be hampered by antibodies directed against the other viral glycoproteins.
In order to validate the steric hindrance hypothesis, a simple preadsorption of such samples was carried out with a preparation of antigen devoid of gE, prior to the blocking ELISA itself. The decrease in antibody concentrations against the major glycoproteins, clearly leads to a better discrimination between positive and negative samples; that is between infected and multivaccinated animals, without significant loss of sensitivity. This experiment confirms the steric hindrance hypothesis, therefore serum preadsorption could be an easy way to improve the specificity of currently available diagnostic tests. |
doi_str_mv | 10.1016/S0378-1135(01)00491-6 |
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Such assays suffer from a lack of specificity which is not due to serological cross-reactions with other pathogens. Interestingly, false-positive results occur with sera collected from multivaccinated cattle or pigs. After multivaccination with a marker vaccine, the binding of the conjugated monoclonal antibody used as a tracer, could be hampered by antibodies directed against the other viral glycoproteins.
In order to validate the steric hindrance hypothesis, a simple preadsorption of such samples was carried out with a preparation of antigen devoid of gE, prior to the blocking ELISA itself. The decrease in antibody concentrations against the major glycoproteins, clearly leads to a better discrimination between positive and negative samples; that is between infected and multivaccinated animals, without significant loss of sensitivity. This experiment confirms the steric hindrance hypothesis, therefore serum preadsorption could be an easy way to improve the specificity of currently available diagnostic tests.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/S0378-1135(01)00491-6</identifier><identifier>PMID: 11888690</identifier><identifier>CODEN: VMICDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Antigens, Viral - immunology ; Applied microbiology ; Biological and medical sciences ; Bovine herpesvirus 1 ; Cattle ; Cattle Diseases - blood ; Cattle Diseases - immunology ; Cattle Diseases - virology ; Diagnostic ; Enzyme-Linked Immunosorbent Assay - methods ; Enzyme-Linked Immunosorbent Assay - veterinary ; Fundamental and applied biological sciences. Psychology ; glycoprotein E ; Herpesviridae Infections - blood ; Herpesviridae Infections - immunology ; Herpesviridae Infections - virology ; Herpesvirus 1, Bovine - immunology ; Herpesvirus 1, Bovine - isolation & purification ; Herpesvirus 1, Suid - immunology ; Herpesvirus 1, Suid - isolation & purification ; Marker vaccine ; Microbiology ; Multivaccinated animals ; Serum preadsorption ; Suid herpesvirus 1 ; Swine ; Swine Diseases - blood ; Swine Diseases - immunology ; Swine Diseases - virology ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) ; Viral Proteins - immunology ; Viral Vaccines - immunology ; Virology</subject><ispartof>Veterinary microbiology, 2002-04, Vol.86 (1), p.59-68</ispartof><rights>2002 Elsevier Science B.V.</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-d6197041c9f5a59f175efbc017b3ff65ab0461271b3a669942f1bffa8e6a4983</citedby><cites>FETCH-LOGICAL-c422t-d6197041c9f5a59f175efbc017b3ff65ab0461271b3a669942f1bffa8e6a4983</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0378-1135(01)00491-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,780,784,789,790,3550,23930,23931,25140,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13541074$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11888690$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lehmann, D</creatorcontrib><creatorcontrib>Sodoyer, R</creatorcontrib><creatorcontrib>Leterme, S</creatorcontrib><creatorcontrib>Crevat, D</creatorcontrib><title>Improvement of serological discrimination between herpesvirus-infected animals and animals vaccinated with marker vaccines</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>Control/eradication plans of bovine herpesvirus 1 (BHV1) and suid herpesvirus 1 (SHV1) infections involve vaccination with inactivated or attenuated gE-deleted marker vaccines and associated companion serological tests to discriminate naturally infected from vaccinated animals. Blocking or competitive enzyme-linked immunosorbent assays (ELISAs) have been designed for the detection of specific antibodies against BHV1 or SHV1 gE glycoprotein. The antigen source usually consists of a crude viral preparation in which gE is associated with other envelope glycoproteins.
