Immunohistochemical screening of mismatch repair genes hMLH1, hMSH2, and hMSH6 in dysplastic lesions of the colon
Immunohistochemical decrease in staining for mismatch repair proteins may be seen in either microsatellite instability or inactivation (methylation) of mismatch repair proteins. Both are features of the malignant phenotype in a range of colorectal neoplasms. Expression of mismatch repair proteins in...
Gespeichert in:
| Veröffentlicht in: | Applied immunohistochemistry & molecular morphology 2002-03, Vol.10 (1), p.57-61 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 61 |
|---|---|
| container_issue | 1 |
| container_start_page | 57 |
| container_title | Applied immunohistochemistry & molecular morphology |
| container_volume | 10 |
| creator | Fogt, Franz Zimmerman, Robert L Poremba, Christopher Noffsinger, Amy E Alsaigh, Nada Rueschoff, Joseph |
| description | Immunohistochemical decrease in staining for mismatch repair proteins may be seen in either microsatellite instability or inactivation (methylation) of mismatch repair proteins. Both are features of the malignant phenotype in a range of colorectal neoplasms. Expression of mismatch repair proteins in dysplastic lesions in ulcerative colitis (UC) has not been studied extensively. The authors analyzed protein expression of hMLH1, hMSH2, and hMSH6 in 18 cases of dysplasia-associated lesion or mass (DALM) in patients with UC and 10 sporadic adenomas. Immunohistochemical studies revealed adequate staining in almost all of the cases. A significant decrease in protein expression was seen in 2 DALM and 2 sporadic adenomas. The authors conclude that immunohistochemical studies of mismatch repair proteins can be applied to dysplastic lesions in UC with adequate staining results. Decreased wild type expression of hMLH1, hMSH2, and hMSH6 is not a feature of DALM in the setting of UC. |
| doi_str_mv | 10.1097/00129039-200203000-00010 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71524404</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>21093264</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1897-c95d6ddca5560622f416a39ed309796f4832a5176f63db73eca2fa195a3c3a113</originalsourceid><addsrcrecordid>eNqFkU1PAyEQhjlobP34C4aTp64ywLLdo2nUNqnxoJ4JBbaL2YV22T3030s_1KMHMmR4550ZHoQwkHsgZfFACNCSsDKjhFDCCCFZOkDO0BhyDikNYoQuY_xK75RxfoFGANOSEVaM0XbRtoMPtYt90LVtnVYNjrqz1ju_xqHCrYut6nWNO7tRrsNr623E9etyDpMU3ud0gpU3h6vAzmOzi5tGxd5p3Njogo97m762WIcm-Gt0Xqkm2ptTvEKfz08fs3m2fHtZzB6XmU7DFZkucyOM0SrPBRGUVhyEYqU1LC1diopPGVU5FKISzKwKZrWilYIyV0wzBcCu0N3Rd9OF7WBjL9Mm2jaN8jYMURaQU84J_1dI0zczKvbC6VGouxBjZyu56Vyrup0EIvcs5A8L-ctCHlik0ttTj2HVWvNXeALBvgGlD4VW</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>21093264</pqid></control><display><type>article</type><title>Immunohistochemical screening of mismatch repair genes hMLH1, hMSH2, and hMSH6 in dysplastic lesions of the colon</title><source>MEDLINE</source><source>Journals@Ovid Complete</source><creator>Fogt, Franz ; Zimmerman, Robert L ; Poremba, Christopher ; Noffsinger, Amy E ; Alsaigh, Nada ; Rueschoff, Joseph</creator><creatorcontrib>Fogt, Franz ; Zimmerman, Robert L ; Poremba, Christopher ; Noffsinger, Amy E ; Alsaigh, Nada ; Rueschoff, Joseph</creatorcontrib><description>Immunohistochemical decrease in staining for mismatch repair proteins may be seen in either microsatellite instability or inactivation (methylation) of mismatch repair proteins. Both are features of the malignant phenotype in a range of colorectal neoplasms. Expression of mismatch repair proteins in dysplastic lesions in ulcerative colitis (UC) has not been studied extensively. The authors analyzed protein expression of hMLH1, hMSH2, and hMSH6 in 18 cases of dysplasia-associated lesion or mass (DALM) in patients with UC and 10 sporadic adenomas. Immunohistochemical studies revealed adequate staining in almost all of the cases. A significant decrease in protein expression was seen in 2 DALM and 2 sporadic adenomas. The authors conclude that immunohistochemical studies of mismatch repair proteins can be applied to dysplastic lesions in UC with adequate staining results. Decreased wild type expression of hMLH1, hMSH2, and hMSH6 is not a feature of DALM in the setting of UC.