Ribosomal RNA analysis of Babesia odocoilei isolates from farmed reindeer (Rangifer tarandus tarandus) and elk (Cervus elaphus canadensis) in Wisconsin
Piroplasms isolated from a farmed reindeer and elk in Wisconsin were determined to be Babesia odocoilei, based on morphology and ribosomal RNA (rRNA) analysis. Different clinical manifestations were observed in the two host species. The reindeer was parasitemic and exhibited acute babesiosis resulti...
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| Veröffentlicht in: | Parasitology research (1987) 2003-11, Vol.91 (5), p.378-383 |
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| container_title | Parasitology research (1987) |
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| creator | HOLMAN, Patricia J BENDELE, Kylie G SCHOELKOPF, Lorien JONES-WITTHUHN, Richard L JONES, Scott O |
| description | Piroplasms isolated from a farmed reindeer and elk in Wisconsin were determined to be Babesia odocoilei, based on morphology and ribosomal RNA (rRNA) analysis. Different clinical manifestations were observed in the two host species. The reindeer was parasitemic and exhibited acute babesiosis resulting in death, while the elk showed no parasites in blood smears and no overt clinical signs of babesiosis. B. odocoilei was, however, readily cultured from elk erythrocytes. Small subunit rRNA gene sequences from the two isolates were identical to that previously reported for B. odocoilei. Internal transcribed spacers 1 and 2 and 5.8S rRNA sequence analysis showed an overall identity range of 94.3-98.1% to corresponding sequences from three previously reported B. odocoilei isolates, but the Wisconsin reindeer B. odocoilei shared only 87.3% identity with a previously reported Babesia sp. isolated from a reindeer in California (RD61). |
| doi_str_mv | 10.1007/s00436-003-0984-5 |
| format | Article |
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Different clinical manifestations were observed in the two host species. The reindeer was parasitemic and exhibited acute babesiosis resulting in death, while the elk showed no parasites in blood smears and no overt clinical signs of babesiosis. B. odocoilei was, however, readily cultured from elk erythrocytes. Small subunit rRNA gene sequences from the two isolates were identical to that previously reported for B. odocoilei. Internal transcribed spacers 1 and 2 and 5.8S rRNA sequence analysis showed an overall identity range of 94.3-98.1% to corresponding sequences from three previously reported B. odocoilei isolates, but the Wisconsin reindeer B. odocoilei shared only 87.3% identity with a previously reported Babesia sp. isolated from a reindeer in California (RD61).</description><identifier>ISSN: 0932-0113</identifier><identifier>EISSN: 1432-1955</identifier><identifier>DOI: 10.1007/s00436-003-0984-5</identifier><identifier>PMID: 14505046</identifier><identifier>CODEN: PARREZ</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Animal productions ; Animals ; Arachnid Vectors ; Babesia - classification ; Babesia - genetics ; Babesia - isolation & purification ; Babesiosis - parasitology ; Babesiosis - transmission ; Babesiosis - veterinary ; Biological and medical sciences ; Erythrocytes - parasitology ; Female ; Fundamental and applied biological sciences. Psychology ; Male ; Reindeer - parasitology ; RNA, Ribosomal - genetics ; Sequence Analysis, RNA ; Terrestrial animal productions ; Ticks ; Vertebrates</subject><ispartof>Parasitology research (1987), 2003-11, Vol.91 (5), p.378-383</ispartof><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c393t-b38452b5ca75d26e557ab831b86a152212baeaba610fd0351afb5d166e89e36e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15257690$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14505046$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HOLMAN, Patricia J</creatorcontrib><creatorcontrib>BENDELE, Kylie G</creatorcontrib><creatorcontrib>SCHOELKOPF, Lorien</creatorcontrib><creatorcontrib>JONES-WITTHUHN, Richard L</creatorcontrib><creatorcontrib>JONES, Scott O</creatorcontrib><title>Ribosomal RNA analysis of Babesia odocoilei isolates from farmed reindeer (Rangifer tarandus tarandus) and elk (Cervus elaphus canadensis) in Wisconsin</title><title>Parasitology research (1987)</title><addtitle>Parasitol Res</addtitle><description>Piroplasms isolated from a farmed reindeer and elk in Wisconsin were determined to be Babesia odocoilei, based on morphology and ribosomal RNA (rRNA) analysis. Different clinical manifestations were observed in the two host species. The reindeer was parasitemic and exhibited acute babesiosis resulting in death, while the elk showed no parasites in blood smears and no overt clinical signs of babesiosis. B. odocoilei was, however, readily cultured from elk erythrocytes. Small subunit rRNA gene sequences from the two isolates were identical to that previously reported for B. odocoilei. Internal transcribed spacers 1 and 2 and 5.8S rRNA sequence analysis showed an overall identity range of 94.3-98.1% to corresponding sequences from three previously reported B. odocoilei isolates, but the Wisconsin reindeer B. odocoilei shared only 87.3% identity with a previously reported Babesia sp. isolated from a reindeer in California (RD61).