Such assays suffer from a lack of specificity which is not due to serological cross-reactions with other pathogens. Interestingly, false-positive results occur with sera collected from multivaccinated cattle or pigs. After multivaccination with a marker vaccine, the binding of the conjugated monoclonal antibody used as a tracer, could be hampered by antibodies directed against the other viral glycoproteins.
In order to validate the steric hindrance hypothesis, a simple preadsorption of such samples was carried out with a preparation of antigen devoid of gE, prior to the blocking ELISA itself. The decrease in antibody concentrations against the major glycoproteins, clearly leads to a better discrimination between positive and negative samples; that is between infected and multivaccinated animals, without significant loss of sensitivity. This experiment confirms the steric hindrance hypothesis, therefore serum preadsorption could be an easy way to improve the specificity of currently available diagnostic tests.</description><subject>Animals</subject><subject>Antigens, Viral - immunology</subject><subject>Applied microbiology</subject><subject>Biological and medical sciences</subject><subject>Bovine herpesvirus 1</subject><subject>Cattle</subject><subject>Cattle Diseases - blood</subject><subject>Cattle Diseases - immunology</subject><subject>Cattle Diseases - virology</subject><subject>Diagnostic</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Enzyme-Linked Immunosorbent Assay - veterinary</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>glycoprotein E</subject><subject>Herpesviridae Infections - blood</subject><subject>Herpesviridae Infections - immunology</subject><subject>Herpesviridae Infections - virology</subject><subject>Herpesvirus 1, Bovine - immunology</subject><subject>Herpesvirus 1, Bovine - isolation & purification</subject><subject>Herpesvirus 1, Suid - immunology</subject><subject>Herpesvirus 1, Suid - isolation & purification</subject><subject>Marker vaccine</subject><subject>Microbiology</subject><subject>Multivaccinated animals</subject><subject>Serum preadsorption</subject><subject>Suid herpesvirus 1</subject><subject>Swine</subject><subject>Swine Diseases - blood</subject><subject>Swine Diseases - immunology</subject><subject>Swine Diseases - virology</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><subject>Viral Proteins - immunology</subject><subject>Viral Vaccines - immunology</subject><subject>Virology</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1P3DAQhi3UCra0PwGUS6v2kHYm_khyQhXqBxJSD-VuOc4YDImz2NlF7a-vl426R05j2c_Ynvdh7AzhMwKqL7-B102JyOVHwE8AosVSHbEVNjUvKymqV2z1Hzlhb1K6hx2l4JidIDZNo1pYsb9X4zpOWxopzMXkikRxGqZbb81Q9D7Z6EcfzOynUHQ0PxGF4o7imtLWx00qfXBkZ-oLE_xohpTrYb011u6a8_GTn--K0cQHiss2pbfstcsYvVvqKbv5_u3m8md5_evH1eXX69KKqprLXmFbg0DbOmlk67CW5DoLWHfcOSVNB0JhVWPHjVJtKyqHnXOmIWVE2_BT9mF_bZ7zcUNp1mOei4bBBJo2Sdcoc1wcXwSx4aBQQQblHrRxSimS0-sck4l_NILeydHPcvQueQ2on-VolfvOlwc23Uj9oWuxkYH3C2BSFuCiCdanA8elQKhF5i72HOXYtp6iTtZTsNT7mHXofvIvfOUfBeGuJA</recordid><startdate>20020422</startdate><enddate>20020422</enddate><creator>Lehmann, D</creator><creator>Sodoyer, R</creator><creator>Leterme, S</creator><creator>Crevat, D</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20020422</creationdate><title>Improvement of serological discrimination between herpesvirus-infected animals and animals vaccinated with marker vaccines</title><author>Lehmann, D ; Sodoyer, R ; Leterme, S ; Crevat, D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-d6197041c9f5a59f175efbc017b3ff65ab0461271b3a669942f1bffa8e6a4983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Antigens, Viral - immunology</topic><topic>Applied microbiology</topic><topic>Biological and medical sciences</topic><topic>Bovine herpesvirus 1</topic><topic>Cattle</topic><topic>Cattle Diseases - blood</topic><topic>Cattle Diseases - immunology</topic><topic>Cattle Diseases - virology</topic><topic>Diagnostic</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Enzyme-Linked Immunosorbent Assay - veterinary</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>glycoprotein E</topic><topic>Herpesviridae Infections - blood</topic><topic>Herpesviridae Infections - immunology</topic><topic>Herpesviridae Infections - virology</topic><topic>Herpesvirus 