</description><identifier>ISSN: 1541-2016</identifier><identifier>DOI: 10.1097/00129039-200203000-00010</identifier><identifier>PMID: 11893037</identifier><language>eng</language><publisher>United States</publisher><subject>Adaptor Proteins, Signal Transducing ; Adult ; Aged ; Aged, 80 and over ; Base Pair Mismatch ; Carrier Proteins ; Case-Control Studies ; Colitis, Ulcerative - pathology ; Colonic Neoplasms - genetics ; DNA Repair - genetics ; DNA-Binding Proteins - genetics ; Female ; Humans ; Immunohistochemistry ; Male ; Middle Aged ; MutL Protein Homolog 1 ; MutS Homolog 2 Protein ; Neoplasm Proteins - genetics ; Nuclear Proteins ; Precancerous Conditions - genetics ; Proto-Oncogene Proteins - genetics</subject><ispartof>Applied immunohistochemistry & molecular morphology, 2002-03, Vol.10 (1), p.57-61</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1897-c95d6ddca5560622f416a39ed309796f4832a5176f63db73eca2fa195a3c3a113</citedby><cites>FETCH-LOGICAL-c1897-c95d6ddca5560622f416a39ed309796f4832a5176f63db73eca2fa195a3c3a113</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11893037$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fogt, Franz</creatorcontrib><creatorcontrib>Zimmerman, Robert L</creatorcontrib><creatorcontrib>Poremba, Christopher</creatorcontrib><creatorcontrib>Noffsinger, Amy E</creatorcontrib><creatorcontrib>Alsaigh, Nada</creatorcontrib><creatorcontrib>Rueschoff, Joseph</creatorcontrib><title>Immunohistochemical screening of mismatch repair genes hMLH1, hMSH2, and hMSH6 in dysplastic lesions of the colon</title><title>Applied immunohistochemistry & molecular morphology</title><addtitle>Appl Immunohistochem Mol Morphol</addtitle><description>Immunohistochemical decrease in staining for mismatch repair proteins may be seen in either microsatellite instability or inactivation (methylation) of mismatch repair proteins. Both are features of the malignant phenotype in a range of colorectal neoplasms. Expression of mismatch repair proteins in dysplastic lesions in ulcerative colitis (UC) has not been studied extensively. The authors analyzed protein expression of hMLH1, hMSH2, and hMSH6 in 18 cases of dysplasia-associated lesion or mass (DALM) in patients with UC and 10 sporadic adenomas. Immunohistochemical studies revealed adequate staining in almost all of the cases. A significant decrease in protein expression was seen in 2 DALM and 2 sporadic adenomas. The authors conclude that immunohistochemical studies of mismatch repair proteins can be applied to dysplastic lesions in UC with adequate staining results. Decreased wild type expression of hMLH1, hMSH2, and hMSH6 is not a feature of DALM in the setting of UC.</description><subject>Adaptor Proteins, Signal Transducing</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Base Pair Mismatch</subject><subject>Carrier Proteins</subject><subject>Case-Control Studies</subject><subject>Colitis, Ulcerative - pathology</subject><subject>Colonic Neoplasms - genetics</subject><subject>DNA Repair - genetics</subject><subject>DNA-Binding Proteins - genetics</subject><subject>Female</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Middle Aged</subject><subject>MutL Protein Homolog 1</subject><subject>MutS Homolog 2 Protein</subject><subject>Neoplasm Proteins - genetics</subject><subject>Nuclear Proteins</subject><subject>Precancerous Conditions - genetics</subject><subject>Proto-Oncogene Proteins - genetics</subject><issn>1541-2016</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1PAyEQhjlobP34C4aTp64ywLLdo2nUNqnxoJ4JBbaL2YV22T3030s_1KMHMmR4550ZHoQwkHsgZfFACNCSsDKjhFDCCCFZOkDO0BhyDikNYoQuY_xK75RxfoFGANOSEVaM0XbRtoMPtYt90LVtnVYNjrqz1ju_xqHCrYut6nWNO7tRrsNr623E9etyDpMU3ud0gpU3h6vAzmOzi5tGxd5p3Njogo97m762WIcm-Gt0Xqkm2ptTvEKfz08fs3m2fHtZzB6XmU7DFZkucyOM0SrPBRGUVhyEYqU1LC1diopPGVU5FKISzKwKZrWilYIyV0wzBcCu0N3Rd9OF7WBjL9Mm2jaN8jYMURaQU84J_1dI0zczKvbC6VGouxBjZyu56Vyrup0EIvcs5A8L-ctCHlik0ttTj2HVWvNXeALBvgGlD4VW</recordid><startdate>200203</startdate><enddate>200203</enddate><creator>Fogt, Franz</creator><creator>Zimmerman, Robert L</creator><creator>Poremba, Christopher</creator><creator>Noffsinger, Amy E</creator><creator>Alsaigh, Nada</creator><creator>Rueschoff, Joseph</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200203</creationdate><title>Immunohistochemical screening of mismatch repair genes hMLH1, hMSH2, and hMSH6 in dysplastic lesions of the colon</title><author>Fogt, Franz ; Zimmerman, Robert L ; Poremba, Christopher ; Noffsinger, Amy E ; Alsaigh, Nada ; Rueschoff, Joseph</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1897-c95d6ddca5560622f416a39ed309796f4832a5176f63db73eca2fa195a3c3a113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adaptor