Cellular and Molecular Alterations in Spinal Cord Injury Patients with Pressure Ulcers: A Preliminary Report
The study was designed to investigate the changes, both numerically and functionally, of the molecules critical to wound healing in spinal cord injury (SCI) patients. Spinal cord injury patients who demonstrated delayed healing of their pressure ulcers were used as study subjects. Age-matched health...
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| Veröffentlicht in: | Experimental and molecular pathology 2002-04, Vol.72 (2), p.124-131 |
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| creator | Cruse, J.M. Wang, H. Lewis, R.E. Cespedes, J. Morrison, R.S. Lineaweaver, W.C. Dilioglou, S. |
| description | The study was designed to investigate the changes, both numerically and functionally, of the molecules critical to wound healing in spinal cord injury (SCI) patients. Spinal cord injury patients who demonstrated delayed healing of their pressure ulcers were used as study subjects. Age-matched healthy individuals served as controls. Adhesion molecule expression of the peripheral blood leukocytes, including lymphocytes and granulocytes, was measured by flow cytometric analysis. Binding capacity of the lymphocytes was evaluated using human umbilical cord vein endothelial cells (HUVECs) as the binding matrix. Samples from pressure ulcers of the patients were immunostained to define fibronectin, kalinin, β4 integrin, α2β1, α3β1, α5β1, and CD138 expression. Compared to healthy controls, there was decreased expression of CD11a, CD11b, CD18, CD49b, CD49c, CD49d, CD54, and CD8 in patients' lymphocyte populations and CD11a, CD18, CD49c, CD49d, and CD8 in patients' granulocyte populations. The binding capacity, expressed as percentage binding of the lymphocytes to the HUVEC matrix, was greatly diminished in the patients. There was markedly diminished immunohistochemical staining of fibronectin in pressure ulcers. These findings showed that delayed healing of pressure ulcers in SCI patients can be attributed to reduced adhesion molecule expression, impaired cell—cell interaction, and lack of extracellular matrix structural and functional protein. |
| doi_str_mv | 10.1006/exmp.2002.2420 |
| format | Article |
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Spinal cord injury patients who demonstrated delayed healing of their pressure ulcers were used as study subjects. Age-matched healthy individuals served as controls. Adhesion molecule expression of the peripheral blood leukocytes, including lymphocytes and granulocytes, was measured by flow cytometric analysis. Binding capacity of the lymphocytes was evaluated using human umbilical cord vein endothelial cells (HUVECs) as the binding matrix. Samples from pressure ulcers of the patients were immunostained to define fibronectin, kalinin, β4 integrin, α2β1, α3β1, α5β1, and CD138 expression. Compared to healthy controls, there was decreased expression of CD11a, CD11b, CD18, CD49b, CD49c, CD49d, CD54, and CD8 in patients' lymphocyte populations and CD11a, CD18, CD49c, CD49d, and CD8 in patients' granulocyte populations. The binding capacity, expressed as percentage binding of the lymphocytes to the HUVEC matrix, was greatly diminished in the patients. There was markedly diminished immunohistochemical staining of fibronectin in pressure ulcers. These findings showed that delayed healing of pressure ulcers in SCI patients can be attributed to reduced adhesion molecule expression, impaired cell—cell interaction, and lack of extracellular matrix structural and functional protein.