1, Bovine - immunology</topic><topic>Herpesvirus 1, Bovine - isolation & purification</topic><topic>Herpesvirus 1, Suid - immunology</topic><topic>Herpesvirus 1, Suid - isolation & purification</topic><topic>Marker vaccine</topic><topic>Microbiology</topic><topic>Multivaccinated animals</topic><topic>Serum preadsorption</topic><topic>Suid herpesvirus 1</topic><topic>Swine</topic><topic>Swine Diseases - blood</topic><topic>Swine Diseases - immunology</topic><topic>Swine Diseases - virology</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</topic><topic>Viral Proteins - immunology</topic><topic>Viral Vaccines - immunology</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lehmann, D</creatorcontrib><creatorcontrib>Sodoyer, R</creatorcontrib><creatorcontrib>Leterme, S</creatorcontrib><creatorcontrib>Crevat, D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lehmann, D</au><au>Sodoyer, R</au><au>Leterme, S</au><au>Crevat, D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improvement of serological discrimination between herpesvirus-infected animals and animals vaccinated with marker vaccines</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>2002-04-22</date><risdate>2002</risdate><volume>86</volume><issue>1</issue><spage>59</spage><epage>68</epage><pages>59-68</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><coden>VMICDQ</coden><abstract>Control/eradication plans of bovine herpesvirus 1 (BHV1) and suid herpesvirus 1 (SHV1) infections involve vaccination with inactivated or attenuated gE-deleted marker vaccines and associated companion serological tests to discriminate naturally infected from vaccinated animals. Blocking or competitive enzyme-linked immunosorbent assays (ELISAs) have been designed for the detection of specific antibodies against BHV1 or SHV1 gE glycoprotein. The antigen source usually consists of a crude viral preparation in which gE is associated with other envelope glycoproteins.
Such assays suffer from a lack of specificity which is not due to serological cross-reactions with other pathogens. Interestingly, false-positive results occur with sera collected from multivaccinated cattle or pigs. After multivaccination with a marker vaccine, the binding of the conjugated monoclonal antibody used as a tracer, could be hampered by antibodies directed against the other viral glycoproteins.
In order to validate the steric hindrance hypothesis, a simple preadsorption of such samples was carried out with a preparation of antigen devoid of gE, prior to the blocking ELISA itself. The decrease in antibody concentrations against the major glycoproteins, clearly leads to a better discrimination between positive and negative samples; that is between infected and multivaccinated animals, without significant loss of sensitivity. This experiment confirms the steric hindrance hypothesis, therefore serum preadsorption could be an easy way to improve the specificity of currently available diagnostic tests.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>11888690</pmid><doi>10.1016/S0378-1135(01)00491-6</doi><tpages>10</tpages></addata></record> |
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subjects | Animals Antigens, Viral - immunology Applied microbiology Biological and medical sciences Bovine herpesvirus 1 Cattle Cattle Diseases - blood Cattle Diseases - immunology Cattle Diseases - virology Diagnostic Enzyme-Linked Immunosorbent Assay - methods Enzyme-Linked Immunosorbent Assay - veterinary Fundamental and applied biological sciences. Psychology glycoprotein E Herpesviridae Infections - blood Herpesviridae Infections - immunology Herpesviridae Infections - virology Herpesvirus 1, Bovine - immunology Herpesvirus 1, Bovine - isolation & purification Herpesvirus 1, Suid - immunology Herpesvirus 1, Suid - isolation & purification Marker vaccine Microbiology Multivaccinated animals Serum preadsorption Suid herpesvirus 1 Swine Swine Diseases - blood Swine Diseases - immunology Swine Diseases - virology Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Viral Proteins - immunology Viral Vaccines - immunology Virology |
title | Improvement of serological discrimination between herpesvirus-infected animals and animals vaccinated with marker vaccines |
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