</description><subject>Animal productions</subject><subject>Animals</subject><subject>Arachnid Vectors</subject><subject>Babesia - classification</subject><subject>Babesia - genetics</subject><subject>Babesia - isolation & purification</subject><subject>Babesiosis - parasitology</subject><subject>Babesiosis - transmission</subject><subject>Babesiosis - veterinary</subject><subject>Biological and medical sciences</subject><subject>Erythrocytes - parasitology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Male</subject><subject>Reindeer - parasitology</subject><subject>RNA, Ribosomal - genetics</subject><subject>Sequence Analysis, RNA</subject><subject>Terrestrial animal productions</subject><subject>Ticks</subject><subject>Vertebrates</subject><issn>0932-0113</issn><issn>1432-1955</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFUctuFDEQtBARWQIfwAX5AkoOE9zjsWfmGFY8IkVBWoE4Wu1xGwye8cbeRcqX8Ls42hU5dbW6urpVxdgrEJcgRP-uCNFJ3QghGzEOXaOesBV0sm1gVOopW4mxYgEgT9nzUn4JAb3uumfsFDollOj0iv3dBJtKmjHyze0VxwXjfQmFJ8_fo6USkCeXphQiBR5Kirijwn1OM_eYZ3I8U1gcUebnG1x-BF_RDjMubl_-g4sq7DjF3_x8TflPnVDE7c9ap3rR0VJPXvCw8O-hTKl2ywt24jEWenmsZ-zbxw9f15-bmy-frtdXN80kR7lrrBw61Vo1Ya9cq0mpHu0gwQ4aQbUttBYJLWoQ3gmpAL1VDrSmYSSpSZ6xtwfdbU53eyo7M9cXKEZcKO2L6UEBDD1UIhyIU06lZPJmm8OM-d6AMA9pmEMapqZhHtIwqu68PorvbbXqceNofyW8ORKwTBh9dWsK5ZGnWtXrUch_O66UMw</recordid><startdate>20031101</startdate><enddate>20031101</enddate><creator>HOLMAN, Patricia J</creator><creator>BENDELE, Kylie G</creator><creator>SCHOELKOPF, Lorien</creator><creator>JONES-WITTHUHN, Richard L</creator><creator>JONES, Scott O</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20031101</creationdate><title>Ribosomal RNA analysis of Babesia odocoilei isolates from farmed reindeer (Rangifer tarandus tarandus) and elk (Cervus elaphus canadensis) in Wisconsin</title><author>HOLMAN, Patricia J ; BENDELE, Kylie G ; SCHOELKOPF, Lorien ; JONES-WITTHUHN, Richard L ; JONES, Scott O</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c393t-b38452b5ca75d26e557ab831b86a152212baeaba610fd0351afb5d166e89e36e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animal productions</topic><topic>Animals</topic><topic>Arachnid Vectors</topic><topic>Babesia - classification</topic><topic>Babesia - genetics</topic><topic>Babesia - isolation & purification</topic><topic>Babesiosis - parasitology</topic><topic>Babesiosis - transmission</topic><topic>Babesiosis - veterinary</topic><topic>Biological and medical sciences</topic><topic>Erythrocytes - parasitology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Male</topic><topic>Reindeer - parasitology</topic><topic>RNA, Ribosomal - genetics</topic><topic>Sequence Analysis, RNA</topic><topic>Terrestrial animal productions</topic><topic>Ticks</topic><topic>Vertebrates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HOLMAN, Patricia J</creatorcontrib><creatorcontrib>BENDELE, Kylie G</creatorcontrib><creatorcontrib>SCHOELKOPF, Lorien</creatorcontrib><creatorcontrib>JONES-WITTHUHN, Richard L</creatorcontrib><creatorcontrib>JONES, Scott O</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology research (1987)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HOLMAN, Patricia J</au><au>BENDELE, Kylie G</au><au>SCHOELKOPF, Lorien</au><au>JONES-WITTHUHN, Richard L</au><au>JONES, Scott O</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ribosomal RNA analysis of Babesia odocoilei isolates from farmed reindeer (Rangifer tarandus tarandus) and elk (Cervus elaphus canadensis) in Wisconsin</atitle><jtitle>Parasitology research (1987)</jtitle><addtitle>Parasitol Res</addtitle><date>2003-11-01</date><risdate>2003</risdate><volume>91</volume><issue>5</issue><spage>378</spage><epage>383</epage><pages>378-383</pages><issn>0932-0113</issn><eissn>1432-1955</eissn><coden>PARREZ</coden><abstract>Piroplasms isolated from a farmed reindeer and elk in Wisconsin were determined to be Babesia odocoilei, based on morphology and ribosomal RNA (rRNA) analysis. Different clinical manifestations were observed in the two host species. The reindeer was parasitemic and exhibited acute babesiosis resulting in death, while the elk showed no parasites in blood smears and no overt clinical signs of babesiosis. B. odocoilei was, however, readily cultured from elk erythrocytes. Small subunit rRNA gene sequences from the two isolates were identical to that previously reported for B. odocoilei. Internal transcribed spacers 1 and 2 and 5.8S rRNA sequence analysis showed an overall identity range of 94.3-98.1% to corresponding sequences from three previously reported B. odocoilei isolates, but the Wisconsin reindeer B. odocoilei shared only 87.3% identity with a previously reported Babesia sp. isolated from a reindeer in California (RD61).</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>14505046</pmid><doi>10.1007/s00436-003-0984-5</doi><tpages>6</tpages></addata></record> |
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| subjects | Animal productions Animals Arachnid Vectors Babesia - classification Babesia - genetics Babesia - isolation & purification Babesiosis - parasitology Babesiosis - transmission Babesiosis - veterinary Biological and medical sciences Erythrocytes - parasitology Female Fundamental and applied biological sciences. Psychology Male Reindeer - parasitology RNA, Ribosomal - genetics Sequence Analysis, RNA Terrestrial animal productions Ticks Vertebrates |
| title | Ribosomal RNA analysis of Babesia odocoilei isolates from farmed reindeer (Rangifer tarandus tarandus) and elk (Cervus elaphus canadensis) in Wisconsin |
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