</description><identifier>ISSN: 0014-4800</identifier><identifier>EISSN: 1096-0945</identifier><identifier>DOI: 10.1006/exmp.2002.2420</identifier><identifier>PMID: 11890721</identifier><identifier>CODEN: EXMPA6</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Adult ; Antigens, CD - metabolism ; Biological and medical sciences ; Cell Adhesion Molecules - blood ; cellular adhesion molecules ; Dermatology ; Endothelium, Vascular - cytology ; Endothelium, Vascular - metabolism ; Extracellular Matrix - metabolism ; fibronectin ; Fibronectins - metabolism ; Flow Cytometry ; Granulocytes - metabolism ; Granulocytes - pathology ; Humans ; Immunoenzyme Techniques ; Injuries of the nervous system and the skull. Diseases due to physical agents ; Medical sciences ; Monocytes - metabolism ; Monocytes - pathology ; Pressure Ulcer - blood ; Pressure Ulcer - etiology ; Pressure Ulcer - pathology ; pressure ulcers ; Skin involvement in other diseases. Miscellaneous. General aspects ; Spinal Cord Injuries - blood ; Spinal Cord Injuries - complications ; Spinal Cord Injuries - pathology ; spinal cord injury ; Traumas. Diseases due to physical agents ; Umbilical Veins - cytology ; Umbilical Veins - metabolism ; Wound Healing</subject><ispartof>Experimental and molecular pathology, 2002-04, Vol.72 (2), p.124-131</ispartof><rights>2002 Elsevier Science (USA)</rights><rights>2002 INIST-CNRS</rights><rights>Copyright 2002 Elsevier Science (USA).</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-ab35520368e03e896d8e1af0dfad26a7e64eab3d47a7bb33dce752a50dc5124f3</citedby><cites>FETCH-LOGICAL-c370t-ab35520368e03e896d8e1af0dfad26a7e64eab3d47a7bb33dce752a50dc5124f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0014480002924202$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13605732$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11890721$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cruse, J.M.</creatorcontrib><creatorcontrib>Wang, H.</creatorcontrib><creatorcontrib>Lewis, R.E.</creatorcontrib><creatorcontrib>Cespedes, J.</creatorcontrib><creatorcontrib>Morrison, R.S.</creatorcontrib><creatorcontrib>Lineaweaver, W.C.</creatorcontrib><creatorcontrib>Dilioglou, S.</creatorcontrib><title>Cellular and Molecular Alterations in Spinal Cord Injury Patients with Pressure Ulcers: A Preliminary Report</title><title>Experimental and molecular pathology</title><addtitle>Exp Mol Pathol</addtitle><description>The study was designed to investigate the changes, both numerically and functionally, of the molecules critical to wound healing in spinal cord injury (SCI) patients. Spinal cord injury patients who demonstrated delayed healing of their pressure ulcers were used as study subjects. Age-matched healthy individuals served as controls. Adhesion molecule expression of the peripheral blood leukocytes, including lymphocytes and granulocytes, was measured by flow cytometric analysis. Binding capacity of the lymphocytes was evaluated using human umbilical cord vein endothelial cells (HUVECs) as the binding matrix. Samples from pressure ulcers of the patients were immunostained to define fibronectin, kalinin, β4 integrin, α2β1, α3β1, α5β1, and CD138 expression. Compared to healthy controls, there was decreased expression of CD11a, CD11b, CD18, CD49b, CD49c, CD49d, CD54, and CD8 in patients' lymphocyte populations and CD11a, CD18, CD49c, CD49d, and CD8 in patients' granulocyte populations. The binding capacity, expressed as percentage binding of the lymphocytes to the HUVEC matrix, was greatly diminished in the patients. There was markedly diminished immunohistochemical staining of fibronectin in pressure ulcers. These findings showed that delayed healing of pressure ulcers in SCI patients can be attributed to reduced adhesion molecule expression, impaired cell—cell interaction, and lack of extracellular matrix structural and functional protein.