Proteins, Signal Transducing</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Base Pair Mismatch</topic><topic>Carrier Proteins</topic><topic>Case-Control Studies</topic><topic>Colitis, Ulcerative - pathology</topic><topic>Colonic Neoplasms - genetics</topic><topic>DNA Repair - genetics</topic><topic>DNA-Binding Proteins - genetics</topic><topic>Female</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Middle Aged</topic><topic>MutL Protein Homolog 1</topic><topic>MutS Homolog 2 Protein</topic><topic>Neoplasm Proteins - genetics</topic><topic>Nuclear Proteins</topic><topic>Precancerous Conditions - genetics</topic><topic>Proto-Oncogene Proteins - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fogt, Franz</creatorcontrib><creatorcontrib>Zimmerman, Robert L</creatorcontrib><creatorcontrib>Poremba, Christopher</creatorcontrib><creatorcontrib>Noffsinger, Amy E</creatorcontrib><creatorcontrib>Alsaigh, Nada</creatorcontrib><creatorcontrib>Rueschoff, Joseph</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Applied immunohistochemistry & molecular morphology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fogt, Franz</au><au>Zimmerman, Robert L</au><au>Poremba, Christopher</au><au>Noffsinger, Amy E</au><au>Alsaigh, Nada</au><au>Rueschoff, Joseph</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunohistochemical screening of mismatch repair genes hMLH1, hMSH2, and hMSH6 in dysplastic lesions of the colon</atitle><jtitle>Applied immunohistochemistry & molecular morphology</jtitle><addtitle>Appl Immunohistochem Mol Morphol</addtitle><date>2002-03</date><risdate>2002</risdate><volume>10</volume><issue>1</issue><spage>57</spage><epage>61</epage><pages>57-61</pages><issn>1541-2016</issn><abstract>Immunohistochemical decrease in staining for mismatch repair proteins may be seen in either microsatellite instability or inactivation (methylation) of mismatch repair proteins. Both are features of the malignant phenotype in a range of colorectal neoplasms. Expression of mismatch repair proteins in dysplastic lesions in ulcerative colitis (UC) has not been studied extensively. The authors analyzed protein expression of hMLH1, hMSH2, and hMSH6 in 18 cases of dysplasia-associated lesion or mass (DALM) in patients with UC and 10 sporadic adenomas. Immunohistochemical studies revealed adequate staining in almost all of the cases. A significant decrease in protein expression was seen in 2 DALM and 2 sporadic adenomas. The authors conclude that immunohistochemical studies of mismatch repair proteins can be applied to dysplastic lesions in UC with adequate staining results. Decreased wild type expression of hMLH1, hMSH2, and hMSH6 is not a feature of DALM in the setting of UC.</abstract><cop>United States</cop><pmid>11893037</pmid><doi>10.1097/00129039-200203000-00010</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1541-2016 |
| ispartof | Applied immunohistochemistry & molecular morphology, 2002-03, Vol.10 (1), p.57-61 |
| issn | 1541-2016 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71524404 |
| source | MEDLINE; Journals@Ovid Complete |
| subjects | Adaptor Proteins, Signal Transducing Adult Aged Aged, 80 and over Base Pair Mismatch Carrier Proteins Case-Control Studies Colitis, Ulcerative - pathology Colonic Neoplasms - genetics DNA Repair - genetics DNA-Binding Proteins - genetics Female Humans Immunohistochemistry Male Middle Aged MutL Protein Homolog 1 MutS Homolog 2 Protein Neoplasm Proteins - genetics Nuclear Proteins Precancerous Conditions - genetics Proto-Oncogene Proteins - genetics |
| title | Immunohistochemical screening of mismatch repair genes hMLH1, hMSH2, and hMSH6 in dysplastic lesions of the colon |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-26T17%3A55%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Immunohistochemical%20screening%20of%20mismatch%20repair%20genes%20hMLH1,%20hMSH2,%20and%20hMSH6%20in%20dysplastic%20lesions%20of%20the%20colon&rft.jtitle=Applied%20immunohistochemistry%20&%20molecular%20morphology&rft.au=Fogt,%20Franz&rft.date=2002-03&rft.volume=10&rft.issue=1&rft.spage=57&rft.epage=61&rft.pages=57-61&rft.issn=1541-2016&rft_id=info:doi/10.1097/00129039-200203000-00010&rft_dat=%3Cproquest_cross%3E21093264%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=21093264&rft_id=info:pmid/11893037&rfr_iscdi=true |