</description><subject>Adult</subject><subject>Antigens, CD - metabolism</subject><subject>Biological and medical sciences</subject><subject>Cell Adhesion Molecules - blood</subject><subject>cellular adhesion molecules</subject><subject>Dermatology</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - metabolism</subject><subject>Extracellular Matrix - metabolism</subject><subject>fibronectin</subject><subject>Fibronectins - metabolism</subject><subject>Flow Cytometry</subject><subject>Granulocytes - metabolism</subject><subject>Granulocytes - pathology</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Injuries of the nervous system and the skull. Diseases due to physical agents</subject><subject>Medical sciences</subject><subject>Monocytes - metabolism</subject><subject>Monocytes - pathology</subject><subject>Pressure Ulcer - blood</subject><subject>Pressure Ulcer - etiology</subject><subject>Pressure Ulcer - pathology</subject><subject>pressure ulcers</subject><subject>Skin involvement in other diseases. Miscellaneous. General aspects</subject><subject>Spinal Cord Injuries - blood</subject><subject>Spinal Cord Injuries - complications</subject><subject>Spinal Cord Injuries - pathology</subject><subject>spinal cord injury</subject><subject>Traumas. Diseases due to physical agents</subject><subject>Umbilical Veins - cytology</subject><subject>Umbilical Veins - metabolism</subject><subject>Wound Healing</subject><issn>0014-4800</issn><issn>1096-0945</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kEtPGzEQgC1UVNK0V47Il3LbMLb32VsUAY0EIoJythx7VjjyPmrvFvj3eJtIOXEazcw3o5mPkHMGCwaQX-Fb0y84AF_wlMMJmTGo8gSqNPtCZgAsTdIS4Ix8C2EHABUw_pWcMVZWUHA2I26Fzo1OeapaQ-87h_p_tnQDejXYrg3UtvSpt61ydNV5Q9ftbvTvdBO72A6BvtrhhW48hjB6pM9Oow-_6HIqOdvEuQg_Yt_54Ts5rZUL-OMQ5-T55vrP6ndy93C7Xi3vEi0KGBK1FVnGQeQlgsCyyk2JTNVgamV4rgrMU4yMSQtVbLdCGI1FxlUGRmeMp7WYk8v93t53f0cMg2xs0PFR1WI3BlmwjOVVWUZwsQe170LwWMve2yYeLBnIya-c_MrJr5z8xoGLw-Zx26A54gehEfh5AFTQytVetdqGIydyyArBI1fuOYwe_ln0MuioU6OxHvUgTWc_u-EDcZSX-w</recordid><startdate>20020401</startdate><enddate>20020401</enddate><creator>Cruse, J.M.</creator><creator>Wang, H.</creator><creator>Lewis, R.E.</creator><creator>Cespedes, J.</creator><creator>Morrison, R.S.</creator><creator>Lineaweaver, W.C.</creator><creator>Dilioglou, S.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020401</creationdate><title>Cellular and Molecular Alterations in Spinal Cord Injury Patients with Pressure Ulcers: A Preliminary Report</title><author>Cruse, J.M. ; Wang, H. ; Lewis, R.E. ; Cespedes, J. ; Morrison, R.S. ; Lineaweaver, W.C. ; Dilioglou, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-ab35520368e03e896d8e1af0dfad26a7e64eab3d47a7bb33dce752a50dc5124f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adult</topic><topic>Antigens, CD - metabolism</topic><topic>Biological and medical sciences</topic><topic>Cell Adhesion Molecules - blood</topic><topic>cellular adhesion molecules</topic><topic>Dermatology</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - metabolism</topic><topic>Extracellular Matrix - metabolism</topic><topic>fibronectin</topic><topic>Fibronectins - metabolism</topic><topic>Flow Cytometry</topic><topic>Granulocytes - metabolism</topic><topic>Granulocytes - pathology</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>Injuries of the nervous system and the skull. Diseases due to physical agents</topic><topic>Medical sciences</topic><topic>Monocytes - metabolism</topic><topic>Monocytes - pathology</topic><topic>Pressure Ulcer - blood</topic><topic>Pressure Ulcer - etiology</topic><topic>Pressure Ulcer - pathology</topic><topic>pressure ulcers</topic><topic>Skin involvement in other diseases. Miscellaneous. General aspects</topic><topic>Spinal Cord Injuries - blood</topic><topic>Spinal Cord Injuries - complications</topic><topic>Spinal Cord Injuries - pathology</topic><topic>spinal cord injury</topic><topic>Traumas. Diseases due to physical agents</topic><topic>Umbilical Veins - cytology</topic><topic>Umbilical Veins - metabolism</topic><topic>Wound Healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cruse, J.M.</creatorcontrib><creatorcontrib>Wang, H.</creatorcontrib><creatorcontrib>Lewis, R.E.</creatorcontrib><creatorcontrib>Cespedes, J.</creatorcontrib><creatorcontrib>Morrison, R.S.</creatorcontrib><creatorcontrib>Lineaweaver, W.C.</creatorcontrib><creatorcontrib>Dilioglou, S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental and molecular pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cruse, J.M.</au><au>Wang, H.</au><au>Lewis, R.E.</au><au>Cespedes, J.</au><au>Morrison, R.S.</au><au>Lineaweaver, W.C.</au><au>Dilioglou, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cellular and Molecular Alterations in Spinal Cord Injury Patients with Pressure Ulcers: A Preliminary Report</atitle><jtitle>Experimental and molecular pathology</jtitle><addtitle>Exp Mol Pathol</addtitle><date>2002-04-01</date><risdate>2002</risdate><volume>72</volume><issue>2</issue><spage>124</spage><epage>131</epage><pages>124-131</pages><issn>0014-4800</issn><eissn>1096-0945</eissn><coden>EXMPA6</coden><abstract>The study was designed to investigate the changes, both numerically and functionally, of the molecules critical to wound healing in spinal cord injury (SCI) patients. Spinal cord injury patients who demonstrated delayed healing of their pressure ulcers were used as study subjects. Age-matched healthy individuals served as controls. Adhesion molecule expression of the peripheral blood leukocytes, including lymphocytes and granulocytes, was measured by flow cytometric analysis. Binding capacity of the lymphocytes was evaluated using human umbilical cord vein endothelial cells (HUVECs) as the binding matrix. Samples from pressure ulcers of the patients were immunostained to define fibronectin, kalinin, β4 integrin, α2β1, α3β1, α5β1, and CD138 expression. Compared to healthy controls, there was decreased expression of CD11a, CD11b, CD18, CD49b, CD49c, CD49d, CD54, and CD8 in patients' lymphocyte populations and CD11a, CD18, CD49c, CD49d, and CD8 in patients' granulocyte populations. The binding capacity, expressed as percentage binding of the lymphocytes to the HUVEC matrix, was greatly diminished in the patients. There was markedly diminished immunohistochemical staining of fibronectin in pressure ulcers. These findings showed that delayed healing of pressure ulcers in SCI patients can be attributed to reduced adhesion molecule expression, impaired cell—cell interaction, and lack of extracellular matrix structural and functional protein.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>11890721</pmid><doi>10.1006/exmp.2002.2420</doi><tpages>8</tpages></addata></record> |
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| subjects | Adult Antigens, CD - metabolism Biological and medical sciences Cell Adhesion Molecules - blood cellular adhesion molecules Dermatology Endothelium, Vascular - cytology Endothelium, Vascular - metabolism Extracellular Matrix - metabolism fibronectin Fibronectins - metabolism Flow Cytometry Granulocytes - metabolism Granulocytes - pathology Humans Immunoenzyme Techniques Injuries of the nervous system and the skull. Diseases due to physical agents Medical sciences Monocytes - metabolism Monocytes - pathology Pressure Ulcer - blood Pressure Ulcer - etiology Pressure Ulcer - pathology pressure ulcers Skin involvement in other diseases. Miscellaneous. General aspects Spinal Cord Injuries - blood Spinal Cord Injuries - complications Spinal Cord Injuries - pathology spinal cord injury Traumas. Diseases due to physical agents Umbilical Veins - cytology Umbilical Veins - metabolism Wound Healing |
| title | Cellular and Molecular Alterations in Spinal Cord Injury Patients with Pressure Ulcers: A Preliminary Report |
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